A defect in the acetyl coenzyme Aacetate pathway poisons recombinational repair-deficient mutants of Escherichia coli.

abstract：:DNA-binding assays using mobility shift polyacrylamide gel electrophoresis revealed the presence of a protein that specifically bound to a restriction fragment -288 to -191 bp upstream from the transcriptional start point of strR, a regulatory gene for streptomycin biosynthesis in Streptomyces griseus. The binding sit...

journal_title：Journal of bacteriology

authors： Vujaklija D,Horinouchi S,Beppu T

更新日期：1993-05-01 00:00:00

abstract：:Purified Rickettsia prowazekii cells were able to transport L-proline. The influx of this amino acid had a Kt of 14 microM and a Vmax of about 64 pmol/min per mg of protein. Proline could not be transported by heat-killed or metabolically poisoned rickettsiae or at 0 degrees C. The uptake of proline was linear for alm...

journal_title：Journal of bacteriology

authors： Winkler HH,Daugherty RM

更新日期：1984-05-01 00:00:00

abstract：:This paper reports the partial characterization of two temperature-sensitive mutants of Escherichia coli with alterations in ribosomal ribonucleic acid (rRNA) metabolism at the restrictive temperature. Both mutants continue to synthesize deoxyribonucleic acid and protein at 42 C but showed little or no accumulation of...

journal_title：Journal of bacteriology

authors： Jackson JM 3rd,Chaney SG

更新日期：1976-03-01 00:00:00

abstract：:lac operon fusions to DNA damage-inducible (din) loci were generated in Salmonella typhimurium LT2. Many of these din fusions were efficiently repressed by cloned Escherichia coli LexA, while others were not; all required RecA for induction. Several din fusions exhibited strong inducibility and will be useful in devel...

journal_title：Journal of bacteriology

authors： Smith CM,Arany Z,Orrego C,Eisenstadt E

更新日期：1991-06-01 00:00:00

abstract：:The first alkaline phosphatase (APase) structural gene mutant of Bacillus subtilis 168 was constructed by using a clone identified by hybridization to a synthetic degenerative oligonucleotide. The design of the probe was based on the first 29 amino acids of the sequenced mature APase III protein, which had been isolat...

journal_title：Journal of bacteriology

authors： Bookstein C,Edwards CW,Kapp NV,Hulett FM

更新日期：1990-07-01 00:00:00

abstract：:A fusion constructed between the putative attenuator plus the first 219 nucleotides of the ermC (erythromycin resistance) structural gene and a 5' terminally deleted lacZ gene produced a moderate, basal level of beta-galactosidase which was increased by erythromycin addition. Another construction containing an intact ...

journal_title：Journal of bacteriology

authors： Kirsch DR,Lai MH

更新日期：1984-07-01 00:00:00

abstract：:A cosmid library of copper-resistant (Cur) Pseudomonas syringae pv. tomato PT23 plasmid DNA was constructed and mobilized into the copper-sensitive recipient P. syringae pv. syringae PS61. One resultant cosmid clone, pCOP1 (46 kilobases), conferred copper resistance. The PT23 Cur gene(s) was located on pCOP1 by subclo...

journal_title：Journal of bacteriology

authors： Bender CL,Cooksey DA

更新日期：1987-02-01 00:00:00

abstract：:We describe the isolation and characterization of amber mutations in the lexA gene of Escherichia coli K-12. These mutations, designated spr(Am), were isolated and characterized in a lexA tif sfi genetic background. They abolished the sensitivity of the strain to UV light and resulted in high rates of synthesis of rec...

journal_title：Journal of bacteriology

authors： Pacelli LZ,Edmiston SH,Mount DW

更新日期：1979-01-01 00:00:00

abstract：:A DNA sequence (brpA) which regulates the expression of the genes of the bialaphos biosynthesis pathway (bap) in Streptomyces hygroscopicus was identified and characterized. A newly isolated nonproducing mutant (NP57) had a pleiotropic defect involving at least 6 of the 13 known bap genes; only the step 6 conversion c...

journal_title：Journal of bacteriology

authors： Anzai H,Murakami T,Imai S,Satoh A,Nagaoka K,Thompson CJ

更新日期：1987-08-01 00:00:00

abstract：:Plasmid Rms312, specifying resistance to tetracycline (Tc), chloramphenicol (Cm), streptomycin (Sm), sulfonamide (Su), and mercury chloride (Mer), deletes both Tc and Cm Sm Su Mer determinants at a high frequency in Salmonella typhimurium LT2. S. typhimurium mutants that were stable carriers of Rms312 were isolated by...

journal_title：Journal of bacteriology

authors： Watanabe H,Hashimoto H,Mitsuhashi S

更新日期：1980-04-01 00:00:00

abstract：:Disrupted spheroplast preparations of Pseudomonas schuylkilliensis strain P contained fragments of cytoplasmic membrane and approximately 82% of the total cellular phospholipid. The protoplast-bursting factor (PB-factor), partially purified from pig pancreas, and a heat-treated pancreatic lipase fraction both inhibite...

journal_title：Journal of bacteriology

authors： Mizuno S,Maruo B

更新日期：1972-01-01 00:00:00

abstract：:Gundersen, Wenche B. (Oslo University, Oslo, Norway). New type of streptomycin resistance resulting from action of the episomelike mutator factor in Escherichia coli. J. Bacteriol. 86:510-516. 1963.-Analyses have been performed to elucidate the genetic nature of the streptomycin resistance that results from the action...

journal_title：Journal of bacteriology

authors： GUNDERSEN WB

更新日期：1963-09-01 00:00:00

abstract：:Dehalogenases are key enzymes in the metabolism of halo-organic compounds. This paper describes a systematic approach to the isolation and molecular analysis of two families of bacterial alpha-halocarboxylic acid (alphaHA) dehalogenase genes, called group I and group II deh genes. The two families are evolutionarily u...

journal_title：Journal of bacteriology

authors： Hill KE,Marchesi JR,Weightman AJ

更新日期：1999-04-01 00:00:00

abstract：:In this report, we show that biofilm formation by Streptococcus pneumoniae serotype 19 gives rise to variants (the small mucoid variant [SMV] and the acapsular small-colony variant [SCV]) differing in capsule production, attachment, and biofilm formation compared to wild-type strains. All biofilm-derived variants harb...

journal_title：Journal of bacteriology

authors： Allegrucci M,Sauer K

更新日期：2008-10-01 00:00:00

abstract：:Paenibacillus mucilaginosus is a ubiquitous functional bacterium in microbial fertilizer. Here we report the complete sequence of P. mucilaginosus 3016. Multiple sets of functional genes have been found in the genome. To the best of our knowledge, this is the first announcement about the complete genome sequence of a ...

journal_title：Journal of bacteriology

authors： Ma M,Wang Z,Li L,Jiang X,Guan D,Cao F,Chen H,Wang X,Shen D,Du B,Li J

更新日期：2012-05-01 00:00:00

abstract：:By partial acid hydrolysis, methylation and gas-liquid chromatography-mass spectrometry of the methylated monomers (as the alditol acetates), mass spectrometry of trimethylsilylated disaccharide alditols, as well as proton magnetic resonance, the primary structure of the Klebsiella serotype 25 capsular polysaccharide ...

journal_title：Journal of bacteriology

authors： Niemann H,Kwiatkowski B,Westphal U,Stirm S

更新日期：1977-04-01 00:00:00

abstract：:The protobranch bivalve Solemya velum Say (Mollusca: Bivalvia) houses chemoautotrophic symbionts intracellularly within its gills. These symbionts were characterized through sequencing of polymerase chain reaction-amplified 16S rRNA coding regions and hybridization of an Escherichia coli gene probe to S. velum genomic...

journal_title：Journal of bacteriology

authors： Eisen JA,Smith SW,Cavanaugh CM

更新日期：1992-05-01 00:00:00

abstract：:Deoxycytosine methylase (Dcm) enzyme activity causes mutagenesis in vitro either directly by enzyme-induced deamination of cytosine to uracil in the absence of the methyl donor, S-adenosylmethionine (SAM), or indirectly through spontaneous deamination of [5-methyl]cytosine to thymine. Using a Lac reversion assay, we i...

journal_title：Journal of bacteriology

authors： Macintyre G,Atwood CV,Cupples CG

更新日期：2001-02-01 00:00:00

abstract：:A 3.3-kilobase PvuII fragment carrying the PHR1 gene of Saccharomyces cerevisiae has been cloned into an Escherichia coli expression vector and introduced into E. coli strains deficient in DNA photolyase. Complementation of the E. coli phr-1 mutation was observed, strongly suggesting that the yeast PHR1 gene encodes a...

journal_title：Journal of bacteriology

authors： Sancar GB

更新日期：1985-02-01 00:00:00

abstract：:Genome duplication and segregation normally are completed before cell division in all organisms. The temporal relation of duplication and segregation, however, can vary in bacteria. Chromosomal regions can segregate towards opposite poles as they are replicated or can stay cohered for a considerable period before segr...

journal_title：Journal of bacteriology

authors： Srivastava P,Fekete RA,Chattoraj DK

更新日期：2006-02-01 00:00:00

abstract：:An endonuclease was detected in strains of Salmonella typhimurium containing the drug resistance plasmid pKM101. The enzyme was not detectable in strains lacking this plasmid, but it was present in strains containing mutants of pKM101 that were no longer able to enhance host cell mutagenesis. The endonuclease had a mo...

journal_title：Journal of bacteriology

authors： Lackey D,Walker GC,Keng T,Linn S

更新日期：1977-08-01 00:00:00

abstract：UNLABELLED:Streptococcus pneumoniae is able to integrate exogenous DNA into its genome by natural genetic transformation. Transient accumulation of high levels of the only S. pneumoniae alternative σ factor is insufficient for development of full competence without expression of a second competence-specific protein, Co...

journal_title：Journal of bacteriology

authors： Tovpeko Y,Bai J,Morrison DA

更新日期：2016-08-11 00:00:00

abstract：:A polA12 recA718 double mutant of Escherichia coli, in which DNA polymerase I is temperature sensitive, was unable to maintain normal DNA synthesis or to form colonies on rich media at 42 degrees C. Overproduction of DnaE protein, the polymerizing alpha subunit of DNA polymerase III, restored bacterial DNA replication...

journal_title：Journal of bacteriology

authors： Witkin EM,Roegner-Maniscalco V

更新日期：1992-06-01 00:00:00

abstract：:The effect of dihydrostreptomycin on the incorporation of amino acids into protein in antibiotic-deprived cells of a streptomycin-dependent strain of Escherichia coli B has been compared with its effect on protein synthesis in extracts from cells of the same strain. Stimulation of phenylalanine incorporation into prot...

journal_title：Journal of bacteriology

authors： Dixon H,Polglase WJ

更新日期：1969-10-01 00:00:00

abstract：:Shigella flexneri is a gram-negative bacterium that causes diarrhea and dysentery by invasion and spread through the colonic epithelium. Bacteria spread by assembling actin and other cytoskeletal proteins of the host into "actin tails" at the bacterial pole; actin tail assembly provides the force required to move bact...

journal_title：Journal of bacteriology

authors： Charles M,Magdalena J,Theriot JA,Goldberg MB

更新日期：1999-02-01 00:00:00

abstract：:A systematic survey for the presence of plasmids in 17 different xenobiotic-degrading Sphingomonas strains was performed. In almost all analyzed strains, two to five plasmids with sizes of about 50 to 500 kb were detected by using pulsed-field gel electrophoresis. A comparison of plasmid preparations untreated or trea...

journal_title：Journal of bacteriology

authors： Basta T,Keck A,Klein J,Stolz A

更新日期：2004-06-01 00:00:00

abstract：:Several spontaneous cya and crp mutants of Escherichia coli have been selected as clones simultaneously resistant to phage lambda and nalidixic acid and characterized. Both cya and crp mutants have been found to grow as cocci with increased doubling times. They have increased resistance to some mutagens (methylmethane...

journal_title：Journal of bacteriology

authors： Kumar S

更新日期：1976-02-01 00:00:00

abstract：:Staphylococcus aureus responds to changing extracellular environments in part by adjusting its proteome through alterations of transcriptional priorities and selective degradation of the preexisting pool of proteins. In Bacillus subtilis, the proteolytic adaptor protein MecA has been shown to play a role in assisting ...

journal_title：Journal of bacteriology

authors： Donegan NP,Marvin JS,Cheung AL

更新日期：2014-12-01 00:00:00

abstract：:HtrA-type serine proteases participate in folding and degradation of aberrant proteins and in processing and maturation of native proteins. Mutation of the corresponding genes often confers a pleiotropic phenotype that can include temperature sensitivity, sensitivity to osmotic and oxidative stress, and attenuated vir...

journal_title：Journal of bacteriology

authors： Noone D,Howell A,Collery R,Devine KM

更新日期：2001-01-01 00:00:00

abstract：:The copy number of 2 mu DNA-derived plasmids in CIR+ Saccharomyces cerevisiae transformants is determined by its selective marker and is usually much lower than that of the endogenous plasmid. Only plasmids containing the leu2 allele of pJDB219, designated as leu2-d, under selective conditions displayed a higher copy ...

journal_title：Journal of bacteriology

authors： Erhart E,Hollenberg CP

更新日期：1983-11-01 00:00:00