New recA mutations that dissociate the various RecA protein activities in Escherichia coli provide evidence for an additional role for RecA protein in UV mutagenesis.


:To isolate strains with new recA mutations that differentially affect RecA protein functions, we mutagenized in vitro the recA gene carried by plasmid mini-F and then introduced the mini-F-recA plasmid into a delta recA host that was lysogenic for prophage phi 80 and carried a lac duplication. By scoring prophage induction and recombination of the lac duplication, we isolated new recA mutations. A strain carrying mutation recA1734 (Arg-243 changed to Leu) was found to be deficient in phi 80 induction but proficient in recombination. The mutation rendered the host not mutable by UV, even in a lexA(Def) background. Yet, the recA1734 host became mutable upon introduction of a plasmid encoding UmuD*, the active carboxyl-terminal fragment of UmuD. Although the recA1734 mutation permits cleavage of lambda and LexA repressors, it renders the host deficient in the cleavage of phi 80 repressor and UmuD protein. Another strain carrying mutation recA1730 (Ser-117 changed to Phe) was found to be proficient in phi 80 induction but deficient in recombination. The recombination defect conferred by the mutation was partly alleviated in a cell devoid of LexA repressor, suggesting that, when amplified, RecA1730 protein is active in recombination. Since LexA protein was poorly cleaved in the recA1730 strain while phage lambda was induced, we conclude that RecA1730 protein cannot specifically mediate LexA protein cleavage. Our results show that the recA1734 and recA1730 mutations differentially affect cleavage of various substrates. The recA1730 mutation prevented UV mutagenesis, even upon introduction into the host of a plasmid encoding UmuD* and was dominant over recA+. With respect to other RecA functions, recA1730 was recessive to recA+. This demonstrates that RecA protein has an additional role in mutagenesis beside mediating the cleavage of LexA and UmuD proteins.


J Bacteriol


Journal of bacteriology


Dutreix M,Moreau PL,Bailone A,Galibert F,Battista JR,Walker GC,Devoret R




Has Abstract


1989-05-01 00:00:00












  • Oxidative metabolism in Pediococcus pentosaceus. II. Factors controlling the formation of oxidative activities.

    abstract::Dobrogosz, Walter J. (The Pennsylvania State University, University Park) and Robert W. Stone. Oxidative metabolism in Pediococcus pentosaceus. II. Factors controlling the formation of oxidative activities. J. Bacteriol. 84:724-729. 1962.-Studies were conducted on some factors which regulate the formation of oxidative...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章



    更新日期:1962-10-01 00:00:00

  • Characterization and use of catabolite-repressed promoters from gluconate genes in Corynebacterium glutamicum.

    abstract::The genes involved in gluconate catabolism (gntP and gntK) in Corynebacterium glutamicum are scattered in the chromosome, and no regulatory genes are apparently associated with them, in contrast with the organization of the gnt operon in Escherichia coli and Bacillus subtilis. In C. glutamicum, gntP and gntK are essen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Letek M,Valbuena N,Ramos A,Ordóñez E,Gil JA,Mateos LM

    更新日期:2006-01-01 00:00:00

  • Ultraviolet-sensitive mutator strain of Escherichia coli K-12.

    abstract::An ultraviolet (UV)-sensitive mutator gene, mutU, was identified in Escherichia coli K-12. The mutation mutU4 is very close to uvrD, between metE and ilv, on the E. coli chromosome. It was recessive as a mutator and as a UV-sensitive mutation. The frequency of reversion of trpA46 on an F episome was increased by mutU4...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Siegel EC

    更新日期:1973-01-01 00:00:00

  • Purification of flagellar cores of Vibrio cholerae.

    abstract::A procedure is described for the purification of the cores of flagella sheared from Vibrio cholerae. V. cholerae is a monotrichous organism whose flagellar core (FC) is enclosed within a sheath. The purification procedure consists of several cycles of differential centrifugation and cesium chloride density-gradient ul...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Yang GC,Schrank GD,Freeman BA

    更新日期:1977-02-01 00:00:00

  • Loss of the penicillinase plasmid after treatment of Staphylococcus aureus with sodium dodecyl sulfate.

    abstract::Two strains of Staphylococcus aureus (PC1 and 196E), when grown in medium containing 0.002% sodium dodecyl sulfate (SDS), showed from 96.1 to 100% loss of the ability to produce penicillinase. Resistance to cadmium and zinc was lost concomitantly with the ability to produce penicillinase. A comparison of the rate of c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sonstein SA,Baldwin JN

    更新日期:1972-01-01 00:00:00

  • Genetic and structural organization of the aminophenol catabolic operon and its implication for evolutionary process.

    abstract::The aminophenol (AP) catabolic operon in Pseudomonas putida HS12 mineralizing nitrobenzene was found to contain all the enzymes responsible for the conversion of AP to pyruvate and acetyl coenzyme A via extradiol meta cleavage of 2-aminophenol. The sequence and functional analyses of the corresponding genes of the ope...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Park HS,Kim HS

    更新日期:2001-09-01 00:00:00

  • Enterohemolysin production is associated with a temperate bacteriophage in Escherichia coli serogroup O26 strains.

    abstract::A temperate bacteriophage that determines the expression of enterohemolysin was isolated from Escherichia coli O26 strain C3888. The genetic determinant associated with enterohemolysin production (E-Hly determinant) was cloned from EcoRI-digested bacteriophage DNA in vector plasmid pUC8. pUC8 recombinant plasmid pEO19...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Beutin L,Bode L,Ozel M,Stephan R

    更新日期:1990-11-01 00:00:00

  • Nitric oxide signaling in Pseudomonas aeruginosa biofilms mediates phosphodiesterase activity, decreased cyclic di-GMP levels, and enhanced dispersal.

    abstract::Bacteria in biofilms often undergo active dispersal events and revert to a free-swimming, planktonic state to complete the biofilm life cycle. The signaling molecule nitric oxide (NO) was previously found to trigger biofilm dispersal in the opportunistic pathogen Pseudomonas aeruginosa at low, nontoxic concentrations ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Barraud N,Schleheck D,Klebensberger J,Webb JS,Hassett DJ,Rice SA,Kjelleberg S

    更新日期:2009-12-01 00:00:00

  • Adenosine 5'-triphosphate synthesis energized by an artificially imposed membrane potential in membrane vesicles of Escherichia coli.

    abstract::Adenosine 5'-triphosphate (ATP) synthesis driven by an artificially imposed membrane potential in right-side-out membrane vesicles of Escherichia coli was investigated. Membrane vesicles prepared in the presence of adenosine diphosphate were loaded with K+ by incubation with 0.5 M potassium phosphate. Addition of vali...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Tsuchiya T,Rosen BP

    更新日期:1976-07-01 00:00:00

  • The pi-histidine factor of Salmonella typhimurium: a demonstration that pi-histidine factor integrates into the chromosome.

    abstract::The Salmonella typhimurium pi-histidine episome was identified by Ames et al. (2) in an unstable partial revertant of a deletion mutation-containing strain, hisG203. HisG203 lacks the histidine operator, promoter, and part of the first structural gene. In this paper, we study some properties of pi factor and demonstra...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Levinthal M,Yeh J

    更新日期:1972-03-01 00:00:00

  • Isolation and characterization of an Escherichia coli seryl-tRNA synthetase mutant with a large increase in Km for serine.

    abstract::A mutant of Escherichia coli resistant to serine hydroxamate which has a large increase in Km for serine of seryl-tRNA synthetase is described. The mutant serS gene was cloned and sequenced and was found to contain a single-base-pair mutation, resulting in the substitution of the residue alanine 262 by valine in motif...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Willison JC,Härtlein M,Leberman R

    更新日期:1995-06-01 00:00:00

  • Metabolism of biphenyl by Aspergillus toxicarius: induction of hydroxylating activity and accumulation of water-soluble conjugates.

    abstract::Biphenyl metabolism in Aspergillus toxicarius occurs by successive hydroxylations in the 4- and 4'-positions, followed by conjugation with sulfate to produce 4-hydroxybiphenyl-O-sulfonic acid and 4,4'-dihydroxybiphenyl-O-sulfonic acid. The hydroxylation reactions normally occur only after a prolonged lag period after ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Golbeck JH,Albaugh SA,Radmer R

    更新日期:1983-10-01 00:00:00

  • Identification of a complex operator for galP1, the glucose-sensitive, galactose-dependent promoter of the Streptomyces galactose operon.

    abstract::The galP1 promoter is responsible for galactose-dependent, glucose-sensitive transcription of the galactose utilization operon of Streptomyces coelicolor and Streptomyces lividans. We describe the characterization of mutations that were positioned directly upstream of the apparent transcription start site of galP1 and...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Mattern SG,Brawner ME,Westpheling J

    更新日期:1993-03-01 00:00:00

  • Two different dihydroorotate dehydrogenases in Lactococcus lactis.

    abstract::The pyrimidine de novo biosynthesis pathway has been characterized for a number of organisms. The general pathway consists of six enzymatic steps. In the characterization of the pyrimidine pathway of Lactococcus lactis, two different pyrD genes encoding dihydroorotate dehydrogenase were isolated. The nucleotide sequen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Andersen PS,Jansen PJ,Hammer K

    更新日期:1994-07-01 00:00:00

  • Inactivation of Serpulina hyodysenteriae flaA1 and flaB1 periplasmic flagellar genes by electroporation-mediated allelic exchange.

    abstract::Serpulina hyodysenteriae, the etiologic agent of swine dysentery, contains complex periplasmic flagella which are composed of multiple class A and class B polypeptides. To examine the role these proteins play in flagellar synthesis, structure, and function and to develop strains which may provide insight into the impo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Rosey EL,Kennedy MJ,Petrella DK,Ulrich RG,Yancey RJ Jr

    更新日期:1995-10-01 00:00:00

  • AGT1, encoding an alpha-glucoside transporter involved in uptake and intracellular accumulation of trehalose in Saccharomyces cerevisiae.

    abstract::The trehalose content in Saccharomyces cerevisiae can be significantly manipulated by including trehalose at an appropriate level in the growth medium. Its uptake is largely dependent on the expression of AGT1, which encodes an alpha-glucoside transporter. The trehalose found in a tps1 mutant of trehalose synthase may...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Plourde-Owobi L,Durner S,Parrou JL,Wieczorke R,Goma G,François J

    更新日期:1999-06-01 00:00:00

  • Occurrence of isocitrate lyase in a thermophilic bacillus species.

    abstract::A thermophilic, sporeforming bacterium has been isolated from soil on a medium containing acetate as a carbon source. This organism is similar to Bacillus stearothermophilus in most respects but differs in its inability to hydrolyze starch. Isocitrate lyase is present in cell-free extracts of organisms grown in a medi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Daron HH

    更新日期:1967-02-01 00:00:00

  • Cyclic guanosine 3',5'-monophosphate in the dimorphic fungus Mucor racemosus.

    abstract::The dimorphic fungus Mucor racemosus was found to contain the cyclic nucleotide guanosine 3',5'-monophosphate (cGMP). Approximately equivalent amounts of the compound were found in ungerminated spores, yeastlike cells, and mycelia. Germinating spores contained severalfold higher amounts of cGMP than the other cell for...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Orlowski M,Sypherd PS

    更新日期:1976-03-01 00:00:00

  • The developmentally regulated alb1 gene of Aspergillus fumigatus: its role in modulation of conidial morphology and virulence.

    abstract::Aspergillus fumigatus, an important opportunistic pathogen which commonly affects neutropenic patients, produces conidia with a bluish-green color. We identified a gene, alb1, which is required for conidial pigmentation. The alb1 gene encodes a putative polyketide synthase, and disruption of alb1 resulted in an albino...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Tsai HF,Chang YC,Washburn RG,Wheeler MH,Kwon-Chung KJ

    更新日期:1998-06-01 00:00:00

  • Carbohydrate Accumulation and Metabolism in Escherichia coli: Characteristics of the Reversions of ctr Mutations.

    abstract::The reversion behavior of pleiotropic carbohydrate mutants, previously designated as ctr, was studied. The mutants revert to complete restoration of the wild-type phenotype, as well as to a spectrum of partial wild-type phenotypes. Lac(+) reversions were found in the lac region (11 min) and some Mal(+) reversions occu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Wang RJ,Morse HG,Morse ML

    更新日期:1970-12-01 00:00:00

  • Transcriptional regulation of the heat shock regulatory gene rpoH in Escherichia coli: involvement of a novel catabolite-sensitive promoter.

    abstract::A catabolite-sensitive promoter was found to be involved in transcription of the heat shock regulatory gene rpoH encoding the sigma 32 protein. Expression of lacZ from the operon fusion, rpoHp-lacZ, was partially inhibited by glucose added to the broth medium. Dissection of the rpoH promoter region allowed us to local...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Nagai H,Yano R,Erickson JW,Yura T

    更新日期:1990-05-01 00:00:00

  • Functional genomics enables identification of genes of the arginine transaminase pathway in Pseudomonas aeruginosa.

    abstract::Arginine utilization in Pseudomonas aeruginosa with multiple catabolic pathways represents one of the best examples of the metabolic versatility of this organism. To identify genes involved in arginine catabolism, we have employed DNA microarrays to analyze the transcriptional profiles of this organism in response to ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Yang Z,Lu CD

    更新日期:2007-06-01 00:00:00

  • Evidence for the Translocation of a Chromosome Sement in Bacillus subtilis Strains Carrying the trpE26 Mutation.

    abstract::The replication order of markers was studied in Bacillus subtilis strains bearing the trpE26 mutation by the use of the density transfer technique. An important difference in this order was observed in comparison with that of strain 168 T-. All markers tested of a chromosome segment extending from trpD to ilvA replica...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Trowsdale J,Anagnostopoulos C

    更新日期:1975-06-01 00:00:00

  • Influence of genes encoding proton-translocating enzymes on suppression of Salmonella typhimurium growth and colonization.

    abstract::Twenty-four-hour-old, aerobically grown, Luria-Bertani broth cultures of Salmonella typhimurium F98 suppressed the growth of a spectinomycin-resistant (Spcr) derivative of the same strain inoculated at 10(3) CFU ml(-1). This growth suppression is genus specific and RpoS independent, and it is not solely a result of nu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Zhang-Barber L,Turner AK,Martin G,Frankel G,Dougan G,Barrow PA

    更新日期:1997-11-01 00:00:00

  • Metabolism of D-arabinose by Aerobacter aerogenes: purification of the isomerase.

    abstract::In Aerobacter aerogenes, the mutational event permitting the utilization of d-arabinose as a source of carbon and energy is a regulatory mutation resulting in the constitutive synthesis of certain enzymes of the l-fucose catabolic pathway. l-Fucose isomerase catalyzes the isomerization of d-arabinose to d-ribulose. Th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Oliver EJ,Mortlock RP

    更新日期:1971-10-01 00:00:00

  • The promoter for a sporulation gene in the spoIVC locus of Bacillus subtilis and its use in studies of temporal and spatial control of gene expression.

    abstract::We have identified the transcription start site and regulatory region governing the expression of a sporulation gene in the spoIVC locus of Bacillus subtilis. Efficient expression and developmental regulation of this gene was controlled from a promoter region that extended no more than 110 base pairs upstream and no m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kunkel B,Sandman K,Panzer S,Youngman P,Losick R

    更新日期:1988-08-01 00:00:00

  • Subcellular localization and immunological detection of proteins encoded by the vir locus of Bordetella pertussis.

    abstract::The DNA sequence of the central regulatory locus vir of Bordetella pertussis predicts that three gene products, BvgA, BvgB, and BvgC, are encoded. Features of the predicted primary structures of these proteins and their homology to other two-component systems suggest that BvgA is located in the cytoplasm, BvgB is loca...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Stibitz S,Yang MS

    更新日期:1991-07-01 00:00:00

  • A multifunction ABC transporter (Opt) contributes to diversity of peptide uptake specificity within the genus Lactococcus.

    abstract::Growth of Lactococcus lactis in milk depends on the utilization of extracellular peptides. Up to now, oligopeptide uptake was thought to be due only to the ABC transporter Opp. Nevertheless, analysis of several Opp-deficient L. lactis strains revealed the implication of a second oligopeptide ABC transporter, the so-ca...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lamarque M,Charbonnel P,Aubel D,Piard JC,Atlan D,Juillard V

    更新日期:2004-10-01 00:00:00

  • Repair and antirepair DNA helicases in Helicobacter pylori.

    abstract::Orthologs of RecG and RuvABC are highly conserved among prokaryotes; in Escherichia coli, they participate in independent pathways that branch migrate Holliday junctions during recombinational DNA repair. RecG also has been shown to directly convert stalled replication forks into Holliday junctions. The bacterium Heli...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kang J,Blaser MJ

    更新日期:2008-06-01 00:00:00

  • Identification and Characterization of a New 7-Aminocephalosporanic Acid Deacetylase from Thermophilic Bacterium Alicyclobacillus tengchongensis.

    abstract:UNLABELLED:Deacetylation of 7-aminocephalosporanic acid (7-ACA) at position C-3 provides valuable starting material for producing semisynthetic β-lactam antibiotics. However, few enzymes have been characterized in this process before now. Comparative analysis of the genome of the thermophilic bacterium Alicyclobacillus...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ding JM,Yu TT,Han NY,Yu JL,Li JJ,Yang YJ,Tang XH,Xu B,Zhou JP,Tang HZ,Huang ZX

    更新日期:2015-11-02 00:00:00