Cell-free amino acid-incorporating system from Pseudomonas indigofera.

abstract：:The Escherichia coli gene cluster encoding enzymatic activities responsible for the synthesis and activation of 2,3-dihydroxybenzoic acid in the formation of the catechol siderophore enterobactin was localized to a 4.2-kilobase chromosomal DNA fragment. Analysis of various subclones and transposon insertion mutations ...

journal_title：Journal of bacteriology

authors： Nahlik MS,Fleming TP,McIntosh MA

更新日期：1987-09-01 00:00:00

abstract：:Heating competent Azotobacter vinelandii at 37 or 42 degrees C resulted in a total loss of competence with no loss of viability. The transformation process was relatively insensitive to heating at either temperature once DNase-resistant DNA binding was nearly complete. Although competent and 42 degrees C-treated cells...

journal_title：Journal of bacteriology

authors： Doran JL,Page WJ

更新日期：1983-07-01 00:00:00

abstract：:S-Adenosyl-L-methionine:precorrin-2 methyltransferase (SP2MT), which catalyzes the C-20 methylation of precorrin-2 to precorrin-3, was purified to homogeneity from extracts of a recombinant strain of Pseudomonas denitrificans derived from a cobalamin-overproducing strain. Ammonium sulfate fractionation followed by chr...

journal_title：Journal of bacteriology

authors： Thibaut D,Couder M,Crouzet J,Debussche L,Cameron B,Blanche F

更新日期：1990-11-01 00:00:00

abstract：:The complete Bacillus subtilis genome contains two genes with the potential to encode glutamate dehydrogenase (GlutDH) enzymes. Mutations in these genes were constructed and characterized. The rocG gene proved to encode a major GlutDH whose synthesis was induced in media containing arginine or ornithine or, to a lesse...

journal_title：Journal of bacteriology

authors： Belitsky BR,Sonenshein AL

更新日期：1998-12-01 00:00:00

abstract：:Microorganisms and plants utilize two-component systems to regulate adaptive responses to changing environmental conditions. Sensor kinases detect stimuli and alter their autophosphorylation activity accordingly. Signal propagation occurs via the transfer of phosphoryl groups from upstream kinases to downstream respon...

journal_title：Journal of bacteriology

authors： Straughn PB,Vass LR,Yuan C,Kennedy EN,Foster CA,Bourret RB

更新日期：2020-07-09 00:00:00

abstract：:The TetA(C) protein, encoded by the tetA(C) gene of plasmid pBR322, is a member of a family of membrane-bound proteins that mediate energy-dependent efflux of tetracycline from the bacterial cell. The tetA(C) gene was mutagenized with hydroxylamine, and missense mutations causing the loss of tetracycline resistance we...

journal_title：Journal of bacteriology

authors： McNicholas P,Chopra I,Rothstein DM

更新日期：1992-12-01 00:00:00

abstract：:IS630 is a 1.15-kilobase sequence in Shigella sonnei that, unlike many mobile elements, seems not to mediate cointegration between different replicons. To assess its transposition, we constructed composite elements containing inverted copies of IS630 flanking a drug resistance gene. We found that these composite eleme...

journal_title：Journal of bacteriology

authors： Tenzen T,Matsutani S,Ohtsubo E

更新日期：1990-07-01 00:00:00

abstract：:The Brucella abortus virB locus contains 12 open reading frames, termed virB1 through virB12, which encode a type IV secretion system. Polar mutations in the virB locus markedly reduce the ability of B. abortus to survive in cultured macrophages or to persist in organs of mice. While a nonpolar deletion of the virB2 g...

journal_title：Journal of bacteriology

authors： den Hartigh AB,Rolán HG,de Jong MF,Tsolis RM

更新日期：2008-07-01 00:00:00

abstract：:The gut microbiota plays a crucial role in the development of the immune system and confers benefits or disease susceptibility to the host. Emerging studies have indicated the gut microbiota could affect pulmonary health and disease through cross talk between the gut microbiota and the lungs. Gut microbiota dysbiosis c...

journal_title：Journal of bacteriology

authors： Zhou A,Lei Y,Tang L,Hu S,Yang M,Wu L,Yang S,Tang B

更新日期：2021-01-25 00:00:00

abstract：:Genetic circularity of the Pseudomonas aeruginosa PAO chromosome was demonstrated by a series of two- and three-factor crosses and double-selection experiments with Cma plasmids FP2, FP5, FP110, and R68.45. A range of additional markers, including catabolic markers, were located on the chromosome map. Plasmid FP2, kno...

journal_title：Journal of bacteriology

authors： Royle PL,Matsumoto H,Holloway BW

更新日期：1981-01-01 00:00:00

abstract：:Uptake of cobalamins and iron chelates in Escherichia coli K-12 is dependent on specific outer membrane transport proteins and the energy-coupling function provided by the TonB protein. The btuB product is the outer membrane receptor for cobalamins, bacteriophage BF23, and the E colicins. A short sequence near the ami...

journal_title：Journal of bacteriology

authors： Gudmundsdottir A,Bell PE,Lundrigan MD,Bradbeer C,Kadner RJ

更新日期：1989-12-01 00:00:00

abstract：:Preincubation of the blue-green alga (cyanobacterium) Nostoc muscorum under an atmosphere of argon plus acetylene in the light led to a greater than fourfold increase of light-induced hydrogen evolution and to a 50% increase of acetylene reduction, as compared to cells that had not been preconditioned. The basic and t...

journal_title：Journal of bacteriology

authors： Scherer S,Kerfin W,Böger P

更新日期：1980-03-01 00:00:00

abstract：:During Bacillus subtilis sporulation, the transient engulfment defect of spoIIB strains is enhanced by spoVG null mutations and suppressed by spoVS null mutations. These mutations have opposite effects on expression of the motility regulon, as the spoVG mutation reduces and the spoVS mutation increases sigmaD-directed...

journal_title：Journal of bacteriology

authors： Perez AR,Abanes-De Mello A,Pogliano K

更新日期：2006-02-01 00:00:00

abstract：:Under static growth conditions, hyperpiliated, nontwitching pilT and pilU mutants of Pseudomonas aeruginosa formed dense biofilms, showing that adhesion, not twitching motility, is necessary for biofilm initiation. Under flow conditions, the pilT mutant formed mushroom-like structures larger than those of the wild typ...

journal_title：Journal of bacteriology

authors： Chiang P,Burrows LL

更新日期：2003-04-01 00:00:00

abstract：:DNA polymerase activities in fractionated cell extract of Aeropyrum pernix, a hyperthermophilic crenarchaeote, were investigated. Aphidicolin-sensitive (fraction I) and aphidicolin-resistant (fraction II) activities were detected. The activity in fraction I was more heat stable than that in fraction II. Two different ...

journal_title：Journal of bacteriology

authors： Cann IK,Ishino S,Nomura N,Sako Y,Ishino Y

更新日期：1999-10-01 00:00:00

abstract：:Short sequence repeats (SSRs), potentially representing variable numbers of tandem repeat (VNTR) loci, were identified for the human-pathogenic yeast species Candida albicans by computerized DNA sequence scanning. The individual SSR regions were investigated in different clinical isolates of C. albicans. Most of the C...

journal_title：Journal of bacteriology

authors： Lunel FV,Licciardello L,Stefani S,Verbrugh HA,Melchers WJ,Meis JF,Scherer S,van Belkum A

更新日期：1998-08-01 00:00:00

abstract：:A novel sucrose hydrolase (SUH) from Xanthomonas axonopodis pv. glycines, a causative agent of bacterial pustule disease on soybeans, was studied at the functional and molecular levels. SUH was shown to act rather specifically on sucrose (K(m) = 2.5 mM) but not on sucrose-6-phosphate. Protein analysis of purified SUH ...

journal_title：Journal of bacteriology

authors： Kim HS,Park HJ,Heu S,Jung J

更新日期：2004-01-01 00:00:00

abstract：:Bacillus subtilis undergoes a highly distinctive division during spore formation. It yields two unequal cells, the mother cell and the prespore, and septum formation is completed before the origin-distal 70% of the chromosome has entered the smaller prespore. The mother cell subsequently engulfs the prespore. Two diff...

journal_title：Journal of bacteriology

authors： Bogush M,Xenopoulos P,Piggot PJ

更新日期：2007-05-01 00:00:00

abstract：:Radiorespirometric and enzymatic analyses reveal that glucose-grown cells of Rhizobium japonicum isolates I-110 and L1-110, both derivatives of R. japonicum strain 3I1b110, possess an active tricarboxylic acid cycle and metabolize glucose by simultaneous operation of the Embden-Meyerhof-Parnas and Entner-Doudoroff pat...

journal_title：Journal of bacteriology

authors： Mulongoy K,Elkan GH

更新日期：1977-07-01 00:00:00

abstract：:The intervals between rounds of chromosome and minichromosome replication were measured by density shift experiments and found to be similar. Thus the minichromosome, a lambda asnA oriC bacteriophage, mostly replicates once each division cycle rather than randomly, despite its high copy number. Slight differences betw...

journal_title：Journal of bacteriology

authors： Koppes LJ,von Meyenburg K

更新日期：1987-01-01 00:00:00

abstract：:The genetic organization of the polypeptides required for the biosynthesis of the F41 adhesin of enterotoxigenic Escherichia coli strains was investigated. Maxicell analysis demonstrated that a recombinant plasmid which mediated mannose-resistant hemagglutination and F41 antigen production encoded four polypeptides of...

journal_title：Journal of bacteriology

authors： Anderson DG,Moseley SL

更新日期：1988-10-01 00:00:00

abstract：:Streptococcus agalactiae cultures possess an aerobic pathway for glucose oxidation that is strongly inhibited by cyanide. The products of glucose oxidation by aerobically grown cells of S. agalactiae 50 are lactic and acetic acids, acetylmethylcarbinol, and carbon dioxide. Glucose degradation products by aerobically g...

journal_title：Journal of bacteriology

authors： Mickelson MN

更新日期：1967-07-01 00:00:00

abstract：:Cook, Thomas M. (Sterling-Winthrop Research Institute, Rensselaer, N.Y.), William H. Deitz, and William A. Goss. Mechanism of action of nalidixic acid on Escherichia coli. IV. Effects on the stability of cellular constituents. J. Bacteriol. 91:774-779. 1996.-Treatment of Escherichia coli 15TAU with nalidixic acid resu...

journal_title：Journal of bacteriology

authors： Cook TM,Deitz WH,Goss WA

更新日期：1966-02-01 00:00:00

abstract：:Pseudomonas fluorescens strain KU-7 is a prototype microorganism that metabolizes 2-nitrobenzoate (2-NBA) via the formation of 3-hydroxyanthranilate (3-HAA), a known antioxidant and reductant. The initial two steps leading to the sequential formation of 2-hydroxy/aminobenzoate and 3-HAA are catalyzed by a NADPH-depend...

journal_title：Journal of bacteriology

authors： Iwaki H,Muraki T,Ishihara S,Hasegawa Y,Rankin KN,Sulea T,Boyd J,Lau PC

更新日期：2007-05-01 00:00:00

abstract：:Bacillus subtilis 168ts-200B is a temperature-sensitive mutant of B. subtilis 168 which grows as rods at 30 C but as irregular spheres at 45 C. Growth at the nonpermissive temperature resulted in a deficiency of teichoic acid in the cell wall. A decrease in teichoic acid synthesis coupled with the rapid turnover of th...

journal_title：Journal of bacteriology

authors： Boylan RJ,Mendelson NH,Brooks D,Young FE

更新日期：1972-04-01 00:00:00

abstract：:The distribution of the extracellular enzyme, thiaminase I, was determined for logarithmically growing cultures of Bacillus thiaminolyticus. About 60% of the enzyme is associated with the cells throughout the growth cycle. The remainder of the enzyme is in the culture medium. The release of the cell-bound thiaminase I...

journal_title：Journal of bacteriology

authors： Agee CC,Wilkins JH,Airth RL

更新日期：1973-09-01 00:00:00

abstract：:Several phosphate-starvation-inducible (psi) genetic loci in Salmonella typhimurium were identified by fusing the lacZ gene to psi promoters by using the Mu d1 and Mu d1-8 bacteriophages. Although several different starvation conditions were examined, the psi loci responded solely to phosphate deprivation. A regulator...

journal_title：Journal of bacteriology

authors： Foster JW,Spector MP

更新日期：1986-05-01 00:00:00

abstract：:Tn2603 is a multiple-resistance transposon encoding resistance to ampicillin, streptomycin, sulfonamide, and mercury and having a molecular size of 20 kilobase pairs, with 200-base-pair inverted repeats at both ends. The essential sites and functions of Tn2603 which are required for its transposition were determined t...

journal_title：Journal of bacteriology

authors： Tanaka M,Harafuji H,Yamamoto T

更新日期：1982-08-01 00:00:00

abstract：:Alicyclobacillus acidocaldarius strain Tc-4-1 was initially isolated from a hot spring in Tengchong, China. This organism is both thermophilic and acidophilic. It can produce heat- and acid-stable enzymes, such as amylase and esterase, which may be important in industry. Here we report the whole genome sequence of the...

journal_title：Journal of bacteriology

authors： Chen Y,He Y,Zhang B,Yang J,Li W,Dong Z,Hu S

更新日期：2011-10-01 00:00:00

abstract：:Ultrastructural and biochemical studies of a recombinant Treponema pallidum surface antigen designated 4D have been conducted due to its likely biological significance. Electron microscopy demonstrated that the 190-kilodalton (kDa) 4D molecule is an ordered ring structure of 10-nm diameter. The 90-kDa proteinase K-tre...

journal_title：Journal of bacteriology

authors： Fehniger TE,Radolf JD,Lovett MA

更新日期：1986-03-01 00:00:00