Abstract:
:Nucleotide excision repair (NER) is universally used to recognize and remove many types of DNA damage. In eubacteria, the NER system typically consists of UvrA, UvrB, UvrC, the UvrD helicase, DNA polymerase I, and ligase. In addition, when DNA damage blocks transcription, transcription-repair coupling factor (TRCF), the product of the mfd gene, recruits the Uvr complex to repair the damage. Previous work using selected mutants and assays have indicated that pathogenic Neisseria spp. carry a functional NER system. In order to comprehensively examine the role of NER in Neisseria gonorrhoeae DNA recombination and repair processes, the predicted NER genes (uvrA, uvrB, uvrC, uvrD, and mfd) were each disrupted by a transposon insertion, and the uvrB and uvrD mutants were complemented with a copy of each gene in an ectopic locus. Each uvr mutant strain was highly sensitive to UV irradiation and also showed sensitivity to hydrogen peroxide killing, confirming that all of the NER genes in N. gonorrhoeae are functional. The effect of RecA expression on UV survival was minor in uvr mutants but much larger in the mfd mutant. All of the NER mutants demonstrated wild-type levels of pilin antigenic variation and DNA transformation. However, the uvrD mutant exhibited higher frequencies of PilC-mediated pilus phase variation and spontaneous mutation, a finding consistent with a role for UvrD in mismatch repair. We conclude that NER functions are conserved in N. gonorrhoeae and are important for the DNA repair capabilities of this strict human pathogen.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
LeCuyer BE,Criss AK,Seifert HSdoi
10.1128/JB.01018-09subject
Has Abstractpub_date
2010-02-01 00:00:00pages
665-73issue
3eissn
0021-9193issn
1098-5530pii
JB.01018-09journal_volume
192pub_type
杂志文章abstract::Bacillus subtilis mutants deficient in amino acid repression of the histidine utilization (hut) operon were isolated by transposon mutagenesis. Genetic characterization of these mutants indicated that they most likely contained transposon insertions within the codVWXY operon. The codY gene is required for nutritional ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.13.3779-3784.1996
更新日期:1996-07-01 00:00:00
abstract::Vegetative cells and cysts of Azotobacter vinelandii 12837 were prepared for electron microscopy by several methods assumed to preserve structural details destroyed by techniques previously reported in the literature. Examination of large numbers of cells and cysts by these methods revealed four structural details not...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.2.933-939.1970
更新日期:1970-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1987-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.24.7025-7030.1996
更新日期:1996-12-01 00:00:00
abstract::Comamonas sp. strain CNB-1 degrades nitrobenzene and chloronitrobenzene via the intermediates 2-aminomuconate and 2-amino-5-chloromuconate, respectively. Deamination of these two compounds results in the release of ammonia, which is used as a source of nitrogen for bacterial growth. In this study, a novel deaminase wa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01762-06
更新日期:2007-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1973-07-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2004-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.10.5638-5645.1989
更新日期:1989-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2012-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.1.156-162.1996
更新日期:1996-01-01 00:00:00
abstract::Five clostridial species were found to ferment gluconate via 2-keto-3-deoxygluconate which subsequently is phosphorylated to yield 2-keto-3-deoxy-6-phosphogluconate (KDPG). This compound is then cleaved by KDPG aldolase. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.107.2.570-573.1971
更新日期:1971-08-01 00:00:00
abstract::The potassium, calcium, magnesium, and zinc ion content of cells in exponential and synchronously growing cultures of Escherichia coli B/r was determined with an X-ray fluorescence spectrometer and an atomic absorption spectrophotometer. Cellular potassium, calcium, and magnesium content increased smoothly during the ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.126.3.1089-1095.1976
更新日期:1976-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.2.608-619.1979
更新日期:1979-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.174.5.1554-1567.1992
更新日期:1992-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.183.5.1511-1516.2001
更新日期:2001-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2008-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.3.1502-1512.1983
更新日期:1983-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1985-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1996-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.4.1425-1430.1968
更新日期:1968-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.3.928-933.1984
更新日期:1984-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01192-10
更新日期:2011-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.3.1667-1669.1990
更新日期:1990-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2020-01-15 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.19.6279-6288.1991
更新日期:1991-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.24.7638-7643.1997
更新日期:1997-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.3.902-904.1993
更新日期:1993-02-01 00:00:00
abstract::Exogenous proline specifically stimulates the growth rate of enteric bacteria in media of inhibitory osmotic strength (J. H. B. Christian, Aust. J. Biol. Sci. 8:490-497, 1955). I observed that Salmonella typhimurium mutants which lack both of the previously known proline permeases (putP proP) are stimulated by proline...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.3.1433-1443.1982
更新日期:1982-09-01 00:00:00
abstract::Postembedding staining of intracellular carbohydrates on thin sections of Staphylococcus aureus was studied by the silver methenamine and the wheat germ agglutinin-gold techniques. Staining of silver grains was observed on both the cell wall and the cross wall. The staining was interpreted to be due to teichoic acid. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.3.1358-1362.1987
更新日期:1987-03-01 00:00:00