Involvement of a cytochrome P450 monooxygenase in thaxtomin A biosynthesis by Streptomyces acidiscabies.

abstract：:The enzymes LinB(UT) and LinB(MI) (LinB from Sphingobium japonicum UT26 and Sphingobium sp. MI1205, respectively) catalyze the hydrolytic dechlorination of β-hexachlorocyclohexane (β-HCH) and yield different products, 2,3,4,5,6-pentachlorocyclohexanol (PCHL) and 2,3,5,6-tetrachlorocyclohexane-1,4-diol (TCDL), respecti...

journal_title：Journal of bacteriology

authors： Okai M,Ohtsuka J,Imai LF,Mase T,Moriuchi R,Tsuda M,Nagata K,Nagata Y,Tanokura M

更新日期：2013-06-01 00:00:00

abstract：:The galP1 promoter is responsible for galactose-dependent, glucose-sensitive transcription of the galactose utilization operon of Streptomyces coelicolor and Streptomyces lividans. We describe the characterization of mutations that were positioned directly upstream of the apparent transcription start site of galP1 and...

journal_title：Journal of bacteriology

authors： Mattern SG,Brawner ME,Westpheling J

更新日期：1993-03-01 00:00:00

abstract：:Flac mutants have been isolated in Escherichia coli K-12 which carry dominant mutations resulting in insensitivity to transfer inhibition by the Fin(+) I-like plasmid R62. These mutants were still sensitive to transfer inhibition by the fin(+) F-like plasmid R100 and, conversely, FlactraO(-) and traP(-) mutants, which...

journal_title：Journal of bacteriology

authors： Willetts NS,Paranchych W

更新日期：1974-10-01 00:00:00

abstract：:Spontaneous mutants that were resistant to zinc were isolated from Alcaligenes eutrophus CH34 containing either the native plasmid pMOL28 or a derivative derepressed for its self-transfer, pMOL50. With the cured plasmid-free derivative of CH34, strain AE104, such mutants were not detected. The mutations, which were sh...

journal_title：Journal of bacteriology

authors： Collard JM,Provoost A,Taghavi S,Mergeay M

更新日期：1993-02-01 00:00:00

abstract：:Outer membrane alterations were characterized in spontaneous mutants of the Erwinia chrysanthemi 3937jRH, which were selected for resistance to bacteriophage phi EC2. All but one of the mutants analyzed were affected in their lipopolysaccharide (LPS) structure, lacking the entire heterogeneous region of apparent high ...

journal_title：Journal of bacteriology

authors： Schoonejans E,Expert D,Toussaint A

更新日期：1987-09-01 00:00:00

abstract：:This study investigated the apparent genetic redundancy in the biosynthesis of polyhydroxyalkanoates (PHAs) in the Rhodospirillum rubrum genome revealed by the occurrence of three homologous PHA polymerase genes (phaC1, phaC2, and phaC3). In vitro biochemical assays established that each gene product encodes PHA polym...

journal_title：Journal of bacteriology

authors： Jin H,Nikolau BJ

更新日期：2012-10-01 00:00:00

abstract：:Vibrio cholerae is a natural inhabitant of aquatic ecosystems. Some strains of V. cholerae can colonize the human host and cause cholera, a profuse watery diarrhea. The major pathogenicity factors and virulence regulators of V. cholerae are either encoded in mobile genetic elements acquired in the environment (e.g. pa...

journal_title：Journal of bacteriology

authors： Sakib SN,Reddi G,Almagro-Moreno S

更新日期：2018-03-26 00:00:00

abstract：:The bacterium Caulobacter crescentus undergoes an asymmetric cell division resulting in the formation of two different daughter cells, a motile swarmer cell and a nonmotile stalked cell. These two cell types differ in their program of gene expression, their ability to replicate DNA, and the physical properties of thei...

journal_title：Journal of bacteriology

authors： Rizzo MF,Shapiro L,Gober J

更新日期：1993-11-01 00:00:00

abstract：:The initiation process of deoxyribonucleic acid (DNA) replication in Escherichia coli has been studied using the thermoreversible dna initiation mutant E. coli HfrHl65/120/6 dna-252. This dna mutation was incorrectly classed as a dnaA mutation. Biochemical and genetic evidence suggests that the dna-252 mutant is a nov...

journal_title：Journal of bacteriology

authors： Zyskind JW,Smith DW

更新日期：1977-03-01 00:00:00

abstract：:Two replicons were isolated independently from different IncHI1 plasmids. One was isolated from R27, and a second was isolated from pIP522. We demonstrate, by DNA-DNA hybridization experiments, that these maintenance regions are different and that they are specific to, and carried by, all IncHI1 plasmids tested. In vi...

journal_title：Journal of bacteriology

authors： Gabant P,Newnham P,Taylor D,Couturier M

更新日期：1993-12-01 00:00:00

abstract：:The properties of the anthranilate synthetase complex and its separated subunits were compared in catalyzing the anthranilate synthetase reaction, chorismate + l-glutamine or NH(4) (+) --> anthranilate, and the transferase reaction, anthranilate + 5'-phosphorylribosyl-1-pyrophosphate --> phosphoribosyl anthranilate. I...

journal_title：Journal of bacteriology

authors： Ito J,Yanofsky C

更新日期：1969-02-01 00:00:00

abstract：:Polyamines are required for rapid phospholipid turnover in Escherichia coli growing in media of low osmolarity. ...

journal_title：Journal of bacteriology

authors： Munro GF,Bell CA

更新日期：1973-12-01 00:00:00

abstract：:The inner or cytoplasmic membrane fraction of the cell envelope of Escherichia coli was isolated by isopycnic centrifugation on sucrose gradients. The membrane proteins were analyzed by electrophoresis in sodium dodecyl sulfate-polyacrylamide gels (8.5%), and up to 56 bands were resolved. Different preparations gave v...

journal_title：Journal of bacteriology

authors： Spencer ME,Guest JR

更新日期：1974-03-01 00:00:00

abstract：:The interaction of Streptococcus pyogenes (group A streptococcus [GAS]) with its human host requires several surface proteins. In this study, we isolated mutations in a gene required for the surface localization of protein F by transposon mutagenesis of the M6 strain JRS4. This gene (srtA) encodes a protein homologous...

journal_title：Journal of bacteriology

authors： Barnett TC,Scott JR

更新日期：2002-04-01 00:00:00

abstract：:Screening of derivatives of Rhizobium etli KIM5s randomly mutagenized with mTn5SSgusA30 resulted in the identification of strain KIM-G1. Its rough colony appearance, flocculation in liquid culture, and Ndv(-) Fix(-) phenotype were indicative of a lipopolysaccharide (LPS) defect. Electrophoretic analysis of cell-associ...

journal_title：Journal of bacteriology

authors： Vinuesa P,Reuhs BL,Breton C,Werner D

更新日期：1999-09-01 00:00:00

abstract：:Levy, Stuart B. (Institut du Radium, Paris, France). Isologous interference with ultraviolet and X-ray irradiated bacteriophage T(2). J. Bacteriol. 87:1330-1338. 1964.-Qualitative and quantitative analysis of the interference capacity of an irradiated T(2) bacteriophage was made with ultraviolet and X-ray irradiation....

journal_title：Journal of bacteriology

authors： LEVY SB

更新日期：1964-06-01 00:00:00

abstract：:Nine natural isolates of Escherichia coli were examined, and the sequence of the entire 1,404 bases of the gnd gene (6-phosphogluconate dehydrogenase, EC 1.1.1.44) was determined. These isolates, along with E. coli K-12, constitute 10 strains for analysis. (The sequence of the E. coli K-12 gnd gene is known.) A total ...

journal_title：Journal of bacteriology

authors： Bisercić M,Feutrier JY,Reeves PR

更新日期：1991-06-01 00:00:00

abstract：:The toxR gene of Vibrio cholerae encodes a transcriptional activator required for the expression of the cholera toxin genes (ctxAB) and more than 15 other genes encoding secreted or membrane proteins. The latter group includes virulence genes involved in the biogenesis of the TCP pilus, the accessory colonization fact...

journal_title：Journal of bacteriology

authors： Parsot C,Mekalanos JJ

更新日期：1991-05-01 00:00:00

abstract：:Campbell, J. J. R. (The University of British Columbia, Vancouver, B.C., Canada), Loretta A. Hogg, and G. A. Strasdine. Enzyme distribution in Pseudomonas aeruginosa. J. Bacteriol. 83:1155-1160. 1962.-Previous studies on the distribution of enzymes in bacteria have indicated that, although individual enzymes were pred...

journal_title：Journal of bacteriology

authors： CAMPBELL JJ,HOGGLA,STRASDINE GA

更新日期：1962-05-01 00:00:00

abstract：:A segment of Escherichia coli DNA that contained a discontinuity of homology with Salmonella typhimurium DNA was isolated. The segment, 1,430 base pairs long, was derived from one end of the lac "loop," a region of about 12 kilobase pairs of E. coli DNA, including the lac operon which has no detectable homology with S...

journal_title：Journal of bacteriology

authors： Buvinger WE,Lampel KA,Bojanowski RJ,Riley M

更新日期：1984-08-01 00:00:00

abstract：:NBU1 is an integrated Bacteroides element that can he mobilized from Bacteroides donors to Bacteroides recipients. Previous studies have shown that a plasmid carrying the internal mobilization region of NBU1 could be transferred by conjugation from Bacteroides thetaiotaomicron to Escherichia coli. In this report, we s...

journal_title：Journal of bacteriology

authors： Shoemaker NB,Wang GR,Salyers AA

更新日期：1996-06-01 00:00:00

abstract：:Two new mutants of Rhodobacter sphaeroides deficient in sulfolipid accumulation were isolated by directly screening mutagenized cell lines for polar lipid composition by thin-layer chromatography of lipid extracts. A genomic clone which complemented the mutations in these two lines, but not the previously described su...

journal_title：Journal of bacteriology

authors： Benning C,Somerville CR

更新日期：1992-10-01 00:00:00

abstract：:The Tat system, found in the cytoplasmic membrane of many bacteria, is a general export pathway for folded proteins. Here we describe the development of a method, based on the transport of chloramphenicol acetyltransferase, that allows positive selection of mutants defective in Tat function. We have demonstrated the u...

journal_title：Journal of bacteriology

authors： Hicks MG,Lee PA,Georgiou G,Berks BC,Palmer T

更新日期：2005-04-01 00:00:00

abstract：:The sequencing of the EcoRI-HindIII fragment complementing mutations in the structural genes of the L-rhamnose regulon of Escherichia coli has permitted identification of the open reading frames corresponding to rhaB, rhaA, and rhaD. The deduced amino acid sequences gave a 425-amino-acid polypeptide corresponding to r...

journal_title：Journal of bacteriology

authors： Moralejo P,Egan SM,Hidalgo E,Aguilar J

更新日期：1993-09-01 00:00:00

abstract：:Hirota, Yukinori (University of Osaka, Osaka, Japan), Toshio Fujii, and Yukinobu Nishimura. Loss and repair of conjugal fertility and infectivity of the resistance factor and sex factor in Escherichia coli. J. Bacteriol. 91:1298-1304. 1966.-The drug-resistance factor, R, and the sex factor, F, have homologous traits, ...

journal_title：Journal of bacteriology

authors： Hirota Y,Fujii T,Nishimua Y

更新日期：1966-03-01 00:00:00

abstract：:The global regulator CsrA (carbon storage regulator) is an RNA binding protein that coordinates central carbon metabolism, activates flagellum biosynthesis and motility, and represses biofilm formation in Escherichia coli. CsrA activity is antagonized by the untranslated RNA CsrB, to which it binds and forms a globula...

journal_title：Journal of bacteriology

authors： Suzuki K,Wang X,Weilbacher T,Pernestig AK,Melefors O,Georgellis D,Babitzke P,Romeo T

更新日期：2002-09-01 00:00:00

abstract：:The cell envelope of gram-negative bacteria is responsible for many important biological functions: it plays a structural role, it accommodates the selective transfer of material across the cell wall, it undergoes changes made necessary by growth and division, and it transfers information about the environment into th...

journal_title：Journal of bacteriology

authors： Vadillo-Rodriguez V,Beveridge TJ,Dutcher JR

更新日期：2008-06-01 00:00:00

abstract：:ShdA is a large outer membrane protein of the autotransporter family whose passenger domain binds the extracellular matrix proteins fibronectin and collagen I, possibly by mimicking the host ligand heparin. The ShdA passenger domain consists of approximately 1,500 amino acid residues that can be divided into two regio...

journal_title：Journal of bacteriology

authors： Kingsley RA,Abi Ghanem D,Puebla-Osorio N,Keestra AM,Berghman L,Bäumler AJ

更新日期：2004-08-01 00:00:00

abstract：:Cells of Bacillus thuringiensis containing refractile spores autolyzed readily when suspended in buffer. The autolysate contained enzymes which lysed vegetative cell walls of the organism. Three enzymes were isolated from the autolysate, and each was purified approximately 30-fold. One enzyme, most active near pH 4.0,...

journal_title：Journal of bacteriology

authors： Kingan SL,Ensign JC

更新日期：1968-09-01 00:00:00

abstract：:The spoIIG promoter is used by RNA polymerase containing sigma A (E sigma A), the primary form of RNA polymerase found in vegetative cells in Bacillus subtilis. However, the spoIIG promoter is active only after the onset of sporulation. Activation of the spoIIG promoter requires the product of the spo0A gene (Spo0A). ...

journal_title：Journal of bacteriology

authors： Satola SW,Baldus JM,Moran CP Jr

更新日期：1992-03-01 00:00:00