Analysis of tnrA alleles which result in a glucose-resistant sporulation phenotype in Bacillus subtilis.

Abstract:

:Bacillus subtilis cells cannot sporulate in the presence of catabolites such as glucose. During the analysis of Tn10-generated mutants, we found that deletion of the C-terminal region of the tnrA gene, which encodes a global regulator that positively regulates a number of genes in response to nitrogen limitation, results in a catabolite-resistant sporulation phenotype. Analyses of nrg-lacZ and nasB-lacZ, which are activated by TnrA under nitrogen limitation, showed that C-terminally truncated TnrA activates nitrogen-regulated genes constitutively. The relief of catabolite repression of sporulation may result from the uncontrolled expression of the TnrA-regulated genes.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Shin BS,Choi SK,Smith I,Park SH

doi

10.1128/jb.182.17.5009-5012.2000

keywords:

subject

Has Abstract

pub_date

2000-09-01 00:00:00

pages

5009-12

issue

17

eissn

0021-9193

issn

1098-5530

journal_volume

182

pub_type

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