Abstract:
:The sequential morphological events in spore formation by Clostridium perfringens type D were observed in Ellner's medium where 80 to 100% of the cells formed spores. Gross structural changes were studied with the light microscope under phase-contrast, and in fixed cells by the use of both nigrosin and Giemsa preparations. Fine structure was examined with the electron microscope in both thin sections and frozen-etched preparations. During the first 3 hr of incubation, the original rod-shaped cells became ellipsoid to ovoid in shape; by 5 to 6 hr, subterminal spores had developed within these enlarged cells. The fine structural sequence was in most respects identical to that in other Bacillaceae, although some stages were illustrated with particular clarity. A unique feature was the development of a convoluted, membranous exosporium which adhered to the outer surface of the two coats and had an unusual fine structure resembling a rectangular array of subunits.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Hoeniger JF,Stuart PF,Holt SCdoi
10.1128/JB.96.5.1818-1834.1968subject
Has Abstractpub_date
1968-11-01 00:00:00pages
1818-34issue
5eissn
0021-9193issn
1098-5530journal_volume
96pub_type
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journal_title:Journal of bacteriology
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pub_type: 杂志文章
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pub_type: 杂志文章
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