The lrp gene product regulates expression of lysU in Escherichia coli K-12.

Abstract:

:In Escherichia coli K-12, expression of the lysU gene is regulated by the lrp gene product, as indicated by an increase in the level of lysyl-tRNA synthetase activity and LysU protein in an lrp mutant. Comparison of the patterns of protein expression visualized by two-dimensional gel electrophoresis indicated that LysU is present at higher levels in an lrp strain than in its isogenic lrp+ parent. The purified lrp gene product was shown to bind to sites upstream of the lysU gene and to protect several sites against DNase I digestion. A region extending over 100 nucleotides, between 60 and 160 nucleotides upstream from the start of the lysU coding sequence, showed altered sensitivity to DNase I digestion in the presence of the Lrp protein. The extent of protected DNA suggests a complex interaction of Lrp protein and upstream lysU DNA.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Lin R,Ernsting B,Hirshfield IN,Matthews RG,Neidhardt FC,Clark RL,Newman EB

doi

10.1128/jb.174.9.2779-2784.1992

keywords:

subject

Has Abstract

pub_date

1992-05-01 00:00:00

pages

2779-84

issue

9

eissn

0021-9193

issn

1098-5530

journal_volume

174

pub_type

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