Genetic characterization of the nucleotide excision repair system of Neisseria gonorrhoeae.

Abstract:

:Nucleotide excision repair (NER) is universally used to recognize and remove many types of DNA damage. In eubacteria, the NER system typically consists of UvrA, UvrB, UvrC, the UvrD helicase, DNA polymerase I, and ligase. In addition, when DNA damage blocks transcription, transcription-repair coupling factor (TRCF), the product of the mfd gene, recruits the Uvr complex to repair the damage. Previous work using selected mutants and assays have indicated that pathogenic Neisseria spp. carry a functional NER system. In order to comprehensively examine the role of NER in Neisseria gonorrhoeae DNA recombination and repair processes, the predicted NER genes (uvrA, uvrB, uvrC, uvrD, and mfd) were each disrupted by a transposon insertion, and the uvrB and uvrD mutants were complemented with a copy of each gene in an ectopic locus. Each uvr mutant strain was highly sensitive to UV irradiation and also showed sensitivity to hydrogen peroxide killing, confirming that all of the NER genes in N. gonorrhoeae are functional. The effect of RecA expression on UV survival was minor in uvr mutants but much larger in the mfd mutant. All of the NER mutants demonstrated wild-type levels of pilin antigenic variation and DNA transformation. However, the uvrD mutant exhibited higher frequencies of PilC-mediated pilus phase variation and spontaneous mutation, a finding consistent with a role for UvrD in mismatch repair. We conclude that NER functions are conserved in N. gonorrhoeae and are important for the DNA repair capabilities of this strict human pathogen.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

LeCuyer BE,Criss AK,Seifert HS

doi

10.1128/JB.01018-09

subject

Has Abstract

pub_date

2010-02-01 00:00:00

pages

665-73

issue

3

eissn

0021-9193

issn

1098-5530

pii

JB.01018-09

journal_volume

192

pub_type

杂志文章
  • Role of CodY in regulation of the Bacillus subtilis hut operon.

    abstract::Bacillus subtilis mutants deficient in amino acid repression of the histidine utilization (hut) operon were isolated by transposon mutagenesis. Genetic characterization of these mutants indicated that they most likely contained transposon insertions within the codVWXY operon. The codY gene is required for nutritional ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.13.3779-3784.1996

    authors: Fisher SH,Rohrer K,Ferson AE

    更新日期:1996-07-01 00:00:00

  • Ultrastructure of Azotobacter vinelandii.

    abstract::Vegetative cells and cysts of Azotobacter vinelandii 12837 were prepared for electron microscopy by several methods assumed to preserve structural details destroyed by techniques previously reported in the literature. Examination of large numbers of cells and cysts by these methods revealed four structural details not...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.2.933-939.1970

    authors: Vela GR,Cagle GD,Holmgren PR

    更新日期:1970-11-01 00:00:00

  • Repressor gene finO in plasmids R100 and F: constitutive transfer of plasmid F is caused by insertion of IS3 into F finO.

    abstract::Fertility factor F confers bacterial conjugation, a process which involves at least 20 tra genes. Resistance plasmids such as R100, R6-5, and R1 have homology with F in the tra region. Conjugal transfer of these plasmids is, however, repressed, while transfer of F is constitutive. Repression of R transfer is due to th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.2.619-623.1987

    authors: Yoshioka Y,Ohtsubo H,Ohtsubo E

    更新日期:1987-02-01 00:00:00

  • The linker region of AraC protein.

    abstract::AraC protein, a transcriptional regulator of the L-arabinose operon in Escherichia coli, is dimeric. Each monomer consists of a domain for DNA binding plus transcription activation and a domain for dimerization plus arabinose binding. These are connected to one another by a linker region of at least 5 amino acids. Her...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.24.7025-7030.1996

    authors: Eustance RJ,Schleif RF

    更新日期:1996-12-01 00:00:00

  • A novel deaminase involved in chloronitrobenzene and nitrobenzene degradation with Comamonas sp. strain CNB-1.

    abstract::Comamonas sp. strain CNB-1 degrades nitrobenzene and chloronitrobenzene via the intermediates 2-aminomuconate and 2-amino-5-chloromuconate, respectively. Deamination of these two compounds results in the release of ammonia, which is used as a source of nitrogen for bacterial growth. In this study, a novel deaminase wa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01762-06

    authors: Liu L,Wu JF,Ma YF,Wang SY,Zhao GP,Liu SJ

    更新日期:2007-04-01 00:00:00

  • A bifunctional enzyme in Pseudomonas aeruginosa: a new pattern in the organization of enzymes concerned with phenylalanine and tyrosine biosynthesis.

    abstract::Two isozymes of chorismate mutase (CA mutase(1) and CA mutase(2)) and two isozymes of prephenate dehydratase (PPA dehydratase(1) and PPA dehydratase(2)) have been found in Pseudomonas aeruginosa. The activities CA mutase(2)-PPA dehydratase(2) catalyzing phenylalanine biosynthesis have been purified almost 40-fold and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.115.1.205-212.1973

    authors: Ahmed SI,Campbell JJ

    更新日期:1973-07-01 00:00:00

  • Genomic interrogation of the dassie bacillus reveals it as a unique RD1 mutant within the Mycobacterium tuberculosis complex.

    abstract::Despite their remarkable genetic homology, members of the Mycobacterium tuberculosis complex express very different phenotypes, most notably in their spectra of clinical presentation. For example, M. tuberculosis is regarded as pathogenic to humans, whereas members having deleted RD1, such as Mycobacterium microti and...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.1.104-109.2003

    authors: Mostowy S,Cousins D,Behr MA

    更新日期:2004-01-01 00:00:00

  • Bradyrhizobium japonicum glnB, a putative nitrogen-regulatory gene, is regulated by NtrC at tandem promoters.

    abstract::The glnB gene from Bradyrhizobium japonicum, the endosymbiont of soybeans (Glycine max), was isolated and sequenced, and its expression was examined under various culture conditions and in soybean nodules. The B. japonicum glnB gene encodes a 12,237-dalton polypeptide that is highly homologous to the glnB gene product...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.10.5638-5645.1989

    authors: Martin GB,Thomashow MF,Chelm BK

    更新日期:1989-10-01 00:00:00

  • Genomic rearrangements leading to overexpression of aldo-keto reductase YafB of Escherichia coli confer resistance to glyoxal.

    abstract::Glyoxal is toxic and mutagenic α-oxoaldehyde generated in vivo as an oxidation by-product of sugar metabolism. We selected glyoxal-resistant mutants from an Escherichia coli strain lacking major glyoxal-detoxifying genes, gloA and yqhD, by growing cells in medium containing a lethal concentration of glyoxal. The mutan...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06062-11

    authors: Kwon M,Lee J,Lee C,Park C

    更新日期:2012-04-01 00:00:00

  • The ToxR protein of Vibrio cholerae forms homodimers and heterodimers.

    abstract::The ToxR protein of Vibrio cholerae regulates the expression of several virulence factors that play important roles in the pathogenesis of cholera. Previous experiments with ToxR-alkaline phosphatase (ToxR-PhoA) fusion proteins suggested a model for gene regulation in which the inactive form of ToxR was a monomer and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.1.156-162.1996

    authors: Ottemann KM,Mekalanos JJ

    更新日期:1996-01-01 00:00:00

  • 2-keto-3-deoxygluconate, an intermediate in the fermentation of gluconate by clostridia.

    abstract::Five clostridial species were found to ferment gluconate via 2-keto-3-deoxygluconate which subsequently is phosphorylated to yield 2-keto-3-deoxy-6-phosphogluconate (KDPG). This compound is then cleaved by KDPG aldolase. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.107.2.570-573.1971

    authors: Bender R,Andreesen JR,Gottschalk G

    更新日期:1971-08-01 00:00:00

  • Metal ion content of Escherichia coli versus cell age.

    abstract::The potassium, calcium, magnesium, and zinc ion content of cells in exponential and synchronously growing cultures of Escherichia coli B/r was determined with an X-ray fluorescence spectrometer and an atomic absorption spectrophotometer. Cellular potassium, calcium, and magnesium content increased smoothly during the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.126.3.1089-1095.1976

    authors: Kung FC,Raymond J,Glaser DA

    更新日期:1976-06-01 00:00:00

  • ColE1 plasmid incompatibility: localization and analysis of mutations affecting incompatibility.

    abstract::Deletion mutants of plasmid ColE1 that involve the replication origin and adjacent regions of the plasmid have been studied to determine the mechanism by which those mutations affect the expression of plasmid incompatibility. It was observed that (i) a region of ColE1 that is involved in the expression of plasmid inco...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.139.2.608-619.1979

    authors: Hashimoto-Gotoh T,Inselburg J

    更新日期:1979-08-01 00:00:00

  • Suppression of insertions in the complex pdxJ operon of Escherichia coli K-12 by lon and other mutations.

    abstract::Complementation analyses using minimal recombinant clones showed that all known pdx point mutations, which cause pyridoxine (vitamin B6) or pyridoxal auxotrophy, are located in the pdxA, pdxB, serC, pdxJ, and pdxH genes. Antibiotic enrichments for chromosomal transposon mutants that require pyridoxine (vitamin B6) or ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.5.1554-1567.1992

    authors: Lam HM,Tancula E,Dempsey WB,Winkler ME

    更新日期:1992-03-01 00:00:00

  • Comparative specificities of two evolutionarily divergent hydrolases involved in microbial degradation of polychlorinated biphenyls.

    abstract::2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoate (HOPDA) hydrolase (BphD) is a key determinant in the aerobic transformation of polychlorinated biphenyls (PCBs) by Burkholderia sp. strain LB400 (S. Y. K. Seah, G. Labbé, S. Nerdinger, M. Johnson, V. Snieckus, and L. D. Eltis, J. Biol. Chem. 275:15701-15708, 2000). To determin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.5.1511-1516.2001

    authors: Seah SY,Labbé G,Kaschabek SR,Reifenrath F,Reineke W,Eltis LD

    更新日期:2001-03-01 00:00:00

  • 3-Hydroxypropionyl-coenzyme A synthetase from Metallosphaera sedula, an enzyme involved in autotrophic CO2 fixation.

    abstract::A modified 3-hydroxypropionate cycle has been proposed as the autotrophic CO2 fixation pathway for the thermoacidophilic crenarchaeon Metallosphaera sedula. The cycle requires the reductive conversion of 3-hydroxypropionate to propionyl-coenzyme A (propionyl-CoA). The specific activity of the 3-hydroxypropionate-, CoA...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01593-07

    authors: Alber BE,Kung JW,Fuchs G

    更新日期:2008-02-01 00:00:00

  • Inverted repeats in the DNA of plasmid pCU1.

    abstract::Renaturable regions in the DNA strands of the N group plasmid pCU1 have been visualized as stem-loop structures by electron microscopy. Four such distinct structures are described, the smallest of which is within the loop of a larger one. The region of pCU1 in which these structures occur has several restriction sites...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.153.3.1502-1512.1983

    authors: Konarska-Kozlowska M,Thatte V,Iyer VN

    更新日期:1983-03-01 00:00:00

  • Two homologous genes coding for spore-specific proteins are expressed at different times during development of Myxococcus xanthus.

    abstract::The ops and tps genes of Myxococcus xanthus have ca. 90% DNA and amino acid sequence homology and are in the same orientation separated by a spacer region of only 1.4 kilobases. The products of the two genes were found to cross-react immunologically, and both were capable of Ca2+-dependent self-assembly on the surface...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.163.1.121-125.1985

    authors: Teintze M,Thomas R,Furuichi T,Inouye M,Inouye S

    更新日期:1985-07-01 00:00:00

  • Brucella-Salmonella lipopolysaccharide chimeras are less permeable to hydrophobic probes and more sensitive to cationic peptides and EDTA than are their native Brucella sp. counterparts.

    abstract::A rough (R) Brucella abortus 45/20 mutant was more sensitive to the bactericidal activity of polymyxin B and lactoferricin B than was its smooth (S) counterpart but considerably more resistant than Salmonella montevideo. The outer membrane (OM) and isolated lipopolysaccharide (LPS) of S. montevideo showed a higher aff...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.20.5867-5876.1996

    authors: Freer E,Moreno E,Moriyón I,Pizarro-Cerdá J,Weintraub A,Gorvel JP

    更新日期:1996-10-01 00:00:00

  • Relation of catalase to substrate utilization by Mycoplasma pneumoniae.

    abstract::No catalase activity was detected in four strains of glucose-grown Mycoplasma pneumoniae at any time during the replication of the organism. Exogenous catalase dramatically increased the O(2) uptake with glycerol, presumably by releasing inhibition caused by hydrogen peroxide. The effect of added catalase on the O(2) ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.4.1425-1430.1968

    authors: Low IE,Eaton MD,Proctor P

    更新日期:1968-04-01 00:00:00

  • Cloning and characterization of the Salmonella typhimurium metE gene.

    abstract::The metE gene of Salmonella typhimurium was cloned into plasmid pACYC184 by using a lambda gt7 -metE transducing phage as a source of metE DNA. The recombinant plasmid, designated pGS41 , carries a 12.8-kilobase-pair EcoRI insert fragment. The metE gene was subcloned from pGS41 into plasmid pBR322 on a 4.2-kilobase-pa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.158.3.928-933.1984

    authors: Schulte LL,Stauffer LT,Stauffer GV

    更新日期:1984-06-01 00:00:00

  • Core and panmetabolism in Escherichia coli.

    abstract::Escherichia coli exhibits a wide range of lifestyles encompassing commensalism and various pathogenic behaviors which its highly dynamic genome contributes to develop. How environmental and host factors shape the genetic structure of E. coli strains remains, however, largely unknown. Following a previous study of E. c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01192-10

    authors: Vieira G,Sabarly V,Bourguignon PY,Durot M,Le Fèvre F,Mornico D,Vallenet D,Bouvet O,Denamur E,Schachter V,Médigue C

    更新日期:2011-03-01 00:00:00

  • Functional characterization of seven γ-Glutamylpolyamine synthetase genes and the bauRABCD locus for polyamine and β-Alanine utilization in Pseudomonas aeruginosa PAO1.

    abstract::Pseudomonas aeruginosa and many other bacteria can utilize biogenic polyamines, including diaminopropane (DAP), putrescine (Put), cadaverine (Cad), and spermidine (Spd), as carbon and/or nitrogen sources. Transcriptome analysis in response to exogenous Put and Spd led to the identification of a list of genes encoding ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05105-11

    authors: Yao X,He W,Lu CD

    更新日期:2011-08-01 00:00:00

  • Possible involvement of the division cycle in dispersal of Escherichia coli from biofilms.

    abstract::Growth rate control of adherent, sessile populations was achieved by the controlled perfusion of membrane-associated bacterial biofilms by the method of Gilbert et al. (P. Gilbert, D. G. Allison, D. J. Evans, P. S. Handley, and M. R. W. Brown, Appl. Environ. Microbiol. 55:1308-1311, 1989). Changes in cell surface hydr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.3.1667-1669.1990

    authors: Allison DG,Evans DJ,Brown MR,Gilbert P

    更新日期:1990-03-01 00:00:00

  • AfsK-Mediated Site-Specific Phosphorylation Regulates DnaA Initiator Protein Activity in Streptomyces coelicolor.

    abstract::In all organisms, chromosome replication is regulated mainly at the initiation step. Most of the knowledge about the mechanisms that regulate replication initiation in bacteria has come from studies on rod-shaped bacteria, such as Escherichia coli and Bacillus subtilisStreptomyces is a bacterial genus that is characte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00597-19

    authors: Łebkowski T,Wolański M,Ołdziej S,Flärdh K,Zakrzewska-Czerwińska J

    更新日期:2020-01-15 00:00:00

  • Proteus mirabilis mutants defective in swarmer cell differentiation and multicellular behavior.

    abstract::Proteus mirabilis is a dimorphic bacterium which exists in liquid cultures as a 1.5- to 2.0-microns motile swimmer cell possessing 6 to 10 peritrichous flagella. When swimmer cells are placed on a surface, they differentiate by a combination of events that ultimately produce a swarmer cell. Unlike the swimmer cell, th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.19.6279-6288.1991

    authors: Belas R,Erskine D,Flaherty D

    更新日期:1991-10-01 00:00:00

  • Temperature-sensitive lesions in the Francisella novicida valA gene cloned into an Escherichia coli msbA lpxK mutant affecting deoxycholate resistance and lipopolysaccharide assembly at the restrictive temperature.

    abstract::The valAB locus of Francisella novicida has previously been found to be highly similar at the deduced amino acid level to msbA lpxK of Escherichia coli. Both ValA and MsbA are members of the superfamily of ABC transporters, and they appear to have similar functions. In this study we describe the isolation of a tempera...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.24.7638-7643.1997

    authors: McDonald MK,Cowley SC,Nano FE

    更新日期:1997-12-01 00:00:00

  • Positive regulation of the Escherichia coli glycine cleavage enzyme system.

    abstract::A new mutation in Escherichia coli, designated gcvA1, that results in noninducible expression of both gcv and a gcvT-lacZ gene fusion was isolated. A plasmid carrying the wild-type gcvA gene complemented the mutation and restored glycine-inducible gcv and gcvT-lacZ gene expression. These results suggest that gcvA enco...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.3.902-904.1993

    authors: Wilson RL,Steiert PS,Stauffer GV

    更新日期:1993-02-01 00:00:00

  • A third L-proline permease in Salmonella typhimurium which functions in media of elevated osmotic strength.

    abstract::Exogenous proline specifically stimulates the growth rate of enteric bacteria in media of inhibitory osmotic strength (J. H. B. Christian, Aust. J. Biol. Sci. 8:490-497, 1955). I observed that Salmonella typhimurium mutants which lack both of the previously known proline permeases (putP proP) are stimulated by proline...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.151.3.1433-1443.1982

    authors: Csonka LN

    更新日期:1982-09-01 00:00:00

  • Ultrastructural localization of carbohydrates on thin sections of Staphylococcus aureus with silver methenamine and wheat germ agglutinin-gold complex.

    abstract::Postembedding staining of intracellular carbohydrates on thin sections of Staphylococcus aureus was studied by the silver methenamine and the wheat germ agglutinin-gold techniques. Staining of silver grains was observed on both the cell wall and the cross wall. The staining was interpreted to be due to teichoic acid. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.3.1358-1362.1987

    authors: Morioka H,Tachibana M,Suganuma A

    更新日期:1987-03-01 00:00:00