Chromosomal integration, tandem amplification, and deamplification in Pseudomonas putida F1 of a 105-kilobase genetic element containing the chlorocatechol degradative genes from Pseudomonas sp. Strain B13.

abstract：:Hydroxylamine mutagenesis was used to alter the tar gene that encodes the transmembrane Tar protein required for chemotaxis. Mutants defective in chemotaxis were selected, and the mutation was characterized by DNA sequencing. Two classes of mutations were found: nonsense and missense. The nonsense mutations were distr...

journal_title：Journal of bacteriology

authors： Mutoh N,Oosawa K,Simon MI

更新日期：1986-09-01 00:00:00

abstract：:Succinate dehydrogenase (EC 1.3.99.1) of Micrococcus luteus was selectively precipitated from Triton X-100-solubilized membranes by using specific antiserum. The precipitated enzyme contained equimolar amounts of four polypeptides with apparent molecular weights of 72,000, 30,000, 17,000, and 15,000. The 72,000 polype...

journal_title：Journal of bacteriology

authors： Crowe BA,Owen P

更新日期：1983-03-01 00:00:00

abstract：:The genetic context, phylogeny, and biochemistry of a gene flanking the H(2)-forming methylene-H(4)-methanopterin dehydrogenase gene (hmdA), here designated hmdB, indicate that it is a new member of the radical S-adenosylmethionine enzyme superfamily. In contrast to the characteristic CX(3)CX(2)C or CX(2)CX(4)C motif ...

journal_title：Journal of bacteriology

authors： McGlynn SE,Boyd ES,Shepard EM,Lange RK,Gerlach R,Broderick JB,Peters JW

更新日期：2010-01-01 00:00:00

abstract：:Recently we identified a bacterial factor (BimA) required for actin-based motility of Burkholderia pseudomallei. Here we report that Burkholderia mallei and Burkholderia thailandensis are capable of actin-based motility in J774.2 cells and that BimA homologs of these bacteria can restore the actin-based motility defec...

journal_title：Journal of bacteriology

authors： Stevens JM,Ulrich RL,Taylor LA,Wood MW,Deshazer D,Stevens MP,Galyov EE

更新日期：2005-11-01 00:00:00

abstract：:The activity of beta-glucosidase (EC 3.2.1.21) in extracts of Dictyostelium discoideum was investigated. The specific activity increased early in development, declined during pseudoplasmodium formation, and increased again during sorocarp formation. The beta-glucosidase which was present in growing amoebae and during ...

journal_title：Journal of bacteriology

authors： Coston MB,Loomis WF Jr

更新日期：1969-12-01 00:00:00

abstract：:XbaI digestion and pulsed-field gel electrophoresis of the genome of Salmonella typhimurium LT2 yields 24 fragments: 23 fragments (total size, 4,807 kb) are from the chromosome, and one fragment (90 kb) is from the virulence plasmid pSLT. Some of the 23 fragments from the chromosome were located on the linkage map by ...

journal_title：Journal of bacteriology

authors： Liu SL,Sanderson KE

更新日期：1992-03-01 00:00:00

abstract：:Escherichia coli lactose permease mediates the proton-driven translocation of galactosides across the cytoplasmic membrane. To define regions important for membrane insertion as well as for biological function, we constructed plasmids encoding different portions of the lactose carrier. Among several lacY deletions, tw...

journal_title：Journal of bacteriology

authors： Wrubel W,Stochaj U,Sonnewald U,Theres C,Ehring R

更新日期：1990-09-01 00:00:00

abstract：:Borrelia burgdorferi, the Lyme disease agent, and other members of the spirochetal genus Borrelia have double-stranded linear plasmids in addition to supercoiled circular plasmids. The copy number relative to the chromosome was determined for 49- and 16-kb linear plasmids and a 27-kb circular plasmid of the type strai...

journal_title：Journal of bacteriology

authors： Hinnebusch J,Barbour AG

更新日期：1992-08-01 00:00:00

abstract：:A Pediococcus cerevisiae mutant that actively accumulated folate (PteGlu), in contrast to the wild-type, was also found to exhibit changes in the pattern of uptake of 5-methyl-tetrahydrofolate (5-CH3-H4PteGlu) and amethopterin. Most of the 5-CH3-H4PteGlue accumulated through a glucose- and temperature-dependent proces...

journal_title：Journal of bacteriology

authors： Mandelbaum-Shavit F,Grossowicz N

更新日期：1975-08-01 00:00:00

abstract：:In Escherichia coli, the CpxRA two-component signal transduction system senses and responds to aggregated and misfolded proteins in the bacterial envelope. We show that CpxR-P (the phosphorylated form of the cognate response regulator) activates cpxRA expression in conjunction with RpoS, suggesting an involvement of t...

journal_title：Journal of bacteriology

authors： De Wulf P,Kwon O,Lin EC

更新日期：1999-11-01 00:00:00

abstract：:The two-component regulatory system, OmpR-EnvZ, of Escherichia coli K-12 regulates the expression of the major outer membrane porin protein, OmpF. OmpR is a DNA-binding protein which acts as both an activator and a repressor to control ompF transcription. In this article, we describe a new OmpR-binding site that is lo...

journal_title：Journal of bacteriology

authors： Huang KJ,Schieberl JL,Igo MM

更新日期：1994-03-01 00:00:00

abstract：:The human bacterial pathogens Chlamydia spp. possess a genus-specific lipopolysaccharide as a major surface antigen, the structure of which has been determined by analytical chemistry as Kdop alpha 2-8-Kdop alpha 2-4-Kdop alpha 2-6GlcNp beta 1-6-GlcNol (Kdo, 3-deoxy-D-manno-2-octulosonic acid). Immunochemical studies ...

journal_title：Journal of bacteriology

authors： Holst O,Brade L,Kosma P,Brade H

更新日期：1991-03-01 00:00:00

abstract：:The chb operon of Escherichia coli is involved in the utilization of the β-glucosides chitobiose and cellobiose. The function of chbG (ydjC), the sixth open reading frame of the operon that codes for an evolutionarily conserved protein is unknown. We show that chbG encodes a monodeacetylase that is essential for growt...

journal_title：Journal of bacteriology

authors： Verma SC,Mahadevan S

更新日期：2012-09-01 00:00:00

abstract：:Moorella thermoacetica can grow with H₂ and CO₂, forming acetic acid from 2 CO₂ via the Wood-Ljungdahl pathway. All enzymes involved in this pathway have been characterized to date, except for methylenetetrahydrofolate reductase (MetF). We report here that the M. thermoacetica gene that putatively encodes this enzyme,...

journal_title：Journal of bacteriology

authors： Mock J,Wang S,Huang H,Kahnt J,Thauer RK

更新日期：2014-09-01 00:00:00

abstract：:The enzyme nicotinamide mononucleotide (NMN) adenylyltransferase (EC 2.7.7.1) catalyzes the synthesis of NAD+ and nicotinic acid adenine dinucleotide. It has been purified to homogeneity from cellular extracts of the thermophilic archaeon Sulfolobus solfataricus. Through a database search, a highly significant match w...

journal_title：Journal of bacteriology

authors： Raffaelli N,Pisani FM,Lorenzi T,Emanuelli M,Amici A,Ruggieri S,Magni G

更新日期：1997-12-01 00:00:00

abstract：:Growth of Lactococcus lactis in milk depends on the utilization of extracellular peptides. Up to now, oligopeptide uptake was thought to be due only to the ABC transporter Opp. Nevertheless, analysis of several Opp-deficient L. lactis strains revealed the implication of a second oligopeptide ABC transporter, the so-ca...

journal_title：Journal of bacteriology

authors： Lamarque M,Charbonnel P,Aubel D,Piard JC,Atlan D,Juillard V

更新日期：2004-10-01 00:00:00

abstract：:A primary alcohol dehydrogenase has been purified from Methylococcus capsulatus (Texas strain). The purified enzyme catalyzes the oxidation of methanol and formaldehyde to formate; other primary alcohols are oxidized to their corresponding aldehydes. Ammonium ions are required for enzyme activity. The enzyme has a mol...

journal_title：Journal of bacteriology

authors： Patel RN,Bose HR,Mandy WJ,Hoare DS

更新日期：1972-05-01 00:00:00

abstract：:Carbon catabolite repression (CCR) allows bacteria to alter metabolism in response to the availability of specific sugar sources, and increasing evidence suggests that CCR is involved in regulating virulence gene expression in many pathogens. A scan of the M1 SF370 group A streptococcus (GAS) genome using a Bacillus s...

journal_title：Journal of bacteriology

authors： Almengor AC,Kinkel TL,Day SJ,McIver KS

更新日期：2007-12-01 00:00:00

abstract：:The mutant sigA allele of Bacillus subtilis DB1005 was confirmed to be temperature sensitive (ts) and transferable among strains of B. subtilis by chromosomal transformation and gene conversion. This ts sigA allele had a pleiotropic effect on gene expression of DB1005. The induction of certain heat shock proteins in D...

journal_title：Journal of bacteriology

authors： Chang BY,Chen KY,Wen YD,Liao CT

更新日期：1994-06-01 00:00:00

abstract：:The endophytic diazotroph Azoarcus sp. strain BH72 is capable of infecting rice roots and of expressing the nitrogenase (nif) genes there. In order to study the genetic background for nitrogen fixation in strain BH72, the structural genes of nitrogenase (nifHDK) were cloned and sequenced. The sequence analysis reveale...

journal_title：Journal of bacteriology

authors： Egener T,Martin DE,Sarkar A,Reinhold-Hurek B

更新日期：2001-06-01 00:00:00

abstract：:The endophytic diazotroph Gluconacetobacter diazotrophicus secretes a constitutively expressed levansucrase (LsdA, EC 2.4.1.10) to utilize plant sucrose. LsdA, unlike other extracellular levansucrases from gram-negative bacteria, is transported to the periplasm by a signal-peptide-dependent pathway. We identified an u...

journal_title：Journal of bacteriology

authors： Arrieta JG,Sotolongo M,Menéndez C,Alfonso D,Trujillo LE,Soto M,Ramírez R,Hernández L

更新日期：2004-08-01 00:00:00

abstract：:To examine as randomly as possible the role of the beta-ketoacyl and acyl carrier protein (ACP) components of bacterial type II polyketide synthases (PKSs), homologs of the chain-length-factor (CLF) genes were cloned from the environmental community of microorganisms. With PCR primers derived from conserved regions of...

journal_title：Journal of bacteriology

authors： Seow KT,Meurer G,Gerlitz M,Wendt-Pienkowski E,Hutchinson CR,Davies J

更新日期：1997-12-01 00:00:00

abstract：:Listeria monocytogenes is able to efficiently utilize glycerol as a carbon source. In a defined minimal medium, the growth rate (during balanced growth) in the presence of glycerol is similar to that in the presence of glucose or cellobiose. Comparative transcriptome analyses of L. monocytogenes showed high-level tran...

journal_title：Journal of bacteriology

authors： Joseph B,Mertins S,Stoll R,Schär J,Umesha KR,Luo Q,Müller-Altrock S,Goebel W

更新日期：2008-08-01 00:00:00

abstract：:Englesberg, E. (University of Pittsburgh, Pittsburgh, Pa.), R L. Anderson, R. Weinberg, N. Lee, P. Hoffee, G. Huttenhauer, and H. Boyer. l-Arabinose-sensitive, l-ribulose 5-phosphate 4-epimerase-deficient mutants of Escherichia coli. J. Bacteriol. 84:137-146. 1962-l-Arabinose-negative mutants of Escherichia coli B/r, ...

journal_title：Journal of bacteriology

authors： ENGLESBERG E,ANDERSON RL,WEINBERG R,LEE N,HOFFEE P,HUTTENHAUER G,BOYER H

更新日期：1962-07-01 00:00:00

abstract：:Wild-type Escherichia coli cells are unable to grow on beta-glucosides. Spontaneous mutants arise, however, which are able to utilize certain aromatic beta-glucosides such as salicin or arbutin as carbon sources, revealing the presence of a cryptic operon called bgl. Mutations activating the operon map within (or clos...

journal_title：Journal of bacteriology

authors： Schnetz K,Toloczyki C,Rak B

更新日期：1987-06-01 00:00:00

abstract：:Reich, Paul R. (National Institutes of Health, Bethesda, Md.), Norman L. Somerson, James A. Rose, and Sherman M. Weissman. Genetic relatedness among mycoplasmas as determined by nucleic acid homology. J. Bacteriol. 91:153-160. 1966.-A sensitive membrane filter method to detect nucleic acid homology was used to determi...

journal_title：Journal of bacteriology

authors： Reich PR,Somerson NL,Rose JA,Weissman SM

更新日期：1966-01-01 00:00:00

abstract：:The mutB gene of Salmonella typhimurium is involved in a methylation-independent repair pathway specific for A/G or A/C mismatches and is the homolog of the Escherichia coli mutY gene. The mutB gene of S. typhimurium was cloned and sequenced. The isolated mutB clone reduced the mutation rate of the mutB mutant to wild...

journal_title：Journal of bacteriology

authors： Desiraju V,Shanabruch WG,Lu AL

更新日期：1993-01-01 00:00:00

abstract：:The 2,647-bp nucleotide sequence of cryptic plasmid pTD1, isolated from the oral spirochete Treponema denticola, was determined. The sequence revealed two open reading frames, A and B, which encode polypeptides of 335 and 235 amino acids, respectively. Open reading frame A shows sequence similarity to genes that encod...

journal_title：Journal of bacteriology

authors： MacDougall J,Margarita D,Saint Girons I

更新日期：1992-04-01 00:00:00

abstract：:The Arctic plant growth-promoting rhizobacterium Pseudomonas putida GR12-2 secretes an antifreeze protein (AFP) that promotes survival at subzero temperatures. The AFP is unusual in that it also exhibits a low level of ice nucleation activity. A DNA fragment with an open reading frame encoding 473 amino acids was clon...

journal_title：Journal of bacteriology

authors： Muryoi N,Sato M,Kaneko S,Kawahara H,Obata H,Yaish MW,Griffith M,Glick BR

更新日期：2004-09-01 00:00:00

abstract：:Among strains of Haemophilus influenzae, the ability to catabolize tryptophan (as detected by indole production) varies and is correlated with pathogenicity. Tryptophan catabolism is widespread (70 to 75%) among harmless respiratory isolates but is nearly universal (94 to 100%) among strains causing serious disease, i...

journal_title：Journal of bacteriology

authors： Martin K,Morlin G,Smith A,Nordyke A,Eisenstark A,Golomb M

更新日期：1998-01-01 00:00:00