Experimental evolution of penicillin G acylases from Escherichia coli and Proteus rettgeri.

Abstract:

:Proteus rettgeri and Escherichia coli W were shown to express structurally different penicillin G acylases. The enzymes had similar substrate specificity but differed in molecular weight, isoelectric point, and electrophoretic mobility in polyacrylamide gels and did not antigenically cross-react. When the organisms were subjected to environmental conditions which made expression of this enzyme essential for growth, spontaneous mutants were isolated that used different amides as the only source of nitrogen. These mutants acquired the ability to use amides for growth by deregulating the penicillin G acylase and by their evolution to novel substrate specificities. The enzymes expressed by mutants isolated from each genus appeared to have evolved in parallel since each acylase attained similar new substrate specificities when the organisms were subjected to identical selection pressure.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Daumy GO,Danley D,McColl AS,Apostolakos D,Vinick FJ

doi

10.1128/JB.163.3.925-932.1985

subject

Has Abstract

pub_date

1985-09-01 00:00:00

pages

925-32

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

163

pub_type

杂志文章
  • Staphylococcal Muller phenomenon: relationship to the plasminogen-plasmin system.

    abstract::Quie, Paul G. (University of Minnesota, Minneapolis) and Lewis W. Wannamaker. Staphylococcal Muller phenomenon: relationship to the plasminogen-plasmin system. J. Bacteriol. 82:770-783. 1961.-The staphylococcal factor that produces particulate proteolysis (Muller phenomenon) in whole blood or hemoglobinserum agar plat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.82.5.770-783.1961

    authors: QUIE PG,WANNAMAKER LW

    更新日期:1961-11-01 00:00:00

  • ssrA (tmRNA) plays a role in Salmonella enterica serovar Typhimurium pathogenesis.

    abstract::Escherichia coli ssrA encodes a small stable RNA molecule, tmRNA, that has many diverse functions, including tagging abnormal proteins for degradation, supporting phage growth, and modulating the activity of DNA binding proteins. Here we show that ssrA plays a role in Salmonella enterica serovar Typhimurium pathogenes...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.6.1558-1563.2000

    authors: Julio SM,Heithoff DM,Mahan MJ

    更新日期:2000-03-01 00:00:00

  • The pSC101 par locus alters protein-DNA interactions in vivo at the plasmid replication origin.

    abstract::We report here direct evidence that mutations in the par locus affect protein-DNA interactions in vivo at the replication origin of plasmid pSC101. Concomitant with par-mediated plasmid stabilization, two sites in the origin region show an altered methylation pattern as detected by in vivo footprinting with dimethyl s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.18.6046-6048.1993

    authors: Ingmer H,Cohen SN

    更新日期:1993-09-01 00:00:00

  • Plasmid-encoded regulator of extracellular proteases in Bacillus anthracis.

    abstract::Bacillus anthracis Sterne cured of the pXO1 plasmid had enhanced secreted protease activity during the postexponential phase but no change in hemolytic or lecithinase activities. A zymogen profile revealed at least six proteases, including serine, metal, and perhaps cysteine types. There were similar amounts of protea...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.9.3133-3138.2005

    authors: Aronson AI,Bell C,Fulroth B

    更新日期:2005-05-01 00:00:00

  • Functional analysis of luxS in Staphylococcus aureus reveals a role in metabolism but not quorum sensing.

    abstract::The function of AI-2 in many bacteria and the physiological role of LuxS, the enzyme responsible for its production, remain matters of debate. Here, we show that in Staphylococcus aureus the luxS gene forms a monocistronic transcriptional unit under the control of a sigma(70)-dependent promoter. The gene was transcrib...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.8.2885-2897.2006

    authors: Doherty N,Holden MT,Qazi SN,Williams P,Winzer K

    更新日期:2006-04-01 00:00:00

  • Characterization of a zinc-containing alcohol dehydrogenase with stereoselectivity from the hyperthermophilic archaeon Thermococcus guaymasensis.

    abstract::An alcohol dehydrogenase (ADH) from hyperthermophilic archaeon Thermococcus guaymasensis was purified to homogeneity and was found to be a homotetramer with a subunit size of 40 ± 1 kDa. The gene encoding the enzyme was cloned and sequenced; this gene had 1,095 bp, corresponding to 365 amino acids, and showed high seq...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01433-10

    authors: Ying X,Ma K

    更新日期:2011-06-01 00:00:00

  • A four-dimensional view of assembly of a morphogenetic protein during sporulation in Bacillus subtilis.

    abstract::We report the use of a fusion to the green fluorescent protein to visualize the assembly of the morphogenetic protein SpoIVA around the developing forespore during the process of sporulation in the bacterium Bacillus subtilis. Using a deconvolution algorithm to process digitally-collected optical sections, we show tha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.3.781-790.1999

    authors: Price KD,Losick R

    更新日期:1999-02-01 00:00:00

  • Changes in ribosomal activity of Escherichia coli cells during prolonged culture in sea salts medium.

    abstract::The activity of ribosomes from a clinical isolate of Escherichia coli, exposed to starvation for 7 days in sea salts medium, was investigated by measuring the kinetic parameters of ribosomal peptidyltransferase, by using the puromycin reaction as a model reaction. No alterations in the extent of peptide bond formation...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.12.3114-3119.1998

    authors: Kalpaxis DL,Karahalios P,Papapetropoulou M

    更新日期:1998-06-01 00:00:00

  • Identification and characterization of two chemotactic transducers for inorganic phosphate in Pseudomonas aeruginosa.

    abstract::Two chemotactic transducers for inorganic phosphate (P(i)), designated CtpH and CtpL, have been identified in Pseudomonas aeruginosa. The corresponding genes (ctpH and ctpL) were inactivated by inserting kanamycin and tetracycline resistance gene cassettes into the wild-type genes in the P. aeruginosa PAO1 genome. Com...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.12.3400-3404.2000

    authors: Wu H,Kato J,Kuroda A,Ikeda T,Takiguchi N,Ohtake H

    更新日期:2000-06-01 00:00:00

  • Stability of beta-galactosidase messenger ribonucleic acid in Escherichia coli.

    abstract::Ben-Hamida, Fakher (Washington University School of Medicine, St. Louis, Mo.), and David Schlessinger. Stability of beta-galactosidase messenger ribonucleic acid in Escherichia coli. J. Bacteriol. 90:1611-1616. 1965.-Synthesis of beta-galactosidase stops within several minutes when preinduced, permeaseless cultures ar...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.90.6.1611-1616.1965

    authors: Ben-Hamida F,Schlessinger D

    更新日期:1965-12-01 00:00:00

  • Analytical studies on regeneration of protoplasts of Geotrichum candidum by quantitative thin-layer-agar plating.

    abstract::A preparation of pure protoplasts of Geotrichum candidum became osmotically stable and colonies developed when the protoplasts were embedded in stabilizing thin-layer-agar and incubated with stabilizing basal medium. When growing protoplasts were exposed to distilled water and then reincubated with basal medium, the p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.1.256-263.1969

    authors: Fukui K,Sagara Y,Yoshida N,Matsuoka T

    更新日期:1969-04-01 00:00:00

  • Molecular mapping of glyW, a duplicate gene for tRNA3Gly of Escherichia coli.

    abstract::By the use of [5'-32P]tRNA3Gly from Escherichia coli as a hybridization probe, glyW was located on cloned fragments of the uvrC pgsA region of the bacterial chromosome. After determination of the sites of action of several restriction enzymes, glyW was found to be within approximately 300 base pairs of pgsA. The order...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Tucker SD,Gopalakrishnan AS,Bollinger R,Dowhan W,Murgola EJ

    更新日期:1982-11-01 00:00:00

  • Two RecA protein types that mediate different modes of hyperrecombination.

    abstract::RecAX53 is a chimeric variant of the Escherichia coli RecA protein (RecAEc) that contains a part of the central domain of Pseudomonas aeruginosa RecA (RecAPa), encompassing a region that differs from RecAEc at 12 amino acid positions. Like RecAPa, this chimera exhibits hyperrecombination activity in E. coli cells, inc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01006-07

    authors: Baitin DM,Bakhlanova IV,Chervyakova DV,Kil YV,Lanzov VA,Cox MM

    更新日期:2008-04-01 00:00:00

  • Lon Protease Removes Excess Signal Recognition Particle Protein in Escherichia coli.

    abstract::Correct targeting of membrane proteins is essential for membrane integrity, cell physiology, and viability. Cotranslational targeting depends on the universally conserved signal recognition particle (SRP), which is a ribonucleoprotein complex comprised of the protein component Ffh and the 4.5S RNA in Escherichia coli ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00161-20

    authors: Sauerbrei B,Arends J,Schünemann D,Narberhaus F

    更新日期:2020-06-25 00:00:00

  • Corrected Genome Annotations Reveal Gene Loss and Antibiotic Resistance as Drivers in the Fitness Evolution of Salmonella enterica Serovar Typhimurium.

    abstract::Horizontal acquisition of novel chromosomal genes is considered to be a key process in the evolution of bacterial pathogens. However, the identification of gene presence or absence could be hindered by the inconsistencies in bacterial genome annotations. Here, we performed a cross-annotation of omnipresent core and mo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00545-16

    authors: Paul S,Sokurenko EV,Chattopadhyay S

    更新日期:2016-11-04 00:00:00

  • The ferrichrome uptake pathway in Pseudomonas aeruginosa involves an iron release mechanism with acylation of the siderophore and recycling of the modified desferrichrome.

    abstract::The uptake of iron into Pseudomonas aeruginosa is mediated by two major siderophores produced by the bacterium, pyoverdine and pyochelin. The bacterium is also able of utilize several heterologous siderophores of bacterial or fungal origin. In this work, we have investigated the iron uptake in P. aeruginosa PAO1 by th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01539-09

    authors: Hannauer M,Barda Y,Mislin GL,Shanzer A,Schalk IJ

    更新日期:2010-03-01 00:00:00

  • Influence of methionine pool composition on the formation of methyl-deficient transfer ribonucleic acid in Saccharomyces cerevisiae.

    abstract::Methionine auxotrophs of Saccharomyces cerevisiae continue to synthesize ribonucleic acid (RNA) after methionine withdrawal. The newly synthesized transfer RNA (tRNA) is methyl-deficient in some strains, but not in all. Whether such tRNA will accumulate depends on the position of the block in the methionine pathway th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.2.687-694.1969

    authors: Kjellin-Stråby K

    更新日期:1969-11-01 00:00:00

  • Characterization of the autocleavage process of the Escherichia coli HtrA protein: implications for its physiological role.

    abstract::The Escherichia coli HtrA protein is a periplasmic protease/chaperone that is upregulated under stress conditions. The protease and chaperone activities of HtrA eliminate or refold damaged and unfolded proteins in the bacterial periplasm that are generated upon stress conditions. In the absence of substrates, HtrA oli...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01187-08

    authors: Jomaa A,Iwanczyk J,Tran J,Ortega J

    更新日期:2009-03-01 00:00:00

  • Electron probe analysis, X-ray mapping, and electron energy-loss spectroscopy of calcium, magnesium, and monovalent ions in log-phase and in dividing Escherichia coli B cells.

    abstract::The elemental composition of individual cells of rapidly frozen and cryosectioned Escherichia coli B was measured with electron optical microanalytic methods. The Ca content was high (26.2 mmol/kg) in a 10-nm-wide region of the cell envelope. Amounts of cytoplasmic Ca in actively dividing cells were significantly high...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.167.3.935-939.1986

    authors: Chang CF,Shuman H,Somlyo AP

    更新日期:1986-09-01 00:00:00

  • Mu and IS1 transpositions exhibit strong orientation bias at the Escherichia coli bgl locus.

    abstract::The region upstream of the Escherichia coli bgl operon is an insertion hot spot for several transposons. Elements as distantly related as Tn1, Tn5, and phage Mu home in on this location. To see what characteristics result in a high-affinity site for transposition, we compared in vivo and in vitro Mu transposition patt...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.11.3328-3335.2001

    authors: Manna D,Wang X,Higgins NP

    更新日期:2001-06-01 00:00:00

  • Contribution of the periplasmic chaperone Skp to efficient presentation of the autotransporter IcsA on the surface of Shigella flexneri.

    abstract::IcsA is an outer membrane protein in the autotransporter family that is required for Shigella flexneri pathogenesis. Following its secretion through the Sec translocon, IcsA is incorporated into the outer membrane in a process that depends on YaeT, a component of an outer membrane beta-barrel insertion machinery. We i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00989-08

    authors: Wagner JK,Heindl JE,Gray AN,Jain S,Goldberg MB

    更新日期:2009-02-01 00:00:00

  • The Streptococcal Protease SpeB Antagonizes the Biofilms of the Human Pathogen Staphylococcus aureus USA300 through Cleavage of the Staphylococcal SdrC Protein.

    abstract::Streptococcus pyogenes, or group A Streptococcus (GAS), is both a pathogen and an asymptomatic colonizer of human hosts and produces a large number of surface-expressed and secreted factors that contribute to a variety of infection outcomes. The GAS-secreted cysteine protease SpeB has been well studied for its effects...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00008-20

    authors: Carothers KE,Liang Z,Mayfield J,Donahue DL,Lee M,Boggess B,Ploplis VA,Castellino FJ,Lee SW

    更新日期:2020-05-11 00:00:00

  • Environmental role of pathogenic traits in Vibrio cholerae.

    abstract::Vibrio cholerae is a natural inhabitant of aquatic ecosystems. Some strains of V. cholerae can colonize the human host and cause cholera, a profuse watery diarrhea. The major pathogenicity factors and virulence regulators of V. cholerae are either encoded in mobile genetic elements acquired in the environment (e.g. pa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00795-17

    authors: Sakib SN,Reddi G,Almagro-Moreno S

    更新日期:2018-03-26 00:00:00

  • Measurement of adhesive forces between individual Staphylococcus aureus MSCRAMMs and protein-coated surfaces by use of optical tweezers.

    abstract::The force required to rupture bonds between individual Staphylococcus aureus MSCRAMMs and surfaces coated with extracellular matrix molecules has been quantified by using optical tweezers. The observed binding forces between fibrinogen or fibronectin and S. aureus MSCRAMMs occurred as an approximate integer multiple o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.6.2031-2035.2003

    authors: Simpson KH,Bowden G,Höök M,Anvari B

    更新日期:2003-03-01 00:00:00

  • Ultrastructure of Treponema microdentium and Borrelia vincentii.

    abstract::Bladen, Howard A. (National Institute of Dental Health, Bethesda, Md.), and Edward G. Hampp. Ultrastructure of Treponema microdentium and Borrelia vincentii. J. Bacteriol. 87:1180-1191.-A small oral Treponema (FM) and Borrelia vincentii (N9) were harvested after 3 to 7 days of incubation and either embedded in Vestopa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.5.1180-1191.1964

    authors: Bladen HA,Hampp EG

    更新日期:1964-05-01 00:00:00

  • Prediction of the rate of glucose utilization from cellular levels of glucose 6-phosphate and fructose 1,6-diphosphate in Escherichia coli.

    abstract::A comprehensive equation, upsilon = VM/[1 + (A0.5/Fru-P2)n] [ 1 + (Glc-6-P/I0.5)], has been proposed to represent the quantitative interrelationships between the rate of glucose utilization and the levels of glucose-6-phosphate and fructose-1,6-diphosphate in the intact Escherichia coli cell. This comprehensive equati...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.128.1.165-169.1976

    authors: Dietzler DN,Leckie MP,Crimmins DL,Ungar JM,Sternheim WL

    更新日期:1976-10-01 00:00:00

  • Mutational analysis of transmembrane regions 3 and 4 of SecY, a central component of protein translocase.

    abstract::The SecYEG heterotrimeric membrane protein complex functions as a channel for protein translocation across the Escherichia coli cytoplasmic membrane. SecY is the central subunit of the SecYEG complex and contains 10 transmembrane segments (TM1 to TM10). Previous mutation studies suggested that TM3 and TM4 are particul...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.12.3960-3969.2004

    authors: Mori H,Shimokawa N,Satoh Y,Ito K

    更新日期:2004-06-01 00:00:00

  • Electron acceptor taxis and blue light effect on bacterial chemotaxis.

    abstract::Salmonella typhimurium and Escherichia coli from anaerobic cultures displayed tactic responses to gradients of nitrate, fumarate, and oxygen when the appropriate electron transport pathway was present. Such responses were named "electron acceptor taxis" because they are elicited by terminal electron acceptors. Mutant ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.2.567-573.1979

    authors: Taylor BL,Miller JB,Warrick HM,Koshland DE Jr

    更新日期:1979-11-01 00:00:00

  • EslB Is Required for Cell Wall Biosynthesis and Modification in Listeria monocytogenes.

    abstract::Lysozyme is an important component of the innate immune system. It functions by hydrolyzing the peptidoglycan (PG) layer of bacteria. The human pathogen Listeria monocytogenes is intrinsically lysozyme resistant. The peptidoglycan N-deacetylase PgdA and O-acetyltransferase OatA are two known factors contributing to it...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00553-20

    authors: Rismondo J,Schulz LM,Yacoub M,Wadhawan A,Hoppert M,Dionne MS,Gründling A

    更新日期:2021-01-25 00:00:00

  • Characterization of the Pseudomonas aeruginosa transcriptional response to phenylalanine and tyrosine.

    abstract::Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen often associated with chronic infections in the lungs of individuals with the heritable disease cystic fibrosis (CF). Previous work from our laboratory demonstrated that aromatic amino acids within CF lung secretions (sputum) not only serve as carbon and...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00112-10

    authors: Palmer GC,Palmer KL,Jorth PA,Whiteley M

    更新日期:2010-06-01 00:00:00