Abstract:
:The recF gene of Escherichia coli is known to encode an Mr-40,000 protein that is involved in DNA recombinationa nd postreplication DNA repair. To characterize the role of the recF gene product in these processes, the recF gene was cloned downstream of a tac promoter to facilitate overproduction of the recF gene product. The RecF protein was overproduced and purified to apparent homogeneity. N-terminal protein sequence analysis demonstrated that the purified protein had the sequence that was predicted from the DNA sequence of the recF gene, except that the predicted N-terminal Met was not present. The RecF protein bound to single-stranded oligonucleotides in filter binding and gel filtration assays. Maximal binding required 2 to 3 min of incubation at 37 degrees C; the binding reaction had a pH optimum of 7.0, did not require divalent cations, and was inhibited by NaCl concentrations of greater than 250 mM. The Kd of RecF protein binding to a 59-base single-stranded oligonucleotide was on the order of 1.3 X 10(-7) M, and the reaction did not show cooperativity. Experiments measuring the binding to various DNA substrates and competition binding experiments with different DNA molecules demonstrated that RecF protein binds preferentially to single-stranded, linear DNA molecules.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Griffin TJ 4th,Kolodner RDdoi
10.1128/jb.172.11.6291-6299.1990subject
Has Abstractpub_date
1990-11-01 00:00:00pages
6291-9issue
11eissn
0021-9193issn
1098-5530journal_volume
172pub_type
杂志文章abstract::Methicillin resistance in staphylococci is mediated by PBP2a, a penicillin binding protein with low affinity for beta-lactam antibiotics. The gene encoding PBP2a, mecA, is transcriptionally regulated in some clinical isolates by mecR1 and mecI, genes divergently transcribed from mecA that encode a signal transducer an...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.8.2160-2166.1998
更新日期:1998-04-01 00:00:00
abstract::Lysozyme is an important component of the innate immune system. It functions by hydrolyzing the peptidoglycan (PG) layer of bacteria. The human pathogen Listeria monocytogenes is intrinsically lysozyme resistant. The peptidoglycan N-deacetylase PgdA and O-acetyltransferase OatA are two known factors contributing to it...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00553-20
更新日期:2021-01-25 00:00:00
abstract::The bdr (Borrelia direct repeat) gene family of the genus Borrelia encodes a polymorphic group of proteins that carry a central repeat motif region containing putative phosphorylation sites and a hydrophobic carboxyl-terminal domain. It has been postulated that the Bdr proteins may anchor to the inner membrane via the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.15.4222-4226.2000
更新日期:2000-08-01 00:00:00
abstract::The Escherichia coli gene coding for dihydropteroate synthase (DHPS) has been cloned and sequenced. The protein has 282 amino acids and a compositional molecular mass of 30,314 daltons. Increased expression of the enzyme was realized by using a T7 expression system. The enzyme was purified and crystallized. A temperat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.18.5961-5970.1992
更新日期:1992-09-01 00:00:00
abstract::PCR-based site-directed mutagenesis has been used to generate 38 alanine-substitution mutations in the C-terminal 41 amino acid residues of LuxR. This region plays a critical role in the mechanism of LuxR-dependent transcriptional activation of the Vibrio fischeri lux operon during quorum sensing. The ability of the v...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.1.387-392.2001
更新日期:2001-01-01 00:00:00
abstract::ZmaR is a resistance determinant of unusual abundance in the environment and confers on gram-positive and gram-negative bacteria resistance to zwittermicin A, a novel broad-spectrum antibiotic produced by species of Bacillus. The ZmaR protein has no sequence similarity to proteins of known function; thus, the purpose ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.17.5455-5460.1999
更新日期:1999-09-01 00:00:00
abstract::A lytic enzyme active against viable, intact staphylococci is released into culture fluids upon lysis of bacteriophage-infected Staphylococcus aureus PS53 cells. This enzyme, staphylococcal phage-associated lysin (PAL), was partially purified by ammonium sulfate precipitation and gel filtration through Sephadex G-200....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.107.2.499-504.1971
更新日期:1971-08-01 00:00:00
abstract::Erythrobacter litoralis has been known as a bacteriochlorophyll a-containing, aerobic, anoxygenic, phototrophic bacterium. Here we announce the complete genome sequence of E. litoralis HTCC2594, which is devoid of phototrophic potential. E. litoralis HTCC2594, isolated by dilution-to-extinction culturing from seawater...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00026-09
更新日期:2009-04-01 00:00:00
abstract::Bacteria within the Mycobacterium avium complex are prominent in the environment and are a source of serious disseminated infections in patients with AIDS. Serovars of the M. avium complex are distinguished from all other mycobacteria and from one another by the presence of highly antigenic glycolipids, the glycopepti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.21.6991-6997.1991
更新日期:1991-11-01 00:00:00
abstract::There are two closely related hik31 operons involved in signal transduction on the chromosome and the pSYSX plasmid in the cyanobacterium Synechocystis sp. strain PCC 6803. We studied the growth, cell morphology, and gene expression in operon and hik mutants for both copies, under different growth conditions, to exami...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06207-11
更新日期:2012-01-01 00:00:00
abstract::cat-86 is a promoter-deficient plasmid gene that encodes chloramphenicol acetyltransferase (CAT). Insertion of a promoter at a site 144 base pairs 5' to the cat-86 coding sequence activates transcription of the gene and allows cat-86 to specify chloramphenicol-inducible CAT activity in Bacillus subtilis. Induction of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.3.967-972.1987
更新日期:1987-03-01 00:00:00
abstract::Peptide inhibitors of phage lambda site-specific recombination were previously isolated by screening synthetic combinatorial peptide libraries. These inhibitors cause the accumulation of complexes between the recombinase and the Holliday junction intermediate of several highly divergent tyrosine recombinases. Peptide ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01559-08
更新日期:2009-04-01 00:00:00
abstract::Bacillus brevis HPD31 contains a surface (S)-layer protein, termed the HWP, which forms a hexagonal array in the cell wall. The 5' region of the HWP gene was isolated from a DNA library constructed in bacteriophage vector EMBL3 from a partial BamHI digest of the chromosomal DNA. The 3' region contained in a 2.7-kiloba...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.3.1312-1320.1990
更新日期:1990-03-01 00:00:00
abstract::The alpha-aminoadipate pathway for the biosynthesis of lysine is present only in fungi and euglena. Until now, this unique metabolic pathway has never been investigated in the opportunistic fungal pathogens Candida albicans, Cryptococcus neoformans, and Aspergillus fumigatus. Five of the eight enzymes (homocitrate syn...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.22.7379-7384.1992
更新日期:1992-11-01 00:00:00
abstract::Bacterial pathogenesis depends on changes in metabolic and virulence gene expression in response to changes within a pathogen's environment. The plague-causing pathogen, Yersinia pestis, requires expression of the gene encoding the Pla protease for progression of pneumonic plague. The catabolite repressor protein Crp,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00737-17
更新日期:2018-05-09 00:00:00
abstract::The guanine plus cytosine (GC) content of the deoxyribonucleic acid (DNA) of 11 isolates of host-dependent (H-D) bdellovibrios and 18 host-independent (H-I) derivatives was determined from thermal denaturation curves and buoyant densities in CsCl. The H-D and respective H-I cultures have GC contents which are identica...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.2.786-790.1969
更新日期:1969-11-01 00:00:00
abstract::In acetate-limited chemostat cultures started with single-colony cultures of Thiobacillus versutus, a mutant appeared after approximately 85 volume changes. The inhomogeneity of the culture was detected by the development of two different types of colonies on agar plates. When a pure culture of the mutant was grown in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.2.791-794.1986
更新日期:1986-11-01 00:00:00
abstract::Feldman, L. A. (Baylor University College of Medicine, Houston, Tex.), J. L. Melnick, and F. Rapp. Influence of SV40 genome on the replication of an adenovirus-SV40 "hybrid" population. J. Bacteriol. 90:778-782. 1965.-Replication of a type 7 adenovirus-SV40 hybrid population in primary African green monkey kidney cell...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.3.778-782.1965
更新日期:1965-09-01 00:00:00
abstract::Escherichia coli WP2 bacteria with an ochre amino acid auxotrophy show no evidence of growth during the first few days after plating at densities above 10(8) on plates lacking the required amino acid. They lose viability for some days, and then a subpopulation recovers and there is cell turnover. At very low plating d...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.11.2906-2910.1998
更新日期:1998-06-01 00:00:00
abstract::The 12 incD repeats in the F plasmid each contain about 60 bits of information, which is three times the amount of conservation that a single protein would need to distinguish the repeats from the rest of the Escherichia coli genome. This is the first reported discovery of a case of threefold excess information, and i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.11.3558-3560.1992
更新日期:1992-06-01 00:00:00
abstract::Ferric reductase activity was examined in Azotobacter vinelandii and was found to be located in the cytoplasm. The specific activities of soluble cell extracts were not affected by the iron concentration of the growth medium; however, activity was inhibited by the presence of Zn2+ during cell growth and also by the ad...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.7.4031-4037.1989
更新日期:1989-07-01 00:00:00
abstract::The product of the papD gene of uropathogenic Escherichia coli is required for the biogenesis of digalactoside-binding P pili. Mutations within papD result in complete degradation of the major pilus subunit, PapA, and of the pilinlike proteins PapE and PapF and also cause partial breakdown of the PapG adhesin. The pap...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.11.6052-6058.1989
更新日期:1989-11-01 00:00:00
abstract::Several resistant mutants of a transformable group H streptococcus, strain Challis, were isolated from media containing high concentrations of streptomycin. Mutants SR5a and SR5 exhibited high and low transformability, respectively, when exposed to deoxyribonucleic acid (DNA) from a novobiocin-resistant Challis strain...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.1.132-138.1968
更新日期:1968-01-01 00:00:00
abstract::A strain of Pseudomonas (P. putida HS1) was found to resemble P. putida (arvilla) mt-2 in its ability to degrade toluene, m- and p-xylene, 1,2,4-trimethylbenzene (pseudocumene), and 3-ethyltoluene via oxidation of a methyl substituent and reactions of the meta-fission pathway. The ability to degrade these substrates b...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.146.3.952-964.1981
更新日期:1981-06-01 00:00:00
abstract::The weak organic acid sorbic acid is a commonly used food preservative, as it inhibits the growth of bacteria, yeasts, and molds. We have used genome-wide transcriptional profiling of Bacillus subtilis cells during mild sorbic acid stress to reveal the growth-inhibitory activity of this preservative and to identify po...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01516-07
更新日期:2008-03-01 00:00:00
abstract::sigma E is a sporulation-specific sigma factor of Bacillus subtilis that is formed from an inactive precursor protein (pro-sigma E) by the removal of 27 to 29 amino acids from the pro-sigma E amino terminus. By using oligonucleotide-directed mutagenesis, sequential deletions were constructed in the precursor-specific ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.14.4629-4637.1992
更新日期:1992-07-01 00:00:00
abstract::The fermentative hyperthermophile Pyrococcus furiosus contains an NADPH-utilizing, heterotetrameric (alphabetagammadelta), cytoplasmic hydrogenase (hydrogenase I) that catalyzes both H(2) production and the reduction of elemental sulfur to H(2)S. Herein is described the purification of a second enzyme of this type, hy...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.7.1864-1871.2000
更新日期:2000-04-01 00:00:00
abstract::Jones, Lois M. (University of Wisconsin, Madison), C. R. McDuff, and J. B. Wilson. Phenotypic alterations in the colonial morphology of Brucella abortus due to a bacteriophage carrier state. J. Bacteriol. 83:860-866. 1962.-In the course of examining a number of Brucella cultures with a brucellaphage, it was observed t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.4.860-866.1962
更新日期:1962-04-01 00:00:00
abstract::Virulence genes in Listeria monocytogenes are coordinately expressed under the control of the transcriptional activator PrfA, encoded by prfA, a member of the cyclic AMP (cAMP) receptor protein (CRP)/FNR family of bacterial regulators. Strain P14-A is a spontaneous mutant of L. monocytogenes serovar 4b which produces ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.5.1533-1540.1997
更新日期:1997-03-01 00:00:00
abstract::We describe the identification and characterization of two genes and their gene products responsible for determining receptor binding and pilus length in type 1-piliated Escherichia coli. One gene, pilE, conferred the ability of piliated cells to agglutinate guinea pig erythrocytes. The other gene, pilF, determined pi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.2.640-645.1987
更新日期:1987-02-01 00:00:00