Chromosomal replication origins (oriC) of Enterobacter aerogenes and Klebsiella pneumoniae are functional in Escherichia coli.

abstract：:Cellular vanadium metabolism was studied in Saccharomyces cerevisiae by isolating and characterizing vanadate [VO4(3-), V(V)]-resistant mutants. Vanadate growth inhibition was reversed by the removal of the vanadate from the medium, and vanadate resistance was found to be a recessive trait. Vanadate-resistant mutants ...

journal_title：Journal of bacteriology

authors： Willsky GR,Leung JO,Offermann PV Jr,Plotnick EK,Dosch SF

更新日期：1985-11-01 00:00:00

abstract：:Activation of the late prespore-specific RNA polymerase sigma factor sigma(G) during Bacillus subtilis sporulation coincides with completion of the engulfment process, when the prespore becomes a protoplast fully surrounded by the mother cell cytoplasm and separated from it by a double membrane system. Activation of s...

journal_title：Journal of bacteriology

authors： Serrano M,Vieira F,Moran CP Jr,Henriques AO

更新日期：2008-12-01 00:00:00

abstract：:A 22-kb segment of chromosomal DNA from Enterococcus faecalis OG1RF containing the pyrimidine biosynthesis genes pyrC and pyrD was previously detected as complementing Escherichia coli pyrC and pyrD mutations. In the present study, it was found that the E. faecalis pyrimidine biosynthetic genes in this clone (designat...

journal_title：Journal of bacteriology

authors： Li X,Weinstock GM,Murray BE

更新日期：1995-12-01 00:00:00

abstract：:Novobiocin inhibited ribonculeic acid synthesis during germination of Bacillus subtilis spores. Transcription of certain kinds of genes probably required a preceding conformational change in deoxyribonucleic acid. ...

journal_title：Journal of bacteriology

authors： Matsuda M,Kameyama T

更新日期：1980-04-01 00:00:00

abstract：:The regulatory system that controls the expression of the Ti plasmid-borne octopine degradation (uad) and transfer (tra) genes in Agrobacterium tumefaciens was studied. A deletion mutant derived from the cointegrate plasmid R702::Ti-B6S3 was isolated, which was compatible with a wild-type Ti plasmid and which had reta...

journal_title：Journal of bacteriology

authors： Klapwijk PM,Schilperoort RA

更新日期：1979-08-01 00:00:00

abstract：:We describe here the identification and characterization of two Listeria monocytogenes (Tn917-LTV3) relA and hpt transposon insertion mutants that were impaired in growth after attachment to a model surface. Both mutants were unable to accumulate (p)ppGpp in response to amino acid starvation, whereas the wild-type str...

journal_title：Journal of bacteriology

authors： Taylor CM,Beresford M,Epton HA,Sigee DC,Shama G,Andrew PW,Roberts IS

更新日期：2002-02-01 00:00:00

abstract：:An R plasmid, R100-1, was mapped previously (Yoshikawa, 1974) by transduction from an integratively suppressed Hfr strain to a recipient with a mutation in gene dnaA. By this method various types of transductants of plasmid R100-1 that exist autonomously or in the integrated state were obtained. Seventy-one such trans...

journal_title：Journal of bacteriology

authors： Yoshikawa M

更新日期：1975-12-01 00:00:00

abstract：:In coupling the reducing power of illuminated chloroplasts to the nitrogenase from photosynthetically grown Rhodospirillum rubrum cells, one of the native ferredoxins. FdI, was found to be three times more effective than FdII. ...

journal_title：Journal of bacteriology

authors： Yoch DC,Arnon DI

更新日期：1975-02-01 00:00:00

abstract：:A 6.9-kilobase region of the Escherichia coli F plasmid containing the 3' half of the traD gene and the entire traI gene (encodes the TraI protein, DNA helicase I and TraI, a polypeptide arising from an internal in-frame translational start in traI) has been sequenced. A previously unidentified open reading frame (ten...

journal_title：Journal of bacteriology

authors： Bradshaw HD Jr,Traxler BA,Minkley EG Jr,Nester EW,Gordon MP

更新日期：1990-07-01 00:00:00

abstract：:The activation of the Pm promoter of the meta operon of the TOL plasmid of Pseudomonas putida by its cognate XylS activator protein in the presence and absence of benzoate inducers has been examined in specialized Escherichia coli strains carrying Pm-lacZ fusions and the xylS gene in different configurations in which ...

journal_title：Journal of bacteriology

authors： Kessler B,Herrero M,Timmis KN,de Lorenzo V

更新日期：1994-06-01 00:00:00

abstract：:An expressed sequence tag homologous to cheA was previously isolated by random sequencing of Thermotoga maritima cDNA clones (C. W. Kim, P. Markiewicz, J. J. Lee, C. F. Schierle, and J. H. Miller, J. Mol. Biol. 231: 960-981, 1993). Oligonucleotides complementary to this sequence tag were synthesized and used to identi...

journal_title：Journal of bacteriology

authors： Swanson RV,Sanna MG,Simon MI

更新日期：1996-01-01 00:00:00

abstract：:The dnaA operon of Escherichia coli contains the genes dnaA, dnaN, and recF encoding DnaA, beta clamp of DNA polymerase III holoenzyme, and RecF. When the DnaA concentration is raised, an increase in the number of DNA replication initiation events but a reduction in replication fork velocity occurs. Because DnaA is au...

journal_title：Journal of bacteriology

authors： Grigorian AV,Lustig RB,Guzmán EC,Mahaffy JM,Zyskind JW

更新日期：2003-01-01 00:00:00

abstract：:Two inhibitors of replicative deoxyribonucleic acid (DNA) synthesis, nalidixic acid (NAL) and 6-(p-hydroxyphenylazo)-uracil (HPUra), showed different effects on genetic recombination and DNA repair in Bacillus subtilis. Previous work (Pedrini et al., 1972) showed that NAL does not interfere with the transformation pro...

journal_title：Journal of bacteriology

authors： Canosi U,Siccardi AG,Falaschi A,Mazza G

更新日期：1976-04-01 00:00:00

abstract：:We isolated an Escherichia coli mutant strain that suppresses the glycolate-negative phenotype of a strain deficient in both GlcA and LldP transporters of this compound. This suppressing phenotype was assigned to yjcG, a gene whose function was previously unknown, which was found to encode a membrane protein able to t...

journal_title：Journal of bacteriology

authors： Gimenez R,Nuñez MF,Badia J,Aguilar J,Baldoma L

更新日期：2003-11-01 00:00:00

abstract：:Evidence from pulse-chase experiments indicates that the outer membrane matrix protein or porin of Escherichia coli B/r passes through a Sarkosyl-soluble membrane pool on the way to its eventual Sarkosyl-insoluble state in the outer membrane. ...

journal_title：Journal of bacteriology

authors： Boyd A,Holland IB

更新日期：1980-09-01 00:00:00

abstract：:The application of a simple negative staining technique and freeze-etching has revealed detailed information on the species-specific surface structure of spores and aerial hyphae of streptomycetes. ...

journal_title：Journal of bacteriology

authors： Wildermuth H

更新日期：1970-01-01 00:00:00

abstract：:In the chloroplasts of higher plants and algae, the biosynthesis of the chlorophyll precursor delta-aminolevulinic acid (ALA) involves at least three enzymes and a tRNA species. Here we demonstrate that in cell extracts of the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 ALA was formed from glutamate i...

journal_title：Journal of bacteriology

authors： O'Neill GP,Peterson DM,Schön A,Chen MW,Söll D

更新日期：1988-09-01 00:00:00

abstract：:In order to assess the roles of specific amino acid residues in the delta 5-3-ketosteroid isomerase from Pseudomonas putida biotype B during catalysis, we replaced aspartic acid 40 with asparagine (D40N) and tyrosine 16 with phenylalanine (Y16F) in the enzyme by site-directed mutagenesis. Both purified mutant enzymes ...

journal_title：Journal of bacteriology

authors： Kim SW,Choi KY

更新日期：1995-05-01 00:00:00

abstract：:Plasmid pE194 has been shown to be rescued by integration after cultivation of infected Bacillus subtilis recE4 cells at a restrictive high temperature. The plasmid is also spontaneously excised from the chromosome at a low frequency by precise or imprecise excision (J. Hofemeister, M. Israeli-Reches, and D. Dubnau, M...

journal_title：Journal of bacteriology

authors： Conrad B,Bashkirov VI,Hofemeister J

更新日期：1992-11-01 00:00:00

abstract：:Among mutants which require isoleucine, but not valine, for growth, we have found two distinguishable classes. One is defective in the biosynthetic enzyme threonine deaminase (l-threonine hydro-lyase, deaminating, EC 4.2.1.16) and the other has an altered isoleucyl transfer ribonucleic acid (tRNA) synthetase [l-isoleu...

journal_title：Journal of bacteriology

authors： Iaccarino M,Berg P

更新日期：1971-02-01 00:00:00

abstract：:We report the draft genome of the strain Lactobacillus gigeriorum CRBIP 24.85(T), isolated from a chicken crop. The total length of the 60 scaffolds is about 1.9 Mb, with a GC content of 38% and 2,062 protein-coding sequences (CDS). ...

journal_title：Journal of bacteriology

authors： Cousin S,Ma L,Creno S,Clermont D,Loux V,Bizet C,Bouchier C

更新日期：2012-11-01 00:00:00

abstract：:The regulatory proteins OmpR and EnvZ are both required to activate expression of the genes for the major outer membrane porin proteins, OmpF and OmpC, of Escherichia coli K-12. Here we show that OmpR, under certain conditions, could activate porin expression in the complete absence of EnvZ. In addition, the pleiotrop...

journal_title：Journal of bacteriology

authors： Slauch JM,Garrett S,Jackson DE,Silhavy TJ

更新日期：1988-01-01 00:00:00

abstract：:Differential expression of genes within the puf photosynthesis operon of Rhodobacter capsulatus is achieved primarily through marked segmental differences in stability within the polycistronic puf operon transcripts. The comparatively stable pufBA segment of these transcripts outlives the labile pufLMX segment and acc...

journal_title：Journal of bacteriology

authors： Chen CY,Belasco JG

更新日期：1990-08-01 00:00:00

abstract：:31P and 13C nuclear magnetic resonance (NMR) experiments were performed on suspensions of the phototrophic bacterium Chromatium vinosum incubated anaerobically in the dark. 31P NMR spectra revealed that during prolonged dark incubation high ATP levels are maintained. This phenomenon was independent of the presence of ...

journal_title：Journal of bacteriology

authors： Nicolay K,van Gemerden H,Hellingwerf KJ,Konings WN,Kaptein R

更新日期：1983-08-01 00:00:00

abstract：:Translational efficiency of an AUG, CUG, GUG, or UUG initiation codon was measured for the naturally leaderless cI mRNA from bacteriophage lambda. In a cI-lacZ translational fusion, only AUG supported a high level of expression; GUG supported a low level of expression, while UUG and CUG expression was barely above bac...

journal_title：Journal of bacteriology

authors： O'Donnell SM,Janssen GR

更新日期：2001-02-01 00:00:00

abstract：:Escherichia coli sigma70-dependent promoters have typically been characterized as either -10/-35 promoters, which have good matches to both the canonical -10 and the -35 sequences or as extended -10 promoters (TGn/-10 promoters), which have the TGn motif and an excellent match to the -10 consensus sequence. We report ...

journal_title：Journal of bacteriology

authors： Hook-Barnard I,Johnson XB,Hinton DM

更新日期：2006-12-01 00:00:00

abstract：:Bacteria of the genus Prosthecobacter express homologs of eukaryotic α- and β-tubulin, called BtubA and BtubB (BtubA/B), that have been observed to assemble into filaments in the presence of GTP. BtubA/B polymers are proposed to be composed in vitro by two to six protofilaments in contrast to that in vivo, where they ...

journal_title：Journal of bacteriology

authors： Díaz-Celis C,Risca VI,Hurtado F,Polka JK,Hansen SD,Maturana D,Lagos R,Mullins RD,Monasterio O

更新日期：2017-09-05 00:00:00

abstract：:Chloramphenicol-resistant strains of Staphylococcus aureus contain an inducible enzyme which inactivates chloramphenicol by acetylation in the presence of acetyl coenzyme A. The products of acetylation are chromatographically indistinguishable from those obtained with chloramphenicol-resistant Escherichia coli harbori...

journal_title：Journal of bacteriology

authors： Shaw WV,Brodsky RF

更新日期：1968-01-01 00:00:00

abstract：:In this study, we cloned the Pseudomonas aeruginosa zwf gene, encoding glucose-6-phosphate dehydrogenase (G6PDH), an enzyme that catalyzes the NAD+- or NADP+-dependent conversion of glucose-6-phosphate to 6-phosphogluconate. The predicted zwf gene product is 490 residues, which could form a tetramer with a molecular m...

journal_title：Journal of bacteriology

authors： Ma JF,Hager PW,Howell ML,Phibbs PV,Hassett DJ

更新日期：1998-04-01 00:00:00

abstract：:The poly-beta-hydroxybutyrate (PHB) biosynthetic pathway from Alcaligenes eutrophus H16 has been cloned and expressed in Escherichia coli. Initially, an A. eutrophus H16 genomic library was constructed by using cosmid pVK102, and cosmid clones that encoded the PHB biosynthetic pathway were sought by assaying for the f...

journal_title：Journal of bacteriology

authors： Slater SC,Voige WH,Dennis DE

更新日期：1988-10-01 00:00:00