Dinitrogenase reductase-activating glycohydrolase can be released from chromatophores of Rhodospirillum rubrum by treatment with MgGDP.

Abstract:

:Dinitrogenase reductase-activating glycohydrolase (DRAG), involved in the regulation of nitrogenase activity in Rhodospirillum rubrum, is associated with chromatophore membranes in cell extracts. We show that DRAG can be specifically released by treatment with MgGDP; other nucleotides studied had no effect. The DRAG activity released corresponds to the release of DRAG protein.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Norén A,Nordlund S

doi

10.1128/jb.179.24.7872-7874.1997

subject

Has Abstract

pub_date

1997-12-01 00:00:00

pages

7872-4

issue

24

eissn

0021-9193

issn

1098-5530

journal_volume

179

pub_type

杂志文章
  • Tn5401, a new class II transposable element from Bacillus thuringiensis.

    abstract::A new class II (Tn3-like) transposable element, designated Tn5401, was recovered from a sporulation-deficient variant of Bacillus thuringiensis subsp. morrisoni EG2158 following its insertion into a recombinant plasmid. Sequence analysis of the insert revealed a 4,837-bp transposon with two large open reading frames, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.10.2835-2845.1994

    authors: Baum JA

    更新日期:1994-05-01 00:00:00

  • An Eight-Residue Deletion in Escherichia coli FabG Causes Temperature-Sensitive Growth and Lipid Synthesis Plus Resistance to the Calmodulin Inhibitor Trifluoperazine.

    abstract::FabG performs the NADPH-dependent reduction of β-keto acyl-acyl carrier protein substrates in the elongation cycle of fatty acid synthesis. We report the characterization of a temperature-sensitive mutation (fabGΔ8) in Escherichia colifabG that results from an in-frame 8-amino-acid residue deletion in the α6/α7 subdom...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00074-17

    authors: Srinivas S,Cronan JE

    更新日期:2017-04-25 00:00:00

  • Transcriptional regulation of the heterocyst patterning gene patA from Anabaena sp. strain PCC 7120.

    abstract::The filamentous cyanobacterium Anabaena sp. strain PCC 7120 forms a periodic pattern of nitrogen-fixing heterocysts when grown in the absence of combined nitrogen. PatA is necessary for proper patterning of heterocysts along filaments. In this study, apparent transcriptional start points (tsps) were identified at nucl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00577-10

    authors: Young-Robbins SS,Risser DD,Moran JR,Haselkorn R,Callahan SM

    更新日期:2010-09-01 00:00:00

  • Polyethylene glycol-facilitated transformation of Bacteroides fragilis with plasmid DNA.

    abstract::A method for the transformation of Bacteroides fragilis with plasmid DNA was developed by using the clindamycin resistance plasmid pBFTM10 as the source of transforming DNA. The method was technically simple to perform and resulted in an average of 4.2 X 10(3) transformants per microgram of pBFTM10 added. A method for...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.1.466-469.1985

    authors: Smith CJ

    更新日期:1985-10-01 00:00:00

  • Antigens of Bordetella pertussis. II. Purification of heat-labile toxin.

    abstract::Banerjea, A. (Rocky Mountain Laboratory, Hamilton, Mont.) and J. Munoz. Antigens of Bordetella pertussis. II, Purification of heat-labile toxin. J. Bacteriol. 84:269-274. 1962.-A mild method of separating heat-labile toxin of Bordetella pertussis from other cellular components is described; it consists of absorbing to...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.84.2.269-274.1962

    authors: BANERJEA A,MUNOZ J

    更新日期:1962-08-01 00:00:00

  • Chemical and biochemical changes in subcellular fractions of guinea pig liver during infection with Coxiella burneti.

    abstract::Livers of uninfected guinea pigs and of guinea pigs infected with Coxiella burneti were fractionated into smooth endoplasmic reticulum, rough endoplasmic reticulum (RER), pellet, and cell sap fractions. The ribonucleic acid (RNA) and protein of each fraction were determined, and the phosphorylase, glucose-6-phosphatas...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.102.2.334-340.1970

    authors: Paretsky D,Stueckemann J

    更新日期:1970-05-01 00:00:00

  • Evidence for multiple OmpR-binding sites in the upstream activation sequence of the ompC promoter in Escherichia coli: a single OmpR-binding site is capable of activating the promoter.

    abstract::We present experimental evidence for the existence of multiple activator-binding sites in the upstream sequence of the ompC promoter, the expression of which is activated by the positive regulator OmpR in response to the osmolarity of the medium. We also found that a single OmpR-binding site can activate the ompC prom...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.1.501-503.1990

    authors: Maeda S,Mizuno T

    更新日期:1990-01-01 00:00:00

  • ANTIMICROBIAL ACTION OF NITROGEN-CONTAINING STEROIDS.

    abstract::Smith, Rodney F. (University of Maryland, Baltimore), Donald E. Shay, and Norman J. Doorenbos. Antimicrobial action of nitrogen-containing steroids. J. Bacteriol. 85:1295-1299. 1963.-A new group of 16 synthetic nitrogen-containing steroids have been tested against a variety of microorganisms for antimicrobial properti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.85.6.1295-1299.1963

    authors: SMITH RF,SHAY DE,DOORENBOS NJ

    更新日期:1963-06-01 00:00:00

  • A-factor and phosphate depletion signals are transmitted to the grixazone biosynthesis genes via the pathway-specific transcriptional activator GriR.

    abstract::Grixazone (GX), which is a diffusible yellow pigment containing a phenoxazinone chromophore, is one of the secondary metabolites under the control of A-factor (2-isocapryloyl-3R-hydroxymethyl-gamma-butyrolactone) in Streptomyces griseus. GX production is also induced by phosphate starvation. The whole biosynthesis gen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00055-07

    authors: Higashi T,Iwasaki Y,Ohnishi Y,Horinouchi S

    更新日期:2007-05-01 00:00:00

  • Crystal structure of the cystic fibrosis transmembrane conductance regulator inhibitory factor Cif reveals novel active-site features of an epoxide hydrolase virulence factor.

    abstract::Cystic fibrosis transmembrane conductance regulator (CFTR) inhibitory factor (Cif) is a virulence factor secreted by Pseudomonas aeruginosa that reduces the quantity of CFTR in the apical membrane of human airway epithelial cells. Initial sequence analysis suggested that Cif is an epoxide hydrolase (EH), but its seque...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01348-09

    authors: Bahl CD,Morisseau C,Bomberger JM,Stanton BA,Hammock BD,O'Toole GA,Madden DR

    更新日期:2010-04-01 00:00:00

  • Bacteriophage SPP1 Chu is an alkaline exonuclease in the SynExo family of viral two-component recombinases.

    abstract::Many DNA viruses concatemerize their genomes as a prerequisite to packaging into capsids. Concatemerization arises from either replication or homologous recombination. Replication is already the target of many antiviral drugs, and viral recombinases are an attractive target for drug design, particularly for combinatio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.8.2465-2474.2003

    authors: Vellani TS,Myers RS

    更新日期:2003-04-01 00:00:00

  • The thiol:disulfide oxidoreductase DsbB mediates the oxidizing effects of the toxic metalloid tellurite (TeO32-) on the plasma membrane redox system of the facultative phototroph Rhodobacter capsulatus.

    abstract::The highly toxic oxyanion tellurite (TeO3(2-)) is a well known pro-oxidant in mammalian and bacterial cells. This work examines the effects of tellurite on the redox state of the electron transport chain of the facultative phototroph Rhodobacter capsulatus, in relation to the role of the thiol:disulfide oxidoreductase...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01080-06

    authors: Borsetti F,Francia F,Turner RJ,Zannoni D

    更新日期:2007-02-01 00:00:00

  • Recombination in the ompA gene but not the omcB gene of Chlamydia contributes to serovar-specific differences in tissue tropism, immune surveillance, and persistence of the organism.

    abstract::Sequences of the major outer membrane protein (MOMP) gene (ompA) and the outer membrane complex B protein gene (omcB) from Chlamydia trachomatis, Chlamydia pneumoniae, and Chlamydia psittaci were analyzed for evidence of intragenic recombination and for linkage equilibrium. The Sawyer runs test, compatibility matrices...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.20.5997-6008.2001

    authors: Millman KL,Tavaré S,Dean D

    更新日期:2001-10-01 00:00:00

  • Glucose metabolism in gcr mutants of Saccharomyces cerevisiae.

    abstract::A gcr2 null mutant of Saccharomyces cerevisiae grows well on glucose in spite of its lower level of glycolytic enzymes between triose phosphates and pyruvate. A quantitative analysis shows that these levels are adequate to the flux but glycerate phosphates are elevated. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.15.4719-4723.1999

    authors: Uemura H,Fraenkel DG

    更新日期:1999-08-01 00:00:00

  • nfi, the gene for endonuclease V in Escherichia coli K-12.

    abstract::Endonuclease V is specific for single-stranded DNA or for duplex DNA that contains uracil or that is damaged by a variety of agents (B. Demple and S. Linn, J. Biol. Chem. 257:2848-2855, 1982). Thus, it may be a versatile DNA repair enzyme. The protein was purified to apparent homogeneity, and from its N-terminal seque...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.2.310-316.1997

    authors: Guo G,Ding Y,Weiss B

    更新日期:1997-01-01 00:00:00

  • Sporulation in D-glucosamine auxotrophs of Saccharomyces cerevisiae: meiosis with defective ascospore wall formation.

    abstract::Mutants that require exogenous D-glucosamine for growth were isolated from Saccharomyces cerevisiae X2180-1A after ethyl methane sulfonate mutagenesis. Class A auxotrophs fail to grow on yeast extract-peptone-dextrose and minimal media, whereas class B auxotrophs grow on minimal medium and readily revert to grow on ye...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.124.3.1545-1557.1975

    authors: Whelan WL,Ballou CE

    更新日期:1975-12-01 00:00:00

  • Activation of inactive nitrogenase by acid-treated component I.

    abstract::When Azotobacter vinelandii was derepressed for nitrogenase synthesis in a N-free medium containing tungstate instead of molybdate, an inactive component I was synthesized. Although this inactive component I could be activated in vivo upon addition of molybdate to the medium, it could not be activated in vitro when mo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.120.2.697-701.1974

    authors: Nagatani HH,Shah VK,Brill WJ

    更新日期:1974-11-01 00:00:00

  • Protein mobility in the cytoplasm of Escherichia coli.

    abstract::The rate of protein diffusion in bacterial cytoplasm may constrain a variety of cellular functions and limit the rates of many biochemical reactions in vivo. In this paper, we report noninvasive measurements of the apparent diffusion coefficient of green fluorescent protein (GFP) in the cytoplasm of Escherichia coli. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.1.197-203.1999

    authors: Elowitz MB,Surette MG,Wolf PE,Stock JB,Leibler S

    更新日期:1999-01-01 00:00:00

  • Chloramphenicol-sensitive and-insensitive phases of the lethal action of streptomycin.

    abstract::Hurwitz, Charles (Veterans Administration Hospital, Albany, N.Y.) and Carmen L. Rosano. Chloramphenicol-sensitive and -insensitive phases of the lethal action of streptomycin. J. Bacteriol. 83:1202-1209. 1962.-If chloramphenicol is added to sensitive Escherichia coli cells at the same time as streptomycin, the lethal ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.6.1202-1209.1962

    authors: HURWITZ C,ROSANO CL

    更新日期:1962-06-01 00:00:00

  • Nuclear and cell division in Bacillus subtilis: dormant nucleoids in stationary-phase cells and their activation.

    abstract::The morphology of nucleoids and mesosomes of Bacillus subtilis in stationary-and lag-phase cultures was studied by making three-dimensional cell reconstructions in plastic of electron micrographs of serial sections. In cells from stationary cultures, the dormant nucleoids are frequently, but not always, spherical and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.121.3.1189-1199.1975

    authors: Van Iterson W,Michels PA,Vyth-Dreese F,Aten JA

    更新日期:1975-03-01 00:00:00

  • Ultrastructural properties of the extra membranes of Escherichia coli O111a as revealed by freeze-fracturing and negative-staining techniques.

    abstract::Escherichia coli O111a is a thermosensitive strain which, when grown at 40 C, accumulates large quantities of intracellular membranes. The ultrastructure of these membranes in cells which have been chemically fixed, embedded, and examined as thin sections has been compared with that of membranes in cells negatively st...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.1.433-444.1973

    authors: Weigand RA,Holt SC,Shively JM,Decker GL,Greenawalt JW

    更新日期:1973-01-01 00:00:00

  • Nucleotide and derived amino acid sequences of the major porin of Comamonas acidovorans and comparison of porin primary structures.

    abstract::The DNA sequence of the gene which codes for the major outer membrane porin (Omp32) of Comamonas acidovorans has been determined. The structural gene encodes a precursor consisting of 351 amino acid residues with a signal peptide of 19 amino acid residues. Comparisons with amino acid sequences of outer membrane protei...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.7.2196-2205.1991

    authors: Gerbl-Rieger S,Peters J,Kellermann J,Lottspeich F,Baumeister W

    更新日期:1991-04-01 00:00:00

  • Draft genome sequence of Gordonia neofelifaecis NRRL B-59395, a cholesterol-degrading actinomycete.

    abstract::We report a draft sequence of the genome of Gordonia neofelifaecis NRRL B-59395, a cholesterol-degrading actinomycete isolated from fresh feces of a clouded leopard (Neofelis nebulosa). As predicted, the reported genome contains several gene clusters for cholesterol degradation. This is the second available genome seq...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05531-11

    authors: Ge F,Li W,Chen G,Liu Y,Zhang G,Yong B,Wang Q,Wang N,Huang Z,Li W,Wang J,Wu C,Xie Q,Liu G

    更新日期:2011-09-01 00:00:00

  • Factors affecting cellulolysis by Ruminococcus albus.

    abstract::The factors influencing the digestion of pebble-milled cellulose by enzymes were studied by using several strains of Ruminococcus albus including a mutant characterized by a more eccentric location of its colony in the clearing produced by digestion of the cellulose in the thin layer lining the wall of a culture tube....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.114.2.729-737.1973

    authors: Smith WR,Yu I,Hungate RE

    更新日期:1973-05-01 00:00:00

  • Effect of cell cycle position on thermotolerance in Saccharomyces cerevisiae.

    abstract::We showed that the heat killing curve for exponentially growing Saccharomyces cerevisiae was biphasic. This suggests two populations of cells with different thermal killing characteristics. When exponentially growing cells separated into cell cycle-specific fractions via centrifugal elutriation were heat shocked, the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.2.779-784.1987

    authors: Plesset J,Ludwig JR,Cox BS,McLaughlin CS

    更新日期:1987-02-01 00:00:00

  • Synthesis of lipopolysaccharide O side chains by Pseudomonas aeruginosa PAO1 requires the enzyme phosphomannomutase.

    abstract::We have cloned a lipopolysaccharide (LPS) biosynthetic gene from Pseudomonas aeruginosa PAO1 that complements the defect in the production and incorporation of LPS O side chains in the LPS-rough strain AK1012. This gene was characterized by pulsed-field gel electrophoresis, deletion and restriction mapping of the clon...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.6.1605-1611.1993

    authors: Goldberg JB,Hatano K,Pier GB

    更新日期:1993-03-01 00:00:00

  • Control of the Staphylococcus aureus toxic shock tst promoter by the global regulator SarA.

    abstract::The Staphylococcus aureus SarA global regulator controls the expression of numerous virulence genes, often in conjunction with the agr quorum-sensing system and its effector RNA, RNAIII. In the present study, we have examined the role of both SarA and RNAIII on the regulation of the promoter of tst, encoding staphyloc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00146-10

    authors: Andrey DO,Renzoni A,Monod A,Lew DP,Cheung AL,Kelley WL

    更新日期:2010-11-01 00:00:00

  • Biosynthesis of the outer membrane receptor for vitamin B12, E colicins, and bacteriophage BF23 by Escherichia coli: kinetics of phenotypic expression after the introduction of bfe+ and bfe alleles.

    abstract::The bfe locus codes for the cell surface receptor for vitamin B12, the E colicins, and bacteriophage BF23 in the Escherichia coli outer membrane. When the bfe+ allele, which is closely linked to the argH locus, was introduced into an argH bfe recipient by conjugation, arg+ recombinant cells rapidly and simultaneously ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.129.1.265-275.1977

    authors: Bassford PJ Jr,Kadner RJ,Schnaitman CA

    更新日期:1977-01-01 00:00:00

  • Tryptophan biosynthesis in Salmonella typhimurium: location in trpB of a genetic difference between strains LT2 and LT7.

    abstract::Salmonella typhimurium prototrophs carrying a trpR mutation synthesize tryptophan biosynthetic enzymes constitutively. When feedback inhibition of anthranilate synthetase but not 5'-phosphoribosylpyrophosphate phosphoribosyltransferase activity was by-passed by growing cells on media supplemented with anthranilic acid...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.123.3.878-887.1975

    authors: Stuttard C

    更新日期:1975-09-01 00:00:00

  • Global mutational analysis of NtrC-like activators in Myxococcus xanthus: identifying activator mutants defective for motility and fruiting body development.

    abstract::The multicellular developmental cycle of Myxococcus xanthus requires large-scale changes in gene transcription, and recent findings indicate that NtrC-like activators play a prominent role in regulating these changes. In this study, we made insertions in 28 uncharacterized ntrC-like activator (nla) genes and found tha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.20.6083-6094.2003

    authors: Caberoy NB,Welch RD,Jakobsen JS,Slater SC,Garza AG

    更新日期:2003-10-01 00:00:00