Structure and biological activities of beta toxin from Staphylococcus aureus.

Abstract:

:Beta toxin is a neutral sphingomyelinase secreted by certain strains of Staphylococcus aureus. This virulence factor lyses erythrocytes in order to evade the host immune system as well as scavenge nutrients. The structure of beta toxin was determined at 2.4-A resolution using crystals that were merohedrally twinned. This structure is similar to that of the sphingomyelinases of Listeria ivanovii and Bacillus cereus. Beta toxin belongs to the DNase I folding superfamily; in addition to sphingomyelinases, the proteins most structurally related to beta toxin include human endonuclease HAP1, Escherichia coli endonuclease III, bovine pancreatic DNase I, and the endonuclease domain of TRAS1 from Bombyx mori. Our biological assays demonstrated for the first time that beta toxin kills proliferating human lymphocytes. Structure-directed active site mutations show that biological activities, including hemolysis and lymphotoxicity, are due to the sphingomyelinase activity of the enzyme.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Huseby M,Shi K,Brown CK,Digre J,Mengistu F,Seo KS,Bohach GA,Schlievert PM,Ohlendorf DH,Earhart CA

doi

10.1128/JB.00741-07

subject

Has Abstract

pub_date

2007-12-01 00:00:00

pages

8719-26

issue

23

eissn

0021-9193

issn

1098-5530

pii

JB.00741-07

journal_volume

189

pub_type

杂志文章
  • Disruption of a rhodaneselike gene results in cysteine auxotrophy in Saccharopolyspora erythraea.

    abstract::A 3,373-base-pair DNA segment from a clone fortuitously isolated from Saccharopolyspora erythraea by hybridization to an oligodeoxynucleotide probe was sequenced. Computer-assisted analysis of the nucleotide sequence reveals three closely linked Streptomyces open reading frames plus a fourth converging on the others. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.1.350-360.1990

    authors: Donadio S,Shafiee A,Hutchinson CR

    更新日期:1990-01-01 00:00:00

  • Analysis of Halobacterium halobium gas vesicles.

    abstract::Gas vesicles, isolated from lysed Halobacterium halobium cells, gave an amino acid analysis which accounted for 78% of the weight, and the balance was mainly salt and water. One percent of tightly bound d-galactose was found, as well as 2% of phosphate that was not released by treatment which promotes beta-elimination...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.114.3.1058-1067.1973

    authors: Krantz MJ,Ballou CE

    更新日期:1973-06-01 00:00:00

  • Influence of genes encoding proton-translocating enzymes on suppression of Salmonella typhimurium growth and colonization.

    abstract::Twenty-four-hour-old, aerobically grown, Luria-Bertani broth cultures of Salmonella typhimurium F98 suppressed the growth of a spectinomycin-resistant (Spcr) derivative of the same strain inoculated at 10(3) CFU ml(-1). This growth suppression is genus specific and RpoS independent, and it is not solely a result of nu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.22.7186-7190.1997

    authors: Zhang-Barber L,Turner AK,Martin G,Frankel G,Dougan G,Barrow PA

    更新日期:1997-11-01 00:00:00

  • Nitrogenase activity in cell-free extracts of the blue-green alga, Anabaena cylindrica.

    abstract::Cell-free extracts with high nitrogenase activity were prepared by sonic oscillation and French press treatment from the blue-gree alga Anabaena cylindrica. Extracts were prepared from cells grown on a 95% N(2)-5% CO(2) gas mixture followed by a period of nitrogen starvation under an atmosphere of 95% argon-5% CO(2). ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.105.3.913-917.1971

    authors: Smith RV,Evans MC

    更新日期:1971-03-01 00:00:00

  • Methyl ester of hyaluronate is unable to stimulate exolipase formation by Serratia marcescens.

    abstract::Hyaluronate stimulated the formation of exolipase by Serratia marcescens. This ability was abolished when all carboxyl groups of hyaluronate were methyl esterified. Additional studies suggested that the biological inactivity of esterified hyaluronate should be ascribed to the reduced conformational order of the molecu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.139.3.1065-1067.1979

    authors: Jäger KE,Winkler UK

    更新日期:1979-09-01 00:00:00

  • Conservative duplication of spindle poles during meiosis in Saccharomyces cerevisiae.

    abstract::During sporulation in diploid Saccharomyces cerevisiae, spindle pole bodies acquire the so-called meiotic plaque, a prerequisite for spore formation. Mpc70p is a component of the meiotic plaque and is thus essential for spore formation. We show here that MPC70/mpc70 heterozygous strains most often produce two spores i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.7.2372-2375.2001

    authors: Wesp A,Prinz S,Fink GR

    更新日期:2001-04-01 00:00:00

  • Population studies of methicillin-resistant and -sensitive Staphylococcus aureus strains reveal a lack of variability in the agrD gene, encoding a staphylococcal autoinducer peptide.

    abstract::The virulence of Staphylococcus aureus is controlled by the accessory gene regulator (agr) system, including an extracellular inducer encoded by agrD. Variable agr PCR restriction fragment length polymorphism (RFLP) patterns of unique S. aureus strains (n = 192) were determined for a region comprising agrD and parts o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.20.5721-5729.2000

    authors: van Leeuwen W,van Nieuwenhuizen W,Gijzen C,Verbrugh H,van Belkum A

    更新日期:2000-10-01 00:00:00

  • Complete genome sequence of Streptococcus equi subsp. zooepidemicus strain ATCC 35246.

    abstract::Streptococcus equi subsp. zooepidemicus is an opportunistic pathogen. It has caused a very large economic loss in the swine industry of China and has become a threat to human health. We announce the complete genome sequence of S. equi subsp. zooepidemicus strain ATCC 35246, which provides opportunities to understand i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05700-11

    authors: Ma Z,Geng J,Zhang H,Yu H,Yi L,Lei M,Lu CP,Fan HJ,Hu S

    更新日期:2011-10-01 00:00:00

  • Autotrophic and heterotrophic metabolism of Hydrogenomonas. I. Growth yields and patterns under dual substrate conditions.

    abstract::Auxotrophic mutants of Hydrogenomonas eutropha and H. facilis requiring utilizable amino acids were employed to demonstrate the simultaneous utilization of H(2) and an organic substrate for growth. The ratio of the cell yields under dual substrate conditions compared to heterotrophic conditions indicated the relative ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.4.1469-1475.1968

    authors: DeCicco BT,Stukus PE

    更新日期:1968-04-01 00:00:00

  • Growth inhibition and loss of virulence in cultures of Agrobacterium tumefaciens treated with acetosyringone.

    abstract::Acetosyringone, a phenolic inducer of the virulence (vir) genes of Agrobacterium tumefaciens, inhibited the growth of the nopaline-type strains T37 and C58 incubated under acidic conditions. In the course of a 6-day incubation with acetosyringone, avirulent clones were produced in different proportions by strains T37 ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.17.5676-5685.1992

    authors: Fortin C,Nester EW,Dion P

    更新日期:1992-09-01 00:00:00

  • Actin-binding proteins from Burkholderia mallei and Burkholderia thailandensis can functionally compensate for the actin-based motility defect of a Burkholderia pseudomallei bimA mutant.

    abstract::Recently we identified a bacterial factor (BimA) required for actin-based motility of Burkholderia pseudomallei. Here we report that Burkholderia mallei and Burkholderia thailandensis are capable of actin-based motility in J774.2 cells and that BimA homologs of these bacteria can restore the actin-based motility defec...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.22.7857-7862.2005

    authors: Stevens JM,Ulrich RL,Taylor LA,Wood MW,Deshazer D,Stevens MP,Galyov EE

    更新日期:2005-11-01 00:00:00

  • Regions of Salmonella typhimurium flagellin essential for its polymerization and excretion.

    abstract::Immunological methods were used to examine the flagellin production of Salmonella typhimurium strains that carried a mutation in one of the two possible genes for flagellin (H1 or H2) and also were incapable of expressing the other gene. Some mutants produced flagellin that was excreted into the culture medium; others...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.1.291-296.1987

    authors: Homma M,Fujita H,Yamaguchi S,Iino T

    更新日期:1987-01-01 00:00:00

  • Role of uracil-DNA glycosylase in mutation avoidance by Streptococcus pneumoniae.

    abstract::Uracil-DNA glycosylase activity was found in Streptococcus pneumoniae, and the enzyme was partially purified. An ung mutant lacking the activity was obtained by positive selection of cells transformed with a plasmid containing uracil in its DNA. The effects of the ung mutation on mutagenic processes in S. pneumoniae w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.1.283-290.1991

    authors: Chen JD,Lacks SA

    更新日期:1991-01-01 00:00:00

  • Nucleotide sequence and transcriptional analysis of the Escherichia coli agp gene encoding periplasmic acid glucose-1-phosphatase.

    abstract::The nucleotide sequence of the agp gene, which encodes a periplasmic glucose-1-phosphatase, was determined. The deduced amino acid sequence corresponds to a 413-amino-acid-residue polypeptide with a typical hydrophobic signal sequence of 22 amino acids. The mature protein lacks the N-terminal signal peptide and has a ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.2.802-807.1990

    authors: Pradel E,Marck C,Boquet PL

    更新日期:1990-02-01 00:00:00

  • Identification and sequence analysis of the Rhizobium meliloti dctA gene encoding the C4-dicarboxylate carrier.

    abstract::Transposon Tn5-induced C4-dicarboxylate transport mutants of Rhizobium meliloti 2011 which could be complemented by cosmid pRmSC121 were subdivided into two classes. Class I mutants (RMS37 and RMS938) were defective in symbiotic C4-dicarboxylate transport and in nitrogen fixation. They were mutated in the structural g...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.10.5551-5560.1989

    authors: Engelke T,Jording D,Kapp D,Pühler A

    更新日期:1989-10-01 00:00:00

  • Monoubiquitination is sufficient to signal internalization of the maltose transporter in Saccharomyces cerevisiae.

    abstract::Monoubiquitination of the 12-transmembrane segment (12-TMS) Saccharomyces cerevisiae maltose transporter promoted the maximal internalization rate of this protein. This modification is similar to that of the 7-TMS alpha-factor receptor but different from that of the 12-TMS uracil and general amino acid permeases. This...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.1.241-243.2000

    authors: Lucero P,Peñalver E,Vela L,Lagunas R

    更新日期:2000-01-01 00:00:00

  • A subassembly of R27-encoded transfer proteins is dependent on TrhC nucleoside triphosphate-binding motifs for function but not formation.

    abstract::The transfer of plasmid DNA molecules between bacterial cells is achieved by a large array of conjugative transfer proteins which assemble into both cytoplasmic and membrane-associated complexes. TrhC is a membrane-associated protein that is required for the transfer of the IncHI1 resistance plasmid R27. Homologous pr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.6.1606-1613.2004

    authors: Gilmour MW,Taylor DE

    更新日期:2004-03-01 00:00:00

  • Identification of an Escherichia coli operon required for formation of the O-antigen capsule.

    abstract::Escherichia coli produces polysaccharide capsules that, based on their mechanisms of synthesis and assembly, have been classified into four groups. The group 4 capsule (G4C) polysaccharide is frequently identical to that of the cognate lipopolysaccharide O side chain and has, therefore, also been termed the O-antigen ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.15.5259-5266.2005

    authors: Peleg A,Shifrin Y,Ilan O,Nadler-Yona C,Nov S,Koby S,Baruch K,Altuvia S,Elgrably-Weiss M,Abe CM,Knutton S,Saper MA,Rosenshine I

    更新日期:2005-08-01 00:00:00

  • Construction of an Enterococcus faecalis Tn917-mediated-gene-disruption library offers insight into Tn917 insertion patterns.

    abstract::Sequencing the insertion sites of 8,865 Tn917 insertions in Enterococcus faecalis strain OG1RF identified a hot spot in the replication terminus region corresponding to 6% of the genome where 65% of the transposons had inserted. In E. faecalis, Tn917 preferentially inserted at a 29-bp consensus sequence centered on TA...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.21.7280-7289.2004

    authors: Garsin DA,Urbach J,Huguet-Tapia JC,Peters JE,Ausubel FM

    更新日期:2004-11-01 00:00:00

  • Mutation in Pseudomonas aeruginosa causing simultaneous defects in penicillin-binding protein 5 and in enzyme activities of penicillin release and D-alanine carboxypeptidase.

    abstract::Penicillin-binding protein 5 in Pseudomonas aeruginosa had moderately penicillin-sensitive D-alanine carboxypeptidase activity. As in Escherichia coli, a defect in this enzyme activity was not lethal. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.2.849-851.1985

    authors: Noguchi H,Fukasawa M,Komatsu T,Mitsuhashi S,Matsuhashi M

    更新日期:1985-05-01 00:00:00

  • Chemoreceptors and flagellar motors are subterminally located in close proximity at the two cell poles in spirochetes.

    abstract::Green fluorescent protein (GFP) fusions, immunofluorescence microscopy, and cryo-electron tomography revealed that the chemoreceptors of the Lyme disease spirochete Borrelia burgdorferi form long, thin arrays near both cell poles. These arrays are in close proximity to the flagellar motors. This information provides a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01530-10

    authors: Xu H,Raddi G,Liu J,Charon NW,Li C

    更新日期:2011-05-01 00:00:00

  • Cell shape and division in Escherichia coli: experiments with shape and division mutants.

    abstract::Double mutants which carry mutations in genes (rodA, pbpA) required for cell elongation (i.e., maintenance of rod shape) in combination with mutations in genes (ftsA, ftsI, ftsQ, or ftsZ) required for septation were constructed. Such mutants were able to grow for about two mass doublings at a normal rate at the restri...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.163.2.615-622.1985

    authors: Begg KJ,Donachie WD

    更新日期:1985-08-01 00:00:00

  • Comprehensive Spatial Analysis of the Borrelia burgdorferi Lipoproteome Reveals a Compartmentalization Bias toward the Bacterial Surface.

    abstract::The Lyme disease spirochete Borrelia burgdorferi is unique among bacteria in its large number of lipoproteins that are encoded by a small, exceptionally fragmented, and predominantly linear genome. Peripherally anchored in either the inner or outer membrane and facing either the periplasm or the external environment, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00658-16

    authors: Dowdell AS,Murphy MD,Azodi C,Swanson SK,Florens L,Chen S,Zückert WR

    更新日期:2017-02-28 00:00:00

  • Medium-dependent variation of deoxyribonucleic acid segregation in Escherichia coli.

    abstract::The degree to which deoxyribonucleic acid segregates nonrandomly has been investigated for Escherichia coli B/r growing in different media. The degree of nonrandom segregation observed is dependent on the medium, with segregation becoming less random as the growth rate decreases. This indicates that there must be some...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.130.1.118-127.1977

    authors: Cooper S,Weinberger M

    更新日期:1977-04-01 00:00:00

  • The periplasmic nitrate reductase nap is required for anaerobic growth and involved in redox control of magnetite biomineralization in Magnetospirillum gryphiswaldense.

    abstract::The magnetosomes of many magnetotactic bacteria consist of membrane-enveloped magnetite crystals, whose synthesis is favored by a low redox potential. However, the cellular redox processes governing the biomineralization of the mixed-valence iron oxide have remained unknown. Here, we show that in the alphaproteobacter...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00903-12

    authors: Li Y,Katzmann E,Borg S,Schüler D

    更新日期:2012-09-01 00:00:00

  • pdc1(0) mutants of Saccharomyces cerevisiae give evidence for an additional structural PDC gene: cloning of PDC5, a gene homologous to PDC1.

    abstract::The PDC1 gene coding for a pyruvate decarboxylase (PDC; EC 4.1.1.1) was deleted from the Saccharomyces cerevisiae genome. The resulting pdc1(0) mutants were able to grow on glucose and still contained 60 to 70% of the wild-type PDC activity. Two DNA fragments with sequences homologous to that of the PDC1 gene were clo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.2.678-685.1990

    authors: Seeboth PG,Bohnsack K,Hollenberg CP

    更新日期:1990-02-01 00:00:00

  • Complete genome sequence of the type strain Cupriavidus necator N-1.

    abstract::Here we announce the complete genome sequence of the copper-resistant bacterium Cupriavidus necator N-1, the type strain of the genus Cupriavidus. The genome consists of two chromosomes and two circular plasmids. Based on genome comparison, the chromosomes of C. necator N-1 share a high degree of similarity with the t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05660-11

    authors: Poehlein A,Kusian B,Friedrich B,Daniel R,Bowien B

    更新日期:2011-09-01 00:00:00

  • Exfoliatin-producing strains define a fourth agr specificity group in Staphylococcus aureus.

    abstract::The staphylococcal virulon is activated by the density-sensing agr system, which is autoinduced by a short peptide (autoinducing peptide [AIP]) processed from a propeptide encoded by agrD. A central segment of the agr locus, consisting of the C-terminal two-thirds of AgrB (the putative processing enzyme), AgrD, and th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.22.6517-6522.2000

    authors: Jarraud S,Lyon GJ,Figueiredo AM,Lina G,Vandenesch F,Etienne J,Muir TW,Novick RP

    更新日期:2000-11-01 00:00:00

  • Methods for the determination of deoxyribonucleic Acid homologies in streptomyces.

    abstract::Variations of the membrane filter technique for deoxyribonucleic acid (DNA) hybridizations were studied with respect to Streptomyces species. At the temperatures required for specific hybridization of DNA with the high melting temperature (T(m)) characteristic of Streptomyces, large amounts (up to 97%) of filter-bound...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.3.1086-1094.1970

    authors: Okanishi M,Gregory KF

    更新日期:1970-12-01 00:00:00

  • Effect of temperature on the distribution of membrane particles in Streptococcus faecalis as seen by the freeze-fracture technique.

    abstract::When cells of Streptococcus faecalis ATCC 9790 were incubated at temperatures above 10 C before being frozen for freeze-fracture, a random distribution of particles was observed on the outer fracture face of the freeze-cleaved cell membrane. However, when cells were incubated below 10 C before freezing, particleless p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.118.2.725-734.1974

    authors: Tsien HC,Higgins ML

    更新日期:1974-05-01 00:00:00