Coexpression of colanic acid and serotype-specific capsular polysaccharides in Escherichia coli strains with group II K antigens.

Abstract:

:In Escherichia coli K-12, the rcsA and rcsB gene products are positive regulators in expression of the slime polysaccharide colanic acid. We have previously demonstrated the presence of rcsA sequences in E. coli K1 and K5, strains with group II capsular K antigens, and shown that introduction of multicopy rcsA into these strains results in the expression of colanic acid. We report here the presence of rcsB sequences in E. coli K1 and K5 and demonstrate that RcsB also plays a role in the biosynthesis of colanic acid in strains with group II K antigens. In E. coli K1 and K5 grown at 37 degrees C, multicopy rcsB and the resulting induction of colanic acid synthesis had no significant effect on synthesis of the group II K antigens. K-antigen-specific sugar transferase activities were not significantly different in the presence or absence of multicopy rcsB, and introduction of a cps mutation to eliminate colanic acid biosynthesis in a K1-derivative strain did not influence the activity of the polysialyltransferase enzyme responsible for synthesis of the K1 polymer. Furthermore, immunoelectron microscopy showed no detectable difference in the size or distribution of the group II K-antigen capsular layer in cells which produced colanic acid. Colanic acid expression therefore does not appear to significantly affect synthesis of the group II K-antigen capsule and, unlike for group I K antigens, expression of group II K antigens is not positively regulated by the rcs system.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Keenleyside WJ,Bronner D,Jann K,Jann B,Whitfield C

doi

10.1128/jb.175.20.6725-6730.1993

subject

Has Abstract

pub_date

1993-10-01 00:00:00

pages

6725-30

issue

20

eissn

0021-9193

issn

1098-5530

journal_volume

175

pub_type

杂志文章
  • Role of the Eikenella corrodens pilA locus in pilus function and phase variation.

    abstract::The human pathogen Eikenella corrodens expresses type IV pili and exhibits a phase variation involving the irreversible transition from piliated to nonpiliated variants. On solid medium, piliated variants form small (S-phase), corroding colonies whereas nonpiliated variants form large (L-phase), noncorroding colonies....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.1.55-62.2001

    authors: Villar MT,Hirschberg RL,Schaefer MR

    更新日期:2001-01-01 00:00:00

  • Protein mobility in the cytoplasm of Escherichia coli.

    abstract::The rate of protein diffusion in bacterial cytoplasm may constrain a variety of cellular functions and limit the rates of many biochemical reactions in vivo. In this paper, we report noninvasive measurements of the apparent diffusion coefficient of green fluorescent protein (GFP) in the cytoplasm of Escherichia coli. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.1.197-203.1999

    authors: Elowitz MB,Surette MG,Wolf PE,Stock JB,Leibler S

    更新日期:1999-01-01 00:00:00

  • Gap-filling repair synthesis induced by ultraviolet light in a Bacillus subtilis Uvr- mutant.

    abstract::Deoxyribonucleic acid repair synthesis was studied in one wild-type and two mutant strains of Bacillus subtilis that are defective in excision of pyrimidine dimers. The cells were irradiated with ultraviolet light, and 6-(p-hydroxyphenyl-azo)-uracil was used to block replicative synthesis, allowing only repair synthes...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.139.1.239-246.1979

    authors: Hadden CT

    更新日期:1979-07-01 00:00:00

  • Plasmid deoxyribonucleic acid replication in Streptomyces griseus.

    abstract::A series of electron micrographs showing the presence of different molecular forms representing various replication stages of plasmid deoxyribonucleic acid from Streptomyces griseus was obtained. Based upon an analysis of these electron micrographs, a tentative model for plasmid deoxyribonucleic acid replication in S....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.146.1.412-414.1981

    authors: Xue Y,Zhuang Z,Zhu Y,Xu Y,Dong K

    更新日期:1981-04-01 00:00:00

  • Importance of cis determinants and nitrogenase activity in regulated stability of the Klebsiella pneumoniae nitrogenase structural gene mRNA.

    abstract::The Klebsiella pneumoniae nitrogen fixation (nif) mRNAs are unusually stable, with half-lives of 20 to 30 min under conditions favorable to nitrogen fixation (limiting nitrogen, anaerobiosis, temperatures of 30 degrees C). Addition of O2 or fixed nitrogen or temperature increases to 37 degrees C or more result in the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.12.3751-3760.1999

    authors: Simon HM,Gosink MM,Roberts GP

    更新日期:1999-06-01 00:00:00

  • Effect of alanine, leucine and fructose on lysyl-transfer ribonucleic acid ligase activity in a mutant of Escherichia coli K-12.

    abstract::A mutant of Escherichia coli K-12 was examined which has growth medium-dependent lysyl-transfer ribonucleic acid (tRNA) ligase activity. In minimal medium or 0.5% yeast extract, the activity of the enzyme in the mutant strain was 5 to 10% of wild type. However, when the mutant was grown in a highly enriched medium, su...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.1.167-177.1973

    authors: Hirshfield IN,Bukald NE

    更新日期:1973-01-01 00:00:00

  • Cloning and characterization of the gene for a protein thiol-disulfide oxidoreductase in Bacillus brevis.

    abstract::The gene (bdb) for protein thiol-disulfide oxidoreductase cloned from Bacillus brevis was found to encode a polypeptide consisting of 117 amino acid residues with a signal peptide of 27 residues. Bdb contains a well-conserved motif, Cys-X-X-Cys, which functions as the active center of disulfide oxidoreductases such as...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.3.745-749.1995

    authors: Ishihara T,Tomita H,Hasegawa Y,Tsukagoshi N,Yamagata H,Udaka S

    更新日期:1995-02-01 00:00:00

  • Nucleotide sequence of the McrB region of Escherichia coli K-12 and evidence for two independent translational initiation sites at the mcrB locus.

    abstract::The McrB restriction system of Escherichia coli K-12 is responsible for the biological inactivation of foreign DNA that contains 5-methylcytosine residues (E. A. Raleigh and G. Wilson, Proc. Natl. Acad. Sci. USA 83:9070-9074, 1986). Within the McrB region of the chromosome is the mcrB gene, which encodes a protein of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.4.1974-1981.1989

    authors: Ross TK,Achberger EC,Braymer HD

    更新日期:1989-04-01 00:00:00

  • Protein difference between competent and noncompetent cultures of a group H Streptococcus.

    abstract::Acidified phenol extracts prepared from competent cultures of a group H Streptococcus strain Wicky made competent with competence factor derived from cultures of another group H Streptococcus, strain Challis, showed a difference in polyacrylamide-gel protein patterns when compared to extracts prepared from noncompeten...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.1.360-362.1970

    authors: Ranhand JM,Theodore TS,Cole RM

    更新日期:1970-10-01 00:00:00

  • Adenylate energy charge in Saccharomyces cerevisiae during starvation.

    abstract::Bakers' yeast cells, Saccharomyces cerevisiae, if grown aerobically on ethanol or if grown aerobically on glucose and allowed to pass into stationary phase, with utilization of accumulated ethanol, maintain a normal value (0.8 to 0.9) of the adenylate energy charge during prolonged starvation. In contrast, cells grown...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.121.3.975-982.1975

    authors: Ball WJ Jr,Atkinson DE

    更新日期:1975-03-01 00:00:00

  • 2-deoxyribose gene-enzyme complex in Salmonella typhimurium. I. Isolation and enzymatic characterization of 2-deoxyribose-negative mutants.

    abstract::Salmonella typhimurium was found to utilize 2-deoxyribose as a sole carbon and energy source. Cells grown in the presence of deoxyribose contained increased levels of deoxyribose kinase, thymidine phosphorylase, and two forms of deoxyribose-5-phosphate aldolase (DR5P aldolase). One form of DR5P aldolase was induced by...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.2.449-457.1968

    authors: Hoffee PA

    更新日期:1968-02-01 00:00:00

  • Characterization of Tn5 mutants deficient in dissimilatory nitrite reduction in Pseudomonas sp. strain G-179, which contains a copper nitrite reductase.

    abstract::Tn5 was used to generate mutants that were deficient in the dissimilatory reduction of nitrite for Pseudomonas sp. strain G-179, which contains a copper nitrite reductase. Three types of mutants were isolated. The first type showed a lack of growth on nitrate, nitrite, and nitrous oxide. The second type grew on nitrat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.20.6653-6658.1992

    authors: Ye RW,Averill BA,Tiedje JM

    更新日期:1992-10-01 00:00:00

  • Constricted flux through the branched-chain amino acid biosynthetic enzyme acetolactate synthase triggers elevated expression of genes regulated by rpoS and internal acidification.

    abstract::The first common enzyme of isoleucine and valine biosynthesis, acetolactate synthase (ALS), is specifically inhibited by the herbicide sulfometuron methyl (SM). To further understand the physiological consequences of flux alterations at this point in metabolism, Escherichia coli genes whose expression was induced by p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.4.785-792.1998

    authors: Van Dyk TK,Ayers BL,Morgan RW,Larossa RA

    更新日期:1998-02-01 00:00:00

  • Pneumolysin localizes to the cell wall of Streptococcus pneumoniae.

    abstract::Streptococcus pneumoniae is the causative agent of multiple diseases, including otitis media, pneumonia, bacteremia, and meningitis. Pneumolysin (Ply), a member of the cholesterol-dependent cytolytic pore-forming toxins, is produced by virtually all clinical isolates of S. pneumoniae, and strains in which the Ply gene...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01489-08

    authors: Price KE,Camilli A

    更新日期:2009-04-01 00:00:00

  • Analysis of the activity and regulon of the two-component regulatory system composed by Cjj81176_1484 and Cjj81176_1483 of Campylobacter jejuni.

    abstract:UNLABELLED:Campylobacter jejuni is a leading cause of bacterial diarrheal disease and a frequent commensal of the intestinal tract in poultry and other animals. For optimal growth and colonization of hosts, C. jejuni employs two-component regulatory systems (TCSs) to monitor environmental conditions and promote proper ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02564-14

    authors: Luethy PM,Huynh S,Parker CT,Hendrixson DR

    更新日期:2015-05-01 00:00:00

  • The periplasmic protein MppA requires an additional mutated locus to repress marA expression in Escherichia coli.

    abstract::Escherichia coli strain TP985, which has an insertional mutation in the gene for the periplasmic murein tripeptide binding protein MppA, was previously reported to overproduce MarA and exhibit a multiple-antibiotic resistance (Mar) phenotype (H. Li and J. T. Park, J. Bacteriol. 181:4842-4847, 1999). We found that TP98...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.4.1465-1469.2003

    authors: Bina X,Perreten V,Levy SB

    更新日期:2003-02-01 00:00:00

  • Prolonged stationary-phase incubation selects for lrp mutations in Escherichia coli K-12.

    abstract::Evolution by natural selection occurs in cultures of Escherichia coli maintained under carbon starvation stress. Mutants of increased fitness express a growth advantage in stationary phase (GASP) phenotype, enabling them to grow and displace the parent as the majority population. The first GASP mutation was identified...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.15.4361-4365.2000

    authors: Zinser ER,Kolter R

    更新日期:2000-08-01 00:00:00

  • Topological model of the Rhodobacter capsulatus light-harvesting complex I assembly protein LhaA (previously known as ORF1696).

    abstract::A theoretical topology of the Rhodobacter capsulatus membrane protein LhaA was formulated and evaluated by gene fusion experiments. The apparent topological locations of fusion enzymes were compared with the theoretically derived structure, and a model of LhaA is suggested that consists of 12 transmembrane segments, w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.17.4742-4745.1998

    authors: Young CS,Beatty JT

    更新日期:1998-09-01 00:00:00

  • Molecular cloning of TOL genes xylB and xylE in Escherichia coli.

    abstract::The xylB and xylE genes in the TOL plasmid of Pseudomonas putida mt-2, which code for benzyl alcohol dehydrogenase and catechol 2,3-oxygenase, respectively, were cloned onto plasmid pBR322 in Escherichia coli for detailed mapping. The xylB gene was mapped in a 2.9-kilobase region within the BamHI BC fragment of pTN2, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.145.3.1137-1143.1981

    authors: Inouye S,Nakazawa A,Nakazawa T

    更新日期:1981-03-01 00:00:00

  • Separation and characterization of six (1 leads to 3)-beta-glucanases from Saccharomyces cerevisiae.

    abstract::Using a system of chromatography through columns of DEAE-Bio-Gel, HTP-Bio-Gel, and CM-Bio-Gel, we isolated and characterized six different (1 leads to 3)-beta-glucanases from cell wall autolysates and cell extracts of Saccharomyces cerevisiae haploid strain 2180B. These enzymes were designated glucanases I, II, IIIA, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.156.3.1204-1213.1983

    authors: Hien NH,Fleet GH

    更新日期:1983-12-01 00:00:00

  • Transformation analysis of three linkage groups in Staphylococcus aureus.

    abstract::While studying a set of multiply marked mutants of Staphylococcus aureus strain 8325 by transformation, several instances of apparent genetic linkage were encountered. After showing that these linked transformations were readily inactivated by shearing of the deoxyribonucleic acid (DNA) but were resistant to dilution ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.124.1.201-211.1975

    authors: Pattee PA,Neveln DS

    更新日期:1975-10-01 00:00:00

  • Transcriptional organization of the czc heavy-metal homeostasis determinant from Alcaligenes eutrophus.

    abstract::The Czc system of Alcaligenes eutrophus mediates resistance to cobalt, zinc, and cadmium through ion efflux catalyzed by the CzcCB2A cation-proton antiporter. DNA sequencing of the region upstream of the czcNICBADRS determinant located on megaplasmid pMOL30 revealed the 5' end of czcN and a gene for a MgtC-like protei...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.8.2385-2393.1999

    authors: Grosse C,Grass G,Anton A,Franke S,Santos AN,Lawley B,Brown NL,Nies DH

    更新日期:1999-04-01 00:00:00

  • Growth phase-dependent transcription of the genes that encode the two methyl coenzyme M reductase isoenzymes and N5-methyltetrahydromethanopterin:coenzyme M methyltransferase in Methanobacterium thermoautotrophicum delta H.

    abstract::The genes encoding the two isoenzymes of methyl coenzyme M reductase (MRI and MRII) in Methanobacterium thermoautotrophicum delta H have been cloned and sequenced. The MRI-encoding mcr operon (mcrBDCGA) has been located immediately upstream from the mtr operon (mtrEDCBA) that encodes N5-methyltetrahydromethanopterin:c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.20.6384-6391.1994

    authors: Pihl TD,Sharma S,Reeve JN

    更新日期:1994-10-01 00:00:00

  • Origin and fate of the 3' ends of single-stranded DNA generated by conjugal transfer of plasmid R1162.

    abstract::During conjugation, a single strand of DNA is cleaved at the origin of transfer (oriT) by the plasmid-encoded relaxase. This strand is then unwound from its complement and transferred in the 5'-to-3' direction, with the 3' end likely extended by rolling-circle replication. The resulting, newly synthesized oriT must th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00818-12

    authors: Becker EC,Meyer R

    更新日期:2012-10-01 00:00:00

  • Mutational analysis of transmembrane regions 3 and 4 of SecY, a central component of protein translocase.

    abstract::The SecYEG heterotrimeric membrane protein complex functions as a channel for protein translocation across the Escherichia coli cytoplasmic membrane. SecY is the central subunit of the SecYEG complex and contains 10 transmembrane segments (TM1 to TM10). Previous mutation studies suggested that TM3 and TM4 are particul...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.12.3960-3969.2004

    authors: Mori H,Shimokawa N,Satoh Y,Ito K

    更新日期:2004-06-01 00:00:00

  • Involvement of an activation protein in the methanol:2-mercaptoethanesulfonic acid methyltransferase reaction in Methanosarcina barkeri.

    abstract::Methanol:5-hydroxybenzimidazolylcobamide methyltransferase (MT1) is the first of two enzymes required for transfer of the methyl group of methanol to 2-mercaptoethanesulfonic acid in Methanosarcina barkeri. MT1 binds the methyl group of methanol to its corrinoid prosthetic group only when the central cobalt atom of th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.5.1278-1283.1993

    authors: Daas PJ,Gerrits KA,Keltjens JT,van der Drift C,Vogels GD

    更新日期:1993-03-01 00:00:00

  • Structural analysis of the Leptospiraceae and Borrelia burgdorferi by high-voltage electron microscopy.

    abstract::Spirochetes are an evolutionary and structurally unique group of bacteria. Outermost is a membrane sheath (OS), and within this sheath are the protoplasmic cell cylinder (PC) and periplasmic flagella (PFs). The PFs are attached at each end of the PC and, depending on the species, may or may not overlap in the center o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.22.6539-6545.1996

    authors: Goldstein SF,Buttle KF,Charon NW

    更新日期:1996-11-01 00:00:00

  • Isolation of the lipid intermediate in peptidoglycan biosynthesis from Escherichia coli.

    abstract::The lipid intermediate in peptidoglycan biosynthesis was isolated from Escherichia coli strain W and characterized as C(55)-isoprenyl-pyrophosphoryl N-acetylmuramyl(-pentapeptide)-N-acetyl-glucosamine. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.3.1306-1309.1972

    authors: Umbreit JN,Strominger JL

    更新日期:1972-12-01 00:00:00

  • Implications of Tn5-associated adjacent deletions.

    abstract::The prokaryotic transposable element Tn5 has been found to promote the formation of adjacent deletions. The frequency of adjacent deletion formation is much lower than that of normal transposition events. Like normal transposition, however, adjacent deletion formation requires the activity of the transposase protein. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.5.1264-1271.1993

    authors: Jilk RA,Makris JC,Borchardt L,Reznikoff WS

    更新日期:1993-03-01 00:00:00

  • The Saccharomyces cerevisiae Isw2p-Itc1p complex represses INO1 expression and maintains cell morphology.

    abstract::In the yeast Saccharomyces cerevisiae, IRE1 encodes a bifunctional protein with transmembrane kinase and endoribonuclease activities. HAC1 encodes a transcription factor which has a basic leucine zipper domain. Both gene products play a crucial role in the unfolded protein response. Mutants in which one of these genes...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.183.17.4985-4993.2001

    authors: Sugiyama M,Nikawa J

    更新日期:2001-09-01 00:00:00