Abstract:
:In Escherichia coli K-12, expression of the lysU gene is regulated by the lrp gene product, as indicated by an increase in the level of lysyl-tRNA synthetase activity and LysU protein in an lrp mutant. Comparison of the patterns of protein expression visualized by two-dimensional gel electrophoresis indicated that LysU is present at higher levels in an lrp strain than in its isogenic lrp+ parent. The purified lrp gene product was shown to bind to sites upstream of the lysU gene and to protect several sites against DNase I digestion. A region extending over 100 nucleotides, between 60 and 160 nucleotides upstream from the start of the lysU coding sequence, showed altered sensitivity to DNase I digestion in the presence of the Lrp protein. The extent of protected DNA suggests a complex interaction of Lrp protein and upstream lysU DNA.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Lin R,Ernsting B,Hirshfield IN,Matthews RG,Neidhardt FC,Clark RL,Newman EBdoi
10.1128/jb.174.9.2779-2784.1992keywords:
subject
Has Abstractpub_date
1992-05-01 00:00:00pages
2779-84issue
9eissn
0021-9193issn
1098-5530journal_volume
174pub_type
杂志文章abstract::We used primer extension and mutational analysis to identify a promoter upstream of ilvB, the first gene in the ilv-leu operon of Bacillus subtilis. Between the promoter and ilvB, there is a 482-bp leader region which contains a sequence that resembles a factor-independent transcription terminator. In in vitro transcr...
journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2017-07-25 00:00:00
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更新日期:1974-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.165.1.336-339.1986
更新日期:1986-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1970-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1986-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.127.2.755-762.1976
更新日期:1976-08-01 00:00:00