Location of sites that inhibit progression of replication forks in the terminus region of Escherichia coli.

Abstract:

:We used a Southern hybridization assay to locate precisely the sites at which DNA replication is arrested in the terminus region of the Escherichia coli chromosome. The assay was based on the properties of restriction fragments that contain stalled replication forks. Replication forks that entered the terminus from the clockwise direction with respect to the genetic map were inhibited near manA at a site called T2, which we located at kilobase 442 on the physical map of Bouché (J. P. Bouché, J. Mol. Biol. 154:1-20, 1982). Those that entered the terminus region traveling in the counterclockwise direction were inhibited near pyrF at a site called T1, which we located at kilobase 90. In each case we found only a single, precise site of arrest. Inhibition at T1 was not detectable in our assay in strains lacking the trans-acting locus tus, which is located near T2 and is required for T1 to function. We demonstrated that the sites of inhibition are also used during termination of replication in exponentially growing, wild-type cells. In all previous studies on the terminus of E. coli, inhibition has only been detected in strains that were modified so that the origin used was placed near the terminus to force the use of the sites of inhibition.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Pelletier AJ,Hill TM,Kuempel PL

doi

10.1128/jb.170.9.4293-4298.1988

subject

Has Abstract

pub_date

1988-09-01 00:00:00

pages

4293-8

issue

9

eissn

0021-9193

issn

1098-5530

journal_volume

170

pub_type

杂志文章
  • PHAGE ISOLATED FROM LYSOGENIC BACILLUS ANTHRACIS.

    abstract::Buck, C. A. (Kansas State University, Manhattan), R. L. Anacker, F. S. Newman, and A. Eisenstark. Phage isolated from lysogenic Bacillus anthracis. J. Bacteriol. 85:1423-1430. 1963.-Bacillus anthracis cultures (a total of 311) were obtained from several laboratories throughout the world; 139 of these were screened for...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.85.6.1423-1430.1963

    authors: BUCK CA,ANACKER RL,NEWMAN FS,EISENSTARK A

    更新日期:1963-06-01 00:00:00

  • Identification of a novel nutrient-sensing histidine kinase/response regulator network in Escherichia coli.

    abstract::When carbon sources become limiting for growth, bacteria must choose which of the remaining nutrients should be used first. We have identified a nutrient-sensing signaling network in Escherichia coli that is activated at the transition to stationary phase. The network is composed of the two histidine kinase/response r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01554-14

    authors: Behr S,Fried L,Jung K

    更新日期:2014-06-01 00:00:00

  • Mechanisms of biodegradation of metal-citrate complexes by Pseudomonas fluorescens.

    abstract::Biodegradation of metal-citrate complexes by Pseudomonas fluorescens depends on the nature of the complex formed between the metal and citric acid. Bidentate Fe(III)-, Ni-, and Zn-citrate complexes were readily biodegraded, but the tridentate Cd- and Cu-citrate, and U-citrate complexes were not. The biodegradation of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.8.1989-1993.1995

    authors: Joshi-Tope G,Francis AJ

    更新日期:1995-04-01 00:00:00

  • Influence of SV40 Genome on the Replication of an Adenovirus-SV40 "Hybrid" Population.

    abstract::Feldman, L. A. (Baylor University College of Medicine, Houston, Tex.), J. L. Melnick, and F. Rapp. Influence of SV40 genome on the replication of an adenovirus-SV40 "hybrid" population. J. Bacteriol. 90:778-782. 1965.-Replication of a type 7 adenovirus-SV40 hybrid population in primary African green monkey kidney cell...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.90.3.778-782.1965

    authors: Feldman LA,Melnick JL,Rapp F

    更新日期:1965-09-01 00:00:00

  • Isolation and purification of Flavobacterium alpha-1,3-glucanase-hydrolyzing, insoluble, sticky glucan of Streptococcus mutans.

    abstract::Studies were made on the physical and chemical properties of polysaccharides synthesized by cell-free extracts of Streptococcus mutans, Streptococcus sanguis, and Streptococcus sp. and their susceptibilities to dextranases. Among the polysaccharides examined, insoluble glucans were rather resistant to available dextra...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.124.3.1489-1501.1975

    authors: Ebisu S,Kato K,Kotani S,Misaki A

    更新日期:1975-12-01 00:00:00

  • Bacteriocin protein BacL1 of Enterococcus faecalis targets cell division loci and specifically recognizes L-Ala2-cross-bridged peptidoglycan.

    abstract::Bacteriocin 41 (Bac41) is produced from clinical isolates of Enterococcus faecalis and consists of two extracellular proteins, BacL1 and BacA. We previously reported that BacL1 protein (595 amino acids, 64.5 kDa) is a bacteriolytic peptidoglycan D-isoglutamyl-L-lysine endopeptidase that induces cell lysis of E. faecal...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02203-14

    authors: Kurushima J,Nakane D,Nishizaka T,Tomita H

    更新日期:2015-01-01 00:00:00

  • Transcriptional organization of the czc heavy-metal homeostasis determinant from Alcaligenes eutrophus.

    abstract::The Czc system of Alcaligenes eutrophus mediates resistance to cobalt, zinc, and cadmium through ion efflux catalyzed by the CzcCB2A cation-proton antiporter. DNA sequencing of the region upstream of the czcNICBADRS determinant located on megaplasmid pMOL30 revealed the 5' end of czcN and a gene for a MgtC-like protei...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.8.2385-2393.1999

    authors: Grosse C,Grass G,Anton A,Franke S,Santos AN,Lawley B,Brown NL,Nies DH

    更新日期:1999-04-01 00:00:00

  • Purification of the Escherichia coli purine regulon repressor and identification of corepressors.

    abstract::The Escherichia coli pur regulon repressor protein was overproduced in a phage T7 expression system. The overexpressed repressor constituted approximately 35% of the soluble cellular protein. Pur repressor was purified to near homogeneity by two chromatographic steps. Hypoxanthine or guanine was required for binding o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.10.5637-5642.1990

    authors: Rolfes RJ,Zalkin H

    更新日期:1990-10-01 00:00:00

  • Identification of five new essential genes involved in the synthesis of a secreted protein in Escherichia coli.

    abstract::To define additional components of the export machinery of Escherichia coli, I have isolated extragenic suppressors of a mutant [secA(Ts)] that is temperature sensitive for growth and secretion at 37 degrees C. Suppressors that restored growth at 37 degrees C, but that rendered the cell cold sensitive for growth at 28...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.161.1.285-291.1985

    authors: Oliver DB

    更新日期:1985-01-01 00:00:00

  • Pyrimidine biosynthesis in Lactobacillus leichmannii.

    abstract::Tracer studies of pyrimidine biosynthesis in Lactobacillus leichmannii (ATCC 7830) indicated that, while aspartate is utilized in the usual manner, the guanido carbon of arginine, rather than carbon dioxide, is utilized as a pyrimidine precursor. The guanido carbon of arginine also contributes, to some extent, to the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.4.1249-1254.1968

    authors: Hutson JY,Downing M

    更新日期:1968-10-01 00:00:00

  • Genetic mapping of katG, a locus that affects synthesis of the bifunctional catalase-peroxidase hydroperoxidase I in Escherichia coli.

    abstract::A locus unlinked to either katE or katF that affected catalase levels in Escherichia coli was identified and localized between metB and ppc at 89.2 min on the genome. The locus was named katG. Mutations in katG which prevented the formation of both isoenzyme forms of the bifunctional catalase-peroxidase HPI were creat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.2.661-667.1985

    authors: Loewen PC,Triggs BL,George CS,Hrabarchuk BE

    更新日期:1985-05-01 00:00:00

  • Topological model of the Rhodobacter capsulatus light-harvesting complex I assembly protein LhaA (previously known as ORF1696).

    abstract::A theoretical topology of the Rhodobacter capsulatus membrane protein LhaA was formulated and evaluated by gene fusion experiments. The apparent topological locations of fusion enzymes were compared with the theoretically derived structure, and a model of LhaA is suggested that consists of 12 transmembrane segments, w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.17.4742-4745.1998

    authors: Young CS,Beatty JT

    更新日期:1998-09-01 00:00:00

  • Novel mutation to dominant fucose resistance in the L-arabinose operon of Escherichia coli.

    abstract::We isolated an unusual mutant in which the arabinose operon carried an araC lesion that led to a dominant fucose resistance while retaining recessive constitutivity. All previously characterized C(c) mutations have been recessive for both fucose resistance and constitutivity. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.115.2.711-713.1973

    authors: Nathanson NM,Schleif R

    更新日期:1973-08-01 00:00:00

  • Control of Escherichia coli growth by CO2.

    abstract::Escherichia coli B dependence on CO2 for growth was demonstrated. At suboptimal CO2 concentrations the rate of growth was controlled by CO2 concentration. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.135.3.1162-1164.1978

    authors: Repaske R,Clayton MA

    更新日期:1978-09-01 00:00:00

  • Fine structure of Vibrio cholerae during toxin production.

    abstract::The fine structural changes associated with cell growth and toxin production have been examined in Vibrio cholerae strain 569B. No morphological alterations in the cell envelope are apparent during logarithmic growth with thin-section techniques. However, internal swelling, suggesting alteration of cell envelope perme...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.3.1393-1401.1969

    authors: Kennedy JR,Richardson SH

    更新日期:1969-12-01 00:00:00

  • NEW TYPE OF STREPTOMYCIN RESISTANCE RESULTING FROM ACTION OF THE EPISOMELIKE MUTATOR FACTOR IN ESCHERICHIA COLI.

    abstract::Gundersen, Wenche B. (Oslo University, Oslo, Norway). New type of streptomycin resistance resulting from action of the episomelike mutator factor in Escherichia coli. J. Bacteriol. 86:510-516. 1963.-Analyses have been performed to elucidate the genetic nature of the streptomycin resistance that results from the action...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.86.3.510-516.1963

    authors: GUNDERSEN WB

    更新日期:1963-09-01 00:00:00

  • Analysis of tnrA alleles which result in a glucose-resistant sporulation phenotype in Bacillus subtilis.

    abstract::Bacillus subtilis cells cannot sporulate in the presence of catabolites such as glucose. During the analysis of Tn10-generated mutants, we found that deletion of the C-terminal region of the tnrA gene, which encodes a global regulator that positively regulates a number of genes in response to nitrogen limitation, resu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.17.5009-5012.2000

    authors: Shin BS,Choi SK,Smith I,Park SH

    更新日期:2000-09-01 00:00:00

  • Mutations in the araC regulatory gene of Escherichia coli B/r that affect repressor and activator functions of AraC protein.

    abstract::Mutations in the araC gene of Escherichia coli B/r were isolated which alter both activation of the araBAD operon expression and autoregulation. The mutations were isolated on an araC-containing plasmid by hydroxylamine mutagenesis of plasmid DNA. The mutant phenotype selected was the inability to autoregulate. The DN...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.166.3.892-900.1986

    authors: Cass LG,Wilcox G

    更新日期:1986-06-01 00:00:00

  • Effect of temperature on the distribution of membrane particles in Streptococcus faecalis as seen by the freeze-fracture technique.

    abstract::When cells of Streptococcus faecalis ATCC 9790 were incubated at temperatures above 10 C before being frozen for freeze-fracture, a random distribution of particles was observed on the outer fracture face of the freeze-cleaved cell membrane. However, when cells were incubated below 10 C before freezing, particleless p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.118.2.725-734.1974

    authors: Tsien HC,Higgins ML

    更新日期:1974-05-01 00:00:00

  • Glucose-triggered germination of Bacillus megaterium spores.

    abstract::Triggering of germination in Bacillus megaterium QM B1551 spores with D-glucose was studied. First, the interaction of glucose with spores for less than 1 min resulted in triggering almost 90% of the spores after the glucose was removed by dilution. Therefore only a brief time is needed for glucose to trigger germinat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.138.2.442-445.1979

    authors: Racine FM,Dills SS,Vary JC

    更新日期:1979-05-01 00:00:00

  • Identification of Treponema pallidum penicillin-binding proteins.

    abstract::Penicillin-binding proteins of 180, 89, 80, 68, 61, 41, and 38 kilodaltons were identified in Treponema pallidum (Nichols) by their covalent binding of [35S]benzylpenicillin. Penicillin-binding proteins are localized in the plasma membranes of many bacterial species and may serve as useful markers for determining plas...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.11.5298-5300.1987

    authors: Cunningham TM,Miller JN,Lovett MA

    更新日期:1987-11-01 00:00:00

  • Genome sequence of Enterococcus sp. strain C1, an azo dye decolorizer.

    abstract::Enterococcus sp. strain C1 is a facultative anaerobe which was coisolated with Citrobacter sp. strain A1 from a sewage oxidation pond. Strain C1 could degrade azo dyes very efficiently via azo reduction and desulfonation in a microaerophilic environment. Here the draft genome sequence of Enterococcus sp. C1 is reporte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01372-12

    authors: Chan GF,Gan HM,Rashid NA

    更新日期:2012-10-01 00:00:00

  • Reassociation of deoxyribonucleic acids from Actinoplanes and other actinomycetes.

    abstract::The ability of deoxyribonucleic acid (DNA) isolated from a number of actinomycetes to reassociate with reference DNA from Actinoplanes philippinensis or Streptomyces venezuelae has been measured. All of the DNA preparations except for those from Nocardia erythropolis and Thermomonospora viridis contained 70 to 73 mole...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.102.1.30-35.1970

    authors: Farina G,Bradley SG

    更新日期:1970-04-01 00:00:00

  • Binding of SycH chaperone to YscM1 and YscM2 activates effector yop expression in Yersinia enterocolitica.

    abstract::Yersinia enterocolitica transports YscM1 and YscM2 via the type III pathway, a mechanism that is required for the establishment of bacterial infections. Prior to host cell contact, YscM1 and YscM2 exert posttranscriptional regulation to inhibit expression of effector yop genes, which encode virulence factors that trav...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.3.829-841.2004

    authors: Cambronne ED,Sorg JA,Schneewind O

    更新日期:2004-02-01 00:00:00

  • Instability of Rickettsia prowazekii RNA polymerase-promoter complexes.

    abstract::The Rickettsia prowazekii sigma factor was overexpressed, purified, and used to reconstitute RNA polymerase holoenzyme species. R. prowazekii RNA polymerase-promoter complexes were unstable and remained dissociable and heparin sensitive under conditions in which the corresponding Escherichia coli complexes were not. T...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.21.6301-6303.1995

    authors: Aniskovitch LP,Winkler HH

    更新日期:1995-11-01 00:00:00

  • Comparative study of the events associated with colicin induction.

    abstract::Colicinogenic factors ColI and ColV, which have been shown to behave as sex factors, could not be induced with mitomycin C. In contrast, the ColE(1), ColE(2), and ColE(3) factors, which do not exhibit any fertility factor characteristics, are inducible by this agent. The induced production of colicins E(1), E(2), and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.3.691-699.1967

    authors: Herschman HR,Helinski DR

    更新日期:1967-09-01 00:00:00

  • Transfer among Erwinia spp. and other enterobacteria of antibiotic resistance carried on R factors.

    abstract::Antibiotic resistance carried on R factors was transferred by conjugation from Escherichia coli B/r and Shigella flexneri 1a to Erwinia spp. Tetracycline resistance (TetR) carried on R factor R100 drd-56 was transferred from E. coli B/r to strains of Erwinia amylovora, E. aroideae, E. atroseptica, E. chrysanthemi, E. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.1.576-584.1972

    authors: Chatterjee AK,Starr MP

    更新日期:1972-10-01 00:00:00

  • Imprecise excision of plasmid pE194 from the chromosomes of Bacillus subtilis pE194 insertion strains.

    abstract::Plasmid pE194 has been shown to be rescued by integration after cultivation of infected Bacillus subtilis recE4 cells at a restrictive high temperature. The plasmid is also spontaneously excised from the chromosome at a low frequency by precise or imprecise excision (J. Hofemeister, M. Israeli-Reches, and D. Dubnau, M...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.21.6997-7002.1992

    authors: Conrad B,Bashkirov VI,Hofemeister J

    更新日期:1992-11-01 00:00:00

  • Transcriptional regulation of delta-aminolevulinic acid dehydratase synthesis by oxygen in Bradyrhizobium japonicum and evidence for developmental control of the hemB gene.

    abstract::An increased demand for cytochromes is associated with symbiotic development and microaerobic metabolism in the bacterium Bradyrhizobium japonicum, and evidence suggests that hemB, rather than hemA, is the first essential bacterial heme synthesis gene in symbiosis with soybean. Steady-state levels of mRNA and protein ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.11.3706-3710.1997

    authors: Chauhan S,O'Brian MR

    更新日期:1997-06-01 00:00:00

  • Antibacterial nitroacridine, Nitroakridin 3582: binding to nucleic acids in vitro and effects on selected cell-free model systems of macromolecular biosynthesis.

    abstract::Nitroakridin 3582 (NA) formed complexes with native deoxyribonucleic acid (DNA) and with transfer ribonucleic acid (tRNA) species from Escherichia coli. Spectrophotometric titrations of NA with these nucleic acids produced numerical results from which nonlinear adsorption isotherms were derived. These curves indicated...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.108.3.1026-1033.1971

    authors: Wolfe AD,Cook TM,Hahn FE

    更新日期:1971-12-01 00:00:00