Chromosome map of Xanthomonas campestris pv. campestris 17 with locations of genes involved in xanthan gum synthesis and yellow pigmentation.

Abstract:

:No plasmid was detected in Xanthomonas campestris pv. campestris 17, a strain of the causative agent of black rot in cruciferous plants isolated in Taiwan. Its chromosome was cut by PacI, PmeI, and SwaI into five, two, and six fragments, respectively, and a size of 4.8 Mb was estimated by summing the fragment lengths in these digests. Based on the data obtained from partial digestion and Southern hybridization using probes common to pairs of the overlapping fragments or prepared from linking fragments, a circular physical map bearing the PacI, PmeI, and SwaI sites was constructed for the X. campestris pv. campestris 17 chromosome. Locations of eight eps loci involved in exopolysaccharide (xanthan gum) synthesis, two rrn operons each possessing an unique I-CeuI site, one pig cluster required for yellow pigmentation, and nine auxotrophic markers were determined, using mutants isolated by mutagenesis with Tn5(pfm)CmKm. This transposon contains a polylinker with sites for several rare-cutting restriction endonucleases located between the chloramphenicol resistance and kanamycin resistance (Kmr) genes, which upon insertion introduced additional sites into the chromosome. The recA and tdh genes, with known sequences, were mapped by tagging with the polylinker-Kmr segment from Tn5(pfm)CmKm. This is the first map for X. campestris and would be useful for genetic studies of this and related Xanthomonas species.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Tseng YH,Choy KT,Hung CH,Lin NT,Liu JY,Lou CH,Yang BY,Wen FS,Weng SF,Wu JR

doi

10.1128/JB.181.1.117-125.1999

subject

Has Abstract

pub_date

1999-01-01 00:00:00

pages

117-25

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

181

pub_type

杂志文章
  • The C-terminal portion of the tail fiber protein of bacteriophage lambda is responsible for binding to LamB, its receptor at the surface of Escherichia coli K-12.

    abstract::Bacteriophage lambda adsorbs to its Escherichia coli K-12 host by interacting with LamB, its cell-surface receptor. We fused C-terminal portions of J, the tail fiber protein of lambda, to maltose-binding protein. Solid-phase binding assays demonstrated that a purified fusion protein comprising only the last 249 residu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.2.508-512.2000

    authors: Wang J,Hofnung M,Charbit A

    更新日期:2000-01-01 00:00:00

  • Bacterial cis-dihydrodiol dehydrogenases: comparison of physicochemical and immunological protperties.

    abstract::Cells of Pseudomonas putida NP, Pseudomonas species (NCIB 9816), and a Nocardia species, after growth on naphthalene as sole source of carbon and energy, contain a nicotinamide adenine dinucleotide (NAD+)-dependent enzyme that oxidizes cis-dihydrodiols of mono- and polycyclic aromatic compounds. Similarly, cells of a ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.128.3.842-850.1976

    authors: Patel TR,Gibson DT

    更新日期:1976-12-01 00:00:00

  • Sortase B, a new class of sortase in Listeria monocytogenes.

    abstract::Sortases are transamidases that covalently link proteins to the peptidoglycan of gram-positive bacteria. The genome of the pathogenic bacterium Listeria monocytogenes encodes two sortases genes, srtA and srtB. The srtA gene product anchors internalin and some other LPXTG-containing proteins to the listerial surface. H...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.7.1972-1982.2004

    authors: Bierne H,Garandeau C,Pucciarelli MG,Sabet C,Newton S,Garcia-del Portillo F,Cossart P,Charbit A

    更新日期:2004-04-01 00:00:00

  • Evolutionary relationship between the TonB-dependent outer membrane transport proteins: nucleotide and amino acid sequences of the Escherichia coli colicin I receptor gene.

    abstract::The nucleotide sequence of the Escherichia coli colicin I receptor gene (cir) has been determined. The predicted mature protein consists of 599 amino acids and has a molecular weight of 67,169. Several previously noted characteristics of other E. coli outer membrane protein sequences were also identified in the sequen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.2.1041-1047.1989

    authors: Nau CD,Konisky J

    更新日期:1989-02-01 00:00:00

  • Roles for the Rhodobacter sphaeroides CcmA and CcmG proteins.

    abstract::Rhodobacter sphaeroides cells containing an in-frame deletion within ccmA lack detectable soluble and membrane-bound c-type cytochromes and are unable to grow under conditions where these proteins are required. Only strains merodiploid for ccmABCDG were found after attempting to generate cells containing either a ccmG...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.15.4643-4647.2001

    authors: Cox RL,Patterson C,Donohue TJ

    更新日期:2001-08-01 00:00:00

  • Cloning of genes coding for the three subunits of thiocyanate hydrolase of Thiobacillus thioparus THI 115 and their evolutionary relationships to nitrile hydratase.

    abstract::Thiocyanate hydrolase is a newly found enzyme from Thiobacillus thioparus THI 115 that converts thiocyanate to carbonyl sulfide and ammonia (Y. Katayama, Y. Narahara, Y. Inoue, F. Amano, T. Kanagawa, and H. Kuraishi, J. Biol. Chem. 267:9170-9175, 1992). We have cloned and sequenced the scn genes that encode the three ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.10.2583-2589.1998

    authors: Katayama Y,Matsushita Y,Kaneko M,Kondo M,Mizuno T,Nyunoya H

    更新日期:1998-05-01 00:00:00

  • Chemical analyses of asporogenic mutants of Bacillus cereus.

    abstract::Lundgren, D. G. (Syracuse University, Syracuse, N.Y.) and J. J. Cooney. Chemical analyses of asporogenic mutants of Bacillus cereus. J. Bacteriol. 83:1287-1293. 1962.-Bacillus cereus ATCC 4342 and three temperature-sensitive asporogenic mutants were compared regarding some basic cellular components and response to met...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.6.1287-1293.1962

    authors: LUNDGREN DG,COONEY JJ

    更新日期:1962-06-01 00:00:00

  • Characterization and virulence properties of Erwinia chrysanthemi lipopolysaccharide-defective, phi EC2-resistant mutants.

    abstract::Outer membrane alterations were characterized in spontaneous mutants of the Erwinia chrysanthemi 3937jRH, which were selected for resistance to bacteriophage phi EC2. All but one of the mutants analyzed were affected in their lipopolysaccharide (LPS) structure, lacking the entire heterogeneous region of apparent high ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.9.4011-4017.1987

    authors: Schoonejans E,Expert D,Toussaint A

    更新日期:1987-09-01 00:00:00

  • Biological factors affecting enflagellation of Naegleria fowleri.

    abstract::Naegleria fowleri is a pathogenic amoeboflagellate that can be evoked to transform from amoebae to flagellates by subculture to nonnutrient buffer. More than half of the amoebae of strains KUL, nN68, and Lovell became enflagellated 300 min after subculture to amoeba-saline, whereas no amoebae of strains NF66, NF69, an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Woodworth TW,John DT,Bradley SG

    更新日期:1982-11-01 00:00:00

  • Influence of iron on yields of extracellular products in Pseudomonas aeruginosa cultures.

    abstract::The effect of the iron content of the medium on the yields of extracellular products by seven distinct strains of Pseudomonas aeruginosa was examined. All strains showed at least an 85% decrease in toxin A yields when grown in medium containing 5.0 mug of iron per ml (high iron) as compared to 0.05 mug/ml (low iron), ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.138.1.193-200.1979

    authors: Bjorn MJ,Sokol PA,Iglewski BH

    更新日期:1979-04-01 00:00:00

  • Estimations of bacterial growth rates in natural waters.

    abstract::Specific growth rates as low as 0.005 hr(-1) (generation times of 20 to 200 hr) of aquatic bacteria in natural waters have been calculated from significant differences between dilution rates and washout rates in a chemostat. The measured growth rates were affected by the treatment of the water samples (type of sterili...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.99.1.156-160.1969

    authors: Jannasch HW

    更新日期:1969-07-01 00:00:00

  • Sulfur amino acid metabolism and its control in Lactococcus lactis IL1403.

    abstract::Cysteine and methionine availability influences many processes in the cell. In bacteria, transcription of the specific genes involved in the synthesis of these two amino acids is usually regulated by different mechanisms or regulators. Pathways for the synthesis of cysteine and methionine and their interconversion wer...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.11.3762-3778.2005

    authors: Sperandio B,Polard P,Ehrlich DS,Renault P,Guédon E

    更新日期:2005-06-01 00:00:00

  • In vitro transcription of the nitrogen fixation regulatory operon nifLA of Klebsiella pneumoniae.

    abstract::In vitro transcription from the promoter for the nitrogen fixation regulatory operon nifLA of K. pneumoniae requires four protein fractions: the core form of RNA polymerase; NTRA, an alternate sigma factor; NTRC, an auxiliary DNA-binding protein; and NTRB, a bifunctional enzyme that controls the activity of NTRC by co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.6.2876-2880.1987

    authors: Wong PK,Popham D,Keener J,Kustu S

    更新日期:1987-06-01 00:00:00

  • The All-Alpha Domains of Coupling Proteins from the Agrobacterium tumefaciens VirB/VirD4 and Enterococcus faecalis pCF10-Encoded Type IV Secretion Systems Confer Specificity to Binding of Cognate DNA Substrates.

    abstract:UNLABELLED:Bacterial type IV coupling proteins (T4CPs) bind and mediate the delivery of DNA substrates through associated type IV secretion systems (T4SSs). T4CPs consist of a transmembrane domain, a conserved nucleotide-binding domain (NBD), and a sequence-variable helical bundle called the all-alpha domain (AAD). In ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00189-15

    authors: Whitaker N,Chen Y,Jakubowski SJ,Sarkar MK,Li F,Christie PJ

    更新日期:2015-07-01 00:00:00

  • Isolation and preliminary characterization of Saccharomyces cerevisiae proline auxotrophs.

    abstract::Proline-requiring mutants of Saccharomyces cerevisiae were isolated. Each mutation is recessive and is inherited as expected for a single nuclear gene. Three complementation groups cold be defined which are believed to correspond to mutations in the three genes (pro1, pro2, and pro3) coding for the three enzymes of th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.138.3.816-822.1979

    authors: Brandriss MC

    更新日期:1979-06-01 00:00:00

  • Interactive regulation of Azorhizobium nifA transcription via overlapping promoters.

    abstract::The Azorhizobium nifA promoter (PnifA) is positively regulated by two physiological signal transduction pathways, NtrBC, which signals anabolic N status, and FixLJK, which signals prevailing O2 status. Yet, PnifA response (gene product per unit time) to these two activating signals together is more than twice that of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.24.7210-7221.1995

    authors: Loroch AI,Nguyen BG,Ludwig RA

    更新日期:1995-12-01 00:00:00

  • Identification of staphylococcal hemolysins by an electrophoretic localization technique.

    abstract::A technique for identifying and characterizing staphylococcal hemolysins by first separating them electrophoretically in barbital-buffered agar gel (pH 8.4) at 5 ma/cm for 2 hr and then determining their hemolytic activities by exposing them to human, horse, rabbit, and sheep erythrocytes is described. The alpha-hemol...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.2.525-530.1967

    authors: Haque RU

    更新日期:1967-02-01 00:00:00

  • Stimulation of adenosine 5'-triphosphate-dependent in vitro deoxyribonucleic acid replication by factors from the periplasmic space of Escherichia coli.

    abstract::In vitro deoxyribonucleic acid (DNA) synthesis systems based on an earlier system using pencillin have been developed which use osmotic lysis of lysozyme-formed spheroplasts of Escherichia coli cells embedded in an agarose matrix. An adenosine 5'-triphosphate (ATP)-dependent semiconservative mode, or replicative mode,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.122.1.159-170.1975

    authors: Smith DW,Boerner P

    更新日期:1975-04-01 00:00:00

  • Identification of an Escherichia coli operon required for formation of the O-antigen capsule.

    abstract::Escherichia coli produces polysaccharide capsules that, based on their mechanisms of synthesis and assembly, have been classified into four groups. The group 4 capsule (G4C) polysaccharide is frequently identical to that of the cognate lipopolysaccharide O side chain and has, therefore, also been termed the O-antigen ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.15.5259-5266.2005

    authors: Peleg A,Shifrin Y,Ilan O,Nadler-Yona C,Nov S,Koby S,Baruch K,Altuvia S,Elgrably-Weiss M,Abe CM,Knutton S,Saper MA,Rosenshine I

    更新日期:2005-08-01 00:00:00

  • Measurement of adhesive forces between individual Staphylococcus aureus MSCRAMMs and protein-coated surfaces by use of optical tweezers.

    abstract::The force required to rupture bonds between individual Staphylococcus aureus MSCRAMMs and surfaces coated with extracellular matrix molecules has been quantified by using optical tweezers. The observed binding forces between fibrinogen or fibronectin and S. aureus MSCRAMMs occurred as an approximate integer multiple o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.6.2031-2035.2003

    authors: Simpson KH,Bowden G,Höök M,Anvari B

    更新日期:2003-03-01 00:00:00

  • Substrate specificity of the citrate transporter CitP of Lactococcus lactis.

    abstract::The citrate transporter CitP of lactic acid bacteria catalyzes electrogenic precursor-product exchange of citrate versus L-lactate during citrate-glucose cometabolism. In the absence of sugar, L-lactate is replaced by the metabolic intermediates/end products pyruvate, α-acetolactate, and acetate. In this study, the bi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00196-12

    authors: Pudlik AM,Lolkema JS

    更新日期:2012-07-01 00:00:00

  • Purification and characterization of the N-terminal domain of ExeA: a novel ATPase involved in the type II secretion pathway of Aeromonas hydrophila.

    abstract::Aeromonas hydrophila secretes a number of degradative enzymes and toxins into the external milieu via the type II secretory pathway or secreton. ExeA is an essential component of this system and is necessary for the localization and/or multimerization of the secretin ExeD. ExeA contains two sequence motifs characteris...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.18.6370-6378.2005

    authors: Schoenhofen IC,Li G,Strozen TG,Howard SP

    更新日期:2005-09-01 00:00:00

  • Genetic screen yields mutations in genes encoding all known components of the Escherichia coli signal recognition particle pathway.

    abstract::We describe the further utilization of a genetic screen that identifies mutations defective in the assembly of proteins into the Escherichia coli cytoplasmic membrane. The screen yielded mutations in each of the known genes encoding components of the E. coli signal recognition particle pathway: ffh, ffs, and ftsY, whi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.1.111-118.2002

    authors: Tian H,Beckwith J

    更新日期:2002-01-01 00:00:00

  • Thermal denaturation of mesophilic and thermophilic 5S ribonucleic acids.

    abstract::The Tm of Bacillus stearothermophilus 5S ribonucleic acid (RNA) is 1.5 +/- 0.5 C higher than that of 5S RNAs from B. subtilis and Escherichia coli. Melting in 50% methanol and in formaldehyde indicate that both base stacking and helical regions are involved in the slightly increased thermal stability of B. stearotherm...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.125.3.850-854.1976

    authors: Varricchio F,Marotta CA

    更新日期:1976-03-01 00:00:00

  • Functional characterization of SbmA, a bacterial inner membrane transporter required for importing the antimicrobial peptide Bac7(1-35).

    abstract::SbmA is an inner membrane protein of Gram-negative bacteria that is involved in the internalization of glycopeptides and prokaryotic and eukaryotic antimicrobial peptides, as well as of peptide nucleic acid (PNA) oligomers. The SbmA homolog BacA is required for the development of Sinorhizobium meliloti bacteroids with...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00818-13

    authors: Runti G,Lopez Ruiz Mdel C,Stoilova T,Hussain R,Jennions M,Choudhury HG,Benincasa M,Gennaro R,Beis K,Scocchi M

    更新日期:2013-12-01 00:00:00

  • Regulation of Bacillus subtilis sigmaH (spo0H) and AbrB in response to changes in external pH.

    abstract::The RNA polymerase sigma subunit, sigmaH, of Bacillus subtilis is required for the transcription of genes that are induced in late-growth cultures at high cell density, including genes that function in sporulation. The expression of sigmaH-controlled genes is repressed when nutrient broth sporulation medium (Difco spo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.21.6778-6787.1997

    authors: Cosby WM,Zuber P

    更新日期:1997-11-01 00:00:00

  • Localization of acyl carrier protein in Escherichia coli.

    abstract::Acyl carrier protein was localized by immunoelectron microscopy in the cytoplasm of Escherichia coli. These data are inconsistent with the previous report of an association between acyl carrier protein and the inner membrane (H. Van den Bosch, J.R. Williamson, and P.R. Vagelos, Nature [London] 228:338-341, 1970). More...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.1.5-8.1985

    authors: Jackowski S,Edwards HH,Davis D,Rock CO

    更新日期:1985-04-01 00:00:00

  • Cell division of cycle of Bacillus subtilis: evidence of variability in period D.

    abstract::In Bacillus subtilis the deoxyribonucleic acid content and the extent of cell division during inhibition of chromosome replication increased as a function of the average cell mass, independent of the growth rate. At each growth rate, mass, deoxyribonucleic acid, and residual division varied in different cultures. The ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.142.1.254-261.1980

    authors: Holmes M,Rickert M,Pierucci O

    更新日期:1980-04-01 00:00:00

  • Genome sequence of a serotype b non-JP2 aggregatibacter actinomycetemcomitans strain, ANH9381, from a periodontally healthy individual.

    abstract::Gram-negative Aggregatibacter actinomycetemcomitans can be distinguished (based on the promoter structure of the leukotoxin operon) into JP2 and non-JP2 genotypes, with the former found to be more pathogenic than the latter. Here we report the first complete genome sequence of a serotype b non-JP2 strain of A. actinom...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06770-11

    authors: Chen C,Kittichotirat W,Chen W,Downey JS,Bumgarner R

    更新日期:2012-04-01 00:00:00

  • The tolC locus of Escherichia coli affects the expression of three major outer membrane proteins.

    abstract::tolC mutants, which are resistant to colicin E1 and also highly sensitive to detergents and dyes, were shown to lack the OmpF outer membrane protein. There was little effect on transcription as judged by the use of an ompF-lac operon fusion strain, and the tolC effect was probably due to a post-transcriptional effect....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.150.3.1016-1023.1982

    authors: Morona R,Reeves P

    更新日期:1982-06-01 00:00:00