Abstract:
:A strain of Pseudomonas (P. putida HS1) was found to resemble P. putida (arvilla) mt-2 in its ability to degrade toluene, m- and p-xylene, 1,2,4-trimethylbenzene (pseudocumene), and 3-ethyltoluene via oxidation of a methyl substituent and reactions of the meta-fission pathway. The ability to degrade these substrates by P. putida HS1 (PpC1) was shown to be encoded by a TOL (pDK1) plasmid as evidenced by: (i) spontaneous loss of the TOL-related phenotype after growth with benzoate, (ii) transfer of the TOL character from the wild type into cured recipients by conjugation, and (iii) isolation of a plasmid of identical molecular weight (120 X 10(6)) from both the wild type and an exconjugant obtained by mating wild type with a putative cured recipient. In addition to the isolation of apparent cured strains having lost the entire TOL-related phenotype, two additional mutant classes were observed after growth on benzoate. One class, represented by PpCT1, was unable to utilize the alkyl-substituted aromatic compounds but retained the ability to grow with toluene and benzyl alcohol. Analysis of PpCT1 revealed that it was unable to synthesize the TOL-encoded toluate oxidase and enzymes of the meta pathway but retained the ability to elaborate activities for toluene hydroxylase, benzyl alcohol, and benzaldehyde dehydrogenase, thereby mediating initial oxidation of toluene to benzoate, which was then further metabolized via enzymes of the chromosomally encoded ortho-fission pathway. A second class of mutants had lost the ability to utilize the hydrocarbons but could still grow with m-toluate but not p-toluate, 3,4-dimethylbenzoate, or 3-ethylbenzoate, intermediates in the oxidation of the corresponding hydrocarbons. Our such mutant, PpCM1, could no longer synthesize enzymes required for initial oxidation of the hydrocarbons, but was able to produce the toluate oxidase and enzymes of the meta pathway, thereby facilitating degradation of m-toluate. Neither PpCT1, PpCM1, nor a putative cured strain, PpCC1, reverted at detectable frequencies (less than 10(-9). Analysis of each strain for plasmid deoxyribonucleic acid revealed the presence of a single plasmid in each strain with the following molecular weights: PpCM1, 100 X 10(6) (pDKM1); PpCT1, 80 X 10(6) (pDKT1); PpCC1 20 X 10(6) (pDKC1). The results suggest that the TOL (pDK1) plasmid has undergone deletions giving rise to smaller replicons which either encode for only a fraction of the wild-type catabolic functions (pDKM1, pDKT1) or have lost all catabolic activities (pDKC1).
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Kunz DA,Chapman PJdoi
10.1128/JB.146.3.952-964.1981subject
Has Abstractpub_date
1981-06-01 00:00:00pages
952-64issue
3eissn
0021-9193issn
1098-5530journal_volume
146pub_type
杂志文章abstract::Streptococcus bovis JB1 cells energized with glucose transported glutamine at a rate of 7 nmol/mg of protein per min at a pH of 5.0 to 7.5; sodium had little effect on the transport rate. Because valinomycin-treated cells loaded with K and diluted into Na (pH 6.5) to create an artificial delta psi took up little gluta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.6.2981-2985.1989
更新日期:1989-06-01 00:00:00
abstract::The application of a simple negative staining technique and freeze-etching has revealed detailed information on the species-specific surface structure of spores and aerial hyphae of streptomycetes. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.101.1.318-322.1970
更新日期:1970-01-01 00:00:00
abstract::Nitrogen fixation occurs in two domains, Archaea and Bacteria. We have characterized a nif (nitrogen fixation) gene cluster in the methanogenic archaeon Methanococcus maripaludis. Sequence analysis revealed eight genes, six with sequence similarity to known nif genes and two with sequence similarity to glnB. The gene ...
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pub_type: 杂志文章
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更新日期:1998-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.6.1867-1871.1997
更新日期:1997-03-01 00:00:00
abstract::Methods were devised to purify the cell-associated, iron-binding compounds known as mycobactins from the closely related species Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum (i.e., the MAIS complex of organisms). The mycobactins from these three species showed a structure that is c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.2.896-903.1985
更新日期:1985-11-01 00:00:00
abstract::The structural properties required for the binding of peptide substrates to the Escherichia coli periplasmic protein involved in oligopeptide transport were surveyed by measuring the ability of different peptides to compete for binding in an equilibrium dialysis assay with the tripeptide Ala-Phe-[3H]Gly. The protein s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.2.775-779.1986
更新日期:1986-11-01 00:00:00
abstract::The glycan chains in peptidoglycan or murein are cross-linked by transpeptidation of the peptide side chains. To assess the fraction of side chains involved in cross-bridges, distinction has been made between cross-linkage and cross-linking. The first expression refers to the situation in unlabeled (or fully labeled) ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.6.2028-2031.1992
更新日期:1992-03-01 00:00:00
abstract::Transcriptional factor CarD is the only reported prokaryotic analog of eukaryotic high-mobility-group A (HMGA) proteins, in that it has contiguous acidic and AT hook DNA-binding segments and multifunctional roles in Myxococcus xanthus carotenogenesis and fruiting body formation. HMGA proteins are small, randomly struc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.12.3527-3537.2003
更新日期:2003-06-01 00:00:00
abstract::Virulent bacteria of the genus Yersinia secrete a number of virulence determinants called Yops. These proteins lack typical signal sequences and are not posttranslationally processed. Two gene loci have been identified as being involved in the specific Yop secretion system (G. Cornelis, p. 231-265, In C. E. Hormache, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.9.2619-2626.1994
更新日期:1994-05-01 00:00:00
abstract::The syrB gene is required for syringomycin production by Pseudomonas syringae pv. syringae and full virulence during plant pathogenesis. Strain B3AR132 containing a syrB::lacZ fusion was used to detect transcriptional activation of the syrB gene in syringomycin minimal medium by plant metabolites with signal activity....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.18.5784-5792.1991
更新日期:1991-09-01 00:00:00
abstract::[3H]Diaminopimelic acid (Dap) was incorporated exclusively into peptidoglycan by Escherichia coli strains auxotrophic for both lysine and Dap. The rate of [3H]Dap incorporation by stringent (rel+) strains was significantly decreased when cells were deprived of required amino acids. The addition of chloramphenicol to a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.127.3.1119-1126.1976
更新日期:1976-09-01 00:00:00
abstract::From the cell envelope preparation of Sphingomonas paucimobilis two membrane fractions with different densities were separated by sucrose density gradient ultracentrifugation. The high-density fraction contained several major proteins, phospholipids, and glycosphingolipids, which are the only glycolipids of this lipop...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.2.284-290.1994
更新日期:1994-01-01 00:00:00
abstract::Soluble c-type cytochromes are central to metabolism of C1 compounds in methylotrophic bacteria. In order to characterize the role of c-type cytochromes in methane-utilizing bacteria (methanotrophs), we have purified four different cytochromes, cytochromes c-554, c-553, c-552, and c-551, from the marine methanotroph M...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.9.5360-5367.1990
更新日期:1990-09-01 00:00:00
abstract::Pentalenolactone (PL) irreversibly inactivates the enzyme glyceraldehyde-3-phosphate dehydrogenase [D-glyceraldehyde-3-phosphate:NAD+ oxidoreductase (phosphorylating)] (EC 1.2.1.12) and thus is a potent inhibitor of glycolysis in both procaryotic and eucaryotic cells. We showed that PL-producing strain Streptomyces ar...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.2.930-936.1983
更新日期:1983-02-01 00:00:00
abstract::Tn4551, a clindamycin resistance (Ccr) transposon from the R plasmid pBI136, was cloned onto an Escherichia coli-Bacteroides shuttle vector which could replicate normally in E. coli but was maintained unstably in Bacteroides fragilis. To aid in cloning and to ensure maintenance of Tn4551 in E. coli, a kanamycin resist...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.8.3450-3457.1987
更新日期:1987-08-01 00:00:00
abstract::The opportunistic pathogen Pseudomonas aeruginosa utilizes a type III secretion system (T3SS) to evade phagocytosis and damage eukaryotic cells. Transcription of the T3SS regulon is controlled by ExsA, a member of the AraC/XylS family of transcriptional regulators. These family members generally consist of an approxim...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00002-09
更新日期:2009-06-01 00:00:00
abstract::Yeast mutants deficient in orotidine-5'-phosphate (OMP) pyrophosphorylase activity have been obtained. The gene encoding this enzyme is unlinked to the four pyrimidine genes previously described. The specific activity of OMP pyrophosphorylase remains constant under all of the physiological conditions used to repress, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.109.1.196-202.1972
更新日期:1972-01-01 00:00:00
abstract::Neisseria meningitidis serogroup B strain M986 was examined for the involvement of lipid intermediate(s) participating in the biosynthesis of the sialic acid capsular polysaccharide. The addition of exogenous undecaprenyl phosphate, phosphatidylethanolamine, or phosphatidylglycerol to particulate membranes, in the pre...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.161.3.861-867.1985
更新日期:1985-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.2.630-644.2003
更新日期:2003-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.115.1.205-212.1973
更新日期:1973-07-01 00:00:00
abstract::A population of Bacillus subtilis can be fractionated into at least two components on the basis of their buoyant densities on a Renografin gradient. In competent cultures, most, and perhaps all, of the competent bacteria appear in the lighter fraction which composes 2 to 10% of the bulk population. These "lighter" bac...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.3.867-875.1968
更新日期:1968-03-01 00:00:00
abstract::In Escherichia coli, ClpYQ (HslUV) is a two-component ATP-dependent protease composed of ClpY (HslU), an ATPase with unfolding activity, and ClpQ (HslV), a peptidase. In the ClpYQ proteolytic complex, the hexameric rings of ClpY (HslU) are responsible for protein recognition, unfolding, and translocation into the prot...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05128-11
更新日期:2011-10-01 00:00:00
abstract::Campylobacter jejuni is a leading food-borne pathogen causing gastroenteritis in humans. Although OxyR is a widespread oxidative stress regulator in many Gram-negative bacteria, C. jejuni lacks OxyR and instead possesses the metalloregulator PerR. Despite the important role played by PerR in oxidative stress defense, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05493-11
更新日期:2011-11-01 00:00:00
abstract::Mutations in carbohydrate-negative mutants of Pseudomonas aeruginosa PAO1 individually deficient in glucose 6-phosphate dehydrogenase (zwf), 6-phosphogluconate dehydratase (edd), or pyruvate carboxylase (pyc) were mapped on the chromosome by plasmid R68.45-mediated conjugation and by bacteriophage F116L-mediated trans...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.3.1123-1129.1983
更新日期:1983-12-01 00:00:00
abstract::The segregation of mating loci with other unselected genes was analyzed in recombinants obtained from matings of Nocardia canicruria and N. erythropolis. The loci C/c and E/e control nocardial compatibility. Four mating genotype combinations were observed: cE, Ce, CE, and ce. Strains of N. erythropolis bear the genoty...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.99.1.25-36.1969
更新日期:1969-07-01 00:00:00
abstract::The enzyme pattern of Saccharomyces cerevisiae was followed during batch growth and in continuous culture in a synthetic medium limited for glucose under aerobic conditions. Seven enzymes were measured: succinate-cytochrome c oxidoreductase, malate dehydrogenase, nicotinamide adenine dinucleotide-linked glutamate dehy...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.2.479-486.1968
更新日期:1968-08-01 00:00:00
abstract::Eight mutants of Bacillus subtilis deficient in xylan utilization were isolated and characterized genetically and biochemically. Each mutant was obtained independently after nitrosoguanidine mutagenesis. All of the analyzed mutations were shown to be linked. Reciprocal transformation crosses revealed the existence of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.1.257-263.1983
更新日期:1983-10-01 00:00:00
abstract::Buck, C. A. (Kansas State University, Manhattan), R. L. Anacker, F. S. Newman, and A. Eisenstark. Phage isolated from lysogenic Bacillus anthracis. J. Bacteriol. 85:1423-1430. 1963.-Bacillus anthracis cultures (a total of 311) were obtained from several laboratories throughout the world; 139 of these were screened for...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.6.1423-1430.1963
更新日期:1963-06-01 00:00:00
abstract::Two acid-inducible genes, aniC and aciK, that require anaerobiosis and tyrosine for expression were identified as orf326a encoding a potential amino acid/polyamine antiporter and hyaB encoding hydrogenase I, respectively. Cyclic AMP (cAMP) receptor protein, cAMP, and TyrR, regulator of aromatic amino acid metabolism, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.2.689-694.1999
更新日期:1999-01-01 00:00:00
abstract::Here we present the genome sequence of Rhizobium grahamii CCGE502. R. grahamii groups with other newly described broad-host-range species, which are not very efficient Phaseolus vulgaris symbionts, with a wide geographic distribution and which constitutes a novel Rhizobium clade. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01785-12
更新日期:2012-12-01 00:00:00