Abstract:
:The filamentous bacterium Streptomyces coelicolor forms an aerial mycelium as a prerequisite to sporulation, which occurs in the aerial hyphae. Uncontrolled activity of the extracytoplasmic function sigma factor sigmaU blocks the process of aerial mycelium formation in this organism. Using a green fluorescent protein transcriptional reporter, we have demonstrated that sigU transcription is autoregulated. We have defined a sigmaU-dependent promoter sequence and used this to identify 22 likely sigmaU regulon members in the S. coelicolor genome. Since many of these genes encode probable secreted proteins, we characterized the extracellular proteome of a mutant with high sigmaU activity caused by disruption of rsuA, the presumed cognate anti-sigma factor of sigmaU. This mutant secreted a much greater quantity and diversity of proteins than the wild-type strain. Peptide mass fingerprinting was used to identify 79 proteins from the rsuA mutant culture supernatant. The most abundant species, SCO2217, SCO0930, and SCO2207, corresponded to secreted proteins or lipoproteins of unknown functions whose genes are in the proposed sigmaU regulon. Several unique proteases were also detected in the extracellular proteome of the mutant, and the levels of the protease inhibitor SCO0762 were much reduced compared to those of the wild type. Consequently, extracellular protease activity was elevated about fourfold in the rsuA mutant. The functions of the proteins secreted as a result of sigmaU activity may be important for combating cell envelope stress and modulating morphological differentiation in S. coelicolor.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Gordon ND,Ottaviano GL,Connell SE,Tobkin GV,Son CH,Shterental S,Gehring AMdoi
10.1128/JB.01759-07subject
Has Abstractpub_date
2008-02-01 00:00:00pages
894-904issue
3eissn
0021-9193issn
1098-5530pii
JB.01759-07journal_volume
190pub_type
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更新日期:1974-05-01 00:00:00
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更新日期:1987-08-01 00:00:00
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更新日期:1974-02-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1962-11-01 00:00:00
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更新日期:1996-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1982-12-01 00:00:00
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更新日期:1999-06-01 00:00:00