Genetic studies on ribose 5-phosphate isomerase mutants of Escherichia coli K-12.

abstract：:The Gifsy-1 phage integrates site specifically into the Salmonella chromosome via an integrase-mediated site-specific recombination mechanism. Initial genetic analysis suggests that Gifsy-1 integrase-mediated excision of the Gifsy-1 phage is influenced by proteins encoded by both the Gifsy-1 and the Gifsy-2 phages. Ou...

journal_title：Journal of bacteriology

authors： Flanigan A,Gardner JF

更新日期：2007-09-01 00:00:00

abstract：:Nine natural isolates of Escherichia coli were examined, and the sequence of the entire 1,404 bases of the gnd gene (6-phosphogluconate dehydrogenase, EC 1.1.1.44) was determined. These isolates, along with E. coli K-12, constitute 10 strains for analysis. (The sequence of the E. coli K-12 gnd gene is known.) A total ...

journal_title：Journal of bacteriology

authors： Bisercić M,Feutrier JY,Reeves PR

更新日期：1991-06-01 00:00:00

abstract：:Paenibacillus mucilaginosus is a ubiquitous functional bacterium in microbial fertilizer. Here we report the complete sequence of P. mucilaginosus 3016. Multiple sets of functional genes have been found in the genome. To the best of our knowledge, this is the first announcement about the complete genome sequence of a ...

journal_title：Journal of bacteriology

authors： Ma M,Wang Z,Li L,Jiang X,Guan D,Cao F,Chen H,Wang X,Shen D,Du B,Li J

更新日期：2012-05-01 00:00:00

abstract：:In Zymomonas mobilis, three- to fourfold more glyceraldehyde-3-phosphate dehydrogenase protein than phosphoglycerate kinase is needed for glycolysis because of differences in catalytic efficiency. Consistent with this requirement, higher levels of glyceraldehyde-3-phosphate dehydrogenase were observed with two-dimensi...

journal_title：Journal of bacteriology

authors： Eddy CK,Keshav KF,An H,Utt EA,Mejia JP,Ingram LO

更新日期：1991-01-01 00:00:00

abstract：:The alpha (62,000-dalton) and beta (49,000-dalton) subunits of Methanosarcina barkeri ATPase were purified to homogeneity. The subunits and ATPase complex were trypsinized in the presence of various nucleotides. ATP and ADP changed the trypsin sensitivity of the alpha subunit in the complex and isolated forms, suggest...

journal_title：Journal of bacteriology

authors： Inatomi K,Maeda M

更新日期：1988-12-01 00:00:00

abstract：:Prolyl-phenylalanine-specific serine protease (dentilisin) is a major extracellular protease produced by Treponema denticola. The gene, prtP, coding for the protease was recently cloned and sequenced (K. Ishihara, T. Miura, H. K. Kuramitsu, and K. Okuda, Infect. Immun. 64:5178-5186, 1996). In order to determine the ro...

journal_title：Journal of bacteriology

authors： Ishihara K,Kuramitsu HK,Miura T,Okuda K

更新日期：1998-08-01 00:00:00

abstract：:Although competence normally develops only in glucose-minimal salts media, mecA and mecB mutations permit the expression of competence and of late competence genes in complex media as well (D. Dubnau and M. Roggiani, J. Bacteriol. 172:4048-4055, 1990). The expression of late competence genes is dependent on the produc...

journal_title：Journal of bacteriology

authors： Roggiani M,Hahn J,Dubnau D

更新日期：1990-07-01 00:00:00

abstract：:Primer extension analysis of transcripts of the Bacillus subtilis autolysin (cwlB) operon indicated that SigD-dependent transcripts from the Pd promoter are missing in the degU32(Hy) and degS200 (Hy) mutants. The degU32(Hy) mutation caused a 99% reduction in the expression of a sigD-lacZ translational fusion gene cons...

journal_title：Journal of bacteriology

authors： Tokunaga T,Rashid MH,Kuroda A,Sekiguchi J

更新日期：1994-08-01 00:00:00

abstract：:A method was developed for the isolation of Neurospora bradytrophs. The bradytrophs (representing lesions in a number of pathways) were resistant to DL-p-fluorophenylalanine when growing in a leaky fashion but were sensitive when grown in the presence of their stimulating supplement. ...

journal_title：Journal of bacteriology

authors： Kinsey JA

更新日期：1979-12-01 00:00:00

abstract：:When Azotobacter vinelandii was derepressed for nitrogenase synthesis in a N-free medium containing tungstate instead of molybdate, an inactive component I was synthesized. Although this inactive component I could be activated in vivo upon addition of molybdate to the medium, it could not be activated in vitro when mo...

journal_title：Journal of bacteriology

authors： Nagatani HH,Shah VK,Brill WJ

更新日期：1974-11-01 00:00:00

abstract：:We have investigated a tyrosine kinase activity from Pseudomonas solanacearum, an economically important plant pathogen. In vitro incubation of membrane fractions with [gamma-32P]ATP and subsequent sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed an 85-kDa phosphoprotein. Phosphorylation of this prot...

journal_title：Journal of bacteriology

authors： Atkinson M,Allen C,Sequeira L

更新日期：1992-07-01 00:00:00

abstract：:Salmonella typhimurium LT2 normally expresses two acetohydroxy acid synthases (AHAS I and AHAS II). The function of AHAS I in this organism was unclear, since AHAS I-deficient (ilvBN) mutants of LT2 grew well on glucose or succinate minimal media, whereas AHAS II-deficient (ilvGM) mutants requried isoleucine for norma...

journal_title：Journal of bacteriology

authors： Dailey FE,Cronan JE Jr,Maloy SR

更新日期：1987-02-01 00:00:00

abstract：:A cloned 8.3-kilobase-pair DNA fragment carrying all the genes (iucABCD iutA) of the aerobactin iron transport system of plasmid pColV-K30 was subjected to in vitro mutagenesis to afford mutant genes iucA, iucC, and iucA iucC. Complementation analyses and identification of aerobactin precursors accumulated by Escheric...

journal_title：Journal of bacteriology

authors： de Lorenzo V,Neilands JB

更新日期：1986-07-01 00:00:00

abstract：:Although arsenic is highly toxic to most organisms, certain prokaryotes are known to grow on and respire toxic metalloids of arsenic (i.e., arsenate and arsenite). Two enzymes are known to be required for this arsenic-based metabolism: (i) the arsenate respiratory reductase (ArrA) and (ii) arsenite oxidase (AoxB). Bot...

journal_title：Journal of bacteriology

authors： Zargar K,Hoeft S,Oremland R,Saltikov CW

更新日期：2010-07-01 00:00:00

abstract：:A 22-kb segment of chromosomal DNA from Enterococcus faecalis OG1RF containing the pyrimidine biosynthesis genes pyrC and pyrD was previously detected as complementing Escherichia coli pyrC and pyrD mutations. In the present study, it was found that the E. faecalis pyrimidine biosynthetic genes in this clone (designat...

journal_title：Journal of bacteriology

authors： Li X,Weinstock GM,Murray BE

更新日期：1995-12-01 00:00:00

abstract：:The activity of beta-glucosidase (EC 3.2.1.21) in extracts of Dictyostelium discoideum was investigated. The specific activity increased early in development, declined during pseudoplasmodium formation, and increased again during sorocarp formation. The beta-glucosidase which was present in growing amoebae and during ...

journal_title：Journal of bacteriology

authors： Coston MB,Loomis WF Jr

更新日期：1969-12-01 00:00:00

abstract：:Bacillus subtilis OhrR is the prototype for the one-Cys family of organic peroxide-sensing regulatory proteins. Mutational analyses indicate that the high sensitivity of the active site cysteine (C15) to peroxidation requires three Tyr residues. Y29 and Y40 from the opposing subunit of the functional dimer hydrogen bo...

journal_title：Journal of bacteriology

authors： Soonsanga S,Fuangthong M,Helmann JD

更新日期：2007-10-01 00:00:00

abstract：:We have developed a method, using laser, optical tweezers and direct microscopic analysis of reproductive potential and membrane integrity, to assess single-cell viability in a stationary-phase Escherichia coli population. It is demonstrated here that a reduction in cell integrity, determined by using the fluorescent ...

journal_title：Journal of bacteriology

authors： Ericsson M,Hanstorp D,Hagberg P,Enger J,Nyström T

更新日期：2000-10-01 00:00:00

abstract：:We previously showed that adsorption of bacteriophages T4 and T5 to their respective outer membrane receptors induced a partial depolarization of the cytoplasmic membrane. As these membrane potential changes were independent of phage properties, we proposed that phage adsorption triggered the emission of a signal whic...

journal_title：Journal of bacteriology

authors： Letellier L,Labedan B

更新日期：1984-03-01 00:00:00

abstract：:The ribosome content was markedly reduced when illumination (at 28 C) was repeatedly interrupted; an opposite trend was observed with continuous light as the temperature was decreased. ...

journal_title：Journal of bacteriology

authors： Din GA,Gest H

更新日期：1969-03-01 00:00:00

abstract：:Welker, N. E. (Western Reserve University, Cleveland, Ohio), and L. Leon Campbell. Preferential synthesis of alpha-amylase by Bacillus stearothermophilus in the presence of 5-methyl-tryptophan. J. Bacteriol. 87:828-831. 1964.-Washed-cell suspensions of Bacillus stearothermophilus induced with pure maltose preferential...

journal_title：Journal of bacteriology

authors： WELKER NE,CAMPBELL LL

更新日期：1964-04-01 00:00:00

abstract：:The major role of RecA is thought to be in helping repair and restart stalled replication forks. During exponential growth, Bacillus subtilis recA cells exhibited few microscopically observable nucleoid defects. However, the efficiency of plating was about 12% of that of the parent strain. A substantial and additive d...

journal_title：Journal of bacteriology

authors： Sciochetti SA,Blakely GW,Piggot PJ

更新日期：2001-05-01 00:00:00

abstract：:Exfoliative toxin type B is produced by bacteriophage group II strains of Staphylococcus aureus and is a causative agent of staphylococcal scalded-skin syndrome. In addition to exfoliative toxin B, most isolates also produce a bacteriocin and are immune to the action of the bacteriocin. These phenotypes, as well as re...

journal_title：Journal of bacteriology

authors： Jackson MP,Iandolo JJ

更新日期：1986-05-01 00:00:00

abstract：:Azotobacter vinelandii was grown diazotrophically in chemostat cultures limited by sucrose, citrate, or acetate. Specific activities of cellular oxygen consumption (qO2) and nitrogenase (acetylene reduction) were measured in situ at different dilution rates (D, representing the specific growth rate mu at steady state)...

journal_title：Journal of bacteriology

authors： Kuhla J,Oelze J

更新日期：1988-11-01 00:00:00

abstract：:The lmaA gene of Listeria monocytogenes encodes a protein capable of inducing delayed-type hypersensitivity reactions in L. monocytogenes-immune mice (S. Göhmann, M. Leimeister-Wachter, E. Schiltz, W. Goebel, and T. Chakraborty, M. Microbiol. 4:1091-1099, 1990). Here we show that it is the last gene of the lma operon,...

journal_title：Journal of bacteriology

authors： Schäferkordt S,Chakraborty T

更新日期：1997-04-01 00:00:00

abstract：:Many DNA viruses concatemerize their genomes as a prerequisite to packaging into capsids. Concatemerization arises from either replication or homologous recombination. Replication is already the target of many antiviral drugs, and viral recombinases are an attractive target for drug design, particularly for combinatio...

journal_title：Journal of bacteriology

authors： Vellani TS,Myers RS

更新日期：2003-04-01 00:00:00

abstract：:Strain IMCC3088, cultivated from the Yellow Sea, is a novel isolate belonging to the OM60/NOR5 clade and is closely related to clone OM241, Congregibacter litoralis, and strain HTCC2080. Here, the genome sequence of strain IMCC3088 is presented, showing the absence of photosynthetic gene clusters and the presence of p...

journal_title：Journal of bacteriology

authors： Jang Y,Oh HM,Kang I,Lee K,Yang SJ,Cho JC

更新日期：2011-07-01 00:00:00

abstract：:Fowler, Audree V. (University of California, Los Angeles), and Irving Zabin. Effects of dimethylsulfoxide on the lactose operon in Escherichia coli. J. Bacteriol. 92:353-357. 1966.-Dimethylsulfoxide (DMSO) at a concentration of 5% (v/v) in the culture medium inhibits the growth of Escherichia coli to only a slight ext...

journal_title：Journal of bacteriology

authors： Fowler AV,Zabin I

更新日期：1966-08-01 00:00:00

abstract：:Many surface proteins of pathogenic gram-positive bacteria are linked to the cell wall envelope by a mechanism requiring a C-terminal sorting signal with an LPXTG motif. Surface proteins of Streptococcus pneumoniae harbor another motif, YSIRK-G/S, which is positioned within signal peptides. The signal peptides of some...

journal_title：Journal of bacteriology

authors： Bae T,Schneewind O

更新日期：2003-05-01 00:00:00

abstract：:We reported previously that a Salmonella enterica serovar Enteritidis dam mutant expressing a truncated Dam protein does not agglutinate in the presence of specific antibodies against O9 polysaccharide. Here we investigate the participation of Dam in lipopolysaccharide (LPS) synthesis in Salmonella. The LPS O-antigen ...

journal_title：Journal of bacteriology

authors： Sarnacki SH,Marolda CL,Noto Llana M,Giacomodonato MN,Valvano MA,Cerquetti MC

更新日期：2009-11-01 00:00:00