Abstract:
:The protobranch bivalve Solemya velum Say (Mollusca: Bivalvia) houses chemoautotrophic symbionts intracellularly within its gills. These symbionts were characterized through sequencing of polymerase chain reaction-amplified 16S rRNA coding regions and hybridization of an Escherichia coli gene probe to S. velum genomic DNA restriction fragments. The symbionts appeared to have only one copy of the 16S rRNA gene. The lack of variability in the 16S sequence and hybridization patterns within and between individual S. velum organisms suggested that one species of symbiont is dominant within and specific for this host species. Phylogenetic analysis of the 16S sequences of the symbionts indicates that they lie within the chemoautotrophic cluster of the gamma subdivision of the eubacterial group Proteobacteria.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Eisen JA,Smith SW,Cavanaugh CMdoi
10.1128/jb.174.10.3416-3421.1992keywords:
subject
Has Abstractpub_date
1992-05-01 00:00:00pages
3416-21issue
10eissn
0021-9193issn
1098-5530journal_volume
174pub_type
杂志文章abstract::SpoVM is an unusually small (26-residue-long) protein that is produced in the mother cell chamber of the sporangium during the process of sporulation in Bacillus subtilis. We investigated the subcellular localization of SpoVM, which is believed to be an amphipathic alpha-helix, by using a fusion of the sporulation pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.4.1391-1398.2003
更新日期:2003-02-01 00:00:00
abstract::A method of assay of immunogenic potency of the cholera gut toxin is described; it is based on the relation of dose of antigen to neutralizing antibody titer produced in the rabbit under defined conditions and allows quantification of immunogenicity as immunogenic units per milligram of protein. Evidence, based on imm...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.2.985-993.1969
更新日期:1969-11-01 00:00:00
abstract::An apparatus has been developed in which bacterial growth can be measured very precisely over short intervals of time. Its precision is presented and used to assess the constancy of growth in batch culture. Under certain conditions, i.e., Luria broth or 0.2% glucose-M9 medium at very low cell densities, the specific g...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.3.969-975.1978
更新日期:1978-12-01 00:00:00
abstract::The two closely linked fdhD and fdhE genes of Escherichia coli are required for the formation of active membrane-bound phenazine methosulfate-linked formate dehydrogenase (FDH-PMS). Both genes were isolated from a cosmid library. Restriction endonuclease analysis associated with Mu dII1734 insertion mutagenesis indica...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.10.6112-6121.1990
更新日期:1990-10-01 00:00:00
abstract::Video techniques were used to record changes in motility of cells of Streptococcus sp. strain V4051 exposed to a variety of protein modification reagents. Starved cells were tethered to glass by a single flagellum, energized metabolically with glucose, or treated with valinomycin and energized artificially via shifts ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.3.832-843.1984
更新日期:1984-06-01 00:00:00
abstract::Spo0A is a DNA binding protein in Bacillus subtilis required for the activation of spoIIG and other promoters at the onset of endospore formation. Activation of some of these promoters may involve interaction of Spo0A and the sigmaA subunit of RNA polymerase. Previous studies identified two single-amino-acid substitut...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.14.3578-3583.1998
更新日期:1998-07-01 00:00:00
abstract::Methanohalophilus strain FDF1 synthesizes beta-glutamine, betaine, and N epsilon-acetyl-beta-lysine as osmoprotective agents when the cells are grown in high external concentrations of NaCl. Nuclear magnetic resonance spectroscopic analyses of 13CH3OH-12CO2 label incorporation by the cells provide information on the b...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.20.6688-6693.1992
更新日期:1992-10-01 00:00:00
abstract::Primer extension analysis of transcripts of the Bacillus subtilis autolysin (cwlB) operon indicated that SigD-dependent transcripts from the Pd promoter are missing in the degU32(Hy) and degS200 (Hy) mutants. The degU32(Hy) mutation caused a 99% reduction in the expression of a sigD-lacZ translational fusion gene cons...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.16.5177-5180.1994
更新日期:1994-08-01 00:00:00
abstract::Welch, Ann Marie (U. S. Veterans Administration Hospital, Durham, N. C.). Preliminary survey of fungistatic properties of marine algae. J. Bacteriol. 83:97-99. 1962-Homogenized preparations of 35 marine algae were tested for inhibitory activity against 6 pathogenic or opportunistically pathogenic fungi with saturated ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.1.97-99.1962
更新日期:1962-01-01 00:00:00
abstract::Approximately 2 x 10(11) viable Pasteurella tularensis cells per ml, contained in suspensions, were killed by exposure to 10(6) r of gamma-radiation. When injected intraperitoneally into mice, the irradiated suspensions initially contained about 10 ld(50) per ml, and immunized mice against challenge with fully virulen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1968-09-01 00:00:00
abstract::The presence of phospho-beta-glucosidases and beta-glucoside permeases was found in strains of Streptococcus, Bacillus, and Staphylococcus. In streptococci, the phospho-beta-glucosidase activity depends on the antigenic group. The highest activity was found in strains of group D. In group D strains, phospho-beta-gluco...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.99.2.434-440.1969
更新日期:1969-08-01 00:00:00
abstract::The ability to reduce Hg(II) to Hg(0), which is determined by a plasmid-borne gene in Escherichia coli, is conferred by a Hg(II)-inducible activity which is located in the cytoplasm rather than in the periplasmic space of the cell. This Hg(II)-reducing activity can be isolated from the supernatant of a 160,000 x g cen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.119.1.242-249.1974
更新日期:1974-07-01 00:00:00
abstract::Bacterial lysis induced by the expression of the cloned lysis gene of the RNA bacteriophage MS2 in Escherichia coli was shown to be under the same regulatory control mechanisms as penicillin-induced lysis. It was controlled by the stringent response and showed the phenomenon of tolerance when E. coli was grown at pH 5...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.11.5027-5033.1988
更新日期:1988-11-01 00:00:00
abstract::Salmonella typhimurium was found to utilize 2-deoxyribose as a sole carbon and energy source. Cells grown in the presence of deoxyribose contained increased levels of deoxyribose kinase, thymidine phosphorylase, and two forms of deoxyribose-5-phosphate aldolase (DR5P aldolase). One form of DR5P aldolase was induced by...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.2.449-457.1968
更新日期:1968-02-01 00:00:00
abstract:UNLABELLED:Pupylation is a posttranslational modification peculiar to actinobacteria wherein proteins are covalently modified with a small protein called the prokaryotic ubiquitin-like protein (Pup). Like ubiquitination in eukaryotes, this phenomenon has been associated with proteasome-mediated protein degradation in m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00302-15
更新日期:2015-09-01 00:00:00
abstract::The ompB operon of Escherichia coli contains the structural genes for two proteins, OmpR and EnvZ, which control the osmoregulated biosynthesis of the porin proteins OmpF and OmpC. By inserting XbaI octamer linkers into the cloned ompB locus, four distinct frameshift mutants were isolated and subsequently characterize...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.2.578-584.1985
更新日期:1985-11-01 00:00:00
abstract::DNA-binding assays using mobility shift polyacrylamide gel electrophoresis revealed the presence of a protein that specifically bound to a restriction fragment -288 to -191 bp upstream from the transcriptional start point of strR, a regulatory gene for streptomycin biosynthesis in Streptomyces griseus. The binding sit...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.9.2652-2661.1993
更新日期:1993-05-01 00:00:00
abstract::Nickel-deficient (Nic-) mutants of Alcaligenes eutrophus requiring high levels of nickel ions for autotrophic growth with hydrogen were characterized. The Nic- mutants carried defined deletions in the hydrogenase gene cluster of the indigenous pHG megaplasmid. Nickel deficiency correlated with a low level of the nicke...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.3.1340-1345.1989
更新日期:1989-03-01 00:00:00
abstract::The genes governing three of the enzymes of the isoleucine-valine biosynthetic pathway form the operon: operator-ilvA-ilvD-ilvE. The enzymes are: ilvA, l-threonine deaminase; ilvD, dihydroxy acid dehydrase; and ilvE, transaminase B. A nonsense mutation in the ilvD gene (D-ochre) and a nonsense mutation in the ilvE gen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.98.3.1179-1194.1969
更新日期:1969-06-01 00:00:00
abstract::The ski22::Tn917lac insertion mutation in Bacillus subtilis was isolated in a screen for mutations that cause a defect in sporulation but are suppressed by the presence or overexpression of the histidine protein kinase encoded by kinA (spoIIJ). The ski22::Tn917lac insertion mutation was in ald, the gene encoding alani...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.21.6789-6796.1993
更新日期:1993-11-01 00:00:00
abstract::The draft genome sequence (4,398,155 bp, with 65.35% G+C content) of Halobiforma lacisalsi AJ5, an extremely halophilic archaeon isolated from a salt lake, is reported here. This is the first genome report for a species of the Halobiforma genus. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06282-11
更新日期:2011-12-01 00:00:00
abstract::Shewanella oneidensis is a metal reducer that uses the cyclic AMP receptor protein, CRP, to regulate anaerobic respiration. In addition, ArcA(So) is required for anaerobic growth with dimethyl sulfoxide (DMSO) and plays a role in aerobic respiration. The sensor kinase that activates ArcA(So) in S. oneidensis is not kn...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01695-09
更新日期:2010-06-01 00:00:00
abstract::The genes encoding ammonia monooxygenase (amoCAB), hydroxylamine oxidoreductase (hao), and the c-type cytochrome c-554 (hcy) are present in multiple copies in the genome of Nitrosomonas europaea. The upstream regions of the two copies of amoC, the three copies of hao, and one copy of hcy were cloned and sequenced. Pri...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.3.1096-1100.2001
更新日期:2001-02-01 00:00:00
abstract::Five lacY mutants with amber stop codons at known positions were each placed into 12 different suppressor strains. The 60 amino acid substitutions obtained in this manner were tested for growth on lactose-minimal medium plates and for transport of lactose, melibiose, and thiomethylgalactoside. Most of the amino acid s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.16.5436-5441.1992
更新日期:1992-08-01 00:00:00
abstract::Conjugative transfer and replacement of hundreds of kilobases of a bacterial chromosome can occur in vitro, but replacements in nature are either an order of magnitude smaller or involve the movement of mobile genetic elements. We discovered that two lineages of Staphylococcus aureus, including a pandemic methicillin-...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.4.1060-1064.2004
更新日期:2004-02-01 00:00:00
abstract::Mutants of Escherichia coli K-12 constitutive for fatty acid degradation (fadR) showed an increased rate of utilization of exogenous acetate. Acetate transport, oxidation, and incorporation into macromolecules was approximately fivefold greater in fadR mutants than fadR+ strains during growth on succinate as a carbon ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.148.1.83-90.1981
更新日期:1981-10-01 00:00:00
abstract::Aspartate transcarbamylase is synthesized during exponential growth of Bacillus subtilis and is inactivated when the cells enter the stationary phase. This work is a study of the regulation of aspartate transcarbamylase synthesis during growth and the stationary phase. Using specific immunoprecipitation of aspartate t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.135.3.943-951.1978
更新日期:1978-09-01 00:00:00
abstract::Cell walls (LOG walls) were isolated from cultures of Streptococcus faecalis ATCC 9790 in the exponential phase of growth. These walls were either allowed to undergo autolytic dissolution (in the presence or absence of trypsin) or wall autolysis was inactivated with sodium dodecylsulfate (SDS walls). Inactivated walls...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.5.1803-1810.1968
更新日期:1968-11-01 00:00:00
abstract::Citrate metabolism in Leuconostoc mesenteroides subspecies mesenteroides is associated with the generation of a proton motive force by a secondary mechanism (C. Marty-Teysset, C. Posthuma, J. S. Lolkema, P. Schmitt, C. Divies, and W. N. Konings, J. Bacteriol. 178:2178-2185, 1996). The pathway consists of four steps: (...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.21.6209-6215.1996
更新日期:1996-11-01 00:00:00
abstract::Transposon Tn917 mutagenesis of Bacillus subtilis BD99 followed by selection for protonophore resistance led to the isolation of strain MS119, which contained a single Tn917 insertion in an open reading frame whose deduced amino acid sequence was 56.6% identical to that of the Escherichia coli rho gene product. The in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.3.647-654.1993
更新日期:1993-02-01 00:00:00