Effect of cell cycle position on thermotolerance in Saccharomyces cerevisiae.

abstract：:The Escherichia coli cell division protein FtsQ is a central component of the divisome. FtsQ is a bitopic membrane protein with a large C-terminal periplasmic domain. In this work we investigated the role of the transmembrane segment (TMS) that anchors FtsQ in the cytoplasmic membrane. A set of TMS mutants was made an...

journal_title：Journal of bacteriology

authors： Scheffers DJ,Robichon C,Haan GJ,den Blaauwen T,Koningstein G,van Bloois E,Beckwith J,Luirink J

更新日期：2007-10-01 00:00:00

abstract：:Sixteen species of enterobacteria have been screened for mutagenic DNA repair activity. In Escherichia coli, mutagenic DNA repair is encoded by the umuDC operon. Synthesis of UmuD and UmuC proteins is induced as part of the SOS response to DNA damage, and after induction, the UmuD protein undergoes an autocatalytic cl...

journal_title：Journal of bacteriology

authors： Sedgwick SG,Ho C,Woodgate R

更新日期：1991-09-01 00:00:00

abstract：:The mechanisms by which Legionella pneumophila, a facultative intracellular parasite and the agent of Legionnaires' disease, acquires iron are largely unexplained. Several earlier studies indicated that L. pneumophila does not elaborate siderophores. However, we now present evidence that supernatants from L. pneumophi...

journal_title：Journal of bacteriology

authors： Liles MR,Scheel TA,Cianciotto NP

更新日期：2000-02-01 00:00:00

abstract：:Previously, the effects of three point mutations (at amino acid positions 31, 84, and 194) in the gene coding for a sulfonamide-resistant dihydropteroate synthase of Neisseria meningitidis were analyzed by site-directed mutagenesis. Changes at positions 31 and 194 abolished the phenotypic expression of sulfonamide res...

journal_title：Journal of bacteriology

authors： Fermér C,Swedberg G

更新日期：1997-02-01 00:00:00

abstract：:Promoters were cloned from the chromosomal DNA of Lactococcus lactis subsp. lactis NP4510 by using promoter-probe vector pGKV210. N-Methyl-N'-nitro-N-nitrosoguanidine-induced mutagenesis of L. lactis-(pBV413), with low-level expression of the cat-86 gene, resulted in enhanced expression. Subcloning and sequencing of t...

journal_title：Journal of bacteriology

authors： Bojovic B,Djordjevic G,Banina A,Topisirovic L

更新日期：1994-11-01 00:00:00

abstract：:The outer membrane of the thermophilic bacterium Thermus thermophilus was isolated using sucrose step gradient centrifugation. Its detergent extracts contained an ion-permeable channel with an extremely high single-channel conductance of 20 nS in 1 M KCl. The channel protein was purified by preparative sodium dodecyl ...

journal_title：Journal of bacteriology

authors： Maier E,Polleichtner G,Boeck B,Schinzel R,Benz R

更新日期：2001-01-01 00:00:00

abstract：:Iron-sulfur flavoproteins (ISF) constitute a widespread family of redox-active proteins in anaerobic prokaryotes. Based on sequence homologies, their overall structure is expected to be similar to that of flavodoxins, but in addition to a flavin mononucleotide cofactor they also contain a cubane-type [4Fe:4S] cluster....

journal_title：Journal of bacteriology

authors： Andrade SL,Cruz F,Drennan CL,Ramakrishnan V,Rees DC,Ferry JG,Einsle O

更新日期：2005-06-01 00:00:00

abstract：:Individually, the ribosomal proteins L1, L23, L36, and S6 are not essential for cell proliferation of Bacillus subtilis, but the absence of any one of these ribosomal proteins causes a defect in the formation of the 70S ribosomes and a reduced growth rate. In mutant strains individually lacking these ribosomal protein...

journal_title：Journal of bacteriology

authors： Akanuma G,Yamazaki K,Yagishi Y,Iizuka Y,Ishizuka M,Kawamura F,Kato-Yamada Y

更新日期：2018-08-24 00:00:00

abstract：:The M-band technique was used to assess the number of attachment points of DNA to the cell membrane of Streptococcus faecalis grown at three different rates. Cells were X irradiated in liquid nitrogen and then analyzed simultaneously for the introduction of double-strand breaks into the chromosome and the degree of re...

journal_title：Journal of bacteriology

authors： Parks LC,Rigney D,Daneo-Moore L,Higgins ML

更新日期：1982-10-01 00:00:00

abstract：:Escherichia coli possesses three distinct formate dehydrogenase enzymes encoded by the fdnGHI, fdhF, and fdoGHI operons. To examine how two of the formate dehyrogenase operons (fdnGHI and fdhF) are expressed anaerobically in the presence of low, intermediate, and high levels of nitrate, nitrite, and formate, chemostat...

journal_title：Journal of bacteriology

authors： Wang H,Gunsalus RP

更新日期：2003-09-01 00:00:00

abstract：:An assay was developed to determine the amount of cyanophycin granules in blue-green algae. The amount of this polypeptide in cells of Anabaena cylindrica was measured as a function of culture age and was compared with changes in other proteinaceous cellular components. The data presented support the notion that the c...

journal_title：Journal of bacteriology

authors： Simon RD

更新日期：1973-06-01 00:00:00

abstract：:The first enzymes of the histidine (hut) and proline degradative pathways, histidase and proline oxidase, could not be induced in Bacillus subtilis cells growing in glucose minimal medium containing a mixture of 16 amino acids. Addition of the 16-amino-acid mixture to induced wild-type cells growing in citrate minimal...

journal_title：Journal of bacteriology

authors： Atkinson MR,Wray LV Jr,Fisher SH

更新日期：1990-09-01 00:00:00

abstract：:The elongation factor Tu, encoded by tuf genes, is a GTP binding protein that plays a central role in protein synthesis. One to three tuf genes per genome are present, depending on the bacterial species. Most low-G+C-content gram-positive bacteria carry only one tuf gene. We have designed degenerate PCR primers derive...

journal_title：Journal of bacteriology

authors： Ke D,Boissinot M,Huletsky A,Picard FJ,Frenette J,Ouellette M,Roy PH,Bergeron MG

更新日期：2000-12-01 00:00:00

abstract：:Cell-free extracts with high nitrogenase activity were prepared by sonic oscillation and French press treatment from the blue-gree alga Anabaena cylindrica. Extracts were prepared from cells grown on a 95% N(2)-5% CO(2) gas mixture followed by a period of nitrogen starvation under an atmosphere of 95% argon-5% CO(2). ...

journal_title：Journal of bacteriology

authors： Smith RV,Evans MC

更新日期：1971-03-01 00:00:00

abstract：:Thin pili of the closely related IncI1 plasmids ColIb-P9 and R64 are required only for liquid mating and belong to the type IV family of pili. They were sedimented by ultracentrifugation from culture medium in which Escherichia coli cells harboring ColIb-P9- or R64-derived plasmids had been grown, and then the pili we...

journal_title：Journal of bacteriology

authors： Yoshida T,Furuya N,Ishikura M,Isobe T,Haino-Fukushima K,Ogawa T,Komano T

更新日期：1998-06-01 00:00:00

abstract：:The mycoplasma Acholeplasma laidlawii was shown to have mechanisms for both host cell and ultraviolet (UV) reactivation of UV-irradiated mycoplasmaviruses. Host cell reactivation was examined by comparing the survival abilities of UV-irradiated double-stranded deoxyribonucleic acid mycoplasmavirus plated on both untre...

journal_title：Journal of bacteriology

authors： Das J,Nowak JA,Maniloff J

更新日期：1977-03-01 00:00:00

abstract：:Using a system of chromatography through columns of DEAE-Bio-Gel, HTP-Bio-Gel, and CM-Bio-Gel, we isolated and characterized six different (1 leads to 3)-beta-glucanases from cell wall autolysates and cell extracts of Saccharomyces cerevisiae haploid strain 2180B. These enzymes were designated glucanases I, II, IIIA, ...

journal_title：Journal of bacteriology

authors： Hien NH,Fleet GH

更新日期：1983-12-01 00:00:00

abstract：:IcsA is an outer membrane protein in the autotransporter family that is required for Shigella flexneri pathogenesis. Following its secretion through the Sec translocon, IcsA is incorporated into the outer membrane in a process that depends on YaeT, a component of an outer membrane beta-barrel insertion machinery. We i...

journal_title：Journal of bacteriology

authors： Wagner JK,Heindl JE,Gray AN,Jain S,Goldberg MB

更新日期：2009-02-01 00:00:00

abstract：:Eight mutants of Bacillus subtilis deficient in xylan utilization were isolated and characterized genetically and biochemically. Each mutant was obtained independently after nitrosoguanidine mutagenesis. All of the analyzed mutations were shown to be linked. Reciprocal transformation crosses revealed the existence of ...

journal_title：Journal of bacteriology

authors： Roncero MI

更新日期：1983-10-01 00:00:00

abstract：:Toxin and antitoxin (TA) gene pairs are addiction systems that are present in many microbial genomes. Sinorhizobium meliloti is an N2-fixing bacterial symbiont of alfalfa and other leguminous plants, and its genome consists of three large replicons, a circular chromosome (3.7 Mb) and the megaplasmids pSymA (1.4 Mb) an...

journal_title：Journal of bacteriology

authors： Milunovic B,diCenzo GC,Morton RA,Finan TM

更新日期：2014-02-01 00:00:00

abstract：:Nitrogenase activity in Rhodospirillum rubrum and in some other photosynthetic bacteria is regulated in part by the availability of light. This regulation is through a posttranslational modification system that is itself regulated by P(II) homologs in the cell. P(II) is one of the most broadly distributed regulatory p...

journal_title：Journal of bacteriology

authors： Zhang Y,Pohlmann EL,Roberts GP

更新日期：2009-09-01 00:00:00

abstract：:The primary sequence of the small cysteine-rich protein (EnvA) of Chlamydia psittaci 6BC has been shown to possess a potential lipid modification/signal peptidase II-processing site, and the mature protein was labeled by a [3H]palmitic acid precursor. We further characterized the mature EnvA, showing that it lacks the...

journal_title：Journal of bacteriology

authors： Everett KD,Desiderio DM,Hatch TP

更新日期：1994-10-01 00:00:00

abstract：:From citrate-utilizing (Cit+) Escherichia coli strain C53 of bovine origin, strains C53A and C53B were obtained. Upon mating with recA+ but not with recA mutant recipients of K-12, C53A produced chromosomal recombinants at quite high frequencies, leading to the following conclusions: (i) C53A is an Hfr strain; (ii) th...

journal_title：Journal of bacteriology

authors： Ishiguro N,Oka C,Asagi M,Sato G,Yoshikawa M

更新日期：1980-08-01 00:00:00

abstract：:In many microorganisms the first step for alkane degradation is the terminal oxidation of the molecule by an alkane hydroxylase. We report the characterization of a gene coding for an alkane hydroxylase in a Burkholderia cepacia strain isolated from an oil-contaminated site. The protein encoded showed similarity to ot...

journal_title：Journal of bacteriology

authors： Marín MM,Smits TH,van Beilen JB,Rojo F

更新日期：2001-07-01 00:00:00

abstract：:The actinomycin synthetases ACMS I, II, and III catalyze the assembly of the acyl peptide lactone precursor of actinomycin by a nonribosomal mechanism. We have cloned the genes of ACMS I (acmA) and ACMS II (acmB) by hybridization screening of a cosmid library of Streptomyces chrysomallus DNA with synthetic oligonucleo...

journal_title：Journal of bacteriology

authors： Schauwecker F,Pfennig F,Schröder W,Keller U

更新日期：1998-05-01 00:00:00

abstract：:Genetic transformation of auxotrophs of the extreme thermophile Thermus thermophilus HB27 to prototrophy was obtained at high frequencies of 10(-2) to 10(-1) when proliferating cell populations were exposed to chromosomal DNA from a nutritionally independent wild-type strain. The transformation frequency was proportio...

journal_title：Journal of bacteriology

authors： Koyama Y,Hoshino T,Tomizuka N,Furukawa K

更新日期：1986-04-01 00:00:00

abstract：:In this communication we present evidence that indicates that 1,2-propanediol (1,2-PDL) is a positive effector of the transcription of the cobalamin (vitamin B12) biosynthetic (cob) operon and of the 1,2-PDL utilization (pdu) genes. The stimulatory effects of 1,2-PDL were demonstrated using Mu d-lac transcriptional fu...

journal_title：Journal of bacteriology

authors： Rondon MR,Escalante-Semerena JC

更新日期：1992-04-01 00:00:00

abstract：:Discadenine,3-(3-amino-3-carboxypropyl)-N6-delta 2-isopentenyladenine, which inhibits spore germination, was previously found in Dictyostelium discoideum. Studies on the distribution of discadenine in different species of cellular slime molds by high-pressure liquid chromatography showed that discadenine is present in...

journal_title：Journal of bacteriology

authors： Taya Y,Yamada T,Nishimura S

更新日期：1980-08-01 00:00:00

abstract：:Streptococcus faecalis strain JH1 harbors two conjugative plasmids: pJH1, an R plasmid mediating resistance to kanamycin, streptomycin, gentamicin, erythromycin, and tetracycline, and pJH2, a hemolysin-bacteriocin plasmid. Studies of plasmid-cured derivatives of strain JH1 and of transconjugates obtained after mixed i...

journal_title：Journal of bacteriology

authors： LeBlanc DJ,Lee LN

更新日期：1982-05-01 00:00:00

abstract：:Dominance tests in diploids have confirmed that the product of the tyrR gene is involved in a negative control system affecting the synthesis of both 3-deoxy-d-arabinoheptulosonic acid 7-phosphate (DAHP) synthetase (tyr) and DAHP synthetase (phe). Some tyrR mutants are derepressed for the synthesis of both DAHP synthe...

journal_title：Journal of bacteriology

authors： Im SW,Pittard J

更新日期：1973-09-01 00:00:00