Abstract:
:In order to delineate the region involved in feedback regulation of the RNA polymerase beta subunit (encoded by rpoB), a collection of rpoB-lacZ translational fusions with different endpoints both upstream and downstream of the rpoB start site was assembled on lambda phage vectors. The extent of translational repression of beta was monitored by measuring beta-galactosidase levels in monolysogens of the fusions under conditions of increased intracellular concentrations of beta and beta' achieved via the induction of rpoBC expression from a multicopy plasmid. A construct containing as little as 29 bp upstream of the start of rpoB exhibited repression of beta-galactosidase activity to the same extent as a construct encoding the full upstream region. A construct which carried only 70 bp of the rpoB structural gene exhibited very little repression, while constructs which carried 126 or 221 bp of rpoB exhibited approximately the same degree of repression as a construct which carried 403 bp. These data suggest that the sequences important for feedback regulation of beta translation extend more than 70 bp into rpoB but are completely contained within a region which spans the sequences from 29 bp upstream to 126 bp downstream of the translational start site.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Passador L,Linn Tdoi
10.1128/jb.174.22.7174-7179.1992keywords:
subject
Has Abstractpub_date
1992-11-01 00:00:00pages
7174-9issue
22eissn
0021-9193issn
1098-5530journal_volume
174pub_type
杂志文章abstract::Synthetic analogs of enterochelin (enterobactin) were tested for their ability to support the growth of Escherichia coli K-12 under iron-limiting conditions. The cyclic compound MECAM [1,3,5-N.N'; N"-tris-(2,3-dihydroxybenzoyl)-triamino-methylbenzene] and its N-methyl derivative Me3MECAM promoted growth, whereas the 2...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.1.109-115.1983
更新日期:1983-01-01 00:00:00
abstract::TolB is a periplasmic protein of the cell envelope Tol complex. It is partially membrane associated through an interaction with the outer membrane lipoprotein PAL (peptidoglycan-associated lipoprotein), which also belongs to the Tol system. The interaction of TolB with outer membrane porins of Escherichia coli was inv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.23.7274-7279.1997
更新日期:1997-12-01 00:00:00
abstract::The genetic organization of the polypeptides required for the biosynthesis of the F41 adhesin of enterotoxigenic Escherichia coli strains was investigated. Maxicell analysis demonstrated that a recombinant plasmid which mediated mannose-resistant hemagglutination and F41 antigen production encoded four polypeptides of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.10.4890-4896.1988
更新日期:1988-10-01 00:00:00
abstract::The effects of competence-inducing treatments on the composition and organization of membrane lipids in Escherichia coli K-12, DH1, DH5, HB101, and RR1 were investigated for two widely used protocols in which transformability is developed at low temperatures in Ca2+ buffers. At stages during each procedure, the lipid ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.2.553-562.1986
更新日期:1986-11-01 00:00:00
abstract::We have identified and characterized enzymes of phospholipid synthesis in two plasmalogen-rich anaerobes. Megasphaera elsdenii and Veillonella parvula, and one anaerobe lacking plasmalogens. Desulfovibrio vulgaris. All three species contained phosphatidate cytidylyltransferase and phosphatidylserine synthase. Phosphat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.147.1.57-61.1981
更新日期:1981-07-01 00:00:00
abstract::The highly defective rho-15 mutant of Escherichia coli produces plasmid DNA that is 22% less negatively supercoiled than DNA from an isogenic wild-type strain (J. S. Fassler, G. F. Arnold, and I. Tessman, Mol. Gen. Genet. 204:424-429, 1986). We extended our measurements of plasmid superhelicity to additional rho mutan...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.9.4266-4271.1988
更新日期:1988-09-01 00:00:00
abstract::The nickel-cobalt-cadmium resistance genes carried by plasmid pTOM9 of Alcaligenes xylosoxidans 31A are located on a 14.5-kb BamHI fragment. By random Tn5 insertion mutagenesis, the fragment was shown to contain two distinct nickel resistance loci, ncc and nre. The ncc locus causes a high-level combined nickel, cobalt...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.22.7045-7054.1994
更新日期:1994-11-01 00:00:00
abstract::Gene 1 of bacteriophage T4 has been cloned into a lambda pL expression vector, resulting in the overproduction of deoxynucleotide kinase. A procedure that includes affinity chromatography on Cibacron Blue F3GA-agarose has been used to purify milligram quantities of enzymes from a 2-liter culture. The enzyme has been p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.6.2935-2939.1990
更新日期:1990-06-01 00:00:00
abstract::In stationary phase, 95% of the fructosyltransferase (FTase) activity of Streptococcus salivarius ATCC 25975 was found associated with the cells. Within the first 15 min after inoculation into fresh medium, the specific activity of cell-associated FTase decreased by 92% of its initial value. After this period of initi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.148.3.912-918.1981
更新日期:1981-12-01 00:00:00
abstract::Maleylacetate reductase (EC 1.3.1.32) plays a major role in the degradation of chloroaromatic compounds by channelling maleylacetate and some chlorinated derivatives into the 3-oxoadipate pathway. Several substituted maleylacetates were prepared in situ by alkaline or enzymatic hydrolysis of dienelactones as the precu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.2.320-325.1995
更新日期:1995-01-01 00:00:00
abstract::Upon exposure to alkylating agents, Escherichia coli increases expression of aidB along with three genes (ada, alkA, and alkB) that encode DNA repair proteins. While the biological roles of the Ada, AlkA, and AlkB proteins have been defined, despite many efforts, the molecular functions of AidB remain largely unknown....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00858-10
更新日期:2010-12-01 00:00:00
abstract::Mutants of Escherichia coli were isolated by their resistance to the bacteriocidal effects of the membrane-active drugs polymyxin B, levallorphan, and tetracaine. The mutants were examined for additional changes in cellular physiology evoked by the lesions; many polymyxin-resistant strains had a concomitant increased ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.127.2.961-972.1976
更新日期:1976-08-01 00:00:00
abstract::Ferric reductase activity was examined in Azotobacter vinelandii and was found to be located in the cytoplasm. The specific activities of soluble cell extracts were not affected by the iron concentration of the growth medium; however, activity was inhibited by the presence of Zn2+ during cell growth and also by the ad...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.7.4031-4037.1989
更新日期:1989-07-01 00:00:00
abstract::The so-called symbiotic region of the Bradyrhizobium japonicum chromosome (C. Kündig, H. Hennecke, and M. Göttfert, J. Bacteriol. 175:613-622, 1993) was screened for the presence of genes controlled by the nitrogen fixation regulatory protein NifA. Southern blots of restriction enzyme-digested cosmids that represent a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.6.1472-1480.2000
更新日期:2000-03-01 00:00:00
abstract::The inhibitory effect of fungitoxic derivatives of 1,4-oxathiin on substrate oxidation by the basidiomycete Ustilago maydis is diminished by a single-gene mutation (oxr). The difference between mutant and wild type is approximately the same on the basis of inhibition of either growth and operation of the tricarboxylic...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.110.3.809-817.1972
更新日期:1972-06-01 00:00:00
abstract:UNLABELLED:The gene encoding a nonoxidative decarboxylase capable of catalyzing the transformation of 2-hydroxy-1-naphthoic acid (2H1NA) to 2-naphthol was identified, recombinantly expressed, and purified to homogeneity. The putative gene sequence of the decarboxylase (hndA) encodes a 316-amino-acid protein (HndA) with...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00250-16
更新日期:2016-05-27 00:00:00
abstract::The regulatory properties of acetohydroxy acid synthetase (AHAS), the first enzyme in the biosynthetic pathway to valine and the second in the isoleucine pathway, were investigated in the fission yeast Schizosaccharomyces pombe. The enzyme was partially purified from crude extracts by protamine sulfate treatment, ammo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.1.332-340.1973
更新日期:1973-04-01 00:00:00
abstract::The nucleotide sequence of a 23S rRNA gene of Campylobacter coli VC167 was determined. The primary sequence of the C. coli 23S rRNA was deduced, and a secondary-structure model was constructed. Comparison with Escherichia coli 23S rRNA showed a major difference in the C. coli rRNA at approximately position 1170 (E. co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.15.4597-4609.1994
更新日期:1994-08-01 00:00:00
abstract::A thermophilic, sporeforming bacterium has been isolated from soil on a medium containing acetate as a carbon source. This organism is similar to Bacillus stearothermophilus in most respects but differs in its inability to hydrolyze starch. Isocitrate lyase is present in cell-free extracts of organisms grown in a medi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.2.703-710.1967
更新日期:1967-02-01 00:00:00
abstract::Horizontal acquisition of novel chromosomal genes is considered to be a key process in the evolution of bacterial pathogens. However, the identification of gene presence or absence could be hindered by the inconsistencies in bacterial genome annotations. Here, we performed a cross-annotation of omnipresent core and mo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00545-16
更新日期:2016-11-04 00:00:00
abstract::Bacillus anthracis Sterne cured of the pXO1 plasmid had enhanced secreted protease activity during the postexponential phase but no change in hemolytic or lecithinase activities. A zymogen profile revealed at least six proteases, including serine, metal, and perhaps cysteine types. There were similar amounts of protea...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.9.3133-3138.2005
更新日期:2005-05-01 00:00:00
abstract::Bacteriocin 28b from Serratia marcescens binds to Escherichia coli outer membrane proteins OmpA and OmpF and to lipopolysaccharide (LPS) core (J. Enfedaque, S. Ferrer, J. F. Guasch, J. Tomás, and M. Requé, Can. J. Microbiol. 42:19-26, 1996). A cosmid-based genomic library of S. marcescens was introduced into E. coli N...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.19.5741-5747.1996
更新日期:1996-10-01 00:00:00
abstract::A deoxyribonucleic acid-directed in vitro system for the synthesis of Escherichia coli carbamoylphosphate synthase has been developed, and its properties have been studied. The system uses the deoxyribonucleic acid of a lambda phage carrying the car genes (lambdadcarAB) as template and mediates the synthesis of both s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.1.58-66.1980
更新日期:1980-01-01 00:00:00
abstract::Proteus mirabilis is a dimorphic bacterium which exists in liquid cultures as a 1.5- to 2.0-microns motile swimmer cell possessing 6 to 10 peritrichous flagella. When swimmer cells are placed on a surface, they differentiate by a combination of events that ultimately produce a swarmer cell. Unlike the swimmer cell, th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.19.6279-6288.1991
更新日期:1991-10-01 00:00:00
abstract::Recent studies of anaerobic toluene catabolism have demonstrated a novel reaction for anaerobic hydrocarbon activation: the addition of the methyl carbon of toluene to fumarate to form benzylsuccinate. In vitro studies of the anaerobic benzylsuccinate synthase reaction indicate that the H atom abstracted from the tolu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.20.5454-5457.1998
更新日期:1998-10-01 00:00:00
abstract::Lysogenization of Salmonella typhimurium with either of the bacteriophages A3 and A4 results in O-acetylation of the L-rhamnose residues of the O-polysaccharide chain of the lipopolysaccharide of the bacterial cell envelope. The O-acetyl group is found on both O-2 and O-3 of the L-rhamnosyl residues. This lysogenic co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.3.1003-1009.1987
更新日期:1987-03-01 00:00:00
abstract::Rhizobia form a symbiotic relationship with plants of the legume family to produce nitrogen-fixing root nodules under nitrogen-limiting conditions. We have examined the importance of glutathione (GSH) during free-living growth and symbiosis of Sinorhizobium meliloti. An S. meliloti mutant strain (SmgshA) which is unab...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.1.168-174.2005
更新日期:2005-01-01 00:00:00
abstract::Interest in finding small RNAs (sRNAs) in bacteria has significantly increased in recent years due to their regulatory functions. Development of high-throughput methods and more sophisticated computational algorithms has allowed rapid identification of sRNA candidates in different species. However, given their various...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02359-14
更新日期:2015-01-01 00:00:00
abstract::Temperature induction of an Escherichia coli strains with lambda cI1857 integrated in the guaB gene has been used to produce strains containing chromosomal deletions extending into the xse and upp genes. By utilizing strains containing these deletions, it has been possible to order the genes in the guanine operon with...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.1.320-322.1979
更新日期:1979-07-01 00:00:00
abstract::Although yeast cannot normally incorporate exogenous deoxythymidine 5'-monophosphate (dTMP) into deoxyribonucleic acid, mutants able to do so have been isolated. We have characterized a recessive suppressor of dTMP uptake (sot1) that prevents strains carrying either tup1, tup2, or tup4 from growing on selective medium...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.2.638-641.1979
更新日期:1979-05-01 00:00:00