Induction of manganese-containing superoxide dismutase is required for acid tolerance in Vibrio vulnificus.

Abstract:

:The manganese-containing superoxide dismutase (MnSOD) of Vibrio vulnificus, normally detected after the onset of the stationary phase, is expressed during the lag that immediately follows the transfer of cells grown exponentially to a fresh medium acidified to pH 5.0, whereas Fe-containing SOD is constitutively expressed. The signal triggering the growth lag and MnSOD induction therein is not low pH but intracellular superoxide accumulated under these conditions, since addition of a superoxide scavenger not only shortened the lag but also abrogated the MnSOD induction. If the lysine decarboxylase reaction proceeds in the presence of sufficient lysine, the broth is rapidly neutralized to abolish the generation of oxidative stress. Accordingly, the acid tolerance response was examined without the addition of lysine. SoxR regulates MnSOD induction. Lack of MnSOD caused by mutations in soxR or sodA resulted in low tolerance to low pH. The fur mutant derepressing MnSOD showed better tolerance than the wild type. Thus, an increase in total cytosolic SOD activity through MnSOD induction is essential for the cell to withstand the acid challenge. The contribution of cuprozinc-containing SOD to acid tolerance is not significant compared with those of cytosolic SODs.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Kim JS,Sung MH,Kho DH,Lee JK

doi

10.1128/JB.187.17.5984-5995.2005

keywords:

subject

Has Abstract

pub_date

2005-09-01 00:00:00

pages

5984-95

issue

17

eissn

0021-9193

issn

1098-5530

pii

187/17/5984

journal_volume

187

pub_type

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