Characterization and virulence properties of Erwinia chrysanthemi lipopolysaccharide-defective, phi EC2-resistant mutants.

abstract：:Proteins synthesized in Escherichia coli during recovery from starvation were resolved by two-dimensional polyacrylamide gel electrophoresis. Nine outgrowth-specific proteins, which appeared in two kinetic groups, that were not detected in either starved or exponential-phase cells were synthesized. Five other proteins...

journal_title：Journal of bacteriology

authors： Siegele DA,Guynn LJ

更新日期：1996-11-01 00:00:00

abstract：:Efficient hydrolysis of native poly(3-hydroxybutyrate) (nPHB) granules in vitro by soluble PHB depolymerase of Rhodospirillum rubrum requires pretreatment of nPHB with an activator compound present in R. rubrum cells (J. M. Merrick and M. Doudoroff, J. Bacteriol. 88:60-71, 1964). Edman sequencing of the purified activ...

journal_title：Journal of bacteriology

authors： Handrick R,Reinhardt S,Schultheiss D,Reichart T,Schüler D,Jendrossek V,Jendrossek D

更新日期：2004-04-01 00:00:00

abstract：:Amplification of the mraY gene, previously called open reading frame Y (ORF-Y, 1,080 bp), at 2 min in the chromosome map of Escherichia coli enhanced the activity of UDP-N-acetylmuramoyl-pentapeptide: undecaprenyl-phosphate phospho-N-acetylmuramoyl-pentapeptide transferase (EC 2.7.8.13). This enzyme catalyzes the form...

journal_title：Journal of bacteriology

authors： Ikeda M,Wachi M,Jung HK,Ishino F,Matsuhashi M

更新日期：1991-02-01 00:00:00

abstract：:Antiserum prepared against protocatechuate 3, 4-dioxygenase from Pseudomonas aeruginosa forms precipitin bands without spurs with isofunctional enzymes from different strains of the fluorescent pseudomonads on immunodiffusion plates. Catalytic activity of the isofunctional enzymes was inhibited by an immunoglobulin fr...

journal_title：Journal of bacteriology

authors： Hou CT,Lillard MO

更新日期：1976-04-01 00:00:00

abstract：:Multicopy single-stranded DNA is found as a small single-stranded RNA-DNA complex in certain wild-type strains of Escherichia coli as well as in other gram-negative bacteria. Using the promoter region of the previously characterized retron-Ec107 from E. coli ECOR70, I constructed a chromosomally located lacZ operon fu...

journal_title：Journal of bacteriology

authors： Herzer PJ

更新日期：1996-08-01 00:00:00

abstract：:The methylenetetrahydrofolate dehydrogenase of the amethopterin-resistant strain Streptococcus faecium var. durans A(k) was purified 100-fold. Because it is extremely labile, this enzyme required protection by 1 mm nicotinamide adenine dinucleotide phosphate (NADP(+)) during purification; 0.01 mm EADP(+) with 0.1% bov...

journal_title：Journal of bacteriology

authors： Albrecht AM,Pearce FK,Hutchison DJ

更新日期：1968-05-01 00:00:00

abstract：:Isolation of six strains of Escherichia coli K-12 carrying mutations affecting the citrate-dependent iron uptake system is described. Genetic analysis of these mutants showed that the mutation affecting the citrate system are cluster together at about min 6 on the E. coli chromosome. ...

journal_title：Journal of bacteriology

authors： Woodrow GC,Langman L,Young IG,Gibson F

更新日期：1978-03-01 00:00:00

abstract：:Proteus swarming is the rapid cyclical population migration across surfaces by elongated cells that hyperexpress flagellar and virulence genes. The mini-Tn5 transposon mutant mns2 was isolated as a tight nonswarming mutant that did not elongate or upregulate flagellar and hemolysin genes. Individual cell motility was ...

journal_title：Journal of bacteriology

authors： Hay NA,Tipper DJ,Gygi D,Hughes C

更新日期：1997-08-01 00:00:00

abstract：:Comamonas sp. strain CNB-1 degrades nitrobenzene and chloronitrobenzene via the intermediates 2-aminomuconate and 2-amino-5-chloromuconate, respectively. Deamination of these two compounds results in the release of ammonia, which is used as a source of nitrogen for bacterial growth. In this study, a novel deaminase wa...

journal_title：Journal of bacteriology

authors： Liu L,Wu JF,Ma YF,Wang SY,Zhao GP,Liu SJ

更新日期：2007-04-01 00:00:00

abstract：:Bacillus subtilis 168ts-200B is a temperature-sensitive mutant of B. subtilis 168 which grows as rods at 30 C but as irregular spheres at 45 C. Growth at the nonpermissive temperature resulted in a deficiency of teichoic acid in the cell wall. A decrease in teichoic acid synthesis coupled with the rapid turnover of th...

journal_title：Journal of bacteriology

authors： Boylan RJ,Mendelson NH,Brooks D,Young FE

更新日期：1972-04-01 00:00:00

abstract：:Bacillus subtilis produces a 35-kDa cell wall hydrolase, CwlF, during vegetative growth. The CwlF protein was extracted from B. subtilis cwlB sigD mutant cells and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. N-terminal amino acid sequencing revealed that its sequence is completely identical...

journal_title：Journal of bacteriology

authors： Ishikawa S,Hara Y,Ohnishi R,Sekiguchi J

更新日期：1998-05-01 00:00:00

abstract：:Welker, N. E. (University of Illinois, Urbana), and L. Leon Campbell. Biochemical changes in lysogenic Bacillus stearothermophilus after bacteriophage induction. J. Bacteriol. 90:1129-1137. 1965.-Cultures of Bacillus stearothermophilus 1503-4R (TP-1) continued to grow at an unaltered rate after induction with mitomyci...

journal_title：Journal of bacteriology

authors： Welker NE,Campbell LL

更新日期：1965-10-01 00:00:00

abstract：:The participation of tyramine oxidase in the regulation of arylsulfatase synthesis in Salmonella typhimurium was studied. Arylsulfatase synthesis was repressed by inorganic sulfate, cysteine, methionine, or taurine. This repression was relieved by tyramine, octopamine, or dopamine, which induced tyramine oxidase synth...

journal_title：Journal of bacteriology

authors： Murooka Y,Harada T

更新日期：1981-02-01 00:00:00

abstract：:Outer membranes, free of cytoplasmic or thylakoid membranes and peptidoglycan components, were obtained from Synechocystis sp. strain PCC6714. Electron microscope studies revealed double-track outer membrane vesicles with a smooth-appearing exoplasmic surface, an exoplasmic fracture face covered by closely packed part...

journal_title：Journal of bacteriology

authors： Jürgens UJ,Weckesser J

更新日期：1985-10-01 00:00:00

abstract：:In streptomycete anthracycline biosynthetic gene clusters, small open reading frames are located just upstream of minimal polyketide synthase genes. aknX is such a gene found in the aklavinone-aclacinomycin biosynthetic gene cluster of Streptomyces galilaeus. In order to identify its function, the aknX gene was expres...

journal_title：Journal of bacteriology

authors： Chung JY,Fujii I,Harada S,Sankawa U,Ebizuka Y

更新日期：2002-11-01 00:00:00

abstract：:De Repentigny, J. (University of Montreal, Montreal, Quebec, Canada), S. Sonea, and A. Frappier. Differentiation by immunodiffusion and by quantitative immunofluorescence between 5-fluorouracil-treated and normal cells from a toxinogenic Staphylococcus aureus strain. J. Bacteriol. 88:444-448. 1964.-Immunodiffusion and...

journal_title：Journal of bacteriology

authors： DEREPENTIGNY J,SONEA S,FRAPPIER A

更新日期：1964-08-01 00:00:00

abstract：:Escherichia coli K-12 produces at least two ATP-dependent proteases, Lon (La) and Clp (Ti), the latter consisting of a regulatory subunit (ClpA) and a proteolytic subunit (ClpP). The gene clpB encoding an analog of ClpA had been found at 57 min on the E. coli chromosome. Cloning and examination of novel heat shock pro...

journal_title：Journal of bacteriology

authors： Kitagawa M,Wada C,Yoshioka S,Yura T

更新日期：1991-07-01 00:00:00

abstract：:Construction and characterization of a class of multicopy plasmid cloning vehicles containing the replication system of miniplasmid P15A are described. The constructed plasmids have cleavage sites within antibiotic resistance genes for a variety of commonly employed site-specific endonucleases, permitting convenient u...

journal_title：Journal of bacteriology

authors： Chang AC,Cohen SN

更新日期：1978-06-01 00:00:00

abstract：:An exo-beta-1,4-glucanase (Exo A) from Ruminococcus flavefaciens FD-1 was purified to homogeneity and characterized. Enzyme activity was monitored during purification by using the substrate p-nitrophenyl-beta-D-cellobioside (NPC). Over 85% of the NPC activity was found to be extracellular once the filter paper was deg...

journal_title：Journal of bacteriology

authors： Gardner RM,Doerner KC,White BA

更新日期：1987-10-01 00:00:00

abstract：:Purified bulk tRNA from Methanococcus vanielii (carbon source, formate) showed variation in the modified nucleoside pattern reported for Escherichia coli as analyzed by both ion-exchange and thin-layer chromatography. Ribothymidine and 7-methylguanosine were absent; 1-methyladenosine, 1-methylguanosine, N2-methylguano...

journal_title：Journal of bacteriology

authors： Best AN

更新日期：1978-01-01 00:00:00

abstract：:The genes encoding the two isoenzymes of methyl coenzyme M reductase (MRI and MRII) in Methanobacterium thermoautotrophicum delta H have been cloned and sequenced. The MRI-encoding mcr operon (mcrBDCGA) has been located immediately upstream from the mtr operon (mtrEDCBA) that encodes N5-methyltetrahydromethanopterin:c...

journal_title：Journal of bacteriology

authors： Pihl TD,Sharma S,Reeve JN

更新日期：1994-10-01 00:00:00

abstract：:Addition of casein hydrolysate to suspensions of washed, nonpigmented, nonproliferating Serratia marcescens incubating at 27 C induced biosynthesis of prodigiosin. Four amino acids of casein hydrolysate, dl-aspartic acid, l-glutamic acid, l-proline, and l-alanine caused formation of pigment when added individually. dl...

journal_title：Journal of bacteriology

authors： Williams RP,Gott CL,Qadri SM

更新日期：1971-05-01 00:00:00

abstract：:Conditions for continuous culture of Escherichia coli K-12 His- Thi- under glucose limitation were established. Both the capacity for respiration, at D greater than 0.2/h, and specific activity of superoxide dismutase increased as a function of specific growth rate, whereas peroxidase and catalase were either invarian...

journal_title：Journal of bacteriology

authors： Hassan HM,Fridovich I

更新日期：1977-05-01 00:00:00

abstract：:Purified L-asparaginase from Serratia marcescens had an apparent-weight average molecular weight of 171,000 to 180,000 as determined by electrophoresis on polyacrylamide gels and by sedimentation equilibrium at low speed in an analytical ultracentrifuge. A subunit molecular weight of 31,500 +/- 1,500 was estimated for...

journal_title：Journal of bacteriology

authors： Stern ML,Phillips AW,Gottlieb AJ

更新日期：1976-02-01 00:00:00

abstract：:The distribution of the extracellular enzyme, thiaminase I, was determined for logarithmically growing cultures of Bacillus thiaminolyticus. About 60% of the enzyme is associated with the cells throughout the growth cycle. The remainder of the enzyme is in the culture medium. The release of the cell-bound thiaminase I...

journal_title：Journal of bacteriology

authors： Agee CC,Wilkins JH,Airth RL

更新日期：1973-09-01 00:00:00

abstract：:The opportunistic pathogen Pseudomonas aeruginosa utilizes a type III secretion system (T3SS) to evade phagocytosis and damage eukaryotic cells. Transcription of the T3SS regulon is controlled by ExsA, a member of the AraC/XylS family of transcriptional regulators. These family members generally consist of an approxim...

journal_title：Journal of bacteriology

authors： Brutinel ED,Vakulskas CA,Yahr TL

更新日期：2009-06-01 00:00:00

abstract：:The enzyme nicotinamide mononucleotide (NMN) adenylyltransferase (EC 2.7.7.1) catalyzes the synthesis of NAD+ and nicotinic acid adenine dinucleotide. It has been purified to homogeneity from cellular extracts of the thermophilic archaeon Sulfolobus solfataricus. Through a database search, a highly significant match w...

journal_title：Journal of bacteriology

authors： Raffaelli N,Pisani FM,Lorenzi T,Emanuelli M,Amici A,Ruggieri S,Magni G

更新日期：1997-12-01 00:00:00

abstract：:The par stability determinant, encoded by the Enterococcus faecalis plasmid pAD1, is the only antisense RNA regulated postsegregational killing system identified in gram-positive bacteria. Because of the unique organization of the par locus, the par antisense RNA, RNA II, binds to its target, RNA I, at relatively smal...

journal_title：Journal of bacteriology

authors： Weaver KE,Ehli EA,Nelson JS,Patel S

更新日期：2004-10-01 00:00:00

abstract：:The Escherichia coli F plasmid gene required for amino-terminal acetylation of F-pilin subunits was identified. Using Western blots (immunoblots), we assayed the reaction of monoclonal antibodies with F-pilin polypeptides in inner membrane preparations from various F mutant strains. It was known that JEL92 recognizes ...

journal_title：Journal of bacteriology

authors： Moore D,Hamilton CM,Maneewannakul K,Mintz Y,Frost LS,Ippen-Ihler K

更新日期：1993-03-01 00:00:00

abstract：:The two 23S rRNA-5S rRNA-tRNAGly operons from the extreme thermophilic eubacterium Thermus thermophilus HB8 were used to characterized the in vivo processing and termination of 23S rRNA-5S rRNA-tRNAGly primary transcripts in this organism by nuclease S1 mapping. A processing site in the pre-23S rRNA 3'-flanking region...

journal_title：Journal of bacteriology

authors： Hartmann RK,Toschka HY,Erdmann VA

更新日期：1991-04-01 00:00:00