Abstract:
:No plasmid was detected in Xanthomonas campestris pv. campestris 17, a strain of the causative agent of black rot in cruciferous plants isolated in Taiwan. Its chromosome was cut by PacI, PmeI, and SwaI into five, two, and six fragments, respectively, and a size of 4.8 Mb was estimated by summing the fragment lengths in these digests. Based on the data obtained from partial digestion and Southern hybridization using probes common to pairs of the overlapping fragments or prepared from linking fragments, a circular physical map bearing the PacI, PmeI, and SwaI sites was constructed for the X. campestris pv. campestris 17 chromosome. Locations of eight eps loci involved in exopolysaccharide (xanthan gum) synthesis, two rrn operons each possessing an unique I-CeuI site, one pig cluster required for yellow pigmentation, and nine auxotrophic markers were determined, using mutants isolated by mutagenesis with Tn5(pfm)CmKm. This transposon contains a polylinker with sites for several rare-cutting restriction endonucleases located between the chloramphenicol resistance and kanamycin resistance (Kmr) genes, which upon insertion introduced additional sites into the chromosome. The recA and tdh genes, with known sequences, were mapped by tagging with the polylinker-Kmr segment from Tn5(pfm)CmKm. This is the first map for X. campestris and would be useful for genetic studies of this and related Xanthomonas species.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Tseng YH,Choy KT,Hung CH,Lin NT,Liu JY,Lou CH,Yang BY,Wen FS,Weng SF,Wu JRdoi
10.1128/JB.181.1.117-125.1999subject
Has Abstractpub_date
1999-01-01 00:00:00pages
117-25issue
1eissn
0021-9193issn
1098-5530journal_volume
181pub_type
杂志文章abstract::Bacteriophage lambda adsorbs to its Escherichia coli K-12 host by interacting with LamB, its cell-surface receptor. We fused C-terminal portions of J, the tail fiber protein of lambda, to maltose-binding protein. Solid-phase binding assays demonstrated that a purified fusion protein comprising only the last 249 residu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.2.508-512.2000
更新日期:2000-01-01 00:00:00
abstract::Cells of Pseudomonas putida NP, Pseudomonas species (NCIB 9816), and a Nocardia species, after growth on naphthalene as sole source of carbon and energy, contain a nicotinamide adenine dinucleotide (NAD+)-dependent enzyme that oxidizes cis-dihydrodiols of mono- and polycyclic aromatic compounds. Similarly, cells of a ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.128.3.842-850.1976
更新日期:1976-12-01 00:00:00
abstract::Sortases are transamidases that covalently link proteins to the peptidoglycan of gram-positive bacteria. The genome of the pathogenic bacterium Listeria monocytogenes encodes two sortases genes, srtA and srtB. The srtA gene product anchors internalin and some other LPXTG-containing proteins to the listerial surface. H...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.7.1972-1982.2004
更新日期:2004-04-01 00:00:00
abstract::The nucleotide sequence of the Escherichia coli colicin I receptor gene (cir) has been determined. The predicted mature protein consists of 599 amino acids and has a molecular weight of 67,169. Several previously noted characteristics of other E. coli outer membrane protein sequences were also identified in the sequen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.2.1041-1047.1989
更新日期:1989-02-01 00:00:00
abstract::Rhodobacter sphaeroides cells containing an in-frame deletion within ccmA lack detectable soluble and membrane-bound c-type cytochromes and are unable to grow under conditions where these proteins are required. Only strains merodiploid for ccmABCDG were found after attempting to generate cells containing either a ccmG...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.15.4643-4647.2001
更新日期:2001-08-01 00:00:00
abstract::Thiocyanate hydrolase is a newly found enzyme from Thiobacillus thioparus THI 115 that converts thiocyanate to carbonyl sulfide and ammonia (Y. Katayama, Y. Narahara, Y. Inoue, F. Amano, T. Kanagawa, and H. Kuraishi, J. Biol. Chem. 267:9170-9175, 1992). We have cloned and sequenced the scn genes that encode the three ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.10.2583-2589.1998
更新日期:1998-05-01 00:00:00
abstract::Lundgren, D. G. (Syracuse University, Syracuse, N.Y.) and J. J. Cooney. Chemical analyses of asporogenic mutants of Bacillus cereus. J. Bacteriol. 83:1287-1293. 1962.-Bacillus cereus ATCC 4342 and three temperature-sensitive asporogenic mutants were compared regarding some basic cellular components and response to met...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.6.1287-1293.1962
更新日期:1962-06-01 00:00:00
abstract::Outer membrane alterations were characterized in spontaneous mutants of the Erwinia chrysanthemi 3937jRH, which were selected for resistance to bacteriophage phi EC2. All but one of the mutants analyzed were affected in their lipopolysaccharide (LPS) structure, lacking the entire heterogeneous region of apparent high ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.9.4011-4017.1987
更新日期:1987-09-01 00:00:00
abstract::Naegleria fowleri is a pathogenic amoeboflagellate that can be evoked to transform from amoebae to flagellates by subculture to nonnutrient buffer. More than half of the amoebae of strains KUL, nN68, and Lovell became enflagellated 300 min after subculture to amoeba-saline, whereas no amoebae of strains NF66, NF69, an...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1982-11-01 00:00:00
abstract::The effect of the iron content of the medium on the yields of extracellular products by seven distinct strains of Pseudomonas aeruginosa was examined. All strains showed at least an 85% decrease in toxin A yields when grown in medium containing 5.0 mug of iron per ml (high iron) as compared to 0.05 mug/ml (low iron), ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.1.193-200.1979
更新日期:1979-04-01 00:00:00
abstract::Specific growth rates as low as 0.005 hr(-1) (generation times of 20 to 200 hr) of aquatic bacteria in natural waters have been calculated from significant differences between dilution rates and washout rates in a chemostat. The measured growth rates were affected by the treatment of the water samples (type of sterili...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.99.1.156-160.1969
更新日期:1969-07-01 00:00:00
abstract::Cysteine and methionine availability influences many processes in the cell. In bacteria, transcription of the specific genes involved in the synthesis of these two amino acids is usually regulated by different mechanisms or regulators. Pathways for the synthesis of cysteine and methionine and their interconversion wer...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.11.3762-3778.2005
更新日期:2005-06-01 00:00:00
abstract::In vitro transcription from the promoter for the nitrogen fixation regulatory operon nifLA of K. pneumoniae requires four protein fractions: the core form of RNA polymerase; NTRA, an alternate sigma factor; NTRC, an auxiliary DNA-binding protein; and NTRB, a bifunctional enzyme that controls the activity of NTRC by co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.6.2876-2880.1987
更新日期:1987-06-01 00:00:00
abstract:UNLABELLED:Bacterial type IV coupling proteins (T4CPs) bind and mediate the delivery of DNA substrates through associated type IV secretion systems (T4SSs). T4CPs consist of a transmembrane domain, a conserved nucleotide-binding domain (NBD), and a sequence-variable helical bundle called the all-alpha domain (AAD). In ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00189-15
更新日期:2015-07-01 00:00:00
abstract::Proline-requiring mutants of Saccharomyces cerevisiae were isolated. Each mutation is recessive and is inherited as expected for a single nuclear gene. Three complementation groups cold be defined which are believed to correspond to mutations in the three genes (pro1, pro2, and pro3) coding for the three enzymes of th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.3.816-822.1979
更新日期:1979-06-01 00:00:00
abstract::The Azorhizobium nifA promoter (PnifA) is positively regulated by two physiological signal transduction pathways, NtrBC, which signals anabolic N status, and FixLJK, which signals prevailing O2 status. Yet, PnifA response (gene product per unit time) to these two activating signals together is more than twice that of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.24.7210-7221.1995
更新日期:1995-12-01 00:00:00
abstract::A technique for identifying and characterizing staphylococcal hemolysins by first separating them electrophoretically in barbital-buffered agar gel (pH 8.4) at 5 ma/cm for 2 hr and then determining their hemolytic activities by exposing them to human, horse, rabbit, and sheep erythrocytes is described. The alpha-hemol...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.2.525-530.1967
更新日期:1967-02-01 00:00:00
abstract::In vitro deoxyribonucleic acid (DNA) synthesis systems based on an earlier system using pencillin have been developed which use osmotic lysis of lysozyme-formed spheroplasts of Escherichia coli cells embedded in an agarose matrix. An adenosine 5'-triphosphate (ATP)-dependent semiconservative mode, or replicative mode,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.122.1.159-170.1975
更新日期:1975-04-01 00:00:00
abstract::Escherichia coli produces polysaccharide capsules that, based on their mechanisms of synthesis and assembly, have been classified into four groups. The group 4 capsule (G4C) polysaccharide is frequently identical to that of the cognate lipopolysaccharide O side chain and has, therefore, also been termed the O-antigen ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.15.5259-5266.2005
更新日期:2005-08-01 00:00:00
abstract::The force required to rupture bonds between individual Staphylococcus aureus MSCRAMMs and surfaces coated with extracellular matrix molecules has been quantified by using optical tweezers. The observed binding forces between fibrinogen or fibronectin and S. aureus MSCRAMMs occurred as an approximate integer multiple o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.6.2031-2035.2003
更新日期:2003-03-01 00:00:00
abstract::The citrate transporter CitP of lactic acid bacteria catalyzes electrogenic precursor-product exchange of citrate versus L-lactate during citrate-glucose cometabolism. In the absence of sugar, L-lactate is replaced by the metabolic intermediates/end products pyruvate, α-acetolactate, and acetate. In this study, the bi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00196-12
更新日期:2012-07-01 00:00:00
abstract::Aeromonas hydrophila secretes a number of degradative enzymes and toxins into the external milieu via the type II secretory pathway or secreton. ExeA is an essential component of this system and is necessary for the localization and/or multimerization of the secretin ExeD. ExeA contains two sequence motifs characteris...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.18.6370-6378.2005
更新日期:2005-09-01 00:00:00
abstract::We describe the further utilization of a genetic screen that identifies mutations defective in the assembly of proteins into the Escherichia coli cytoplasmic membrane. The screen yielded mutations in each of the known genes encoding components of the E. coli signal recognition particle pathway: ffh, ffs, and ftsY, whi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.1.111-118.2002
更新日期:2002-01-01 00:00:00
abstract::The Tm of Bacillus stearothermophilus 5S ribonucleic acid (RNA) is 1.5 +/- 0.5 C higher than that of 5S RNAs from B. subtilis and Escherichia coli. Melting in 50% methanol and in formaldehyde indicate that both base stacking and helical regions are involved in the slightly increased thermal stability of B. stearotherm...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.3.850-854.1976
更新日期:1976-03-01 00:00:00
abstract::SbmA is an inner membrane protein of Gram-negative bacteria that is involved in the internalization of glycopeptides and prokaryotic and eukaryotic antimicrobial peptides, as well as of peptide nucleic acid (PNA) oligomers. The SbmA homolog BacA is required for the development of Sinorhizobium meliloti bacteroids with...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00818-13
更新日期:2013-12-01 00:00:00
abstract::The RNA polymerase sigma subunit, sigmaH, of Bacillus subtilis is required for the transcription of genes that are induced in late-growth cultures at high cell density, including genes that function in sporulation. The expression of sigmaH-controlled genes is repressed when nutrient broth sporulation medium (Difco spo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.21.6778-6787.1997
更新日期:1997-11-01 00:00:00
abstract::Acyl carrier protein was localized by immunoelectron microscopy in the cytoplasm of Escherichia coli. These data are inconsistent with the previous report of an association between acyl carrier protein and the inner membrane (H. Van den Bosch, J.R. Williamson, and P.R. Vagelos, Nature [London] 228:338-341, 1970). More...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.1.5-8.1985
更新日期:1985-04-01 00:00:00
abstract::In Bacillus subtilis the deoxyribonucleic acid content and the extent of cell division during inhibition of chromosome replication increased as a function of the average cell mass, independent of the growth rate. At each growth rate, mass, deoxyribonucleic acid, and residual division varied in different cultures. The ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.142.1.254-261.1980
更新日期:1980-04-01 00:00:00
abstract::Gram-negative Aggregatibacter actinomycetemcomitans can be distinguished (based on the promoter structure of the leukotoxin operon) into JP2 and non-JP2 genotypes, with the former found to be more pathogenic than the latter. Here we report the first complete genome sequence of a serotype b non-JP2 strain of A. actinom...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06770-11
更新日期:2012-04-01 00:00:00
abstract::tolC mutants, which are resistant to colicin E1 and also highly sensitive to detergents and dyes, were shown to lack the OmpF outer membrane protein. There was little effect on transcription as judged by the use of an ompF-lac operon fusion strain, and the tolC effect was probably due to a post-transcriptional effect....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.150.3.1016-1023.1982
更新日期:1982-06-01 00:00:00