Autolysis of microbial cells: salt activation of autolytic enzymes in a mutant of Staphylococcus aureus.

Abstract:

:The effect of various salts on the autolysis of cell wall of a ribitol teichoic acid-deficient mutant of Staphylococcus aureus H (strain 52A5 carrying tar-1) was compared with the parent strain. In the presence of high concentrations of certain salts such as 1.0 m NaCl, the mutant undergoes autolysis with the release of osmotically sensitive spheroplasts. The parent strain is not affected by these conditions. The stimulation of lysis is related to an activation of N-acylmuramyl-l-alanine amidase.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Gilpin RW,Chatterjee AN,Young FE

doi

10.1128/JB.111.1.272-283.1972

subject

Has Abstract

pub_date

1972-07-01 00:00:00

pages

272-83

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

111

pub_type

杂志文章
  • L-serine degradation in Escherichia coli K-12: a combination of L-serine, glycine, and leucine used as a source of carbon.

    abstract::Escherichia coli K-12 strain CU1008 cannot use L-serine as the sole carbon source, but it could use L-serine as an auxiliary carbon source with glucose, L-alanine, or pyruvate and could derive energy from L-serine to support oxygen uptake. CU1008 grew with L-serine if it was also provided with glycine and leucine. The...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.151.2.777-782.1982

    authors: Newman EB,Walker C

    更新日期:1982-08-01 00:00:00

  • Identification and characterization of the nifV-nifZ-nifT gene region from the filamentous cyanobacterium Anabaena sp. strain PCC 7120.

    abstract::The nifV and leuA genes, which encode homocitrate synthase and alpha-isopropylmalate synthase, respectively, were cloned from the filamentous cyanobacterium Anabaena sp. strain PCC 7120 by a PCR-based strategy. Since the N-terminal parts of NifV and LeuA from other bacteria are highly similar to each other, a single p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.9.2930-2937.1997

    authors: Stricker O,Masepohl B,Klipp W,Böhme H

    更新日期:1997-05-01 00:00:00

  • IF3-mediated suppression of a GUA initiation codon mutation in the recJ gene of Escherichia coli.

    abstract::A mutational change of the initiation codon to GUA was found to reduce, but not abolish, expression of the recJ gene of Escherichia coli. Specific mutations in translational initiation factor IF3 have been isolated as second-site suppressors of this GUA initiation codon mutation. One of these, infC135, with an arginin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.21.6705-6713.1997

    authors: Haggerty TJ,Lovett ST

    更新日期:1997-11-01 00:00:00

  • Evidence for the existence of an aerobic pathway for synthesis of monounsaturated fatty acids by Alcaligenes faecalis.

    abstract::Studies on the composition of total fatty acids of Alcaligenes faecalis harvested at different growth phases have been carried out. Ability of the organism to desaturate palmitic and stearic acid has also been tested. The organism contained palmitic (16:0), stearic (18:0), palmitoleic (16:1), cis-vaccenic (18:1), cycl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.114.2.618-624.1973

    authors: Ghaneker AS,Nair PM

    更新日期:1973-05-01 00:00:00

  • Glucose catabolism in two derivatives of a Rhizobium japonicum strain differing in nitrogen-fixing efficiency.

    abstract::Radiorespirometric and enzymatic analyses reveal that glucose-grown cells of Rhizobium japonicum isolates I-110 and L1-110, both derivatives of R. japonicum strain 3I1b110, possess an active tricarboxylic acid cycle and metabolize glucose by simultaneous operation of the Embden-Meyerhof-Parnas and Entner-Doudoroff pat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.131.1.179-187.1977

    authors: Mulongoy K,Elkan GH

    更新日期:1977-07-01 00:00:00

  • DNA synthesis and viability of a mutT derivative of Escherichia coli WP2 under conditions of amino acid starvation and relation to stationary-phase (adaptive) mutation.

    abstract::Escherichia coli WP2 bacteria with an ochre amino acid auxotrophy show no evidence of growth during the first few days after plating at densities above 10(8) on plates lacking the required amino acid. They lose viability for some days, and then a subpopulation recovers and there is cell turnover. At very low plating d...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.11.2906-2910.1998

    authors: Bridges BA,Ereira S

    更新日期:1998-06-01 00:00:00

  • Cloning and genetic characterization of Helicobacter pylori catalase and construction of a catalase-deficient mutant strain.

    abstract::The N-terminal sequence of a protein, originally described as an adhesin of Helicobacter pylori, was used in an oligonucleotide-based screening procedure of an H. pylori plasmid library in Escherichia coli. Five independent plasmid clones were isolated, all mapping to the same chromosomal region and encoding the H. py...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.23.6960-6967.1996

    authors: Odenbreit S,Wieland B,Haas R

    更新日期:1996-12-01 00:00:00

  • Genetic organization of the Escherichia coli K10 capsule gene cluster: identification and characterization of two conserved regions in group III capsule gene clusters encoding polysaccharide transport functions.

    abstract::Analysis of the Escherichia coli K10 capsule gene cluster identified two regions, regions 1 and 3, conserved between different group III capsule gene clusters. Region 1 encodes homologues of KpsD, KpsM, KpsT, and KpsE proteins, and region 3 encodes homologues of the KpsC and KpsS proteins. An rfaH mutation abolished K...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.7.2279-2285.1999

    authors: Clarke BR,Pearce R,Roberts IS

    更新日期:1999-04-01 00:00:00

  • Repair and antirepair DNA helicases in Helicobacter pylori.

    abstract::Orthologs of RecG and RuvABC are highly conserved among prokaryotes; in Escherichia coli, they participate in independent pathways that branch migrate Holliday junctions during recombinational DNA repair. RecG also has been shown to directly convert stalled replication forks into Holliday junctions. The bacterium Heli...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01848-07

    authors: Kang J,Blaser MJ

    更新日期:2008-06-01 00:00:00

  • Evolutionary analysis of Burkholderia pseudomallei identifies putative novel virulence genes, including a microbial regulator of host cell autophagy.

    abstract::Burkholderia pseudomallei, the causative agent of melioidosis, contains a large pathogen genome (7.2 Mb) with ∼2,000 genes of putative or unknown function. Interactions with potential hosts and environmental factors may induce rapid adaptations in these B. pseudomallei genes, which can be discerned through evolutionar...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00718-13

    authors: Singh AP,Lai SC,Nandi T,Chua HH,Ooi WF,Ong C,Boyce JD,Adler B,Devenish RJ,Tan P

    更新日期:2013-12-01 00:00:00

  • Effect of D-amino acids on structure and synthesis of peptidoglycan in Escherichia coli.

    abstract::Growth of Escherichia coli in the presence of certain D-amino acids, such as D-methionine, results in the incorporation of the D-amino acid into macromolecular peptidoglycan and can be lethal at high concentrations. Previous studies suggested that incorporation was independent of the normal biosynthetic pathway. An en...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.17.5549-5559.1992

    authors: Caparrós M,Pisabarro AG,de Pedro MA

    更新日期:1992-09-01 00:00:00

  • Bacillus subtilis ccpA gene mutants specifically defective in activation of acetoin biosynthesis.

    abstract::A large number of carbon source utilization pathways are repressed in Bacillus subtilis by the global regulator CcpA, which also acts as an activator of carbon excretion pathways during growth in media containing glucose. In this study, CcpA mutants defective in transcriptional activation of the alsSD operon, which is...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.19.5611-5614.2000

    authors: Turinsky AJ,Moir-Blais TR,Grundy FJ,Henkin TM

    更新日期:2000-10-01 00:00:00

  • Purification and chemical composition of reticulate bodies of the meningopneumonitis organisms.

    abstract::Reticulate bodies of the meningopneumonitis (MP) microorganism were purified from L cells 18 hr after infection by the combination of differential centrifugation in 30% sucrose solution and potassium tartrate density gradient centrifugation. It was ascertained by electron microscopy that purified preparations of retic...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.6.2003-2008.1967

    authors: Tamura A,Matsumoto A,Higashi N

    更新日期:1967-06-01 00:00:00

  • Lysine biosynthesis in selected pathogenic fungi: characterization of lysine auxotrophs and the cloned LYS1 gene of Candida albicans.

    abstract::The alpha-aminoadipate pathway for the biosynthesis of lysine is present only in fungi and euglena. Until now, this unique metabolic pathway has never been investigated in the opportunistic fungal pathogens Candida albicans, Cryptococcus neoformans, and Aspergillus fumigatus. Five of the eight enzymes (homocitrate syn...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.22.7379-7384.1992

    authors: Garrad RC,Bhattacharjee JK

    更新日期:1992-11-01 00:00:00

  • The Importance of Being an Antagonist as Well as Persistent.

    abstract::The current work by Jain et al. (S. Jain, A. M. Chang, M. Singh, J. S. McLean, et al., J Bacteriol 201:e00683-18, 2019, https://doi.org/10.1128/JB.00683-18) reports the cloning of the lipid A deacylase gene of Porphyromonas gingivalis and the phenotypic characterization of the enzyme. Attempts to clone the gene and th...

    journal_title:Journal of bacteriology

    pub_type: 评论,杂志文章

    doi:10.1128/JB.00146-19

    authors: Progulske-Fox A

    更新日期:2019-05-08 00:00:00

  • Requirement for a functional host cell autolytic enzyme system for lysis of Escherichia coli by bacteriophage phi X174.

    abstract::Escherichia coli VC30 is a temperature-sensitive mutant which is defective in autolysis. Strain VC30 lyses at 30 degrees C when treated with beta-lactam antibiotics or D-cycloserine or when deprived of diaminiopimelic acid. The same treatments inhibit growth of the mutant at 42 degrees C but do not cause lysis. Strain...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.159.1.385-387.1984

    authors: Lubitz W,Harkness RE,Ishiguro EE

    更新日期:1984-07-01 00:00:00

  • An ATP-dependent L-carnitine transporter in Listeria monocytogenes Scott A is involved in osmoprotection.

    abstract::Listeria monocytogenes is a gram-positive, psychotrophic, food-borne pathogen which is able to grow in osmotically stressful environments. Carnitine (beta-hydroxy-L-tau-N-trimethyl aminobutyrate) can contribute significantly to growth of L. monocytogenes at high osmolarity (R. R. Beumer, M. C. te Giffel, L. J. Cox, F....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.11.3205-3212.1995

    authors: Verheul A,Rombouts FM,Beumer RR,Abee T

    更新日期:1995-06-01 00:00:00

  • An internal region of rpoB is required for autogenous translational regulation of the beta subunit of Escherichia coli RNA polymerase.

    abstract::In order to delineate the region involved in feedback regulation of the RNA polymerase beta subunit (encoded by rpoB), a collection of rpoB-lacZ translational fusions with different endpoints both upstream and downstream of the rpoB start site was assembled on lambda phage vectors. The extent of translational repressi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.22.7174-7179.1992

    authors: Passador L,Linn T

    更新日期:1992-11-01 00:00:00

  • Motility and chemotaxis of Spirochaeta aurantia: computer-assisted motion analysis.

    abstract::A computer program has been designed to study behavior in populations of Spirochaeta aurantia cells, and this program has been used to analyze changes in behavior in response to chemoattractants. Three kinds of behavior were distinguished: smooth swimming, flexing, and reversals in direction of swimming after a short ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.4.1768-1774.1988

    authors: Fosnaugh K,Greenberg EP

    更新日期:1988-04-01 00:00:00

  • The flat-ribbon configuration of the periplasmic flagella of Borrelia burgdorferi and its relationship to motility and morphology.

    abstract::Electron cryotomography was used to analyze the structure of the Lyme disease spirochete, Borrelia burgdorferi. This methodology offers a new means for studying the native architecture of bacteria by eliminating the chemical fixing, dehydration, and staining steps of conventional electron microscopy. Using electron cr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01288-08

    authors: Charon NW,Goldstein SF,Marko M,Hsieh C,Gebhardt LL,Motaleb MA,Wolgemuth CW,Limberger RJ,Rowe N

    更新日期:2009-01-01 00:00:00

  • Induction of heat shock proteins by abnormal proteins results from stabilization and not increased synthesis of sigma 32 in Escherichia coli.

    abstract::Accumulation of abnormal proteins in cells of bacteria or eukaryotes can induce synthesis of a set of heat shock proteins. We examined such induction following addition of azetidine (a proline analog) or synthesis of a heterologous protein (human prourokinase) in Escherichia coli. Synthesis of heat shock proteins unde...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.18.5648-5653.1994

    authors: Kanemori M,Mori H,Yura T

    更新日期:1994-09-01 00:00:00

  • Overproduction of Escherichia coli integration host factor, a protein with nonidentical subunits.

    abstract::Integration host factor (IHF) is a small, basic protein that is needed for efficient recombination of bacteriophage lambda, as well as for other host and viral functions. We have constructed strains in which the two subunits of IHF, encoded by the himA and hip genes of Escherichia coli, are expressed under the control...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.9.4124-4127.1987

    authors: Nash HA,Robertson CA,Flamm E,Weisberg RA,Miller HI

    更新日期:1987-09-01 00:00:00

  • Regulation of the adhE gene, which encodes ethanol dehydrogenase in Escherichia coli.

    abstract::The respiratory control of adhE, which encodes ethanol (alcohol) dehydrogenase in Escherichia coli, was examined at the transcriptional level by using various phi (adhE'-lacZ) adhE+ merodiploid strains. Expression of the adhE'-lacZ operon fusion was increased about eightfold by anaerobic growth. Under anaerobic growth...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.24.8009-8013.1991

    authors: Chen YM,Lin EC

    更新日期:1991-12-01 00:00:00

  • Cloning and analysis of the Bacillus subtilis rpsD gene, encoding ribosomal protein S4.

    abstract::The rpsD gene, encoding ribosomal protein S4, was isolated from Bacillus subtilis by hybridization with oligonucleotide probes derived from the S4 amino-terminal protein sequence. Sequence analysis of the cloned DNA indicated that rpsD is likely to be monocistronic, in contrast to Escherichia coli rpsD, which is locat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.11.6372-6379.1990

    authors: Grundy FJ,Henkin TM

    更新日期:1990-11-01 00:00:00

  • Surveying a supercoil domain by using the gamma delta resolution system in Salmonella typhimurium.

    abstract::A genetic system was developed to investigate the supercoil structure of bacterial chromosomes. New res-carrying transposons were derived from MudI1734 (MudJr1 and MudJr2) and Tn10 (Tn10dGn). The MudJr1 and MudJr2 elements each have a res site in opposite orientation so that when paired with a Tn10dGn element in the s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.10.2825-2835.1996

    authors: Higgins NP,Yang X,Fu Q,Roth JR

    更新日期:1996-05-01 00:00:00

  • Isolation of a metabolite capable of differentially supporting the growth of nicotinamide adenine dinucleotide auxotrophs of Escherichia coli.

    abstract::A compound, isolated from the culture fluid of a nadC auxotroph of Escherichia coli grown in a minimal medium, supports the growth of both a nadA and a nadB mutant. This metabolite exhibits an ultraviolet light absorption spectrum and a mass spectrum, different from quinolinic acid. This compound may be the precursor ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.121.1.212-218.1975

    authors: Chen JL,Tritz GJ

    更新日期:1975-01-01 00:00:00

  • Biochemical and genetic characterization of 2-carboxybenzaldehyde dehydrogenase, an enzyme involved in phenanthrene degradation by Nocardioides sp. strain KP7.

    abstract::2-Carboxybenzaldehyde dehydrogenase from the phenanthrene-degrading bacterium Nocardioides sp. strain KP7 was purified and characterized. The purified enzyme had a molecular mass of 53 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 205 kDa by gel filtration chromatography. Thus, the homotetramer ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.20.6488-6494.1997

    authors: Iwabuchi T,Harayama S

    更新日期:1997-10-01 00:00:00

  • Resistance of Salmonella typhimurium mutants to galactose death.

    abstract::A class of galactose-resistant mutants has been derived from strains of Salmonella typhimurium which are defective in uridine diphosphoglucose-4-epimerase. Resistant strains are phenotypically similar to parent organisms but do not lyse in the presence of galactose. Low levels of functional epimerase can be detected i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.1.103-109.1969

    authors: Shuster CW,Rundell K

    更新日期:1969-10-01 00:00:00

  • Toxicity of Pasteurella tularensis killed by ionizing radiation.

    abstract::Approximately 2 x 10(11) viable Pasteurella tularensis cells per ml, contained in suspensions, were killed by exposure to 10(6) r of gamma-radiation. When injected intraperitoneally into mice, the irradiated suspensions initially contained about 10 ld(50) per ml, and immunized mice against challenge with fully virulen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Landay ME,Wright GG,Pulliam JD,Finegold MJ

    更新日期:1968-09-01 00:00:00

  • Structure and composition of the cell wall of Neurospora crassa.

    abstract::The structure and composition of the cell walls of hyphae of Neurospora crassa were investigated by electron microscopy, chemical analysis, and X-ray diffraction both before and after progressive enzymatic degradation by snail gut enzymes, chitinase, and trypsin. The wall consists of two phases: randomly disposed skel...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.1.202-212.1967

    authors: Manocha MS,Colvin JR

    更新日期:1967-07-01 00:00:00