Sucrose-dependent spectinomycin-resistant mutants of Escherichia coli.

abstract：:Structural studies were carried out on lipoteichoic acids obtained from defatted cells of 10 Bacillus strains by phenol-water partition followed by chromatography on DEAE-Sephacel and Octyl-Sepharose columns. A group of the tested bacteria (group A), Bacillus subtilis, Bacillus licheniformis, and Bacillus pumilus, was...

journal_title：Journal of bacteriology

authors： Iwasaki H,Shimada A,Ito E

更新日期：1986-08-01 00:00:00

abstract：:The conjugally acquired deoxyribonucleic acid (DNA) of small, anucleate cells ("minicells") of a mutant strain of Escherichia coli K-12 was found to be predominantly associated with the bacterial membrane. Evidence from X-irradiation studies in vivo shows that there is no decrease in DNA-membrane association under con...

journal_title：Journal of bacteriology

authors： Shull FW Jr,Fralick JA,Stratton LP,Fisher WD

更新日期：1971-05-01 00:00:00

abstract：UNLABELLED:Rhodopseudomonas palustris is an alphaproteobacterium that has served as a model organism for studies of photophosphorylation, regulation of nitrogen fixation, production of hydrogen as a biofuel, and anaerobic degradation of aromatic compounds. This bacterium is able to transition between anaerobic photoaut...

journal_title：Journal of bacteriology

authors： Pechter KB,Gallagher L,Pyles H,Manoil CS,Harwood CS

更新日期：2015-12-28 00:00:00

abstract：:The DnaK protein of Zymomonas mobilis (DnaKz) was identified and found to be 80% identical to the DnaK protein of Escherichia coli on the basis of the sequence of the N-terminal 21 amino acids. The dnaKz gene was cloned and found to be expressed in a thermosensitive dnaK mutant of Escherichia coli. Expression of the f...

journal_title：Journal of bacteriology

authors： Michel GP

更新日期：1993-05-01 00:00:00

abstract：:S-Adenosyl-L-methionine:precorrin-2 methyltransferase (SP2MT), which catalyzes the C-20 methylation of precorrin-2 to precorrin-3, was purified to homogeneity from extracts of a recombinant strain of Pseudomonas denitrificans derived from a cobalamin-overproducing strain. Ammonium sulfate fractionation followed by chr...

journal_title：Journal of bacteriology

authors： Thibaut D,Couder M,Crouzet J,Debussche L,Cameron B,Blanche F

更新日期：1990-11-01 00:00:00

abstract：:Alkaline phosphatase (APase) is induced as a culture enters stationary phase because of limiting phosphate. The results presented here show that expression of APase is regulated both negatively and positively. PhoP, a homolog of a family of bacterial transcription factors, and PhoR, a homolog of bacterial histidine pr...

journal_title：Journal of bacteriology

authors： Jensen KK,Sharkova E,Duggan MF,Qi Y,Koide A,Hoch JA,Hulett FM

更新日期：1993-06-01 00:00:00

abstract：:Xylose is rarely described as a component of bacterial glycans. UDP-xylose is the nucleotide-activated form necessary for incorporation of xylose into glycans and is synthesized by the decarboxylation of UDP-glucuronic acid (UDP-GlcA). Enzymes with UDP-GlcA decarboxylase activity include those that lead to the formati...

journal_title：Journal of bacteriology

authors： Coyne MJ,Fletcher CM,Reinap B,Comstock LE

更新日期：2011-10-01 00:00:00

abstract：:Cell-free extracts of Thermus thermophilus HB8 catalyze the in vitro, NADH-dependent reduction of potassium tellurite (K2TeO3). Three different protein fractions with tellurite-reducing activities were identified. Two exhibited high molecular weight and were composed of at least two different polypeptides. The protein...

journal_title：Journal of bacteriology

authors： Chiong M,González E,Barra R,Vásquez C

更新日期：1988-07-01 00:00:00

abstract：:The Escherichia coli F plasmid gene required for amino-terminal acetylation of F-pilin subunits was identified. Using Western blots (immunoblots), we assayed the reaction of monoclonal antibodies with F-pilin polypeptides in inner membrane preparations from various F mutant strains. It was known that JEL92 recognizes ...

journal_title：Journal of bacteriology

authors： Moore D,Hamilton CM,Maneewannakul K,Mintz Y,Frost LS,Ippen-Ihler K

更新日期：1993-03-01 00:00:00

abstract：:Agrobacterium tumefaciens and Rhizobium meliloti carry related genetic loci which have important roles in virulence and symbiosis. Previously, it was shown that two virulence loci of A. tumefaciens, chvA and chvB, are related to two R. meliloti symbiosis loci, ndvA and ndvB, respectively (T. Dylan, L. Ielpi, S. Stanfi...

journal_title：Journal of bacteriology

authors： Marks JR,Lynch TJ,Karlinsey JE,Thomashow MF

更新日期：1987-12-01 00:00:00

abstract：:Staphylococcus lugdunensis is the only coagulase-negative Staphylococcus species with a locus encoding iron-regulated surface determinant (Isd) proteins. In Staphylococcus aureus, the Isd proteins capture heme from hemoglobin and transfer it across the wall to a membrane-bound transporter, which delivers it into the c...

journal_title：Journal of bacteriology

authors： Zapotoczna M,Heilbronner S,Speziale P,Foster TJ

更新日期：2012-12-01 00:00:00

abstract：:This is the first complete genome sequence of the Enterobacter aerogenes species. Here we present the genome sequence of E. aerogenes KCTC 2190, which contains 5,280,350 bp with a G + C content of 54.8 mol%, 4,912 protein-coding genes, and 109 structural RNAs. ...

journal_title：Journal of bacteriology

authors： Shin SH,Kim S,Kim JY,Lee S,Um Y,Oh MK,Kim YR,Lee J,Yang KS

更新日期：2012-05-01 00:00:00

abstract：:Virulence genes in Listeria monocytogenes are coordinately expressed under the control of the transcriptional activator PrfA, encoded by prfA, a member of the cyclic AMP (cAMP) receptor protein (CRP)/FNR family of bacterial regulators. Strain P14-A is a spontaneous mutant of L. monocytogenes serovar 4b which produces ...

journal_title：Journal of bacteriology

authors： Ripio MT,Domínguez-Bernal G,Lara M,Suárez M,Vazquez-Boland JA

更新日期：1997-03-01 00:00:00

abstract：:Several spontaneous cya and crp mutants of Escherichia coli have been selected as clones simultaneously resistant to phage lambda and nalidixic acid and characterized. Both cya and crp mutants have been found to grow as cocci with increased doubling times. They have increased resistance to some mutagens (methylmethane...

journal_title：Journal of bacteriology

authors： Kumar S

更新日期：1976-02-01 00:00:00

abstract：:Although 95% of the enterotoxin B produced by Staphylococcus aureus appears during the latter part of the exponential phase of growth, growth per se is not necessary for toxin synthesis. A procedure is described whereby a concentrated suspension (at least 6 x 10(10) cells per ml) of a 16-hr culture of S. aureus was fo...

journal_title：Journal of bacteriology

authors： Markus Z,Silverman GJ

更新日期：1969-02-01 00:00:00

abstract：:The dnaA genes of Salmonella typhimurium and Serratia marcescens, which complemented the temperature-sensitive dnaA46 mutation of Escherichia coli, were cloned and sequenced. They were very homologous to the dnaA gene of E. coli. The 63 N-terminal amino acids and the 333 C-terminal amino acids of the corresponding Dna...

journal_title：Journal of bacteriology

authors： Skovgaard O,Hansen FG

更新日期：1987-09-01 00:00:00

abstract：:A combined physical and genetic map of the genome of strain SG24 of Vibrio cholerae O139 Bengal, a novel non-O1 strain having epidemic potential, has been constructed by using the enzymes NotI, SfiI, and CeuI. The genome of SG24 is circular, and the genome size is about 3. 57 Mb. The linkages between 47 NotI and 32 Sf...

journal_title：Journal of bacteriology

authors： Khetawat G,Bhadra RK,Kar S,Das J

更新日期：1998-09-01 00:00:00

abstract：:Streptococcus suis serotype 2 is a highly invasive, extracellular pathogen in pigs with the capacity to cause severe infections in humans. This study was initiated by the finding that IgM degradation products are released after opsonization of S. suis. The objective of this work was to identify the bacterial factor re...

journal_title：Journal of bacteriology

authors： Seele J,Singpiel A,Spoerry C,von Pawel-Rammingen U,Valentin-Weigand P,Baums CG

更新日期：2013-03-01 00:00:00

abstract：:The cloned gene coding for Bacillus licheniformis penicillinase (penP) was introduced into Escherichia coli in a heat-inducible lambda Qam vector. After induction, significant amounts of penicillinase were synthesized in the new host. The cellular location of the penicillinase was found to be almost exclusively the ou...

journal_title：Journal of bacteriology

authors： Sarvas MO,Palva IA

更新日期：1983-08-01 00:00:00

abstract：:The elongation factor Tu, encoded by tuf genes, is a GTP binding protein that plays a central role in protein synthesis. One to three tuf genes per genome are present, depending on the bacterial species. Most low-G+C-content gram-positive bacteria carry only one tuf gene. We have designed degenerate PCR primers derive...

journal_title：Journal of bacteriology

authors： Ke D,Boissinot M,Huletsky A,Picard FJ,Frenette J,Ouellette M,Roy PH,Bergeron MG

更新日期：2000-12-01 00:00:00

abstract：:A rare group I intron in a cyanobacterial ribonucleotide reductase gene has been characterized. It contains a mobile insertion sequence element not required for RNA splicing. Ribonucleotide reductase genes were found to be hot spots for all three types of self-splicing intervening sequences, including group I and II i...

journal_title：Journal of bacteriology

authors： Meng Q,Zhang Y,Liu XQ

更新日期：2007-03-01 00:00:00

abstract：:We investigated the effect of time, temperature, and the presence of sodium chloride, nitrates, and nitrites in the medium on the growth and production of enterotoxin B by Staphylococcus aureus. Assays by the double gel-diffusion method showed that maximal enterotoxin B production occurs at the beginning of the statio...

journal_title：Journal of bacteriology

authors： McLean RA,Lilly HD,Alford JA

更新日期：1968-04-01 00:00:00

abstract：:The effects of competence-inducing treatments on the composition and organization of membrane lipids in Escherichia coli K-12, DH1, DH5, HB101, and RR1 were investigated for two widely used protocols in which transformability is developed at low temperatures in Ca2+ buffers. At stages during each procedure, the lipid ...

journal_title：Journal of bacteriology

authors： Reusch RN,Hiske TW,Sadoff HL

更新日期：1986-11-01 00:00:00

abstract：:Neisseria meningitidis can be the causative agent of meningitis or septicemia. This bacterium expresses type IV pili, which mediate a variety of functions, including autoagglutination, twitching motility, biofilm formation, adherence, and DNA uptake during transformation. The secretin PilQ supports type IV pilus extru...

journal_title：Journal of bacteriology

authors： Balasingham SV,Collins RF,Assalkhou R,Homberset H,Frye SA,Derrick JP,Tønjum T

更新日期：2007-08-01 00:00:00

abstract：:Beta toxin is a neutral sphingomyelinase secreted by certain strains of Staphylococcus aureus. This virulence factor lyses erythrocytes in order to evade the host immune system as well as scavenge nutrients. The structure of beta toxin was determined at 2.4-A resolution using crystals that were merohedrally twinned. T...

journal_title：Journal of bacteriology

authors： Huseby M,Shi K,Brown CK,Digre J,Mengistu F,Seo KS,Bohach GA,Schlievert PM,Ohlendorf DH,Earhart CA

更新日期：2007-12-01 00:00:00

abstract：:Mycobacterium tuberculosis utilizes the methylerythritol phosphate (MEP) pathway for biosynthesis of isopentenyl diphosphate and its isomer, dimethylallyl diphosphate, precursors of all isoprenoid compounds. This pathway is of interest as a source of new drug targets, as it is absent from humans and disruption of the ...

journal_title：Journal of bacteriology

authors： Eoh H,Brown AC,Buetow L,Hunter WN,Parish T,Kaur D,Brennan PJ,Crick DC

更新日期：2007-12-01 00:00:00

abstract：:Calcium-dependent exonuclease activity was produced by sporulating cells of Bacillus subtilis 168. Nuclease activity was released into the culture medium at approximately the same time as sporulation proteases, and production of these enzymes was tied to DNA replication. Results suggest that nuclease production is a f...

journal_title：Journal of bacteriology

authors： Burke WF Jr,Slinker BS

更新日期：1982-02-01 00:00:00

abstract：:The cell wall constituents of Leuconostoc citrovorum 8082, L. mesenteroides 10830a, and L. mesenteroides 11449 have been ascertained. All three strains contained glycerol. Glucose and rhamnose were the major reducing sugar constituents. Alanine, glutamic acid, lysine, glucosamine, and muramic acid were the principal a...

journal_title：Journal of bacteriology

authors： Harney SJ,Simopoulos ND,Ikawa M

更新日期：1967-01-01 00:00:00

abstract：:The reversion behavior of pleiotropic carbohydrate mutants, previously designated as ctr, was studied. The mutants revert to complete restoration of the wild-type phenotype, as well as to a spectrum of partial wild-type phenotypes. Lac(+) reversions were found in the lac region (11 min) and some Mal(+) reversions occu...

journal_title：Journal of bacteriology

authors： Wang RJ,Morse HG,Morse ML

更新日期：1970-12-01 00:00:00

abstract：:The developmentally regulated gene dofA, identified from pulse-labeling experiments by two-dimensional gel electrophoresis, and its homologue, dofB, were cloned and characterized in Myxococcus xanthus. Deletion of dofA and dofB did not affect the vegetative growth and development of M. xanthus. dofA was specifically e...

journal_title：Journal of bacteriology

authors： Horiuchi T,Akiyama T,Inouye S,Komano T

更新日期：2002-12-01 00:00:00