A distant upstream site involved in the negative regulation of the Escherichia coli ompF gene.

Abstract:

:The two-component regulatory system, OmpR-EnvZ, of Escherichia coli K-12 regulates the expression of the major outer membrane porin protein, OmpF. OmpR is a DNA-binding protein which acts as both an activator and a repressor to control ompF transcription. In this article, we describe a new OmpR-binding site that is located between 384 to 351 bp upstream from the ompF start point of transcription. Inactivation of this site by insertion of a 22-bp fragment prevents the repression of ompF expression conferred by the dominant negative mutation, envZ473. On the basis of the location of this binding site, the presence of bent DNA in the ompF regulatory region (T. Mizuno, Gene 54:57-64, 1987), and the fact that mutations altering integration host factor result in constitutive ompF expression (P. Tsui, V. Helu, and M. Freundlich, J. Bacteriol. 170:4950-4953, 1988), we propose that the negative regulation of ompF involves a DNA loop structure.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Huang KJ,Schieberl JL,Igo MM

doi

10.1128/jb.176.5.1309-1315.1994

subject

Has Abstract

pub_date

1994-03-01 00:00:00

pages

1309-15

issue

5

eissn

0021-9193

issn

1098-5530

journal_volume

176

pub_type

杂志文章
  • Effects of m-Tyrosine on Growth and Sporulation of Bacillus Species.

    abstract::Aronson, John N. (Arizona State University, Tempe), and Gerald R. Wermus. Effects of m-tyrosine on growth and sporulation of Bacillus species. J. Bacteriol. 90:38-46. 1965.-The aromatic amino acid analogue, dl-beta-(3-hydroxyphenyl)alanine (m-tyrosine), reduced sporulation of a strain of Bacillus subtilis to less than...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.90.1.38-46.1965

    authors: Aronson JN,Wermus GR

    更新日期:1965-07-01 00:00:00

  • Multicellular stalk-like structures in Saccharomyces cerevisiae.

    abstract::Stalk formation is a novel pattern of multicellular organization. Yeast cells which survive UV irradiation form colonies that grow vertically to form very long (0.5 to 3.0 cm) and thin (0.5 to 4 mm in diameter) multicellular structures. We describe the conditions required to obtain these stalk-like structures reproduc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.15.3992-3996.1998

    authors: Engelberg D,Mimran A,Martinetto H,Otto J,Simchen G,Karin M,Fink GR

    更新日期:1998-08-01 00:00:00

  • Involvement of gamma-glutamyl peptides in osmoadaptation of Escherichia coli.

    abstract::Accumulation of K+ ions and glutamate plays a primary role in maintaining osmotic balance in Escherichia coli, as illustrated by the high concentrations of these ions present in cells growing in medium of high osmolality. We found that two gamma-glutamyl peptides and glutamine also accumulated during growth at high os...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.7.3631-3636.1990

    authors: McLaggan D,Logan TM,Lynn DG,Epstein W

    更新日期:1990-07-01 00:00:00

  • Beta-2-linked glucans secreted by fast-growing species of Rhizobium.

    abstract::Fast-growing species of Rhizobium were found to secrete low-molecular-weight beta-2-linked glucans when cultured in synthetic liquid medium. These glucans are quite similar to beta-2-linked glucans produced by species of Agrobacterium. No reducing terminus was detected in these glucans. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.142.1.243-248.1980

    authors: York WS,McNeil M,Darvill AG,Albersheim P

    更新日期:1980-04-01 00:00:00

  • An O-antigen processing function for Wzx (RfbX): a promising candidate for O-unit flippase.

    abstract::O antigen is the major cell surface antigen of gram-negative bacteria, and the genes responsible for its synthesis are located in a single gene cluster. The wzx (rbfX) gene, which is characteristic of the major class of O-antigen gene clusters, encodes a hydrophobic protein with 12 potential transmembrane segments. We...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.7.2102-2107.1996

    authors: Liu D,Cole RA,Reeves PR

    更新日期:1996-04-01 00:00:00

  • Streptococcus pyogenes type 12 M protein gene regulation by upstream sequences.

    abstract::A partial nucleotide sequence that included 1,693 base pairs of the M12 (emm12) gene of group A streptococci (strain CS24) and adjacent upstream DNA was determined. Type 12 M protein-specific mRNA of strain CS24 is transcribed from two promoters (P1 and P3) separated by 30 bases. The transcription start sites of the e...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.12.5633-5640.1987

    authors: Robbins JC,Spanier JG,Jones SJ,Simpson WJ,Cleary PP

    更新日期:1987-12-01 00:00:00

  • Purification and N-terminal sequence of the alpha subunit of antigen 43, a unique protein complex associated with the outer membrane of Escherichia coli.

    abstract::Antigen 43 has been identified as a unique protein complex in the outer membrane of Escherichia coli. The complex contains two different polypeptides, alpha (Mr, 60,000) and beta (Mr, 53,000), in equal stoichiometry (P. Owen, P. Caffrey, and L.-G. Josefsson, J. Bacteriol. 169:3770-3777, 1987). The alpha subunit was re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.7.3634-3640.1989

    authors: Caffrey P,Owen P

    更新日期:1989-07-01 00:00:00

  • Functionally essential interaction between Yersinia YscO and the T3S4 domain of YscP.

    abstract::The type III secretion (T3S) system is essential to the virulence of a large number of Gram-negative bacterial pathogens, including Yersinia. YscO is required for T3S in Yersinia and is known to interact with several other T3S proteins, including the chaperone SycD and the needle length regulator YscP. To define which...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00876-13

    authors: Mukerjea R,Ghosh P

    更新日期:2013-10-01 00:00:00

  • Genetic analysis of the glutamate permease in Escherichia coli K-12.

    abstract::The glutamate permeation system in Escherichia coli K-12 consists of three genes: gltC, gltS, and gltR. The genes gltC and gltS are very closely linked, and are located between the pyrE and tna loci, in the following order: tna, gltC, gltS, pyrE; gltR is located near the metA gene. The three glt genes constitute a reg...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.97.3.1118-1128.1969

    authors: Marcus M,Halpern YS

    更新日期:1969-03-01 00:00:00

  • Secretion of a heterologous protein from Bacillus subtilis with the aid of protease signal sequences.

    abstract::Secretion vectors based on the genes from Bacillus amyloliquefaciens P for alkaline protease (aprBamP) and neutral protease (nprBamP) were constructed. With both aprBamP and nprBamP, a unique restriction site was introduced 3' of the predicted signal coding region by using the technique of oligonucleotide-directed mut...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.165.3.837-842.1986

    authors: Vasantha N,Thompson LD

    更新日期:1986-03-01 00:00:00

  • Acid-base titration of streptococci and the physical states of intracellular ions.

    abstract::Acid titrations of intact and butanol-treated cells of Streptococcus faecalis revealed that nearly all of the intracellular K(+) ions could diffuse into the suspending medium in association with small anions, including ribonucleic acid breakdown products, when the cell membrane was damaged. In contrast, nearly all of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.114.2.491-498.1973

    authors: Marquis RE,Porterfield N,Matsumura P

    更新日期:1973-05-01 00:00:00

  • Identification of a specific membrane-particle-associated DNA sequence in Bacillus subtilis.

    abstract::After the Bacillus subtilis nucleoid was dissected with restriction endonucleases, a specific DNA sequence from the purA region was isolated in a particulate form that probably originated from the cell membrane. Precise definition of the binding region within this sequence was achieved by a novel procedure based on a ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.166.1.38-43.1986

    authors: Sargent MG,Bennett MF

    更新日期:1986-04-01 00:00:00

  • Functional identification of the product of the Bacillus subtilis yvaL gene as a SecG homologue.

    abstract::Protein export in Escherichia coli is mediated by translocase, a multisubunit membrane protein complex with SecA as the peripheral subunit and the SecY, SecE, and SecG proteins as the integral membrane domain. In the gram-positive bacterium Bacillus subtilis, SecA, SecY, and SecE have been identified through genetic a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.6.1786-1792.1999

    authors: van Wely KH,Swaving J,Broekhuizen CP,Rose M,Quax WJ,Driessen AJ

    更新日期:1999-03-01 00:00:00

  • Multiple sigma factor genes in Brevibacterium lactofermentum: characterization of sigA and sigB.

    abstract::Four rpoD hybridizing signals have been identified in the chromosome of Brevibacterium lactofermentum. Two rpoD-like genes, sigA and sigB, have been cloned and sequenced, and they encode principal sigma factors of the RNA polymerase. The deduced amino acid sequences of SigA and SigB showed very high similarities to th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.2.550-553.1996

    authors: Oguiza JA,Marcos AT,Malumbres M,Martín JF

    更新日期:1996-01-01 00:00:00

  • Regulation of the SOS response analyzed by RecA protein amplification.

    abstract::A split UV light dose procedure was used in Escherichia coli to induce an SOS function, RecA protein amplification, which was measured by an immunoradiometric assay. The SOS system was partially induced after the first UV irradiation, and the inducing effects of subsequent identical UV doses were quantified. Variation...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.3.1162-1165.1985

    authors: Calsou P,Salles B

    更新日期:1985-06-01 00:00:00

  • Mapping of genes involved in macromolecular synthesis on the chromosome of Streptomyces coelicolor A3(2).

    abstract::The genes for the beta, beta', and seven sigma factor subunits of RNA polymerase, for elongation factors EF-Tu1 and EF-Tu3, and for six rRNA operons were mapped on the combined genetic and physical map of the Streptomyces coelicolor chromosome. Like the previously mapped tRNA genes, the RNA polymerase and rRNA genes m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.2.473-476.1995

    authors: van Wezel GP,Buttner MJ,Vijgenboom E,Bosch L,Hopwood DA,Kieser HM

    更新日期:1995-01-01 00:00:00

  • Ultrastructal morphology of some prokaryotic microorganisms associated with the hindgut of cockroaches.

    abstract::Scanning electron microscopy and transmission electron microscopy have been used to visualize the morphology and ultrastructure of two types of microorganisms in the hindgut of the cockroach Blaberus posticus. Both organisms, designated as either short or long rods, are attached to chitinous projections from the gut w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.123.1.336-345.1975

    authors: Foglesong MA,Walker DH Jr,Puffer JS,Markovetz AJ

    更新日期:1975-07-01 00:00:00

  • Expanded metabolic reconstruction of Helicobacter pylori (iIT341 GSM/GPR): an in silico genome-scale characterization of single- and double-deletion mutants.

    abstract::Helicobacter pylori is a human gastric pathogen infecting almost half of the world population. Herein, we present an updated version of the metabolic reconstruction of H. pylori strain 26695 based on the revised genome annotation and new experimental data. This reconstruction, iIT341 GSM/GPR, represents a detailed rev...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.16.5818-5830.2005

    authors: Thiele I,Vo TD,Price ND,Palsson BØ

    更新日期:2005-08-01 00:00:00

  • Heterologous expression of Bartonella adhesin A in Escherichia coli by exchange of trimeric autotransporter adhesin domains results in enhanced adhesion properties and a pathogenic phenotype.

    abstract::Human-pathogenic Bartonella henselae causes cat scratch disease and vasculoproliferative disorders. An important pathogenicity factor of B. henselae is the trimeric autotransporter adhesin (TAA) Bartonella adhesin A (BadA), which is modularly constructed, consisting of a head, a long and repetitive neck-stalk module, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01461-13

    authors: Schmidgen T,Kaiser PO,Ballhorn W,Franz B,Göttig S,Linke D,Kempf VA

    更新日期:2014-06-01 00:00:00

  • cAMP and Vfr Control Exolysin Expression and Cytotoxicity of Pseudomonas aeruginosa Taxonomic Outliers.

    abstract::The two-partner secretion system ExlBA, expressed by strains of Pseudomonas aeruginosa belonging to the PA7 group, induces hemorrhage in lungs due to disruption of host cellular membranes. Here we demonstrate that the exlBA genes are controlled by a pathway consisting of cAMP and the virulence factor regulator (Vfr). ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00135-18

    authors: Berry A,Han K,Trouillon J,Robert-Genthon M,Ragno M,Lory S,Attrée I,Elsen S

    更新日期:2018-05-24 00:00:00

  • Identification and characterization of CAMP cohemolysin as a potential virulence factor of Riemerella anatipestifer.

    abstract::Riemerella anatipestifer is responsible for exudative septicemia in ducks. The genetic determinant of the CAMP cohemolysin, cam, from a strain of R. anatipestifer was cloned and expressed in Escherichia coli. Chromosomal DNA from serotype 19 strain 30/90 was used to construct a gene library in pBluescript II SK(-) vec...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.7.1932-1939.2002

    authors: Crasta KC,Chua KL,Subramaniam S,Frey J,Loh H,Tan HM

    更新日期:2002-04-01 00:00:00

  • pdc1(0) mutants of Saccharomyces cerevisiae give evidence for an additional structural PDC gene: cloning of PDC5, a gene homologous to PDC1.

    abstract::The PDC1 gene coding for a pyruvate decarboxylase (PDC; EC 4.1.1.1) was deleted from the Saccharomyces cerevisiae genome. The resulting pdc1(0) mutants were able to grow on glucose and still contained 60 to 70% of the wild-type PDC activity. Two DNA fragments with sequences homologous to that of the PDC1 gene were clo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.2.678-685.1990

    authors: Seeboth PG,Bohnsack K,Hollenberg CP

    更新日期:1990-02-01 00:00:00

  • Three-dimensional structure of the nickel-containing hydrogenase from Thiocapsa roseopersicina.

    abstract::The three-dimensional structure of the nickel-containing hydrogenase from Thiocapsa roseopersicina has been determined at a resolution of 2 nm in the plane and 4 nm in the vertical direction by electron microscopy and computerized image processing on microcrystals of the enzyme. The enzyme forms a large ring-shaped co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.8.2576-2580.1991

    authors: Sherman MB,Orlova EV,Smirnova EA,Hovmöller S,Zorin NA

    更新日期:1991-04-01 00:00:00

  • Electron micrography study of the asci and ascospores of Metschnikowia Kamienski.

    abstract::Internal and surface structures of asci and ascospores were studied by transmission electron microscopy (TEM) and by scanning electron microscopy (SEM) to establish the character and number of ascospores within the ascus of Metschnikowia krissii. Enzyme digestion with snail gut enzymes and SEM examination suggested th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.115.1.316-322.1973

    authors: Talens LT,Miller MW,Miranda M

    更新日期:1973-07-01 00:00:00

  • The cell envelope structure of the lipopolysaccharide-lacking gram-negative bacterium Sphingomonas paucimobilis.

    abstract::From the cell envelope preparation of Sphingomonas paucimobilis two membrane fractions with different densities were separated by sucrose density gradient ultracentrifugation. The high-density fraction contained several major proteins, phospholipids, and glycosphingolipids, which are the only glycolipids of this lipop...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.2.284-290.1994

    authors: Kawasaki S,Moriguchi R,Sekiya K,Nakai T,Ono E,Kume K,Kawahara K

    更新日期:1994-01-01 00:00:00

  • Sporulation in D-glucosamine auxotrophs of Saccharomyces cerevisiae: meiosis with defective ascospore wall formation.

    abstract::Mutants that require exogenous D-glucosamine for growth were isolated from Saccharomyces cerevisiae X2180-1A after ethyl methane sulfonate mutagenesis. Class A auxotrophs fail to grow on yeast extract-peptone-dextrose and minimal media, whereas class B auxotrophs grow on minimal medium and readily revert to grow on ye...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.124.3.1545-1557.1975

    authors: Whelan WL,Ballou CE

    更新日期:1975-12-01 00:00:00

  • Biogenesis of the fraction 1 capsule and analysis of the ultrastructure of Yersinia pestis.

    abstract::Analysis of a Yersinia pestis Delta caf1A mutant demonstrated that the Caf1A usher is required for the assembly and secretion of the fraction 1 capsule. The capsule assembled into thin fibrils and denser aggregates on the bacterial surface. Pilus-like fibers were also detected on the surface of Y. pestis. The capsule ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01840-07

    authors: Runco LM,Myrczek S,Bliska JB,Thanassi DG

    更新日期:2008-05-01 00:00:00

  • Interactions between exogenous deoxyribonucleic acid and membrane vesicles isolated from Bacillus subtilis 168.

    abstract::Membrane vesicles isolated from competent cultures of Bacillus subtilis 168 bound up to 20 mug of double-stranded deoxyribonucleic acid (DNA) per mg of membrane protein in the presence of ethylenediaminetetraacetate. The formation of the DNA-membrane complex was time, temperature, and pH dependent. Eighty per cent of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.119.3.784-794.1974

    authors: Joenje H,Konings WN,Venema G

    更新日期:1974-09-01 00:00:00

  • Purification and partial characterization of the principal deoxyribonucleic acid polymerase from Mycoplasmatales.

    abstract::In this report we present the first description of the isolation and partial characterization of the deoxyribonucleic acid (DNA) polymerase activity from two species of Mycoplasmatales, Mycoplasma orale type 1 and M. hyorhinis. We have identified only a single DNA polymerase species in the mycoplasma crude extracts, a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.132.2.641-649.1977

    authors: Mills LB,Stanbridge EJ,Sedwick WD,Korn D

    更新日期:1977-11-01 00:00:00

  • Deletion strains reveal metabolic roles for key elemental sulfur-responsive proteins in Pyrococcus furiosus.

    abstract::Transcriptional and enzymatic analyses of Pyrococcus furiosus previously indicated that three proteins play key roles in the metabolism of elemental sulfur (S(0)): a membrane-bound oxidoreductase complex (MBX), a cytoplasmic coenzyme A-dependent NADPH sulfur oxidoreductase (NSR), and sulfur-induced protein A (SipA). D...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05445-11

    authors: Bridger SL,Clarkson SM,Stirrett K,DeBarry MB,Lipscomb GL,Schut GJ,Westpheling J,Scott RA,Adams MW

    更新日期:2011-12-01 00:00:00