Molecular and functional characterization of a unique sucrose hydrolase from Xanthomonas axonopodis pv. glycines.

abstract：:The fungal pathogen Cryptococcus neoformans is killed by the bacterium Staphylococcus aureus, and the killing is inhibited by soluble capsular polysaccharides. To investigate the mechanism of killing, cells in coculture were examined by scanning and transmission electron microscopy. S. aureus attached to the capsule o...

journal_title：Journal of bacteriology

authors： Ikeda R,Saito F,Matsuo M,Kurokawa K,Sekimizu K,Yamaguchi M,Kawamoto S

更新日期：2007-07-01 00:00:00

abstract：:Cells of Ureaplasma urealyticum that were prepared by a ruthenium red technique demonstrated an extramembranous layer of polyanions of about 15 to 30 nm in width. Application of the concanavalin A-iron dextran strain indicated that the outer surface of this layer contained glucosyl-like residues. ...

journal_title：Journal of bacteriology

authors： Robertson J,Smook E

更新日期：1976-11-01 00:00:00

abstract：:Analysis of chlorobenzene-degrading transconjugants of Pseudomonas putida F1 which had acquired the genes for chlorocatechol degradation (clc) from Pseudomonas sp. strain B13 revealed that the clc gene cluster was present on a 105-kb amplifiable genetic element (named the clc element). In one such transconjugant, P. p...

journal_title：Journal of bacteriology

authors： Ravatn R,Studer S,Springael D,Zehnder AJ,van der Meer JR

更新日期：1998-09-01 00:00:00

abstract：:Two novel insertion sequences, IS1312 and IS1313, were found in pTiBo542, the Ti plasmid of Agrobacterium tumefaciens strains Bo542 and A281. Nucleotide sequencing and Southern hybridization revealed that IS1312 and IS1313 are homologous to Rhizobium meliloti ISRm1 and ISRm2, respectively. IS1312, ISRm1, and another A...

journal_title：Journal of bacteriology

authors： Deng W,Gordon MP,Nester EW

更新日期：1995-05-01 00:00:00

abstract：:Penicillin-binding proteins were identified in Borrelia burgdorferi membranes. A 94-kilodalton penicillin-binding protein was the first to be labeled with tritiated penicillin and was the first band to disappear in a competition experiment. Its binding ability was destroyed when membranes were preboiled. In addition, ...

journal_title：Journal of bacteriology

authors： Urban C,Rahal JJ,Dattwyller RJ,Gorevic P,Luft BJ

更新日期：1990-10-01 00:00:00

abstract：:A thermosensitive episome bearing the transposon Tn10, F(Ts)::Tn10 Lac+, has been successfully transferred from Escherichia coli to several wild strains of the enterobacteria Erwinia carotovora subsp. chrysanthemi, which are pathogenic on Saintpaulia ionantha. In one of these strains, all of the characters controlled ...

journal_title：Journal of bacteriology

authors： Kotoujansky A,Lemattre M,Boistard P

更新日期：1982-04-01 00:00:00

abstract：:Upon induction, Bacillus megaterium 216 produces the bacteriocin megacin A-216, which leads to lysis of the producer cell and kills B. megaterium and a few other bacterial species. The DNA region responsible for megacinogeny was cloned in B. megaterium. The nucleotide sequence of a 5,494-bp-long subfragment was determ...

journal_title：Journal of bacteriology

authors： Kiss A,Balikó G,Csorba A,Chuluunbaatar T,Medzihradszky KF,Alföldi L

更新日期：2008-10-01 00:00:00

abstract：:Although arsenic is highly toxic to most organisms, certain prokaryotes are known to grow on and respire toxic metalloids of arsenic (i.e., arsenate and arsenite). Two enzymes are known to be required for this arsenic-based metabolism: (i) the arsenate respiratory reductase (ArrA) and (ii) arsenite oxidase (AoxB). Bot...

journal_title：Journal of bacteriology

authors： Zargar K,Hoeft S,Oremland R,Saltikov CW

更新日期：2010-07-01 00:00:00

abstract：:Morello, Josephine A. (Boston University School of Medicine, Boston, Mass.), Teresa A. DiGenio, and Edgar E. Baker. Significance of salmonellae isolated from apparently healthy mice. J. Bacteriol. 89:1460-1464. 1965.-Three species of Salmonella were isolated from three groups of apparently healthy laboratory mice. The...

journal_title：Journal of bacteriology

authors： MORELLO JA,DIGENIO TA,BAKER EE

更新日期：1965-06-01 00:00:00

abstract：:Most bacteriophages (phages) release their progeny through the action of holins that form lesions in the cytoplasmic membrane and lysins that degrade the bacterial peptidoglycan. Although the function of each protein is well established in phages infecting Streptococcus pneumoniae, the role--if any--of the powerful ba...

journal_title：Journal of bacteriology

authors： Frias MJ,Melo-Cristino J,Ramirez M

更新日期：2009-09-01 00:00:00

abstract：:The conjugally acquired deoxyribonucleic acid (DNA) of small, anucleate cells ("minicells") of a mutant strain of Escherichia coli K-12 was found to be predominantly associated with the bacterial membrane. Evidence from X-irradiation studies in vivo shows that there is no decrease in DNA-membrane association under con...

journal_title：Journal of bacteriology

authors： Shull FW Jr,Fralick JA,Stratton LP,Fisher WD

更新日期：1971-05-01 00:00:00

abstract：:Atomic force microscopy was used to measure the thickness of air-dried, collapsed murein sacculi from Escherichia coli K-12 and Pseudomonas aeruginosa PAO1. Air-dried sacculi from E. coli had a thickness of 3.0 nm, whereas those from P. aeruginosa were 1.5 nm thick. When rehydrated, the sacculi of both bacteria swelle...

journal_title：Journal of bacteriology

authors： Yao X,Jericho M,Pink D,Beveridge T

更新日期：1999-11-01 00:00:00

abstract：:The quorum-sensing system of Staphylococcus aureus, the accessory gene regulator (Agr) system, is responsible for increased transcription of certain exoprotein genes and decreased transcription of certain cell wall-associated proteins during the postexponential phase of growth. This regulation is important for virulen...

journal_title：Journal of bacteriology

authors： Tseng CW,Zhang S,Stewart GC

更新日期：2004-03-01 00:00:00

abstract：:Growth of Escherichia coli in the presence of certain D-amino acids, such as D-methionine, results in the incorporation of the D-amino acid into macromolecular peptidoglycan and can be lethal at high concentrations. Previous studies suggested that incorporation was independent of the normal biosynthetic pathway. An en...

journal_title：Journal of bacteriology

authors： Caparrós M,Pisabarro AG,de Pedro MA

更新日期：1992-09-01 00:00:00

abstract：:The papA gene of the uropathogenic strain Escherichia coli J96, coding for the Pap pili subunit, was subjected to DNA sequencing, and found to code for an 185-amino acid-long polypeptide with a 22-amino acid-long signal peptide. Here we present the primary sequence, the hydrophilicity profile, and the predicted polype...

journal_title：Journal of bacteriology

authors： Båga M,Normark S,Hardy J,O'Hanley P,Lark D,Olsson O,Schoolnik G,Falkow S

更新日期：1984-01-01 00:00:00

abstract：:The exopolysaccharide of Pseudomonas marginalis HT041B has been characterized as a 1,3-linked galactoglucan in which galactose and glucose are in the alpha- and beta-anomeric configurations, respectively. The polysaccharide is substituted with pyruvate at the 4 and 6 positions of galactose and with succinic acid at ei...

journal_title：Journal of bacteriology

authors： Osman SF,Fett WF

更新日期：1989-03-01 00:00:00

abstract：:Bacillus brevis 47 contains two cell wall proteins termed the outer wall protein (OWP) and middle wall protein (MWP), each of which forms hexagonal arrays in the cell wall. A 6-kilobase BglII-BclI fragment of B. brevis 47 DNA cloned into Bacillus subtilis with a derivative of pHW1 as a vector directed the synthesis of...

journal_title：Journal of bacteriology

authors： Tsuboi A,Uchihi R,Tabata R,Takahashi Y,Hashiba H,Sasaki T,Yamagata H,Tsukagoshi N,Udaka S

更新日期：1986-10-01 00:00:00

abstract：:Four genes that encode the homologues of plant geranylgeranyl reductase were isolated from a hyperthermophilic archaeon Archaeoglobus fulgidus, which produces menaquinone with a fully saturated heptaprenyl side chain, menaquinone-7(14H). The recombinant expression of one of the homologues in Escherichia coli led to a ...

journal_title：Journal of bacteriology

authors： Hemmi H,Takahashi Y,Shibuya K,Nakayama T,Nishino T

更新日期：2005-03-01 00:00:00

abstract：:Mutants of Agrobacterium tumefaciens requiring methionine for growth on a solid basal medium were induced by the use of N-methyl-N'-nitro-N-nitrosoguanidine. In addition to the difference of mutant strains, the extent of methionine dependency differed in a liquid basal medium and in the presence of aspartate or fumara...

journal_title：Journal of bacteriology

authors： Kaneshiro T

更新日期：1968-06-01 00:00:00

abstract：:Bacteria able to use at least one of 13 ionic alkylphosphonates of O-alkyl or O,O-dialkyl alkylphosphonates as phosphorus sources were isolated from sewage and soil. Four of these isolates used 2-aminoethylphosphonic acid (AEP) as a sole carbon, nitrogen, and phosphorus source. None of the other phosphonates served as...

journal_title：Journal of bacteriology

authors： Cook AM,Daughton CG,Alexander M

更新日期：1978-01-01 00:00:00

abstract：:Using a sensitive primer extension technique, we have carried out studies to localize the start site of replication of the replicon RepFIC. In the course of these studies, we have found evidence that supports the hypothesis that transcription is an integral component of the initiation of replication. On the basis of o...

journal_title：Journal of bacteriology

authors： Maas R,Wang C

更新日期：1997-04-01 00:00:00

abstract：:Partially purified inactive glucose dehydrogenase obtained from spores which were heated at 87 or 90 C for 30 min is converted to an active from by the addition of ethylenediaminetetraacetic acid, dipicolinic acid, or some salts. The molecular weight of the inactive glucose dehydrogenase in the heated spores is about ...

journal_title：Journal of bacteriology

authors： Hachisuka Y,Tochikubo K

更新日期：1971-08-01 00:00:00

abstract：:The first enzymes of the histidine (hut) and proline degradative pathways, histidase and proline oxidase, could not be induced in Bacillus subtilis cells growing in glucose minimal medium containing a mixture of 16 amino acids. Addition of the 16-amino-acid mixture to induced wild-type cells growing in citrate minimal...

journal_title：Journal of bacteriology

authors： Atkinson MR,Wray LV Jr,Fisher SH

更新日期：1990-09-01 00:00:00

abstract：:The structure of sheathed flagella from Bdellovibrio bacteriovorus was investigated. The first three periods of these flagella were characterized by progressively smaller wavelengths and amplitudes in periods more distal to the cell. The damped appearance was due to a single nonrandom transition between two helical st...

journal_title：Journal of bacteriology

authors： Thomashow LS,Rittenberg SC

更新日期：1985-09-01 00:00:00

abstract：:The location and extent of the homology between plasmids ColE1 and p15A were determined by analysis of heteroduplexes formed between them as well as with a related plasmid, pBR322, and by hybridization of radioactive deoxyribonucleic acids to restriction fragments of p15A and ColE1. The homology between the plasmids c...

journal_title：Journal of bacteriology

authors： Bird RE

更新日期：1981-03-01 00:00:00

abstract：:The opportunistic pathogen Pseudomonas aeruginosa produces multifunctional, polar, filamentous appendages termed type IV pili. Type IV pili are involved in colonization during infection, twitching motility, biofilm formation, bacteriophage infection, and natural transformation. Electrostatic surface analysis of modele...

journal_title：Journal of bacteriology

authors： van Schaik EJ,Giltner CL,Audette GF,Keizer DW,Bautista DL,Slupsky CM,Sykes BD,Irvin RT

更新日期：2005-02-01 00:00:00

abstract：:Uninduced cultures of Saccharomyces cerevisiae exhibit high basal levels of allantoinase, allantoicase, and ureidoglycolate hydrolase, the enzymes responsible for degrading allantoin to urea. As a result, these activities increase only 4- to 8-fold upon induction, whereas the urea-degrading enzymes, urea carboxylase a...

journal_title：Journal of bacteriology

authors： Zacharski CA,Cooper TG

更新日期：1978-08-01 00:00:00

abstract：:In Escherichia coli K-12, the rcsA and rcsB gene products are positive regulators in expression of the slime polysaccharide colanic acid. We have previously demonstrated the presence of rcsA sequences in E. coli K1 and K5, strains with group II capsular K antigens, and shown that introduction of multicopy rcsA into th...

journal_title：Journal of bacteriology

authors： Keenleyside WJ,Bronner D,Jann K,Jann B,Whitfield C

更新日期：1993-10-01 00:00:00

abstract：:Specific localization of the lysis (L) protein of bacteriophage MS2 in the cell wall of Escherichia coli was determined by immunoelectron microscopy. After induction of the cloned lysis gene, the cells were plasmolyzed, fixed, and embedded in either Epon or Lowicryl K4M. Polyclonal L-protein-specific antiserum was pur...

journal_title：Journal of bacteriology

authors： Walderich B,Höltje JV

更新日期：1989-06-01 00:00:00

abstract：:A method consisting of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and subsequent detection of endoglucanases by blotting with a polyclonal antibody against endoglucanase I was used to investigate the effect of induction and carbon catabolite derepression on the synthesis of multiple forms of endoglucana...

journal_title：Journal of bacteriology

authors： Messner R,Gruber F,Kubicek CP

更新日期：1988-08-01 00:00:00