Overproduction of DnaE protein (alpha subunit of DNA polymerase III) restores viability in a conditionally inviable Escherichia coli strain deficient in DNA polymerase I.

Abstract:

:A polA12 recA718 double mutant of Escherichia coli, in which DNA polymerase I is temperature sensitive, was unable to maintain normal DNA synthesis or to form colonies on rich media at 42 degrees C. Overproduction of DnaE protein, the polymerizing alpha subunit of DNA polymerase III, restored bacterial DNA replication and cell viability, as well as the PolI-dependent replication of the plasmid carrying dnaE.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Witkin EM,Roegner-Maniscalco V

doi

10.1128/jb.174.12.4166-4168.1992

keywords:

subject

Has Abstract

pub_date

1992-06-01 00:00:00

pages

4166-8

issue

12

eissn

0021-9193

issn

1098-5530

journal_volume

174

pub_type

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