The linker region of AraC protein.

Abstract:

:AraC protein, a transcriptional regulator of the L-arabinose operon in Escherichia coli, is dimeric. Each monomer consists of a domain for DNA binding plus transcription activation and a domain for dimerization plus arabinose binding. These are connected to one another by a linker region of at least 5 amino acids. Here we have addressed the question of whether any of the amino acids in the linker region play active, specific, and crucial structural roles or whether these amino acids merely serve as passive spacers between the functional domains. We found that all but one of the linker amino acids can be changed to other amino acids individually and in small groups without substantially affecting the ability of AraC protein to activate transcription when arabinose is present. When, however, the entire linker region is replaced with linker sequences from other proteins, the functioning of AraC is impaired.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Eustance RJ,Schleif RF

doi

10.1128/jb.178.24.7025-7030.1996

subject

Has Abstract

pub_date

1996-12-01 00:00:00

pages

7025-30

issue

24

eissn

0021-9193

issn

1098-5530

journal_volume

178

pub_type

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