Analysis of the dynamics of a Bacillus subtilis spore germination protein complex during spore germination and outgrowth.

abstract：:In this study, the H303A mutant form of the cbb(3) oxidase (H303A oxidase), which has the H303A mutation in its catalytic subunit (CcoN), was purified from Rhodobacter sphaeroides. The H303A oxidase showed the same catalytic activity as did the wild-type form of the oxidase (WT oxidase). The heme contents of the mutan...

journal_title：Journal of bacteriology

authors： Kim YJ,Ko IJ,Lee JM,Kang HY,Kim YM,Kaplan S,Oh JI

更新日期：2007-08-01 00:00:00

abstract：UNLABELLED:Escherichia coli RtcB is a founding member of a family of manganese-dependent RNA repair enzymes that join RNA 2′,3′-cyclic phosphate (RNA>p) or RNA 3′-phosphate (RNAp) ends to 5′-OH RNA (HORNA) ends in a multistep pathway whereby RtcB (i) hydrolyzes RNA>p to RNAp, (ii) transfers GMP from GTP to RNAp to form...

journal_title：Journal of bacteriology

authors： Maughan WP,Shuman S

更新日期：2016-03-31 00:00:00

abstract：:The first three enzymes of the pentachlorophenol (PCP) degradation pathway in Sphingobium chlorophenolicum (formerly Sphingomonas chlorophenolica) ATCC 39723 have been characterized, and the corresponding genes, pcpA, pcpB, and pcpC, have been individually cloned and sequenced. To search for new genes involved in PCP ...

journal_title：Journal of bacteriology

authors： Cai M,Xun L

更新日期：2002-09-01 00:00:00

abstract：:Two different mutations (pabA and pabB) affecting 4-aminobenzoate biosynthesis were obtained in strains of Escherichia coli lacking chorismate mutase and anthranilate synthetase activity, thus allowing study of the pathway of biosynthesis of 4-aminobenzoate by use of cell extracts of strains carrying the pab mutations...

journal_title：Journal of bacteriology

authors： Huang M,Gibson F

更新日期：1970-06-01 00:00:00

abstract：:DNA sequence analysis of a region of the Streptomyces sp. strain C5 daunomycin biosynthesis gene cluster, located between the daunomycin polyketide biosynthesis gene cluster and a dnrI (transcriptional activator) homolog, revealed the presence of a gene encoding a P-450-like enzyme with a deduced Mr of 46,096. Express...

journal_title：Journal of bacteriology

authors： Dickens ML,Strohl WR

更新日期：1996-06-01 00:00:00

abstract：:Ferric reductase activity was examined in Azotobacter vinelandii and was found to be located in the cytoplasm. The specific activities of soluble cell extracts were not affected by the iron concentration of the growth medium; however, activity was inhibited by the presence of Zn2+ during cell growth and also by the ad...

journal_title：Journal of bacteriology

authors： Huyer M,Page WJ

更新日期：1989-07-01 00:00:00

abstract：:Some strains of Streptomyces produce sialidases. Two sialidases were purified over 1,000-fold from a culture filtrate of two Streptomyces species. They had the same properties in molecular weight, behavior to ions and other reagents, and substrate specificity. They showed very small differences in kinetic properties, ...

journal_title：Journal of bacteriology

authors： Kunimoto S,Aoyagi T,Takeuchi T,Umezawa H

更新日期：1974-08-01 00:00:00

abstract：:MurG is a peripheral membrane protein that is one of the key enzymes in peptidoglycan biosynthesis. The crystal structure of Escherichia coli MurG (S. Ha, D. Walker, Y. Shi, and S. Walker, Protein Sci. 9:1045-1052, 2000) contains a hydrophobic patch surrounded by basic residues that may represent a membrane associatio...

journal_title：Journal of bacteriology

authors： van den Brink-van der Laan E,Boots JW,Spelbrink RE,Kool GM,Breukink E,Killian JA,de Kruijff B

更新日期：2003-07-01 00:00:00

abstract：:It has been reported that the accumulation of K+ by Streptococcus faecalis is mediated by a transport system which required both ATP and the proton motive force (Bakker and Harold, J. Biol. Chem. 255:433-440, 1980). My results indicate that S. faecalis has a second transport system for K+. The features of this system ...

journal_title：Journal of bacteriology

authors： Kobayashi H

更新日期：1982-05-01 00:00:00

abstract：:We examined in detail conditions necessary for making reproducible and for maximizing the amount of deoxyribonucleic acid obtained from a sex factor-containing cell as covalent circles. The results argue that under optimal conditions covalent circles are neither created nor lost during the isolation procedure. The cau...

journal_title：Journal of bacteriology

authors： Freifelder D,Folkmanis A,Kirschner I

更新日期：1971-03-01 00:00:00

abstract：:We report a phenomenon similar to catabolite repression in Rhizobium meliloti. Succinate, which allows the highest observed rate of growth of R. meliloti, caused an immediate reduction of beta-galactosidase activity when added to cells growing in lactose. A Lac- mutant was unaltered in nodulation and nitrogen fixation...

journal_title：Journal of bacteriology

authors： Ucker DS,Signer ER

更新日期：1978-12-01 00:00:00

abstract：:The Bacillus subtilis skin element confers resistance to arsenate and arsenite. The ars operon in the skin element contains four genes in the order arsR, ORF2, arsB, and arsC. Three of these genes are homologous to the arsR, arsB, and arsC genes from the staphylococcal plasmid pI258, while no homologs of ORF2 have bee...

journal_title：Journal of bacteriology

authors： Sato T,Kobayashi Y

更新日期：1998-04-01 00:00:00

abstract：:Membrane vesicles isolated from competent cultures of Bacillus subtilis 168 bound up to 20 mug of double-stranded deoxyribonucleic acid (DNA) per mg of membrane protein in the presence of ethylenediaminetetraacetate. The formation of the DNA-membrane complex was time, temperature, and pH dependent. Eighty per cent of ...

journal_title：Journal of bacteriology

authors： Joenje H,Konings WN,Venema G

更新日期：1974-09-01 00:00:00

abstract：:The first alkaline phosphatase (APase) structural gene mutant of Bacillus subtilis 168 was constructed by using a clone identified by hybridization to a synthetic degenerative oligonucleotide. The design of the probe was based on the first 29 amino acids of the sequenced mature APase III protein, which had been isolat...

journal_title：Journal of bacteriology

authors： Bookstein C,Edwards CW,Kapp NV,Hulett FM

更新日期：1990-07-01 00:00:00

abstract：:Entry into sporulation in Bacillus subtilis is characterized by the formation of a polar septum, which asymmetrically divides the developing cell into forespore (the smaller cell) and mother cell compartments, and by migration of replication origin regions to extreme opposite poles of the cell. Here we show that polar...

journal_title：Journal of bacteriology

authors： Graumann PL,Losick R

更新日期：2001-07-01 00:00:00

abstract：:DNA sequence analysis of the modABCD operon of Escherichia coli revealed the presence of four open reading frames. The first gene, modA, codes for a 257-amino-acid periplasmic binding protein enunciated by the presence of a signal peptide-like sequence. The second gene (modB) encodes a 229-amino-acid protein with a po...

journal_title：Journal of bacteriology

authors： Maupin-Furlow JA,Rosentel JK,Lee JH,Deppenmeier U,Gunsalus RP,Shanmugam KT

更新日期：1995-09-01 00:00:00

abstract：:The regulatory proteins OmpR and EnvZ are both required to activate expression of the genes for the major outer membrane porin proteins, OmpF and OmpC, of Escherichia coli K-12. Here we show that OmpR, under certain conditions, could activate porin expression in the complete absence of EnvZ. In addition, the pleiotrop...

journal_title：Journal of bacteriology

authors： Slauch JM,Garrett S,Jackson DE,Silhavy TJ

更新日期：1988-01-01 00:00:00

abstract：:Strains of Escherichia coli K12 have been prepared that carry the ruv mutation in combination with lon or uvrA mutant alleles. The ruv minus uvrA minus double mutant is more sensitive to ultraviolet light than the urvA minus single mutant, whereas the strain with ruv and ion mutations shows the same sensitivity to ult...

journal_title：Journal of bacteriology

authors： Iyehara H,Otsuji N

更新日期：1975-02-01 00:00:00

abstract：:The multicellular developmental cycle of Myxococcus xanthus requires large-scale changes in gene transcription, and recent findings indicate that NtrC-like activators play a prominent role in regulating these changes. In this study, we made insertions in 28 uncharacterized ntrC-like activator (nla) genes and found tha...

journal_title：Journal of bacteriology

authors： Caberoy NB,Welch RD,Jakobsen JS,Slater SC,Garza AG

更新日期：2003-10-01 00:00:00

abstract：:The sdiA gene codes for a protein that regulates expression of the ftsQAZ cluster of essential cell division genes of Escherichia coli. SdiA up-regulates the ftsQ2p promoter that initiates transcription into the ftsQAZ cluster. In this paper, we report that expression of sdiA is itself regulated by a factor that is re...

journal_title：Journal of bacteriology

authors： García-Lara J,Shang LH,Rothfield LI

更新日期：1996-05-01 00:00:00

abstract：:From citrate-utilizing (Cit+) Escherichia coli strain C53 of bovine origin, strains C53A and C53B were obtained. Upon mating with recA+ but not with recA mutant recipients of K-12, C53A produced chromosomal recombinants at quite high frequencies, leading to the following conclusions: (i) C53A is an Hfr strain; (ii) th...

journal_title：Journal of bacteriology

authors： Ishiguro N,Oka C,Asagi M,Sato G,Yoshikawa M

更新日期：1980-08-01 00:00:00

abstract：:The conversion of Pseudomonas aeruginosa PAO to the mucoid phenotype has been reported for a chronic pulmonary infection model in rats (D. E. Woods, P. A. Sokol, L. E. Bryan, D. G. Storey, S. J. Mattingly, H. J. Vogel, and H. Ceri, J. Infect. Dis. 163:143-149, 1991). This conversion was associated with a genetic rearr...

journal_title：Journal of bacteriology

authors： Sokol PA,Luan MZ,Storey DG,Thirukkumaran P

更新日期：1994-02-01 00:00:00

abstract：:Filaments of the heterocyst-forming cyanobacterium Anabaena variabilis reproduced 35SO4(2)-, incorporating 35S into cysteine, methionine, glutathione, sulfolipid, and several unidentified metabolites. The majority of the incorporated label accumulated in reduced glutathione. Heterocysts isolated from labeled filaments...

journal_title：Journal of bacteriology

authors： Giddings TH Jr,Wolk CP,Shomer-Ilan A

更新日期：1981-06-01 00:00:00

abstract：:Several authors have suggested that the SOS-associated (sfiA-dependent) system of division inhibition, normally induced by perturbations of DNA replication, also regulates steady-state (unperturbed) cell division. The present work shows that mean cell mass is identical in sfiA+ and sfiA mutant cultures during steady-s...

journal_title：Journal of bacteriology

authors： Huisman O,Jacques M,D'ari R,Caro L

更新日期：1983-02-01 00:00:00

abstract：:Welker, N. E. (Western Reserve University, Cleveland, Ohio), and L. Leon Campbell. Preferential synthesis of alpha-amylase by Bacillus stearothermophilus in the presence of 5-methyl-tryptophan. J. Bacteriol. 87:828-831. 1964.-Washed-cell suspensions of Bacillus stearothermophilus induced with pure maltose preferential...

journal_title：Journal of bacteriology

authors： WELKER NE,CAMPBELL LL

更新日期：1964-04-01 00:00:00

abstract：:Sneathia amnii is a poorly characterized emerging pathogen that has been implicated in amnionitis and urethritis. We found that S. amnii damages fetal membranes, and we identified and purified a cytotoxic exotoxin that lyses human red blood cells and damages cells from fetal membranes. The gene appears to be cotranscr...

journal_title：Journal of bacteriology

authors： Gentile GL,Rupert AS,Carrasco LI,Garcia EM,Kumar NG,Walsh SW,Jefferson KK

更新日期：2020-06-09 00:00:00

abstract：:Fructose, galactose, L-arabinose, gluconate, and several organic acids support rapid growth and N2 fixation of Azospirillum brasiliense ATCC 29145 (strain Sp7) as a sole source of carbon and energy. Growth of Azospirillum lipoferum ATCC 29707 (strain Sp59b) is also supported by glucose, mannose, mannitol, and alpha-ke...

journal_title：Journal of bacteriology

authors： Martinez-Drets G,Del Gallo M,Burpee C,Burris RH

更新日期：1984-07-01 00:00:00

abstract：:In Escherichia coli K-12, the rcsA and rcsB gene products are positive regulators in expression of the slime polysaccharide colanic acid. We have previously demonstrated the presence of rcsA sequences in E. coli K1 and K5, strains with group II capsular K antigens, and shown that introduction of multicopy rcsA into th...

journal_title：Journal of bacteriology

authors： Keenleyside WJ,Bronner D,Jann K,Jann B,Whitfield C

更新日期：1993-10-01 00:00:00

abstract：:Three polynucleotide phosphorylase mutations, isolated in heavily mutagenized Escherichia coli strains Q7, Q13, and Q27, were characterized after their transfer by P1 transduction to nearly isogenic strains which lack ribonuclease I. Each strain has a different altered form of polynucleotide phosphorylase. One enzyme ...

journal_title：Journal of bacteriology

authors： Reiner AM

更新日期：1969-03-01 00:00:00

abstract：:Fluctuations in osmolarity are one of the most prevalent stresses to which bacteria must adapt, both hypo- and hyperosmotic conditions. Most bacteria cope with high osmolarity by accumulating compatible solutes (osmolytes) in the cytoplasm to maintain the turgor pressure of the cell. Vibrio parahaemolyticus, a halophi...

journal_title：Journal of bacteriology

authors： Gregory GJ,Dutta A,Parashar V,Boyd EF

更新日期：2020-11-19 00:00:00