Molecular cloning and regulation of expression of the genes for initiation factor 3 and two aminoacyl-tRNA synthetases.

Abstract:

:A 22-kilobase fragment of the Escherichia coli chromosome which contains the genes for translation initiation factor 3, phenylalanyl-tRNA synthetase, and threonyl-tRNA synthetase was cloned into plasmid pACYC184. The hybrid plasmid (designated pID1) complements a temperature-sensitive pheS lesion in E. coli NP37. pID1-transformed NP37 overproduce initiation factor 3 and phenylalanyl-tRNA synthetase. Gene expression from pID1 was studied in vitro in a coupled transcription-translation system and in minicells. The results suggest that the genes for initiation factor 3 and phenylalanyl- and threonyl-tRNA synthetase are regulated by different mechanisms.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Elseviers D,Gallagher P,Hoffman A,Weinberg B,Schwartz I

subject

Has Abstract

pub_date

1982-10-01 00:00:00

pages

357-62

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

152

pub_type

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