Occurrence of isocitrate lyase in a thermophilic bacillus species.

Abstract:

:A thermophilic, sporeforming bacterium has been isolated from soil on a medium containing acetate as a carbon source. This organism is similar to Bacillus stearothermophilus in most respects but differs in its inability to hydrolyze starch. Isocitrate lyase is present in cell-free extracts of organisms grown in a medium with acetate as a carbon source. The specific activity was 400 times lower in extracts of organisms utilizing glucose as a carbon source. With crude extracts, enzyme activity was strongly stimulated by Mg(++), but cysteine and ethylenediaminetetraacetate had little effect. It appeared to be more heat-stable than the pure isocitrate lyase from Pseudomonas indigofera.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Daron HH

doi

10.1128/JB.93.2.703-710.1967

subject

Has Abstract

pub_date

1967-02-01 00:00:00

pages

703-10

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

93

pub_type

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