Abstract:
:Amplification of the mraY gene, previously called open reading frame Y (ORF-Y, 1,080 bp), at 2 min in the chromosome map of Escherichia coli enhanced the activity of UDP-N-acetylmuramoyl-pentapeptide: undecaprenyl-phosphate phospho-N-acetylmuramoyl-pentapeptide transferase (EC 2.7.8.13). This enzyme catalyzes the formation of undecaprenyl-pyrophosphoryl-N-acetylmuramoyl-pentapeptide from UDP-N-acetylmuramoyl-pentapeptide and undecaprenyl-phosphate, the first step in the lipid cycle reactions in biosynthesis of bacterial cell wall peptidoglycans. The enhanced enzyme activity was sensitive to tunicamycin, and the amino tunicamycin-sensitive N-acetylglucosamine-1-phosphate transferase of Saccharomyces cerevisiae. Very probably mraY is the structural gene for the above enzyme.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Ikeda M,Wachi M,Jung HK,Ishino F,Matsuhashi Mdoi
10.1128/jb.173.3.1021-1026.1991subject
Has Abstractpub_date
1991-02-01 00:00:00pages
1021-6issue
3eissn
0021-9193issn
1098-5530journal_volume
173pub_type
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更新日期:1987-10-01 00:00:00
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