Abstract:
:Methanohalophilus strain FDF1 synthesizes beta-glutamine, betaine, and N epsilon-acetyl-beta-lysine as osmoprotective agents when the cells are grown in high external concentrations of NaCl. Nuclear magnetic resonance spectroscopic analyses of 13CH3OH-12CO2 label incorporation by the cells provide information on the biosynthetic pathways of these organic osmolytes. The labeling studies indicate that Methanohalophilus strain FDF1 produces glutamate and beta-glutamine via a partial oxidative Krebs pathway. 13C labeling of betaine is consistent with methylation of glycine generated from serine (via serine hydroxymethyltransferase). The labeling pattern for N epsilon-acetyl-beta-lysine is consistent with the synthesis of its precursor alpha-lysine occurring by the diaminopimelate pathway in these cells.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Roberts MF,Lai MC,Gunsalus RPdoi
10.1128/jb.174.20.6688-6693.1992keywords:
subject
Has Abstractpub_date
1992-10-01 00:00:00pages
6688-93issue
20eissn
0021-9193issn
1098-5530journal_volume
174pub_type
杂志文章abstract::Succinate dehydrogenase (EC 1.3.99.1) of Micrococcus luteus was selectively precipitated from Triton X-100-solubilized membranes by using specific antiserum. The precipitated enzyme contained equimolar amounts of four polypeptides with apparent molecular weights of 72,000, 30,000, 17,000, and 15,000. The 72,000 polype...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.3.1493-1501.1983
更新日期:1983-03-01 00:00:00
abstract::Atractyloside and carboxyatractyloside partially inhibited nitrogenase activity (acetylene reduction) by isolated vesicles of Frankia strain EAN1pec. Extracts of disrupted vesicles showed nitrogenase activity that was not affected by the inhibitors. The vesicles accumulated ATP by an atractyloside-sensitive mechanism....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.7.3053-3057.1988
更新日期:1988-07-01 00:00:00
abstract::Shafia, Fred (University of Nebraska, Lincoln), and T. L. Thompson. Isolation and preliminary characterization of bacteriophage phimu-4. J. Bacteriol. 87:999-1002. 1964.-Bacteriophage phimu-4 was isolated from lysogenic Bacillus stearothermophilus NU strain 4, and was propagated in strain 10 of the same species. The p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.5.999-1002.1964
更新日期:1964-05-01 00:00:00
abstract::A cell surface antigen complex from Zwittergent-solubilized Myxococcus xanthus has been purified by immunoaffinity chromatography with monoclonal antibody (MAb) 1604 and by subsequent gel filtration. We propose that the cell surface antigen (CSA) 1604 complex participates in intercellular interactions. The apparent to...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.9.4655-4666.1989
更新日期:1989-09-01 00:00:00
abstract::Certain oral treponemes express a highly proteolytic phenotype and have been associated with periodontal diseases. The periodontal pathogen Treponema denticola produces dentilisin, a serine protease of the subtilisin family. The two-gene operon prcA-prtP is required for expression of active dentilisin (PrtP), a putati...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.23.6860-6869.2003
更新日期:2003-12-01 00:00:00
abstract::The reproduction and morphogenesis of Rickettsiella chironomi, an unusual procaryotic parasite of midge larvae, was studied with electron microscopy. The morphogenic cycle was similar to that found in chlamydia and consisted of four major cell types: (i) medium-sized spherical initial bodies (ca. 1 micrometers in diam...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.2.995-1002.1980
更新日期:1980-08-01 00:00:00
abstract::The Escherichia coli promoter p(BAD), under the control of the AraC protein, drives the expression of mRNA encoding the AraB, AraA, and AraD gene products of the arabinose operon. The binding site of AraC at p(BAD) overlaps the RNA polymerase -35 recognition region by 4 bases, leaving 2 bases of the region not contact...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.18.5076-5081.2000
更新日期:2000-09-01 00:00:00
abstract::The marRAB operon is one of two operons in the mar locus of Escherichia coli that are divergently transcribed from a central regulatory region, marO. The marRAB operon, transcribed from marOII, controls intrinsic resistance or susceptibility to multiple antibiotics and is inducible by structurally unrelated compounds ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.12.3414-3419.1995
更新日期:1995-06-01 00:00:00
abstract::The age-dependent lectin-binding ability of Rhizobium trifolii 0403 capsular polysaccharide (CPS) was examined by following the development of the capsule and its ability to interact with the white clover lectin trifoliin A. Bacteria grown on agar plates for 3, 5, 7, 14, and 21 days were examined by electron microscop...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.159.1.145-152.1984
更新日期:1984-07-01 00:00:00
abstract::Smith, Doyle C. (Kansas State University, Manhattan), V. D. Foltz, and T. H. Lord. Two lytic factors involved in induced synergistic hemolysis in normally nonhemolytic staphylococci. J. Bacteriol. 88:1700-1704. 1964.-Synergistic hemolysis of "normally nonhemolytic" staphylococci results from the combination of two fac...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.6.1700-1704.1964
更新日期:1964-12-01 00:00:00
abstract::Myxococcus xanthus dsp and dif mutants have similar phenotypes in that they are deficient in social motility and fruiting body development. We compared the two loci by genetic mapping, complementation with a cosmid clone, DNA sequencing, and gene disruption and found that 16 of the 18 dsp alleles map to the dif genes....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.5.1462-1465.2002
更新日期:2002-03-01 00:00:00
abstract::Purified L-asparaginase from Serratia marcescens had an apparent-weight average molecular weight of 171,000 to 180,000 as determined by electrophoresis on polyacrylamide gels and by sedimentation equilibrium at low speed in an analytical ultracentrifuge. A subunit molecular weight of 31,500 +/- 1,500 was estimated for...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.2.719-727.1976
更新日期:1976-02-01 00:00:00
abstract::We isolated five new temperature-sensitive alleles of the essential cell division gene ftsZ in Escherichia coli, using P1-mediated, localized mutagenesis. The five resulting single amino acid changes (Gly109-->Ser109 for ftsZ6460, Ala129-->Thr129 for ftsZ972, Val157-->Met157 for ftsZ2066, Pro203-->Leu203 for ftsZ9124,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.1.358-365.2005
更新日期:2005-01-01 00:00:00
abstract::UDP-N-acetylmuramic acid:L-alanine ligase (MurC) catalyzes the addition of the first amino acid to the cytoplasmic precursor of the bacterial cell wall peptidoglycan. The crystal structures of Haemophilus influenzae MurC in complex with its substrate UDP-N-acetylmuramic acid (UNAM) and Mg(2+) and of a fully assembled ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.14.4152-4162.2003
更新日期:2003-07-01 00:00:00
abstract::We have developed a general method for determining whether a gene of Escherichia coli is essential for viability. The method requires cloned DNA spanning the gene in question and a reasonably detailed genetic and physical map of the cloned segment. Using this information, one constructs a deletion of the target gene i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.2.636-643.1984
更新日期:1984-05-01 00:00:00
abstract::This article contains an analysis of a version of the well-known inhibitor-dilution model for the control of initiation of chromosome replication in bacteria. According to this model, an unstable inhibitor interacts with an initiation primer in a hit-and-destroy fashion to prevent successful initiation; both constitue...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.11.5231-5240.1987
更新日期:1987-11-01 00:00:00
abstract::The cheF gene, which is involved in chemotaxis in Bacillus subtilis, has been cloned, expressed, and sequenced. This gene is contained in a 0.7-kilobase PstI DNA fragment that was isolated from a lambda Charon 4A B. subtilis chromosomal DNA library. This fragment was subcloned into the expression vector pSI-1 and show...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.3.1631-1637.1989
更新日期:1989-03-01 00:00:00
abstract::After the Bacillus subtilis nucleoid was dissected with restriction endonucleases, a specific DNA sequence from the purA region was isolated in a particulate form that probably originated from the cell membrane. Precise definition of the binding region within this sequence was achieved by a novel procedure based on a ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.1.38-43.1986
更新日期:1986-04-01 00:00:00
abstract::The Gram-positive bacterium Streptococcus pyogenes (also called group A Streptococcus [GAS]), is found strictly in humans and is capable of causing a wide variety of infections. Here we demonstrate that serine catabolism in GAS is controlled by the transcriptional regulator Spy49_0126c. We have designated this regulat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01516-10
更新日期:2011-04-01 00:00:00
abstract::Escherichia coli WP2 bacteria with an ochre amino acid auxotrophy show no evidence of growth during the first few days after plating at densities above 10(8) on plates lacking the required amino acid. They lose viability for some days, and then a subpopulation recovers and there is cell turnover. At very low plating d...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.11.2906-2910.1998
更新日期:1998-06-01 00:00:00
abstract::The phage 21 holin, S(21), forms small membrane holes that depolarize the membrane and is designated as a pinholin, as opposed to large-hole-forming holins, like S(lambda). Pinholins require secreted SAR endolysins, a pairing that may represent an intermediate in the evolution of canonical holin-endolysin systems. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00847-07
更新日期:2007-12-01 00:00:00
abstract::The sog gene of the IncI alpha group plasmid ColIb is known to encode a DNA primase that can substitute for defective host primase in dnaG mutants of Escherichia coli during discontinuous DNA replication. The biological significance of this enzyme was investigated by using sog mutants, constructed from a derivative of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1982-12-01 00:00:00
abstract::Bacillus licheniformis strain 749/C (constitutive for penicillinase formation) and uninduced cells of strain 749 (penicillinase-inducible) were examined after freezeetching. In the early stationary phase, strain 749/C organisms had clusters of vesicles (30 to 40 nm in diameter) on the outer surface of the plasma membr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.2.1002-1009.1969
更新日期:1969-11-01 00:00:00
abstract::Halobacterium halobium spontaneously reverses its swimming direction with a frequency of about 0.1 s-1. The self-sustained periodicity can be entrained by rhythmic light stimuli within a limited range between 0.13 and 0.3 s-1. Increase of temperature increases the reversal frequency and shortens the time to reach maxi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.2.689-692.1986
更新日期:1986-05-01 00:00:00
abstract::The Arctic plant growth-promoting rhizobacterium Pseudomonas putida GR12-2 secretes an antifreeze protein (AFP) that promotes survival at subzero temperatures. The AFP is unusual in that it also exhibits a low level of ice nucleation activity. A DNA fragment with an open reading frame encoding 473 amino acids was clon...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.17.5661-5671.2004
更新日期:2004-09-01 00:00:00
abstract::The dibenzofuran-degrading bacterial strain DPO360 represents a new species of the genus Terrabacter together with the previously described dibenzofuran-mineralizing bacterial strain DPO1361 (K.-H. Engesser, V. Strubel, K. Christoglou, P. Fischer, and H. G. Rast, FEMS Microbiol. Lett. 65:205-210, 1989; V. Strubel, Ph....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.1.53-62.1997
更新日期:1997-01-01 00:00:00
abstract::Analysis of deoxyribonucleic acid (DNA) from four species of Clostridium, including two thermophiles, a mesophile, and a psychrophile, revealed no obvious relationship between growth temperature and DNA base composition. The melting temperatures (T(m)) of the DNA from the four species varied no more among the thermoph...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.1.252-262.1973
更新日期:1973-01-01 00:00:00
abstract::The human pathogen Eikenella corrodens expresses type IV pili and exhibits a phase variation involving the irreversible transition from piliated to nonpiliated variants. On solid medium, piliated variants form small (S-phase), corroding colonies whereas nonpiliated variants form large (L-phase), noncorroding colonies....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.1.55-62.2001
更新日期:2001-01-01 00:00:00
abstract::Four mutants specifically deficient in the activity of isocitrate lyase were independently isolated in the alkane yeast Saccharomycopsis lipolytica. Genetic analysis by means of protoplast fusion and mitotic haploidization revealed that the mutations were recessive and non-complementary at a single genetic locus, icl....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.157.3.899-908.1984
更新日期:1984-03-01 00:00:00
abstract::Utilization of heme as an iron source by Bradyrhizobium japonicum involves induction of the outer membrane heme receptor gene hmuR and other genes within the heme utilization locus. Here, we discovered the hmuP gene located upstream of hmuR and transcribed divergently from it along with hmuTUV. hmuP encodes a small pr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00071-12
更新日期:2012-06-01 00:00:00