An O-antigen processing function for Wzx (RfbX): a promising candidate for O-unit flippase.

Abstract:

:O antigen is the major cell surface antigen of gram-negative bacteria, and the genes responsible for its synthesis are located in a single gene cluster. The wzx (rbfX) gene, which is characteristic of the major class of O-antigen gene clusters, encodes a hydrophobic protein with 12 potential transmembrane segments. We demonstrate that a wzx mutant accumulates undecaprenol pyrophosphate-linked O units which appear to be on the cytoplasmic side of the cytoplasmic membrane, suggesting that the wzx gene encodes a flippase for O-unit translocation across that membrane.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Liu D,Cole RA,Reeves PR

doi

10.1128/jb.178.7.2102-2107.1996

subject

Has Abstract

pub_date

1996-04-01 00:00:00

pages

2102-7

issue

7

eissn

0021-9193

issn

1098-5530

journal_volume

178

pub_type

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