An O-antigen processing function for Wzx (RfbX): a promising candidate for O-unit flippase.


:O antigen is the major cell surface antigen of gram-negative bacteria, and the genes responsible for its synthesis are located in a single gene cluster. The wzx (rbfX) gene, which is characteristic of the major class of O-antigen gene clusters, encodes a hydrophobic protein with 12 potential transmembrane segments. We demonstrate that a wzx mutant accumulates undecaprenol pyrophosphate-linked O units which appear to be on the cytoplasmic side of the cytoplasmic membrane, suggesting that the wzx gene encodes a flippase for O-unit translocation across that membrane.


J Bacteriol


Journal of bacteriology


Liu D,Cole RA,Reeves PR




Has Abstract


1996-04-01 00:00:00












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    abstract::Streptococcus suis serotype 2 (S. suis 2) is an important swine and human pathogen responsible for septicemia and meningitis. A novel gene, designated atl and encoding a major autolysin of S. suis 2 virulent strain HA9801, was identified and characterized in this study. The Atl protein contains 1,025 amino acids with ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ju CX,Gu HW,Lu CP

    更新日期:2012-03-01 00:00:00

  • SepL resembles an aberrant effector in binding to a class 1 type III secretion chaperone and carrying an N-terminal secretion signal.

    abstract::Here we show that the type III secretion gatekeeper protein SepL resembles an aberrant effector protein in binding to a class 1 type III secretion chaperone (Orf12, here renamed CesL). We also show that short N-terminal fragments (≤70 amino acids) from SepL are capable of targeting fusion proteins for secretion and tr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Younis R,Bingle LE,Rollauer S,Munera D,Busby SJ,Johnson S,Deane JE,Lea SM,Frankel G,Pallen MJ

    更新日期:2010-11-01 00:00:00

  • The leucine-responsive regulatory protein, Lrp, activates transcription of the fim operon in Salmonella enterica serovar typhimurium via the fimZ regulatory gene.

    abstract::The fim operon of Salmonella enterica serovar Typhimurium encodes type 1 fimbriae. The expression of fim is controlled in response to environmental signals through a complex regulatory cascade involving the proteins FimW, FimY, and FimZ and a genetic locus, fimU, that encodes a rare arginine tRNA. We discovered that a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: McFarland KA,Lucchini S,Hinton JC,Dorman CJ

    更新日期:2008-01-01 00:00:00

  • Complete genome sequence of the strong mutator Salmonella enterica subsp. enterica serotype Heidelberg strain B182.

    abstract::In bacteria, normal mutation frequencies are mostly around 10(-10) per base pair. However, there exists natural isolates, called "mutators," that exhibit permanent mutation occurrences up to 1,000-fold greater than usual. As mutations play essential roles, particularly in the evolution of antibiotic resistance, bacter...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Le Bars H,Bousarghin L,Bonnaure-Mallet M,Jolivet-Gougeon A,Barloy-Hubler F

    更新日期:2012-07-01 00:00:00

  • 6-phosphogluconolactonase mutants of Escherichia coli and a maltose blue gene.

    abstract::Mutants lacking an enzyme of the oxidative branch of the hexose monophosphate shunt, 6-phosphogluconolactonase (pgl), have been selected as a new class of glucose-negative derivatives of a phosphoglucose isomerase (pgi) mutant. Glucose negativity is not as complete as in mutants lacking phosphoglucose isomerase and gl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kupor SR,Fraenkel DG

    更新日期:1969-12-01 00:00:00

  • Transductional analysis of Pseudomonas aeruginosa methionineless auxotrophs.

    abstract::Seventy-one methionineless and cysteineless auxotrophs of Pseudomonas aeruginosa were placed into nine groups on the basis of their growth on methionine precursors and the cross-feeding response. Transduction experiments with bacteriophage F116 indicated the presence of four linkage groups among the methionineless mut...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Calhoun DH,Feary TW

    更新日期:1969-01-01 00:00:00

  • Characterization of the gene encoding the autotrophic ATP sulfurylase from the bacterial endosymbiont of the hydrothermal vent tubeworm Riftia pachyptila.

    abstract::ATP sulfurylase is a key enzyme in the energy-generating sulfur oxidation pathways of many chemoautotrophic bacteria. The utilization of reduced sulfur compounds to fuel CO2 fixation by the still-uncultured bacterial endosymbionts provides the basis of nutrition in invertebrates, such as the tubeworm Riftia pachyptila...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Laue BE,Nelson DC

    更新日期:1994-06-01 00:00:00

  • Mobilization of Haemophilus influenzae chromosomal markers by an Escherichia coli F' factor.

    abstract::Filter matings between E. coli K-12 strains carrying an F'::Tn5,Tn9 factor with H. influenzae Rd strains gave rise to kanamycin-chloramphenicol-resistant H. influenzae strains at a frequency of approximately 10(-6). Transfer of the F' factor to H. influenzae was verified by expression of unselected markers in H. influ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Deich RA,Green BA

    更新日期:1987-05-01 00:00:00

  • Inactivation of cell-associated fructosyltransferase in Streptococcus salivarius.

    abstract::In stationary phase, 95% of the fructosyltransferase (FTase) activity of Streptococcus salivarius ATCC 25975 was found associated with the cells. Within the first 15 min after inoculation into fresh medium, the specific activity of cell-associated FTase decreased by 92% of its initial value. After this period of initi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Jacques NA,Wittenberger CL

    更新日期:1981-12-01 00:00:00

  • Pseudomonas solanacearum genes controlling both pathogenicity on tomato and hypersensitivity on tobacco are clustered.

    abstract::A pLAFR3 cosmid clone designated pVir2 containing a 25-kilobase (kb) DNA insert was isolated from a wild-type Pseudomonas solanacearum GMI1000 genomic library. This cosmid was shown to complement all but one of the nine Tn5-induced mutants which have been isolated after random mutagenesis and which have lost both path...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Boucher CA,Van Gijsegem F,Barberis PA,Arlat M,Zischek C

    更新日期:1987-12-01 00:00:00

  • Isolation and preliminary characterization of auxotrophs of a filamentous Cyanobacterium.

    abstract::Auxotrophic mutants of the filamentous cyanobacterium Anabaena variabilis were isolated by a method in which, after mutagenesis and before penicllin enrichment, mutant and wild-type cells were separated by cavitation. Auxotrophs were identified by their inability to grow on minimal medium, and they were partially char...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Currier TC,Haury JF,Wolk CP

    更新日期:1977-03-01 00:00:00

  • Interactions between exogenous deoxyribonucleic acid and membrane vesicles isolated from Bacillus subtilis 168.

    abstract::Membrane vesicles isolated from competent cultures of Bacillus subtilis 168 bound up to 20 mug of double-stranded deoxyribonucleic acid (DNA) per mg of membrane protein in the presence of ethylenediaminetetraacetate. The formation of the DNA-membrane complex was time, temperature, and pH dependent. Eighty per cent of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Joenje H,Konings WN,Venema G

    更新日期:1974-09-01 00:00:00

  • Preliminary survey of fungistatic properties of marine algae.

    abstract::Welch, Ann Marie (U. S. Veterans Administration Hospital, Durham, N. C.). Preliminary survey of fungistatic properties of marine algae. J. Bacteriol. 83:97-99. 1962-Homogenized preparations of 35 marine algae were tested for inhibitory activity against 6 pathogenic or opportunistically pathogenic fungi with saturated ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: WELCH AM

    更新日期:1962-01-01 00:00:00

  • Inhibition of thiamine transport by chloroethylthiamine in Escherichia coli.

    abstract::Chloroethylthiamine was found to inhibit an entrapment of thiamine as thiamine monophosphate by blocking thiamine monophosphokinase in the cytoplasm after thiamine was taken up by the cells of Escherichia coli. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Iwashima A,Nose Y

    更新日期:1972-12-01 00:00:00

  • Resuscitation of a defective prophage in Salmonella cocultures.

    abstract::Widely studied Salmonella enterica serovar Typhimurium strains ATCC 14028s and SL1344 harbor a cryptic ST64B prophage unable to produce infectious virions. We found that coculturing either strain with an isogenic sibling lacking the prophage leads to the appearance of active forms of the virus. Active phage originates...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Figueroa-Bossi N,Bossi L

    更新日期:2004-06-01 00:00:00

  • Rapid transient growth at low pH in the cyanobacterium Synechococcus sp.

    abstract::The thermophilic cyanobacterium Synechococcus sp. strain Y-7c-s grows at its maximum rate at a high pH (pH 8 and above) the does not show sustained growth below pH 6.5. However, rapidly growing, exponential-phase cells from high-pH cultures continued to grow rapidly for several hours after transfer to pH 6.0 or 5.0. T...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kallas T,Castenholz RW

    更新日期:1982-01-01 00:00:00

  • Tetrapyrrole biosynthesis in Rhodobacter capsulatus is transcriptionally regulated by the heme-binding regulatory protein, HbrL.

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Smart JL,Bauer CE

    更新日期:2006-02-01 00:00:00

  • Molecular cloning of TOL genes xylB and xylE in Escherichia coli.

    abstract::The xylB and xylE genes in the TOL plasmid of Pseudomonas putida mt-2, which code for benzyl alcohol dehydrogenase and catechol 2,3-oxygenase, respectively, were cloned onto plasmid pBR322 in Escherichia coli for detailed mapping. The xylB gene was mapped in a 2.9-kilobase region within the BamHI BC fragment of pTN2, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Inouye S,Nakazawa A,Nakazawa T

    更新日期:1981-03-01 00:00:00

  • Characterization of Vibrio fischeri rRNA operons and subcloning of a ribosomal DNA promoter.

    abstract::Analysis of rRNA genes in Vibrio fischeri indicates the presence of eight rRNA gene sets in this organism. It was found that the genes for 5S rRNA, 16S rRNA, and 23S rRNA are organized in operons in the following order: 5' end 16S rRNA 23S RNA 5S rRNA 3' end. Although the operons are homologous, they are not identical...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Amikam D,Kuhn J

    更新日期:1987-05-01 00:00:00

  • The hotdog thioesterase EntH (YbdB) plays a role in vivo in optimal enterobactin biosynthesis by interacting with the ArCP domain of EntB.

    abstract::In response to iron limitation, the siderophore enterobactin is synthesized and secreted by Escherichia coli. Its biosynthesis is performed by a series of enzymes encoded by the Ent gene cluster. Among the genes of this cluster, ybdB has not been implicated in enterobactin production to date. We demonstrate here an in...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Leduc D,Battesti A,Bouveret E

    更新日期:2007-10-01 00:00:00

  • Regulation of arylsulfatase synthesis by sulfur compounds in Klebsiella aerogenes.

    abstract::In Klebsiella aerogenes, arylsulfatase synthesis was repressed by inorganic sulfate, sulfite, sulfide, thiosulfate, and cysteine, but not by methionine under normal growth conditions. We isolated cysteine-requiring mutants (Cys minus), and mutants (AtsS minus, AtsR minus) in which the regulation of arylsulfatase synth...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Adachi T,Murooka Y,Harada T

    更新日期:1975-01-01 00:00:00

  • Methylation-independent and methylation-dependent chemotaxis in Rhodobacter sphaeroides and Rhodospirillum rubrum.

    abstract::In vivo and in vitro methylation, methanol production assays, and the use of specific antibodies raised against the sensory transducing protein Tar in Escherichia coli all failed to demonstrate the presence of methyl-accepting chemotaxis proteins (MCPs) in the photosynthetic bacterium Rhodobacter sphaeroides, although...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sockett RE,Armitage JP,Evans MC

    更新日期:1987-12-01 00:00:00

  • Cloning, sequencing, and characterization of the Bacillus subtilis biotin biosynthetic operon.

    abstract::A 10-kb region of the Bacillus subtilis genome that contains genes involved in biotin-biosynthesis was cloned and sequenced. DNA sequence analysis indicated that B. subtilis contains homologs of the Escherichia coli and Bacillus sphaericus bioA, bioB, bioD, and bioF genes. These four genes and a homolog of the B. spha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Bower S,Perkins JB,Yocum RR,Howitt CL,Rahaim P,Pero J

    更新日期:1996-07-01 00:00:00

  • A nine-residue synthetic propeptide enhances secretion efficiency of heterologous proteins in Lactococcus lactis.

    abstract::Lactococcus lactis, a gram-positive organism widely used in the food industry, is a potential candidate for the secretion of biologically useful proteins. We examined the secretion efficiency and capacity of L. lactis by using the Staphylococcus aureus nuclease (Nuc) as a heterologous model protein. When expressed in ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Le Loir Y,Gruss A,Ehrlich SD,Langella P

    更新日期:1998-04-01 00:00:00

  • Purification of Helicobacter pylori NCTC 11637 cytochrome bc1 and respiration with D-proline as a substrate.

    abstract::Helicobacter pylori is a microaerophilic bacterium associated with gastric inflammation and peptic ulcers. Knowledge of how pathogenic organisms produce energy is important from a therapeutic point of view. We found d-amino acid dehydrogenase-mediated electron transport from d-proline or d-alanine to oxygen via the re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Tanigawa M,Shinohara T,Nishimura K,Nagata K,Ishizuka M,Nagata Y

    更新日期:2010-03-01 00:00:00


    abstract::Welker, N. E. (Western Reserve University, Cleveland, Ohio), and L. Leon Campbell. Preferential synthesis of alpha-amylase by Bacillus stearothermophilus in the presence of 5-methyl-tryptophan. J. Bacteriol. 87:828-831. 1964.-Washed-cell suspensions of Bacillus stearothermophilus induced with pure maltose preferential...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章



    更新日期:1964-04-01 00:00:00

  • Reversibility of SOS-associated division inhibition in Escherichia coli.

    abstract::In Escherichia coli the SOS response, induced by DNA-damaging treatments, includes two systems of cell division inhibition, SfiA and SfiC, which are thought to prevent cell division by interacting with the division protein FtsZ. It is shown here that SfiA-mediated division inhibition is readily reversible, even in the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Maguin E,Lutkenhaus J,D'Ari R

    更新日期:1986-06-01 00:00:00

  • Aerobic metabolism of Streptococcus agalactiae.

    abstract::Streptococcus agalactiae cultures possess an aerobic pathway for glucose oxidation that is strongly inhibited by cyanide. The products of glucose oxidation by aerobically grown cells of S. agalactiae 50 are lactic and acetic acids, acetylmethylcarbinol, and carbon dioxide. Glucose degradation products by aerobically g...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Mickelson MN

    更新日期:1967-07-01 00:00:00

  • External loops at the C terminus of Erwinia chrysanthemi pectate lyase C are required for species-specific secretion through the out type II pathway.

    abstract::The type II secretion system (main terminal branch of the general secretion pathway) is used by diverse gram-negative bacteria to secrete extracellular proteins. Proteins secreted by this pathway are synthesized with an N-terminal signal peptide which is removed upon translocation across the inner membrane, but the si...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lindeberg M,Boyd CM,Keen NT,Collmer A

    更新日期:1998-03-01 00:00:00

  • Vibrio cholerae VibF is required for vibriobactin synthesis and is a member of the family of nonribosomal peptide synthetases.

    abstract::A 7.5-kbp fragment of chromosomal DNA downstream of the Vibrio cholerae vibriobactin outer membrane receptor, viuA, and the vibriobactin utilization gene, viuB, was recovered from a Sau3A lambda library of O395 chromosomal DNA. By analogy with the genetic organization of the Escherichia coli enterobactin gene cluster,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Butterton JR,Choi MH,Watnick PI,Carroll PA,Calderwood SB

    更新日期:2000-03-01 00:00:00