An O-antigen processing function for Wzx (RfbX): a promising candidate for O-unit flippase.

abstract：:Mutational analysis of the carboxy-terminal region of Escherichia coli HlyC was performed by site-directed mutagenesis. Replacement of residue Val-127 or Lys-129 reduced the activity of HlyC to about 30 or 60%, respectively, of that of the wild type, while replacement of Gly-128 reduced the activity to less than 1% of...

journal_title：Journal of bacteriology

authors： Guzmán-Verri C,García F,Arvidson S

更新日期：1997-09-01 00:00:00

abstract：:A Boivin preparation of Brucella abortus, unlike common enterobacterial endotoxins, failed to depress water intake or increase numbers of hemolysin-producing spleen cells in mice, or to cause delayed inflammatory reactions in rabbit skin. Reactivity to the B. abortus endotoxin was found only in animals which were prev...

journal_title：Journal of bacteriology

authors： Freedman HH,Fox AE,Willis RS,Schwartz BS

更新日期：1968-02-01 00:00:00

abstract：:A 4.2-kilobase cryptic Bacteroides plasmid, pB8-51, is found in several colonic Bacteroides species. To determine whether pB8-51 is mobilized by any of the known Bacteroides conjugal elements, we constructed an Escherichia coli-Bacteroides shuttle vector, pVAL-1, which contains pB8-51. We constructed Bacteroides unifo...

journal_title：Journal of bacteriology

authors： Valentine PJ,Shoemaker NB,Salyers AA

更新日期：1988-03-01 00:00:00

abstract：:Ribosomal subunits from Chloroflexus aurantiacus were isolated and examined by sucrose gradient sedimentation, gel electrophoresis, and electron microscopy. The 30S subunits had all the characteristic structural features of other eubacterial 30S subunits. The data support the proposal that the absence of the archaebac...

journal_title：Journal of bacteriology

authors： Henderson E,Pierson BK,Lake JA

更新日期：1983-08-01 00:00:00

abstract：:Two-micrometer deoxyribonucleic acid (DNA), a circular plasmid of Saccharomyces cerevisiae, contains two nontandem repeated sequences which are inverted with respect to one another. These repeated sequences together account for 21% of the molecule length. Restriction endonuclease analysis and electron microscopy demon...

journal_title：Journal of bacteriology

authors： Livingston DM,Klein HL

更新日期：1977-01-01 00:00:00

abstract：:Sphingomonas wittichii RW1 degrades chlorinated dibenzofurans and dibenzo-p-dioxins via meta cleavage. We used inverse PCR to amplify dxnB2, a gene encoding one of three meta-cleavage product (MCP) hydrolases identified in the organism that are homologues of BphD involved in biphenyl catabolism. Purified DxnB2 catalyz...

journal_title：Journal of bacteriology

authors： Seah SY,Ke J,Denis G,Horsman GP,Fortin PD,Whiting CJ,Eltis LD

更新日期：2007-06-01 00:00:00

abstract：:The cold-tolerant bacterium Pusillimonas sp. strain T7-7 is able to utilize diesel oils (C5 to C30 alkanes) as a sole carbon and energy source. In the present study, bioinformatics, proteomics, and real-time reverse transcriptase PCR approaches were used to identify the alkane hydroxylation system present in this bact...

journal_title：Journal of bacteriology

authors： Li P,Wang L,Feng L

更新日期：2013-05-01 00:00:00

abstract：:We have recently established that the facultative phototrophic bacterium Rhodobacter sphaeroides, like the closely related Rhodobacter capsulatus species, contains both the previously characterized mobile electron carrier cytochrome c2 (cyt c2) and the more recently discovered membrane-anchored cyt cy. However, R. sph...

journal_title：Journal of bacteriology

authors： Daldal F,Mandaci S,Winterstein C,Myllykallio H,Duyck K,Zannoni D

更新日期：2001-03-01 00:00:00

abstract：:In Escherichia coli, pyrimidine-mediated regulation of upp expression occurs by UTP-sensitive selection of alternative transcriptional start sites, which produces transcripts that differ in the ability to be elongated. The upp initially transcribed region contains the sequence GATTTTTTTTG (nontemplate strand). Initiat...

journal_title：Journal of bacteriology

authors： Cheng Y,Dylla SM,Turnbough CL Jr

更新日期：2001-01-01 00:00:00

abstract：:Hyaluronate stimulated the formation of exolipase by Serratia marcescens. This ability was abolished when all carboxyl groups of hyaluronate were methyl esterified. Additional studies suggested that the biological inactivity of esterified hyaluronate should be ascribed to the reduced conformational order of the molecu...

journal_title：Journal of bacteriology

authors： Jäger KE,Winkler UK

更新日期：1979-09-01 00:00:00

abstract：UNLABELLED:Many bacteria utilize actin-like proteins to direct peptidoglycan (PG) synthesis. MreB and MreB-like proteins are thought to act as scaffolds, guiding the localization and activity of key PG-synthesizing proteins during cell elongation. Despite their critical role in viability and cell shape maintenance, ver...

journal_title：Journal of bacteriology

authors： Duan Y,Sperber AM,Herman JK

更新日期：2016-07-13 00:00:00

abstract：:Strains monosomic for chromosome I of Saccharomyces cerevisiae contain 25 to 35% fewer rRNA genes than do normal diploid strains. When these strains are repeatedly subcultured, colonies are isolated that have magnified their number of rRNA genes to the diploid amount while remaining monosomic for chromosome I. We have...

journal_title：Journal of bacteriology

authors： Kaback DB,Halvorson HO

更新日期：1978-04-01 00:00:00

abstract：:Peptidoglycan hydrolase, LytF (CwlE), was determined to be identical to YhdD (deduced cell wall binding protein) by zymography after insertional inactivation of the yhdD gene. YhdD exhibits high sequence similarity with CwlF (PapQ, LytE) and p60 of Listeria monocytogenes. The N-terminal region of YhdD has a signal seq...

journal_title：Journal of bacteriology

authors： Ohnishi R,Ishikawa S,Sekiguchi J

更新日期：1999-05-01 00:00:00

abstract：:Strains of avian pathogenic Escherichia coli (APEC), the common pathogen of avian colibacillosis, encounter reactive oxygen species (ROS) during the infection process. Superoxide dismutases (SODs), acting as antioxidant factors, can protect against ROS-mediated host defenses. Our previous reports showed that the sodA ...

journal_title：Journal of bacteriology

authors： Gao Q,Xia L,Wang X,Ye Z,Liu J,Gao S

更新日期：2019-02-25 00:00:00

abstract：:A Bacillus subtilis culture incubated anaerobically in nitrate-containing medium lost viability during the first 3 days but recovered thereafter. A flavohemoglobin mutant showed very poor survival under these conditions unless the cells were prevented from carrying out nitrate respiration. ...

journal_title：Journal of bacteriology

authors： Nakano MM

更新日期：2006-09-01 00:00:00

abstract：:A primary determinant of pathogenicity in Erwinia stewartii is the production of extracellular polysaccharide (EPS). A single mutation can abolish both EPS synthesis and pathogenicity; both properties are restored by a single cosmid clone. Subcloning and insertion analysis have defined a single positive regulatory fun...

journal_title：Journal of bacteriology

authors： Torres-Cabassa A,Gottesman S,Frederick RD,Dolph PJ,Coplin DL

更新日期：1987-10-01 00:00:00

abstract：:The maltose regulon consists of three operons controlled by a positive regulatory gene, malT. Deletions of the gene crp were introduced into strains which carried a malT-lacZ hybrid gene. From the observed reduction in beta-galactosidase activity it was concluded that the expression of malT-lacZ, and therefore of malT...

journal_title：Journal of bacteriology

authors： Chapon C

更新日期：1982-05-01 00:00:00

abstract：:Sequence flanking the bfpA locus on the enteroadherent factor plasmid of the enteropathogenic Escherichia coli (EPEC) strain B171-8 (O111:NM) was obtained to identify genes that might be required for bundle-forming pilus (BFP) biosynthesis. Deletion experiments led to the identification of a contiguous cluster of at l...

journal_title：Journal of bacteriology

authors： Sohel I,Puente JL,Ramer SW,Bieber D,Wu CY,Schoolnik GK

更新日期：1996-05-01 00:00:00

abstract：:The nucleotide sequences of the Rhodobacter sphaeroides hemA and hemT genes, encoding 5-aminolevulinic acid (ALA) synthase isozymes, were determined. ALA synthase catalyzes the condensation of glycine and succinyl coenzyme A, the first and rate-limiting step in tetrapyrrole biosynthesis. The hemA and hemT structural g...

journal_title：Journal of bacteriology

authors： Neidle EL,Kaplan S

更新日期：1993-04-01 00:00:00

abstract：:In an attempt to isolate the superoxide dismutase (SOD) gene from the anaerobic sulfate-reducing bacterium Desulfoarculus baarsii, a DNA fragment was isolated which functionally complemented an Escherichia coli mutant (sodA sodB) deficient in cytoplasmic SODs. This region carries two open reading frames with sequences...

journal_title：Journal of bacteriology

authors： Pianzzola MJ,Soubes M,Touati D

更新日期：1996-12-01 00:00:00

abstract：:Studies on the active product of pepsin digestion of rabbit immunoglobulin G (IgG), F(ab')(2) produced from antiserum against Neurospora glutamate dehydrogenase, showed it to behave in the same way, in both antienzyme and precipitation experiments, as homologous IgG. It is proposed that the inhibition of glutamate deh...

journal_title：Journal of bacteriology

authors： Roberts DB

更新日期：1967-10-01 00:00:00

abstract：:The plasmid nature of genes specifying degradation of nicotine and nicotinate in Pseudomas convexa strain 1 (Pc1) is indicated by mitomycin curing and conjugational transfer to other strains. The NIC plasmid appears to be compatible with other metabolic plasmids in Pseudomonas putida. ...

journal_title：Journal of bacteriology

authors： Thacker R,Rørvig O,Kahlon P,Gunsalus IC

更新日期：1978-07-01 00:00:00

abstract：:Bacillus subtilis JH642 and a wild strain of B. subtilis called 22a both produce an antilisterial peptide that can be purified by anion-exchange and gel filtration chromatography. Amino acid analysis confirmed that the substance was the cyclic bacteriocin subtilosin. A mutant defective in production of the substance w...

journal_title：Journal of bacteriology

authors： Zheng G,Yan LZ,Vederas JC,Zuber P

更新日期：1999-12-01 00:00:00

abstract：:Random transposon Tn5 mutagenesis of Bradyrhizobium sp. (Arachis) strain NC92, a member of the cowpea cross-inoculation group, was carried out, and kanamycin-resistant transconjugants were tested for their symbiotic phenotype on three host plants: groundnut, siratro, and pigeonpea. Two nodulation (Nod- phenotype) muta...

journal_title：Journal of bacteriology

authors： Wilson KJ,Anjaiah V,Nambiar PT,Ausubel FM

更新日期：1987-05-01 00:00:00

abstract：:Kimler, Alexander (National Institutes of Health, Bethesda, Md.). Fluorescein amine media for rapid differentiation of staphylococci. J. Bacteriol. 82:106-109. 1961.-By modifying conventional media to include blood, mannitol, and fluorescein amine, staphylococci may be isolated and identified directly. Staphylococcus ...

journal_title：Journal of bacteriology

authors： KIMLER A

更新日期：1961-07-01 00:00:00

abstract：:Tyrosine-mediated repression of aroF and tyrP was studied by inserting DNA sequences between the two adjacent TYR R boxes which, in each case, overlap the respective RNA polymerase binding sites of these genes. In both cases, repression was greatest when homologous regions of these two TYR R boxes were on the same fac...

journal_title：Journal of bacteriology

authors： Andrews AE,Dickson B,Lawley B,Cobbett C,Pittard AJ

更新日期：1991-08-01 00:00:00

abstract：:Tryptophan is an essential amino acid that is required for normal development in Chlamydia species, and tryptophan metabolism has been implicated in chlamydial persistence and tissue tropism. The ability to synthesize tryptophan is not universal among the Chlamydiaceae, but species that have a predicted tryptophan bio...

journal_title：Journal of bacteriology

authors： Akers JC,Tan M

更新日期：2006-06-01 00:00:00

abstract：:This study shows that sequential introduction of drug resistance mutations substantially increased enzyme production in Paenibacillus agaridevorans The triple mutant YT478 (rsmG Gln225→stop codon, rpsL K56R, and rpoB R485H), generated by screening for resistance to streptomycin and rifampin, expressed a 1,100-fold-lar...

journal_title：Journal of bacteriology

authors： Funane K,Tanaka Y,Hosaka T,Murakami K,Miyazaki T,Shiwa Y,Gibu S,Inaoka T,Kasahara K,Fujita N,Yoshikawa H,Hiraga Y,Ochi K

更新日期：2018-08-10 00:00:00

abstract：:Glucosylated deoxyribonucleic acid (DNA) from phages T4 and T6 competes poorly with homologous DNA causing only a slight decrease of transformation in Group H Streptococcus strain Challis. Other types of heterologous DNAs (Micrococcus luteus, Clostridium perfringens, Escherichia coli, calf thymus and non-glucosylated ...

journal_title：Journal of bacteriology

authors： Ceglowski P,Fuchs PG,Soltyk A

更新日期：1975-12-01 00:00:00

abstract：:Deoxyribonucleic acid (DNA), pulse labeled after ultraviolet irradiation of excision-defective mutants of Haemophilus influenzae, is of lower single strand molecular weight than that of unirradiated cells but approaches the size of DNA from unirradiated cells upon further incubation in growth medium. This gap-filling ...

journal_title：Journal of bacteriology

authors： Small GD

更新日期：1975-10-01 00:00:00