An O-antigen processing function for Wzx (RfbX): a promising candidate for O-unit flippase.


:O antigen is the major cell surface antigen of gram-negative bacteria, and the genes responsible for its synthesis are located in a single gene cluster. The wzx (rbfX) gene, which is characteristic of the major class of O-antigen gene clusters, encodes a hydrophobic protein with 12 potential transmembrane segments. We demonstrate that a wzx mutant accumulates undecaprenol pyrophosphate-linked O units which appear to be on the cytoplasmic side of the cytoplasmic membrane, suggesting that the wzx gene encodes a flippase for O-unit translocation across that membrane.


J Bacteriol


Journal of bacteriology


Liu D,Cole RA,Reeves PR




Has Abstract


1996-04-01 00:00:00












  • Comparative study of the events associated with colicin induction.

    abstract::Colicinogenic factors ColI and ColV, which have been shown to behave as sex factors, could not be induced with mitomycin C. In contrast, the ColE(1), ColE(2), and ColE(3) factors, which do not exhibit any fertility factor characteristics, are inducible by this agent. The induced production of colicins E(1), E(2), and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Herschman HR,Helinski DR

    更新日期:1967-09-01 00:00:00

  • "Position effect" on dominance in the D-serine deaminase system of Escherichia coli K-12.

    abstract::A completely stable F32 merodiploid has been isolated, which has the dsd genotype dsdA(+)dsdCx3 /F -dsdA(+)dsdC(+). The stabilization apparently does not result from an alteration in the F, or from its integration in the chromosome, but rather from a chromosomal aberration. The stable strain can exist in two forms, wh...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: McFall E

    更新日期:1967-12-01 00:00:00

  • The unique chaperone operon of Thermotoga maritima: cloning and initial characterization of a functional Hsp70 and small heat shock protein.

    abstract::The hyperthermophilic eubacterium Thermotoga maritima possesses an operon encoding an Hsp70 molecular chaperone protein and a protein with meaningful homology to the small heat shock protein family of chaperones. This represents the first demonstrated co-operon organization for these two important classes of molecular...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Michelini ET,Flynn GC

    更新日期:1999-07-01 00:00:00

  • uhp-directed, glucose 6-phosphate membrane receptor in Escherichia coli.

    abstract::Membrane vesicles were characterized for their ability to specifically bind [14C]glucose 6-phosphate. Membranes prepared from a strain carrying a ColE1 uhp hybrid plasmid showed significantly enhanced glucose 6-phosphate binding. It is hypothesized that glucose 6-phosphate binding to these membranes is due to a uhpR-d...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Goldenbaum PE,Farmer KS

    更新日期:1980-04-01 00:00:00

  • Positive regulation of the Escherichia coli glycine cleavage enzyme system.

    abstract::A new mutation in Escherichia coli, designated gcvA1, that results in noninducible expression of both gcv and a gcvT-lacZ gene fusion was isolated. A plasmid carrying the wild-type gcvA gene complemented the mutation and restored glycine-inducible gcv and gcvT-lacZ gene expression. These results suggest that gcvA enco...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Wilson RL,Steiert PS,Stauffer GV

    更新日期:1993-02-01 00:00:00

  • Genetic determinants of a nickel-specific transport system are part of the plasmid-encoded hydrogenase gene cluster in Alcaligenes eutrophus.

    abstract::Nickel-deficient (Nic-) mutants of Alcaligenes eutrophus requiring high levels of nickel ions for autotrophic growth with hydrogen were characterized. The Nic- mutants carried defined deletions in the hydrogenase gene cluster of the indigenous pHG megaplasmid. Nickel deficiency correlated with a low level of the nicke...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Eberz G,Eitinger T,Friedrich B

    更新日期:1989-03-01 00:00:00

  • Mutations that relieve nutritional repression of the Bacillus subtilis dipeptide permease operon.

    abstract::The Bacillus subtilis dciA operon encodes a dipeptide transport complex that is induced rapidly as cells enter stationary phase and initiate sporulation. Expression of this operon in growing cells is repressed by glucose, by a mixture of amino acids, and by the AbrB protein. A genetic screen was devised to identify mu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Slack FJ,Mueller JP,Sonenshein AL

    更新日期:1993-08-01 00:00:00

  • Plasmid-determined enzymatic degradation of nylon oligomers.

    abstract::The nylon oligomer (6-aminohexanoic acid cyclic dimer) degradation genes on plasmid pOAD2 of Flavobacterium sp. KI72 were cloned into Escherichia coli vector pBR322. The locus of one of the genes, the structural gene of 6-aminohexanoic acid linear oligomer hydrolase, was determined by constructing various deletion pla...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Negoro S,Taniguchi T,Kanaoka M,Kimura H,Okada H

    更新日期:1983-07-01 00:00:00

  • phs Locus of Escherichia coli, a mutation causing pleiotropic lesions in metabolism, is an rpoA allele.

    abstract::The phs mutation, which causes a pleiotropic growth defect, has been mapped and shown to be an allele of rpoA, the gene for the alpha subunit of RNA polymerase. The mutation is shown to cause a transcription defect in the arabinose operon, araBAD. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Rowland GC,Giffard PM,Booth IR

    更新日期:1985-11-01 00:00:00

  • Cloning of Escherichia coli and Pseudomonas aeruginosa phosphomannose isomerase genes and their expression in alginate-negative mutants of Pseudomonas aeruginosa.

    abstract::The phosphomannose isomerase (pmi) gene of Escherichia coli was cloned on a broad-host-range cosmid vector and expressed in Pseudomonas aeruginosa at a low level. Plasmid pAD3, which harbors the E. coli pmi gene, contains a 6.2-kilobase-pair HindIII fragment derived from the chromosome of E. coli. Subcloning produced ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Darzins A,Nixon LL,Vanags RI,Chakrabarty AM

    更新日期:1985-01-01 00:00:00

  • Deciphering morphological determinants of the helix-shaped Leptospira.

    abstract::Leptospira spp. are thin, highly motile, slow-growing spirochetes that can be distinguished from other bacteria on the basis of their unique helical shape. Defining the mechanisms by which these bacteria generate and maintain this atypical morphology should greatly enhance our understanding of the fundamental physiolo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Slamti L,de Pedro MA,Guichet E,Picardeau M

    更新日期:2011-11-01 00:00:00

  • A zinc finger protein from Candida albicans is involved in sucrose utilization.

    abstract::A sucrose-inducible alpha-glucosidase activity that hydrolyzes sucrose in Candida albicans has been demonstrated previously. The enzyme is assayable in whole cells and was inhibited by both sucrose and maltose. A C. albicans gene (CASUC1) that affects sucrose utilization and alpha-glucosidase activity was cloned by ex...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kelly R,Kwon-Chung KJ

    更新日期:1992-01-01 00:00:00

  • Characterization of the Campylobacter jejuni heptosyltransferase II gene, waaF, provides genetic evidence that extracellular polysaccharide is lipid A core independent.

    abstract::Campylobacter jejuni produces both lipooligosaccharide (LOS) and a higher-molecular-weight polysaccharide that is believed to form a capsule. The role of these surface polysaccharides in C. jejuni-mediated enteric disease is unclear; however, epitopes associated with the LOS are linked to the development of neurologic...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Oldfield NJ,Moran AP,Millar LA,Prendergast MM,Ketley JM

    更新日期:2002-04-01 00:00:00

  • Assemblies of DivIVA mark sites for hyphal branching and can establish new zones of cell wall growth in Streptomyces coelicolor.

    abstract::Time-lapse imaging of Streptomyces hyphae revealed foci of the essential protein DivIVA at sites where lateral branches will emerge. Overexpression experiments showed that DivIVA foci can trigger establishment of new zones of cell wall assembly, suggesting a key role of DivIVA in directing peptidoglycan synthesis and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hempel AM,Wang SB,Letek M,Gil JA,Flärdh K

    更新日期:2008-11-01 00:00:00

  • Heat sensitivity of Azotobacter vinelandii genetic transformation.

    abstract::Heating competent Azotobacter vinelandii at 37 or 42 degrees C resulted in a total loss of competence with no loss of viability. The transformation process was relatively insensitive to heating at either temperature once DNase-resistant DNA binding was nearly complete. Although competent and 42 degrees C-treated cells...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Doran JL,Page WJ

    更新日期:1983-07-01 00:00:00

  • Sigma factor RpoS controls alkylresorcinol synthesis through ArpR, a LysR-type regulatory protein, during encystment of Azotobacter vinelandii.

    abstract::Azotobacter vinelandii is a bacterium which undergoes a differentiation process leading to the formation of metabolically dormant cysts. During the encystment process, A. vinelandii produces alkylresorcinol lipids (ARs) that replace the membrane phospholipids and are also components of the layers covering the cyst. Th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Romero Y,Moreno S,Guzmán J,Espín G,Segura D

    更新日期:2013-04-01 00:00:00


    abstract::Levy, Stuart B. (Institut du Radium, Paris, France). Isologous interference with ultraviolet and X-ray irradiated bacteriophage T(2). J. Bacteriol. 87:1330-1338. 1964.-Qualitative and quantitative analysis of the interference capacity of an irradiated T(2) bacteriophage was made with ultraviolet and X-ray irradiation....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: LEVY SB

    更新日期:1964-06-01 00:00:00

  • Characterization of the membrane-bound succinic dehydrogenase of Micrococcus lysodeikticus.

    abstract::The occurrence of succinic dehydrogenase [succinic:(acceptor) oxidoreductase, EC] in membrane fractions of Micrococcus lysodeikticus was investigated. The enzyme could be purified 10-fold, by deoxycholate treatment. Butanol extraction of membranes yielded an active fraction, nonsedimentable at 130,000 x g for...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Pollock JJ,Linder R,Salton MR

    更新日期:1971-07-01 00:00:00


    abstract::Wood, N. P. (A. & M. College of Texas, College Station), and D. J. O'Kane. Formate-pyruvate exchange reaction in Streptococcus faecalis. I. Factor requirement for whole cells. J. Bacteriol. 87:97-103. 1964.-A factor present in plant and animal sources was found necessary for the incorporation of formate-C(14) into pyr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: WOOD NP,O'KANE DJ

    更新日期:1964-01-01 00:00:00


    abstract::Knaysi, Georges (Cornell University, Ithaca, N.Y.). Effect of temperature on the rate of germination in Bacillus cereus. J. Bacteriol. 87:619-622. 1964.-By use of the change of the spores from bright to dark, when observed in dark contrast with a phase microscope, as a criterion for the incipience (first stage) of ger...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: KNAYSI G

    更新日期:1964-03-01 00:00:00

  • Genomic fluidity of Bordetella pertussis assessed by a new method for chromosomal mapping.

    abstract::The genomic organization of Bordetella pertussis strains has been examined by using a new method. This method does not depend on the prior determination of a restriction map of the bacterial chromosome but is based on the ability to measure directly the distance between two genes. This is accomplished through the inte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Stibitz S,Yang MS

    更新日期:1997-09-01 00:00:00

  • Pleiotropic and epistatic behavior of a ring-hydroxylating oxygenase system in the polycyclic aromatic hydrocarbon metabolic network from Mycobacterium vanbaalenii PYR-1.

    abstract::Despite the considerable knowledge of bacterial high-molecular-weight (HMW) polycyclic aromatic hydrocarbon (PAH) metabolism, the key enzyme(s) and its pleiotropic and epistatic behavior(s) responsible for low-molecular-weight (LMW) PAHs in HMW PAH-metabolic networks remain poorly understood. In this study, a phenotyp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kweon O,Kim SJ,Kim DW,Kim JM,Kim HL,Ahn Y,Sutherland JB,Cerniglia CE

    更新日期:2014-10-01 00:00:00

  • Genetic analysis of the tdcABC operon of Escherichia coli K-12.

    abstract::The biodegradative threonine dehydratase (tdc) operon was mapped at 68 min on the Escherichia coli K-12 chromosome. The order of markers in the clockwise direction was dnaG uxaA tdc argG. A tdc deletion was isolated and mapped to this region of the chromosome. By using a tdcB-lacZ fusion the clockwise direction of tra...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Schweizer HP,Datta P

    更新日期:1988-11-01 00:00:00

  • Regulation of the bacterial cell wall: analysis of a mutant of Bacillus subtilis defective in biosynthesis of teichoic acid.

    abstract::Bacillus subtilis 168ts-200B is a temperature-sensitive mutant of B. subtilis 168 which grows as rods at 30 C but as irregular spheres at 45 C. Growth at the nonpermissive temperature resulted in a deficiency of teichoic acid in the cell wall. A decrease in teichoic acid synthesis coupled with the rapid turnover of th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Boylan RJ,Mendelson NH,Brooks D,Young FE

    更新日期:1972-04-01 00:00:00

  • Cytometric detection of mycobacterial surface antigens: exposure of mannosyl epitopes and of the arabinan segment of arabinomannans.

    abstract::The physical arrangement of cell envelope components leads to the exposure of selected structural motifs which in turn may influence host-parasite interactions. To gain insight into the exposed epitopes, the present study describes a flow cytometric method designed to probe defined molecules on dispersed mycobacteria....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ozanne V,Ortalo-Magne A,Vercellone A,Fournie JJ,Daffe M

    更新日期:1996-12-01 00:00:00

  • Identification of transport proteins involved in free fatty acid efflux in Escherichia coli.

    abstract::Escherichia coli has been used as a platform host for studying the production of free fatty acids (FFA) and other energy-dense compounds useful in biofuel applications. Most of the FFA produced by E. coli are found extracellularly. This finding suggests that a mechanism for transport across the cell envelope exists, y...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lennen RM,Politz MG,Kruziki MA,Pfleger BF

    更新日期:2013-01-01 00:00:00

  • Characterization of Pseudomonas aeruginosa derepressed R-plasmids.

    abstract::A genetic study of conjugal transmissibility of two R-plasmids was undertaken in Pseudomonas aeruginosa. Conjugally derepressed mutants of the R-plasmids were isolated, and examination of 11 independent mutants revealed that 10 were recessive to the wild-type transfer repressor, whereas 1 mutant was cis dominant. Cros...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Chandler PM,Krishnapillai V

    更新日期:1977-05-01 00:00:00

  • Methyl ester of hyaluronate is unable to stimulate exolipase formation by Serratia marcescens.

    abstract::Hyaluronate stimulated the formation of exolipase by Serratia marcescens. This ability was abolished when all carboxyl groups of hyaluronate were methyl esterified. Additional studies suggested that the biological inactivity of esterified hyaluronate should be ascribed to the reduced conformational order of the molecu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Jäger KE,Winkler UK

    更新日期:1979-09-01 00:00:00

  • New mutation (mmrA1) in Escherichia coli K-12 that affects minimal medium recovery and postreplication repair after UV irradiation.

    abstract::After UV irradiation, Escherichia coli uvrA mutant cells show higher survival on minimal than on rich growth medium, i.e., they show minimal-medium recovery. This effect of rich growth medium on survival is not observed in a uvrA mutant carrying an mmrA1 mutation, and the uvrA mmrA strain showed the same survival rate...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sharma RC,Sargentini NJ,Smith KC

    更新日期:1983-05-01 00:00:00

  • Chemotaxis in Borrelia burgdorferi.

    abstract::Borrelia burgdorferi is a motile spirochete which has been identified as the causative microorganism in Lyme disease. The physiological functions which govern the motility of this organism have not been elucidated. In this study, we found that motility of B. burgdorferi required an environment similar to interstitial ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Shi W,Yang Z,Geng Y,Wolinsky LE,Lovett MA

    更新日期:1998-01-01 00:00:00