Isolation of subunits from Methanosarcina barkeri ATPase: nucleotide-binding site in the alpha subunit.

Abstract:

:The alpha (62,000-dalton) and beta (49,000-dalton) subunits of Methanosarcina barkeri ATPase were purified to homogeneity. The subunits and ATPase complex were trypsinized in the presence of various nucleotides. ATP and ADP changed the trypsin sensitivity of the alpha subunit in the complex and isolated forms, suggesting the presence of a nucleotide-binding site in the alpha subunit.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Inatomi K,Maeda M

doi

10.1128/jb.170.12.5960-5962.1988

subject

Has Abstract

pub_date

1988-12-01 00:00:00

pages

5960-2

issue

12

eissn

0021-9193

issn

1098-5530

journal_volume

170

pub_type

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