Fractionation of transformable bacteria from ocompetent cultures of Bacillus subtilis on renografin gradients.

Abstract:

:A population of Bacillus subtilis can be fractionated into at least two components on the basis of their buoyant densities on a Renografin gradient. In competent cultures, most, and perhaps all, of the competent bacteria appear in the lighter fraction which composes 2 to 10% of the bulk population. These "lighter" bacteria are the only bacteria capable of incorporating transforming deoxyribonucleic acid.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Cahn FH,Fox MS

doi

10.1128/JB.95.3.867-875.1968

subject

Has Abstract

pub_date

1968-03-01 00:00:00

pages

867-75

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

95

pub_type

杂志文章
  • Metabolism of biphenyl by Aspergillus toxicarius: induction of hydroxylating activity and accumulation of water-soluble conjugates.

    abstract::Biphenyl metabolism in Aspergillus toxicarius occurs by successive hydroxylations in the 4- and 4'-positions, followed by conjugation with sulfate to produce 4-hydroxybiphenyl-O-sulfonic acid and 4,4'-dihydroxybiphenyl-O-sulfonic acid. The hydroxylation reactions normally occur only after a prolonged lag period after ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.156.1.49-57.1983

    authors: Golbeck JH,Albaugh SA,Radmer R

    更新日期:1983-10-01 00:00:00

  • Complete genome sequence of the type strain Cupriavidus necator N-1.

    abstract::Here we announce the complete genome sequence of the copper-resistant bacterium Cupriavidus necator N-1, the type strain of the genus Cupriavidus. The genome consists of two chromosomes and two circular plasmids. Based on genome comparison, the chromosomes of C. necator N-1 share a high degree of similarity with the t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05660-11

    authors: Poehlein A,Kusian B,Friedrich B,Daniel R,Bowien B

    更新日期:2011-09-01 00:00:00

  • Subunits of the alkaline phosphatase of Bacillus licheniformis: chemical, physicochemical, and dissociation studies.

    abstract::The alkaline phosphatase (orthophosphoric monoester phosphydrolase, EC 3.1.3.1) of Bacillus licheniformis MC14 was studied in an attempt to determine the number of subunits contained in the 120,000-molecular-weight native enzyme. Two moles of arginine was liberated per mole of native enzyme by carboxypeptidases A and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.128.2.651-657.1976

    authors: Hulett FM,Schaffel SD,Campbell LL

    更新日期:1976-11-01 00:00:00

  • Evolutionary relationships among pathogenic Candida species and relatives.

    abstract::Small subunit rRNA sequences have been determined for 10 of the most clinically important pathogenic species of the yeast genus Candida (including Torulopsis [Candida] glabrata and Yarrowia [Candida] lipolytica) and for Hansenula polymorpha. Phylogenetic analyses of these sequences and those of Saccharomyces cerevisia...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.7.2250-2255.1991

    authors: Barns SM,Lane DJ,Sogin ML,Bibeau C,Weisburg WG

    更新日期:1991-04-01 00:00:00

  • Tyrosine phosphorylation of a membrane protein from Pseudomonas solanacearum.

    abstract::We have investigated a tyrosine kinase activity from Pseudomonas solanacearum, an economically important plant pathogen. In vitro incubation of membrane fractions with [gamma-32P]ATP and subsequent sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed an 85-kDa phosphoprotein. Phosphorylation of this prot...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.13.4356-4360.1992

    authors: Atkinson M,Allen C,Sequeira L

    更新日期:1992-07-01 00:00:00

  • Ultraviolet-sensitive mutator strain of Escherichia coli K-12.

    abstract::An ultraviolet (UV)-sensitive mutator gene, mutU, was identified in Escherichia coli K-12. The mutation mutU4 is very close to uvrD, between metE and ilv, on the E. coli chromosome. It was recessive as a mutator and as a UV-sensitive mutation. The frequency of reversion of trpA46 on an F episome was increased by mutU4...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.1.145-160.1973

    authors: Siegel EC

    更新日期:1973-01-01 00:00:00

  • Regulation of galactose operon expression: glucose effects and role of cyclic adenosine 3',5'-monophosphate.

    abstract::We studied the following two aspects of the glucose effect on galactose operon expression in Escherichia coli K-12: catabolite repression and inducer exclusion. Using both inducible and constitutive strains and measuring the rate of promoter-proximal enzyme synthesis, we found that the galactose operon did not seem to...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.146.1.149-154.1981

    authors: Joseph E,Danchin A,Ullmann A

    更新日期:1981-04-01 00:00:00

  • Transductional analysis of Pseudomonas aeruginosa methionineless auxotrophs.

    abstract::Seventy-one methionineless and cysteineless auxotrophs of Pseudomonas aeruginosa were placed into nine groups on the basis of their growth on methionine precursors and the cross-feeding response. Transduction experiments with bacteriophage F116 indicated the presence of four linkage groups among the methionineless mut...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.97.1.210-216.1969

    authors: Calhoun DH,Feary TW

    更新日期:1969-01-01 00:00:00

  • Decay of a model mRNA in Bacillus subtilis by a combination of RNase J1 5' exonuclease and RNase Y endonuclease activities.

    abstract::The involvement of the recently characterized 5' exonuclease activity of RNase J1 and endonuclease activity of RNase Y in the turnover of ΔermC mRNA in Bacillus subtilis was investigated. Evidence is presented that both of these activities determine the half-life of ΔermC mRNA. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05939-11

    authors: Yao S,Richards J,Belasco JG,Bechhofer DH

    更新日期:2011-11-01 00:00:00

  • Natural transformation in Campylobacter species.

    abstract::Growing cells of Campylobacter coli and C. jejuni were naturally transformed by naked DNA without the requirement for any special treatment. Transformation frequencies for homologous chromosomal DNA were approximately 10(-3) transformants per recipient cell in C. coli and 10(-4) in C. jejuni. Maximum competence was fo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.2.949-955.1990

    authors: Wang Y,Taylor DE

    更新日期:1990-02-01 00:00:00

  • Imprecise excision of plasmid pE194 from the chromosomes of Bacillus subtilis pE194 insertion strains.

    abstract::Plasmid pE194 has been shown to be rescued by integration after cultivation of infected Bacillus subtilis recE4 cells at a restrictive high temperature. The plasmid is also spontaneously excised from the chromosome at a low frequency by precise or imprecise excision (J. Hofemeister, M. Israeli-Reches, and D. Dubnau, M...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.21.6997-7002.1992

    authors: Conrad B,Bashkirov VI,Hofemeister J

    更新日期:1992-11-01 00:00:00

  • Hybridization studies reveal homologies between pBF4 and pBFTM10, Two clindamycin-erythromycin resistance transfer plasmids of Bacteroides fragilis.

    abstract::Two clindamycin-erythromycin resistance transfer factors of Bacteroides fragilis, pBF4 and pBFTM10, were analyzed for regions of DNA homology. Although the plasmids were derived from different clinical isolates of B. fragilis and have different sizes, they showed homology in the clindamycin-erythromycin resistance reg...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Shimell MJ,Smith CJ,Tally FP,Macrina FL,Malamy MH

    更新日期:1982-11-01 00:00:00

  • Dienelactone hydrolase from Pseudomonas cepacia.

    abstract::Dienelactone hydrolases have previously been shown to play a crucial role in chlorocatechol degradation via the modified ortho cleavage pathway. Recently, the enzymes induced in 4-fluorobenzoate-utilizing bacteria have been classified into three groups on the basis of their specificity towards cis- and trans-dienelact...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.10.2994-3001.1993

    authors: Schlömann M,Ngai KL,Ornston LN,Knackmuss HJ

    更新日期:1993-05-01 00:00:00

  • Spheroplast fusion and heterokaryon formation in Mucor racemosus.

    abstract::Heterokaryons of Mucor racemosus were produced by fusion of spheroplasts from two auxotrophic strains of the fungus. Germinated sporangiospores were converted to spheroplasts by using commercial chitinase and Myxobacter AL-1 chitosanase. Spheroplasts from the auxotrophic strains were mixed in a buffered Ca(NO3)2 solut...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.134.1.349-352.1978

    authors: Genthner FJ,Borgia PT

    更新日期:1978-04-01 00:00:00

  • Bacterial plasmids that carry two functional centromere analogs are stable and are partitioned faithfully.

    abstract::The par genes of unit-copy plasmids P1 and F promote equitable distribution of plasmid copies to daughter cells and can be considered to be functional analogs of eucaryotic centromeres. Composite plasmids were constructed which carry either two functional P1 par regions or one F and one P1 region. Unlike dicentric chr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.158.2.742-745.1984

    authors: Austin SJ

    更新日期:1984-05-01 00:00:00

  • Presence of polyphosphate of low molecular weight in zygomycetes.

    abstract::Polyphosphate of average chain length corresponding to 10 phosphate units was detected in the mycelial extract of zygomycetes. Gel electrophoresis techniques commonly used for the separation and characterization of acidic mucopolysaccharides were successfully used for the detection, purification, and characterization ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.127.3.1408-1413.1976

    authors: Dietrich SM

    更新日期:1976-09-01 00:00:00

  • New outer membrane-associated protease of Escherichia coli K-12.

    abstract::The gene for a new outer membrane-associated protease, designated OmpP, of Escherichia coli has been cloned and sequenced. The gene encodes a 315-residue precursor protein possessing a 23-residue signal sequence. Including conservative substitutions and omitting the signal peptides, OmpP is 87% identical to the outer ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.2.359-367.1994

    authors: Kaufmann A,Stierhof YD,Henning U

    更新日期:1994-01-01 00:00:00

  • Cytology of spore formation in Clostridium perfringens.

    abstract::The sequential morphological events in spore formation by Clostridium perfringens type D were observed in Ellner's medium where 80 to 100% of the cells formed spores. Gross structural changes were studied with the light microscope under phase-contrast, and in fixed cells by the use of both nigrosin and Giemsa preparat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.5.1818-1834.1968

    authors: Hoeniger JF,Stuart PF,Holt SC

    更新日期:1968-11-01 00:00:00

  • Enzymatic characterization and in vivo function of five terminal oxidases in Pseudomonas aeruginosa.

    abstract::The ubiquitous opportunistic pathogen Pseudomonas aeruginosa has five aerobic terminal oxidases: bo(3)-type quinol oxidase (Cyo), cyanide-insensitive oxidase (CIO), aa3-type cytochrome c oxidase (aa3), and two cbb(3)-type cytochrome c oxidases (cbb(3)-1and cbb(3)-2). These terminal oxidases are differentially regulate...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02176-14

    authors: Arai H,Kawakami T,Osamura T,Hirai T,Sakai Y,Ishii M

    更新日期:2014-12-01 00:00:00

  • Chemical modification of Streptococcus flagellar motors.

    abstract::Video techniques were used to record changes in motility of cells of Streptococcus sp. strain V4051 exposed to a variety of protein modification reagents. Starved cells were tethered to glass by a single flagellum, energized metabolically with glucose, or treated with valinomycin and energized artificially via shifts ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.158.3.832-843.1984

    authors: Conley MP,Berg HC

    更新日期:1984-06-01 00:00:00

  • Serological comparison of ten glycolytic Mycoplasma species.

    abstract::Seventeen strains of mycoplasmata representing 11 named species were compared serologically by three parameters: growth inhibition on agar, double immunodiffusion, and complement fixation. In growth-inhibition studies, a strain labeled Mycoplasma histotropicum was found related to and perhaps best classified as M. pul...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.3.1044-1055.1969

    authors: Kenny GE

    更新日期:1969-06-01 00:00:00

  • Biosynthesis of phospholipids in Bacillus megaterium.

    abstract::Information on the biosynthesis of phospholipids in bacteria has been derived principally from the study of Escherichia coli and other gram-negative organisms. We have now carried out a detailed study of the pathways of phospholipid biosynthesis in the gram-positive organism Bacillus megarterium KM in relation to inve...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.3.996-1007.1979

    authors: Langley KE,Yaffe MP,Kennedy EP

    更新日期:1979-12-01 00:00:00

  • Constricted flux through the branched-chain amino acid biosynthetic enzyme acetolactate synthase triggers elevated expression of genes regulated by rpoS and internal acidification.

    abstract::The first common enzyme of isoleucine and valine biosynthesis, acetolactate synthase (ALS), is specifically inhibited by the herbicide sulfometuron methyl (SM). To further understand the physiological consequences of flux alterations at this point in metabolism, Escherichia coli genes whose expression was induced by p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.4.785-792.1998

    authors: Van Dyk TK,Ayers BL,Morgan RW,Larossa RA

    更新日期:1998-02-01 00:00:00

  • Structural characterization and assembly of the distal tail structure of the temperate lactococcal bacteriophage TP901-1.

    abstract::The tail structures of bacteriophages infecting gram-positive bacteria are largely unexplored, although the phage tail mediates the initial interaction with the host cell. The temperate Lactococcus lactis phage TP901-1 of the Siphoviridae family has a long noncontractile tail with a distal baseplate. In the present st...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.12.4187-4197.2005

    authors: Vegge CS,Brøndsted L,Neve H,Mc Grath S,van Sinderen D,Vogensen FK

    更新日期:2005-06-01 00:00:00

  • AmiC functions as an N-acetylmuramyl-l-alanine amidase necessary for cell separation and can promote autolysis in Neisseria gonorrhoeae.

    abstract::Neisseria gonorrhoeae is prone to undergo autolysis under many conditions not conducive to growth. The role of autolysis during gonococcal infection is not known, but possible advantages for the bacterial population include provision of nutrients to a starving population, modulation of the host immune response by rele...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00724-06

    authors: Garcia DL,Dillard JP

    更新日期:2006-10-01 00:00:00

  • Genome of the cyanobacterium Microcoleus vaginatus FGP-2, a photosynthetic ecosystem engineer of arid land soil biocrusts worldwide.

    abstract::The filamentous cyanobacterium Microcoleus vaginatusis found in arid land soils worldwide. The genome of M. vaginatus strain FGP-2 allows exploration of genes involved in photosynthesis, desiccation tolerance, alkane production, and other features contributing to this organism's ability to function as a major componen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05138-11

    authors: Starkenburg SR,Reitenga KG,Freitas T,Johnson S,Chain PS,Garcia-Pichel F,Kuske CR

    更新日期:2011-09-01 00:00:00

  • Bacteriophage clearance in the oyster (Crassotrea virginica).

    abstract::Since relatively few quantitative immunological studies had been conducted with invertebrates, this study was designed to learn more about the immune potential of the oyster, a molluscan representative. Experiments measured the primary and secondary clearance rates of T2 coliphage in the oyster. The fate of phage inje...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.4.1260-1266.1968

    authors: Acton RT,Evans EE

    更新日期:1968-04-01 00:00:00

  • Characterization of pilin genes from seven serologically defined prototype strains of Moraxella bovis.

    abstract::Numerous field isolates of Moraxella bovis have previously been classified by serological techniques into seven serogroups, each defined by homologous cross-reaction with antisera prepared against purified pili of a single prototype strain. The gene encoding pilin from each of the prototype strains has been characteri...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.16.4875-4882.1994

    authors: Atwell JL,Tennent JM,Lepper AW,Elleman TC

    更新日期:1994-08-01 00:00:00

  • Two genes for carbohydrate catabolism are divergently transcribed from a region of DNA containing the hexC locus in Pseudomonas aeruginosa PAO1.

    abstract::The hexC locus of Pseudomonas aeruginosa PAO1 was localized to a 247-bp segment of chromosomal DNA on the multicopy broad-host-range vector pRO1614. The presence of this plasmid (pPZ196) in strain PAO1 produced the so-called "hexC effect," a two- to ninefold increase in the activities of four carbohydrate catabolism e...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.15.4700-4709.1994

    authors: Temple L,Sage A,Christie GE,Phibbs PV Jr

    更新日期:1994-08-01 00:00:00

  • Apical vesicles in growing bud cells of heterobasidiomycetous yeasts.

    abstract::Clusters of cytoplasmic vesicles resembling those in growing hyphal apices of mycelial fungi are found near the tips of buds in three heterobasidiomycetous yeasts, Rhodotorula glutinis, Candida scottii, and Sporobolomyces salmonicolor. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.109.2.922-926.1972

    authors: McCully EK,Bracker CE

    更新日期:1972-02-01 00:00:00