Mutations in an upstream regulatory sequence that increase expression of the bacteriophage T4 lysozyme gene.

abstract：:It previously has been shown that lkyD mutants of Salmonella typhimurium form large blebs of outer membrane over the septal and polar regions of dividing cells. To determine whether the outer membrane blebs are formed over potential sites of division even in the absence of septal ingrowth, lkyD strains were studied un...

journal_title：Journal of bacteriology

authors： Fung JC,MacAlister TJ,Weigand RA,Rothfield LI

更新日期：1980-08-01 00:00:00

abstract：:RsbT is an essential component of the pathway that activates the Bacillus subtilis sigma(B) transcription factor in response to physical stress. rsbT is located within an operon that includes the genes for its principal negative regulator (RsbS) and the stress pathway component that it activates (RsbU), as immediate u...

journal_title：Journal of bacteriology

authors： Zhang S,Reeves A,Woodbury RL,Haldenwang WG

更新日期：2005-12-01 00:00:00

abstract：:We isolated a new recF mutant of Escherichia coli K-12 by insertion of transposon Tn5 into the recF gene. This recF400::Tn5 allele displayed the same phenotypic characteristics as the classic recF143 mutation. By using Mu d(Ap lac) fusions, the induction of nine SOS genes, including recA, umuC, dinA, dinB, dinD, dinF,...

journal_title：Journal of bacteriology

authors： Thoms B,Wackernagel W

更新日期：1987-04-01 00:00:00

abstract：:A noncomposite transposon, designated Tn5541, was isolated from strain Fd33 of the filamentous cyanobacterium Fremyella diplosiphon UTEX 481. Sequence analysis showed that Tn5541 is structurally and genetically very similar to Tn5469, which is also endogenous to F. diplosiphon. Both Tn5469 and Tn5541 encode homologous...

journal_title：Journal of bacteriology

authors： Schaefer MR,Kahn K

更新日期：1998-11-01 00:00:00

abstract：:Dinitrogenase reductase-activating glycohydrolase (DRAG), involved in the regulation of nitrogenase activity in Rhodospirillum rubrum, is associated with chromatophore membranes in cell extracts. We show that DRAG can be specifically released by treatment with MgGDP; other nucleotides studied had no effect. The DRAG a...

journal_title：Journal of bacteriology

authors： Norén A,Nordlund S

更新日期：1997-12-01 00:00:00

abstract：UNLABELLED:TcpP and ToxR coordinately regulate transcription of toxT, the master regulator of numerous virulence factors in Vibrio cholerae. TcpP and ToxR are membrane-localized transcription factors, each with a periplasmic domain containing two cysteines. In ToxR, these cysteines form an intramolecular disulfide bond...

journal_title：Journal of bacteriology

authors： Morgan SJ,French EL,Thomson JJ,Seaborn CP,Shively CA,Krukonis ES

更新日期：2015-11-16 00:00:00

abstract：:DNA polymerase activities in fractionated cell extract of Aeropyrum pernix, a hyperthermophilic crenarchaeote, were investigated. Aphidicolin-sensitive (fraction I) and aphidicolin-resistant (fraction II) activities were detected. The activity in fraction I was more heat stable than that in fraction II. Two different ...

journal_title：Journal of bacteriology

authors： Cann IK,Ishino S,Nomura N,Sako Y,Ishino Y

更新日期：1999-10-01 00:00:00

abstract：:The SasG surface protein of Staphylococcus aureus has been shown to promote the formation of biofilm. SasG comprises an N-terminal A domain and repeated B domains. Here we demonstrate that SasG is involved in the accumulation phase of biofilm, a process that requires a physiological concentration of Zn(2+). The B doma...

journal_title：Journal of bacteriology

authors： Geoghegan JA,Corrigan RM,Gruszka DT,Speziale P,O'Gara JP,Potts JR,Foster TJ

更新日期：2010-11-01 00:00:00

abstract：:During bacteriophage T7 infection, the Escherichia coli RNA polymerase beta' subunit is phosphorylated by the phage-encoded kinase Gp0.7. Here, we used proteolytic degradation and mutational analysis to localize the phosphorylation site to a single amino acid, Thr(1068), in the evolutionarily hypervariable segment of ...

journal_title：Journal of bacteriology

authors： Severinova E,Severinov K

更新日期：2006-05-01 00:00:00

abstract：:This article contains an analysis of a version of the well-known inhibitor-dilution model for the control of initiation of chromosome replication in bacteria. According to this model, an unstable inhibitor interacts with an initiation primer in a hit-and-destroy fashion to prevent successful initiation; both constitue...

journal_title：Journal of bacteriology

authors： Margalit H,Grover NB

更新日期：1987-11-01 00:00:00

abstract：:Shiga toxin (STX), a bacterial toxin produced by Shigella dysenteriae type 1, is a hexamer composed of five receptor-binding B subunits which encircle an alpha-helix at the carboxyl terminus of the enzymatic A polypeptide. Hybrid toxins constructed by fusing the A polypeptide sequences of STX and Shiga-like toxin type...

journal_title：Journal of bacteriology

authors： Jemal C,Haddad JE,Begum D,Jackson MP

更新日期：1995-06-01 00:00:00

abstract：:Outer membrane preparations of Bdellovibrio bacteriovorus grown intraperiplasmically on Escherichia coli containing OmpF were prepared by the Triton X-100 procedure of Schnaitman (J. Bacteriol. 108:545-552, 1971). They contained a protein that migrated to almost the same position as E. coli OmpF in sodium dodecyl sulf...

journal_title：Journal of bacteriology

authors： Rayner JR,Cover WH,Martinez RJ,Rittenberg SC

更新日期：1985-08-01 00:00:00

abstract：:Cyanobacteria are monophyletic organisms that perform oxygenic photosynthesis. While they exhibit great diversity, they have a common set of genes. However, the essentiality of them for viability has hampered the elucidation of their functions. One example of these genes is cyabrB1 (also known as calA in Anabaena sp. ...

journal_title：Journal of bacteriology

authors： Higo A,Nishiyama E,Nakamura K,Hihara Y,Ehira S

更新日期：2019-08-08 00:00:00

abstract：:IS630 is a 1.15-kilobase sequence in Shigella sonnei that, unlike many mobile elements, seems not to mediate cointegration between different replicons. To assess its transposition, we constructed composite elements containing inverted copies of IS630 flanking a drug resistance gene. We found that these composite eleme...

journal_title：Journal of bacteriology

authors： Tenzen T,Matsutani S,Ohtsubo E

更新日期：1990-07-01 00:00:00

abstract：:The determinant of resistance to fosfomycin of the Serratia marcescens plasmid pOU900 was transposed into the plasmid ColE1 and into the plasmid RP4 in the absence of the RecA function of the host. In each case, the acquisition of fosfomycin resistance was correlated with the presence of a discrete piece of DNA, unifo...

journal_title：Journal of bacteriology

authors： García-Lobo JM,Ortiz JM

更新日期：1982-07-01 00:00:00

abstract：:Escherichia coli W3110 contains four types of IS1 elements in the chromosome. Using an insertion element entrapping system, we collected 116 IS1 plasmid insertion mutants, which resulted from a minimum of 26 independent IS1 insertion events. All of them had insertions of IS1 of the IS1A (IS1E and IS1G) type. Inspectio...

journal_title：Journal of bacteriology

authors： Chen JH,Hsu WB,Hwang JL

更新日期：1998-10-01 00:00:00

abstract：:Catabolic mannopine (MOP) cyclase encoded by certain Agrobacterium Ti and Ri plasmids lactonizes MOP to agropine (AGR). The enzyme, purified to homogeneity from a recombinant clone, has a molecular mass of 45 kDa as measured by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and size exclusion chromatography...

journal_title：Journal of bacteriology

authors： Hong SB,Farrand SK

更新日期：1996-04-01 00:00:00

abstract：:The carbon monoxide (CO) dehydrogenase activity band from Clostridium pasteurianum was shown to contain nickel by in situ activity staining of polyacrylamide gels. However, the majority of the nickel in cell extracts was found to electrophorese independently of CO dehydrogenase. Comparative analysis with Clostridium t...

journal_title：Journal of bacteriology

authors： Drake HL

更新日期：1982-02-01 00:00:00

abstract：:Outer membranes, free of cytoplasmic or thylakoid membranes and peptidoglycan components, were obtained from Synechocystis sp. strain PCC6714. Electron microscope studies revealed double-track outer membrane vesicles with a smooth-appearing exoplasmic surface, an exoplasmic fracture face covered by closely packed part...

journal_title：Journal of bacteriology

authors： Jürgens UJ,Weckesser J

更新日期：1985-10-01 00:00:00

abstract：:The CO dehydrogenase structural genes (cox) and orf4 are clustered in the transcriptional order coxM--> coxS--> coxL--> orf4 on the 128-kb megaplasmid pHCG3 of the carboxidotroph Oligotropha carboxidovorans OM5. Sequence analysis suggested association of molybdopterin cytosine dinucleotide and flavin adenine dinucleot...

journal_title：Journal of bacteriology

authors： Schübel U,Kraut M,Mörsdorf G,Meyer O

更新日期：1995-04-01 00:00:00

abstract：:In bacteria, a subset of membrane proteins insert into the membrane via the Sec apparatus with the assistance of the widely conserved essential membrane protein insertase YidC. After threading into the SecYEG translocon, transmembrane segments of nascent proteins are thought to exit the translocon via a lateral gate i...

journal_title：Journal of bacteriology

authors： Li Z,Boyd D,Reindl M,Goldberg MB

更新日期：2014-01-01 00:00:00

abstract：:The biosynthesis of the thaxtomin cyclic dipeptide phytotoxins proceeds nonribosomally via the thiotemplate mechanism. Acyladenylation, thioesterification, N-methylation, and cyclization of two amino acid substrates are catalyzed by the txtAB-encoded thaxtomin synthetase. Nucleotide sequence analysis of the region 3' ...

journal_title：Journal of bacteriology

authors： Healy FG,Krasnoff SB,Wach M,Gibson DM,Loria R

更新日期：2002-04-01 00:00:00

abstract：:We previously described a bacteriophage of the Lyme disease agent Borrelia burgdorferi designated phiBB-1. This phage packages the host complement of the 32-kb circular plasmids (cp32s), a group of homologous molecules found throughout the genus Borrelia. To demonstrate the ability of phiBB-1 to package and transduce ...

journal_title：Journal of bacteriology

authors： Eggers CH,Kimmel BJ,Bono JL,Elias AF,Rosa P,Samuels DS

更新日期：2001-08-01 00:00:00

abstract：:The synthesis of cytosine deaminase in Salmonella typhimurium is repressed by pyrimidines. This repression is mediated by both a uridine and a cytidine compound, indicating a distinct difference in the regulation of synthesis of cytosine deaminase from the regulation of the de novo pyrimidine pathway enzymes. A salvag...

journal_title：Journal of bacteriology

authors： West TP,O'Donovan GA

更新日期：1982-03-01 00:00:00

abstract：:Hauge, Jens G. (National Institute for Public Health, Oslo, Norway). Glucose dehydrogenation in bacteria: a comparative study. J. Bacteriol. 82:609-614. 1961.-Extracts of a series of bacteria reported to convert glucose to gluconic acid via particulate enzyme systems were fractionated by differential centrifugation in...

journal_title：Journal of bacteriology

authors： HAUGE JG

更新日期：1961-10-01 00:00:00

abstract：:Clostridium perfringens is an anaerobic Gram-positive pathogen that causes many human and animal diseases, including food poisoning and gas gangrene. C. perfringens lacks flagella but possesses type IV pili (TFP). We have previously shown that C. perfringens can glide across an agar surface in long filaments composed ...

journal_title：Journal of bacteriology

authors： Liu H,Bouillaut L,Sonenshein AL,Melville SB

更新日期：2013-02-01 00:00:00

abstract：:In a previous study, we showed that in bacteroids, transcription of the inducible nod genes does not occur and expression of nodD is decreased by 65% (H. R. M. Schlaman, B. Horvath, E. Vijgenboom, R.J.H. Okker, and B. J. J. Lugtenberg, J. Bacteriol. 173:4277-4287, 1991). In the present study, we show, using gel retard...

journal_title：Journal of bacteriology

authors： Schlaman HR,Lugtenberg BJ,Okker RJ

更新日期：1992-10-01 00:00:00

abstract：:The effect of various salts on the autolysis of cell wall of a ribitol teichoic acid-deficient mutant of Staphylococcus aureus H (strain 52A5 carrying tar-1) was compared with the parent strain. In the presence of high concentrations of certain salts such as 1.0 m NaCl, the mutant undergoes autolysis with the release ...

journal_title：Journal of bacteriology

authors： Gilpin RW,Chatterjee AN,Young FE

更新日期：1972-07-01 00:00:00

abstract：:Sequence flanking the bfpA locus on the enteroadherent factor plasmid of the enteropathogenic Escherichia coli (EPEC) strain B171-8 (O111:NM) was obtained to identify genes that might be required for bundle-forming pilus (BFP) biosynthesis. Deletion experiments led to the identification of a contiguous cluster of at l...

journal_title：Journal of bacteriology

authors： Sohel I,Puente JL,Ramer SW,Bieber D,Wu CY,Schoolnik GK

更新日期：1996-05-01 00:00:00

abstract：:Campylobacter jejuni 81-176 lipooligosaccharide (LOS) is composed of two covalently linked domains: lipid A, a hydrophobic anchor, and a nonrepeating core oligosaccharide, consisting of an inner and outer core region. We report the isolation and characterization of the deepest rough C. jejuni 81-176 mutant by insertio...

journal_title：Journal of bacteriology

authors： Kanipes MI,Papp-Szabo E,Guerry P,Monteiro MA

更新日期：2006-05-01 00:00:00