Role of CodY in regulation of the Bacillus subtilis hut operon.

Abstract:

:Bacillus subtilis mutants deficient in amino acid repression of the histidine utilization (hut) operon were isolated by transposon mutagenesis. Genetic characterization of these mutants indicated that they most likely contained transposon insertions within the codVWXY operon. The codY gene is required for nutritional regulation of the dipeptide permease (dpp) operon. An examination of hut expression in a delta codY mutant demonstrated that amino acid repression exerted at the hutOA operator, which lies immediately downstream of the hut promoter, was defective in a delta codY mutant. The codY gene product was not required for amino acid regulation of either hut induction or the expression of proline oxidase, the first enzyme in proline degradation. This indicates that more than one mechanism of amino acid repression is present in B. subtilis. An examination of dpp and hut expression in cells during exponential growth in various media revealed that the level of CodY-dependent regulation appeared to be related to the growth rate of the culture.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Fisher SH,Rohrer K,Ferson AE

doi

10.1128/jb.178.13.3779-3784.1996

subject

Has Abstract

pub_date

1996-07-01 00:00:00

pages

3779-84

issue

13

eissn

0021-9193

issn

1098-5530

journal_volume

178

pub_type

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