Fractionation of Newcastle disease virus by chromatography on diethylaminoethyl cellulose.

Abstract:

:Wilson, Dwight E. (Rensselaer Polytechnic Institute, Troy, N.Y.). Fractionation of Newcastle disease virus by chromatography on diethylaminoethyl cellulose. J. Bacteriol. 84:295-301. 1962.-The L. Kansas and NK strains of Newcastle disease virus were chromatographed on diethylaminoethyl (DEAE) cellulose ion-exchange columns. L. Kansas virus eluted from DEAE columns showed one peak of hemagglutinating and infective particles. Two peaks of hemagglutinins and one peak of infective particles were observed when the NK strain was chromatographed, indicating that the stock virus contained a noninfectious hemagglutinating component. Treatment of the noninfectious particles with Genetron 113 resulted in an increase in the ratio of infective to hemagglutinating particles. The Genetron-treated noninfectious particles were also eluted from the DEAE column at a higher salt concentration than the untreated noninfectious particles. The results indicate that the Genetron treatment removes inhibitors of infectivity bound to the noninfectious virus particle.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

WILSON DE

doi

10.1128/JB.84.2.295-301.1962

keywords:

subject

Has Abstract

pub_date

1962-08-01 00:00:00

pages

295-301

eissn

0021-9193

issn

1098-5530

journal_volume

84

pub_type

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