Abstract:
:Mitospores or cysts of Allomyces macrogynus do not take up the glucose analogue 2-deoxyglucose. Uptake of 2-deoxyglucose by germlings begins at 25 min into germination, the start of the rhizoid stage, and increases in rate by approximately 50-fold until 100 min into germination. The rate remains constant from 100 to 200 min, at which time germination is completed and hyphal formation begins. The presence of glucose in the germination medium blocks the uptake of 2-deoxyglucose. Of the other sugars tested, only galactose had any effect on 2-deoxyglucose uptake. Actinomycin D treatment during germination in a glucose-containing medium prevented the appearance of the uptake system, but actinomycin D was not effective after the transfer to a glucose-free medium. Cycloheximide treatment prevented the appearance of the uptake system if it was added at the time of the transfer to the glucose-free medium; it inhibited uptake only partially if the germlings were starved of glucose before its addition. It appears, therefore, that both ribonucleic acid synthesis during germination and protein synthesis after the removal of glucose are required for the uptake of 2-deoxyglucose.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Burke DDdoi
10.1128/JB.124.2.843-848.1975keywords:
subject
Has Abstractpub_date
1975-11-01 00:00:00pages
843-8issue
2eissn
0021-9193issn
1098-5530journal_volume
124pub_type
杂志文章abstract::Streptococcus bovis possesses two sugar phosphate phosphatases (Pases). Pase I is a soluble enzyme that is inhibited by the membrane fractions from lactose-grown cells and is insensitive to activation by S46D HPr, an analog of HPr(ser-P) of the sugar phosphotransferase system. Pase II is a membrane-associated enzyme t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.23.7007-7009.1995
更新日期:1995-12-01 00:00:00
abstract::Phosphate is an essential nutrient that Staphylococcus aureus and other pathogens must acquire from the host during infection. While inorganic monophosphate (Pi) is the preferred source of this nutrient, bacteria can also obtain it from phosphate-containing organic molecules. The Pi-responsive regulator PhoPR is neces...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00264-20
更新日期:2020-10-22 00:00:00
abstract::Type III secretion is used by many gram-negative bacterial pathogens to directly deliver protein toxins (effectors) into targeted host cells. In all cases, secretion of effectors is triggered by host cell contact, although the mechanism is unclear. In Pseudomonas aeruginosa, expression of all type III secretion-relate...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01553-07
更新日期:2008-04-01 00:00:00
abstract::Pseudomonas fluorescens strain KU-7 is a prototype microorganism that metabolizes 2-nitrobenzoate (2-NBA) via the formation of 3-hydroxyanthranilate (3-HAA), a known antioxidant and reductant. The initial two steps leading to the sequential formation of 2-hydroxy/aminobenzoate and 3-HAA are catalyzed by a NADPH-depend...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01098-06
更新日期:2007-05-01 00:00:00
abstract::The marine bacterium and human pathogen Vibrio parahaemolyticus rapidly colonizes surfaces by using swarming motility and forming robust biofilms. Entering one of the two colonization programs, swarming motility or sessility, involves differential regulation of many genes, resulting in a dramatic shift in physiology a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00723-19
更新日期:2020-02-25 00:00:00
abstract::The major facilitator superfamily includes a large collection of evolutionarily related proteins that have been implicated in the transport of a variety of solutes and metabolites across the membranes of organisms ranging from bacteria to humans. We have recently reported the three-dimensional structure, at 6.5 A reso...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.5.1712-1718.2003
更新日期:2003-03-01 00:00:00
abstract::Surface protein antigens of Mycoplasma hyopneumoniae were identified by direct antibody-surface binding or by radioimmunoprecipitation of surface 125I-labeled proteins with a series of monoclonal antibodies (MAbs). Surface proteins p70, p65, p50, and p44 were shown to be integral membrane components by selective parti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.12.5546-5555.1987
更新日期:1987-12-01 00:00:00
abstract::The expression of the cyanobacterial recA gene, isolated from Anabaena variabilis, has been examined at the levels of transcript and protein abundance. Exposure of the cyanobacterium to a variety of DNA-damaging agents, including mitomycin C, methyl methanesulfonate, and UV irradiation, results in a rapid increase in ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.10.5713-5719.1989
更新日期:1989-10-01 00:00:00
abstract::Azotobacter vinelandii presents a differentiation process leading to the formation of desiccation-resistant cysts. Alginate, the exopolysaccharide produced by this bacterium, has been postulated to have a role in cyst formation. Here, we report the cloning and characterization of the A. vinelandii gene coding for the ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.7.1793-1799.1996
更新日期:1996-04-01 00:00:00
abstract:UNLABELLED:Clostridium perfringens type D strains are usually associated with diseases of livestock, and their virulence requires the production of epsilon toxin (ETX). We previously showed (J. Li, S. Sayeed, S. Robertson, J. Chen, and B. A. McClane, PLoS Pathog 7:e1002429, 2011, http://dx.doi.org/10.1371/journal.ppat....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00349-15
更新日期:2015-10-01 00:00:00
abstract::Deoxyribonucleic acid (DNA) reassociation analyses were employed to determine the molecular relationships between recombinable nocardiae. Analysis of the compatibility system of Nocardia erythropolis Mat-Ce and Mat-cE mating strains demonstrated the existence of extensive homology under both exacting and nonexacting c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.109.2.720-729.1972
更新日期:1972-02-01 00:00:00
abstract::The levels of transfer ribonucleic acids (tRNAs) specific for 14 amino acids were almost identical in dormant spores and in spores germinated from 6 to 75 min. Germinated spore tRNAs specific for all amino acids tested were between 63 and 93% charged, and there was no significant change in this value from 6 to 75 min ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.118.3.1067-1074.1974
更新日期:1974-06-01 00:00:00
abstract::Enteropathogenic Escherichia coli employs a type III secretion system (T3SS) to translocate virulence effector proteins directly into enterocyte host cells, leading to diarrheal disease. The T3SS is encoded within the chromosomal locus of enterocyte effacement (LEE). The function of some of the LEE-encoded proteins re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01215-12
更新日期:2012-11-01 00:00:00
abstract::Lipoprotein anchoring in bacteria is mediated by the prolipoprotein diacylglyceryl transferase (Lgt), which catalyzes the transfer of a diacylglyceryl moiety to the prospective N-terminal cysteine of the mature lipoprotein. Deletion of the lgt gene in the gram-positive pathogen Listeria monocytogenes (i) impairs intra...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00976-06
更新日期:2007-01-01 00:00:00
abstract::Twelve mutants of Escherichia coli K-12 have been isolated which carry chromosomal mutations that exhibit pleiotropic effects on the expression of F factor tra cistrons. F pilus synthesis, deoxyribonucleic acid transfer, and surface exclusion are all inhibited. Six of the mutants carry sfrA mutations, and six carry sf...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.3.730-737.1979
更新日期:1979-09-01 00:00:00
abstract::From Xanthomonas campestris pv. phaseoli, we have isolated by two independent methods genes involved in peroxide detoxification (ahpC and ahpF), a gene involved in peroxide sensing and transcription regulation (oxyR), and a gene of unknown function (orfX). Amino acid sequence analysis of AhpC, AhpF, and OxyR showed hi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.12.3944-3949.1997
更新日期:1997-06-01 00:00:00
abstract::An alcohol dehydrogenase (ADH) from hyperthermophilic archaeon Thermococcus guaymasensis was purified to homogeneity and was found to be a homotetramer with a subunit size of 40 ± 1 kDa. The gene encoding the enzyme was cloned and sequenced; this gene had 1,095 bp, corresponding to 365 amino acids, and showed high seq...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01433-10
更新日期:2011-06-01 00:00:00
abstract::Bacillus brevis 47 contains two cell wall proteins termed the outer wall protein (OWP) and middle wall protein (MWP), each of which forms hexagonal arrays in the cell wall. A 6-kilobase BglII-BclI fragment of B. brevis 47 DNA cloned into Bacillus subtilis with a derivative of pHW1 as a vector directed the synthesis of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.1.365-373.1986
更新日期:1986-10-01 00:00:00
abstract::Some Escherichia coli strains with impaired cell division form branched cells at high frequencies during certain growth conditions. Here, we show that neither FtsI nor FtsZ activity is required for the development of branches. Buds did not form at specific positions along the cell surface during high-branching conditi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.21.6607-6614.1999
更新日期:1999-11-01 00:00:00
abstract::RecAX53 is a chimeric variant of the Escherichia coli RecA protein (RecAEc) that contains a part of the central domain of Pseudomonas aeruginosa RecA (RecAPa), encompassing a region that differs from RecAEc at 12 amino acid positions. Like RecAPa, this chimera exhibits hyperrecombination activity in E. coli cells, inc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01006-07
更新日期:2008-04-01 00:00:00
abstract::In the filamentous, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120, expression of the nitrate assimilation nirA operon takes place in the absence of ammonium and the presence of nitrate or nitrite. Several positive-action proteins that are required for expression of the nirA operon have been identified...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01668-09
更新日期:2010-06-01 00:00:00
abstract::The R factor NR1 consists of two components: a resistance transfer factor which harbors the tetracycline resistance genes (RTF-TC) and the r-determinants component which harbors the other drug resistance genes. Using partial denaturation mapping it is possible to distinguish the RTF-TC region from the r-determinants r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.123.3.1035-1042.1975
更新日期:1975-09-01 00:00:00
abstract::In aquatic environments, Caulobacter spp. can be found at the boundary between liquid and air known as the neuston. I report an approach to study temporal features of Caulobacter crescentus colonization and pellicle biofilm development at the air-liquid interface and have defined the role of cell surface structures in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00064-19
更新日期:2019-08-22 00:00:00
abstract::A P22 hybrid phage bearing the bacteriophage T4 lysozyme gene (e), as well as T4 sequences upstream from the lysozyme gene, was constructed. Amber mutations were introduced into gene e in the hybrid phage, and the resulting mutant phages were tested for the ability to form plaques on amber suppressor strains. Revertan...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.10.4630-4636.1987
更新日期:1987-10-01 00:00:00
abstract::Using mixed-species cultures, we have undertaken a study of interactions between two common spore-forming soil bacteria, Bacillus subtilis and Streptomyces coelicolor. Our experiments demonstrate that the development of aerial hyphae and spores by S. coelicolor is inhibited by surfactin, a lipopeptide surfactant produ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00162-06
更新日期:2006-07-01 00:00:00
abstract::We studied the following two aspects of the glucose effect on galactose operon expression in Escherichia coli K-12: catabolite repression and inducer exclusion. Using both inducible and constitutive strains and measuring the rate of promoter-proximal enzyme synthesis, we found that the galactose operon did not seem to...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.146.1.149-154.1981
更新日期:1981-04-01 00:00:00
abstract::Sinorhizobium meliloti is a soil bacterium capable of invading and establishing a symbiotic relationship with alfalfa plants. This invasion process requires the synthesis, by S. meliloti, of at least one of the two symbiotically important exopolysaccharides, succinoglycan and EPS II. We have previously shown that the ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.1.325-331.2003
更新日期:2003-01-01 00:00:00
abstract::Bacterial carboxyl-terminal processing proteases (CTPs) are widely conserved and have been linked to important processes including signal transduction, cell wall metabolism, and virulence. However, the features that target proteins for CTP-dependent cleavage are unclear. Studies of the Escherichia coli CTP Prc suggest...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00174-20
更新日期:2020-06-01 00:00:00
abstract::Weiss, Emilio (Naval Medical Research Institute, Bethesda, Md.) and Harry R. Dressler. Increased resistance to chloramphenicol in Rickettsia prowazekii with a note on failure to demonstrate genetic interaction among strains. J. Bacteriol. 83:409-414. 1962.-A further effort to produce genetically labeled Rickettsia pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.2.409-414.1962
更新日期:1962-02-01 00:00:00
abstract::Aspergillus fumigatus, an important opportunistic pathogen which commonly affects neutropenic patients, produces conidia with a bluish-green color. We identified a gene, alb1, which is required for conidial pigmentation. The alb1 gene encodes a putative polyketide synthase, and disruption of alb1 resulted in an albino...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.12.3031-3038.1998
更新日期:1998-06-01 00:00:00