An O-antigen processing function for Wzx (RfbX): a promising candidate for O-unit flippase.

abstract：:The Mg-2+-Sarkosyl crystals (M band) procedure was used to study the effect of ribonuclease (RNase) A on the association of Escherichia coli deoxyribonucleic acid (DNA) with membrane. Incubation of gently prepared cell extracts with RNase results in the release of DNA from membrane. This effect appears to result from ...

journal_title：Journal of bacteriology

authors： McIntosh MA,Earhart CF

更新日期：1975-05-01 00:00:00

abstract：:Two merodiploids of Escherichia coli that contain genes for the lac operon on both chromosome and episome were tested for production of lac enzymes after growth on various carbon sources. The specific activity of beta-galactosidase (and of thiogalactoside transacetylase) was about twice that from haploid cells when gr...

journal_title：Journal of bacteriology

authors： Fowler AV

更新日期：1972-11-01 00:00:00

abstract：:To examine as randomly as possible the role of the beta-ketoacyl and acyl carrier protein (ACP) components of bacterial type II polyketide synthases (PKSs), homologs of the chain-length-factor (CLF) genes were cloned from the environmental community of microorganisms. With PCR primers derived from conserved regions of...

journal_title：Journal of bacteriology

authors： Seow KT,Meurer G,Gerlitz M,Wendt-Pienkowski E,Hutchinson CR,Davies J

更新日期：1997-12-01 00:00:00

abstract：:The opportunistic pathogen Pseudomonas aeruginosa produces multifunctional, polar, filamentous appendages termed type IV pili. Type IV pili are involved in colonization during infection, twitching motility, biofilm formation, bacteriophage infection, and natural transformation. Electrostatic surface analysis of modele...

journal_title：Journal of bacteriology

authors： van Schaik EJ,Giltner CL,Audette GF,Keizer DW,Bautista DL,Slupsky CM,Sykes BD,Irvin RT

更新日期：2005-02-01 00:00:00

abstract：:Theophylline, aminophylline, and isobutylmethylxanthine, compounds reported to be inhibitors of adenosine 3',5'-phosphate (cAMP) phosphodiesterase, prevented the alpha-factor-induced cell cycle arrest of Saccharomyces cerevisiae a cells. To determine whether the in vivo effect of these methylxanthines on yeast pheromo...

journal_title：Journal of bacteriology

authors： Liao HH,Thorner J

更新日期：1981-12-01 00:00:00

abstract：:The gene encoding the outer membrane phosphate-selective porin protein P from Pseudomonas aeruginosa was cloned into Escherichia coli. The protein product was expressed and transported to the outer membrane of an E. coli phoE mutant and assembled into functional trimers. Expression of a product of the correct molecula...

journal_title：Journal of bacteriology

authors： Siehnel RJ,Worobec EA,Hancock RE

更新日期：1988-05-01 00:00:00

abstract：:Various methods of inducing autolysis of Escherichia coli cells were investigated, some being described here for the first time. For the autolysis of growing cells only induction methods interfering with the biosynthesis of peptidoglycan were taken into consideration, whereas with harvested cells autolysis was induced...

journal_title：Journal of bacteriology

authors： Leduc M,Kasra R,van Heijenoort J

更新日期：1982-10-01 00:00:00

abstract：:Dobrogosz, Walter J. (University of Illinois, Urbana) and Ralph D. DeMoss. Pentose utilization by Pediococcus pentosaceus. J. Bacteriol. 85:1356-1364. 1963.-Data are presented which indicate that pentoses are metabolized by Pediococcus pentosaceus through the use of an inducible phosphoketolase pathway. The utilizatio...

journal_title：Journal of bacteriology

authors： DOBROGOSZ WJ,DEMOSS RD

更新日期：1963-06-01 00:00:00

abstract：:A characteristic of N2-fixing cyanobacteria in symbiotic associations appears to be release of N2-derived NH4+. The specific activity of the primary ammonium-assimilating enzyme, glutamine synthetase (GS), was found to be three- to fourfold lower in Nostoc sp. strain 7801 grown in symbiotic association with the bryoph...

journal_title：Journal of bacteriology

authors： Joseph CM,Meeks JC

更新日期：1987-06-01 00:00:00

abstract：:Transferable plasmids play an important role in the dissemination of clindamycin-erythromycin resistance in Bacteroides fragilis. We previously described the isolation and properties of pBFTM10, a 14.9-kb ClnR transfer factor from B. fragilis TMP10. We also reported the isolation of a transfer-deficient deletion deriv...

journal_title：Journal of bacteriology

authors： Hecht DW,Jagielo TJ,Malamy MH

更新日期：1991-12-01 00:00:00

abstract：:Mutation of a conserved His-157 in the second pore loop of KtrB drastically reduced the activity of the K+ transporter from Synechocystis sp. strain PCC 6803. This result suggests that His-157 plays an essential role in the K+ transport activity of the transporter system. ...

journal_title：Journal of bacteriology

authors： Zulkifli L,Uozumi N

更新日期：2006-11-01 00:00:00

abstract：:The conditions for the infection of Bacillus stearothermophilus 4S with TP-1C phage deoxyribonucleic acid (DNA) are described. Cells from log-phase cultures are the most susceptible to phage DNA infection (transfection). A cellular component (competence factor) which enhances transfection is released into the culture ...

journal_title：Journal of bacteriology

authors： Streips UN,Welker NE

更新日期：1971-06-01 00:00:00

abstract：:The 2,647-bp nucleotide sequence of cryptic plasmid pTD1, isolated from the oral spirochete Treponema denticola, was determined. The sequence revealed two open reading frames, A and B, which encode polypeptides of 335 and 235 amino acids, respectively. Open reading frame A shows sequence similarity to genes that encod...

journal_title：Journal of bacteriology

authors： MacDougall J,Margarita D,Saint Girons I

更新日期：1992-04-01 00:00:00

abstract：:Treick, R. W. (Indiana University, Bloomington), and W. A. Konetzka. Physiological state of Escherichia coli and the inhibition of deoxyribonucleic acid synthesis by phenethyl alcohol. J. Bacteriol. 88:1580-1584. 1964.-The effects of concentration of phenethyl alcohol (PEA) and the physiological state of the cells on ...

journal_title：Journal of bacteriology

authors： TREICK RW,KONETZKA WA

更新日期：1964-12-01 00:00:00

abstract：:The nif promoters of Klebsiella pneumoniae must be activated by proteins bound to upstream sequences which are thought to interact with the sigma54-RNA polymerase holoenzyme by DNA looping. NifA is the activator for most of the promoters, and integration host factor (IHF) mediates the DNA looping. While NtrC is the ac...

journal_title：Journal of bacteriology

authors： Cheema AK,Choudhury NR,Das HK

更新日期：1999-09-01 00:00:00

abstract：:Phosphate import is required for the growth of mycobacteria and is regulated by environmental inorganic phosphate (P(i)) concentrations, although the mechanism of this regulation has not been characterized. The expression of genes involved in P(i) acquisition is frequently regulated by two-component regulatory systems...

journal_title：Journal of bacteriology

authors： Glover RT,Kriakov J,Garforth SJ,Baughn AD,Jacobs WR Jr

更新日期：2007-08-01 00:00:00

abstract：:Regulation of isoleucine, valine, and leucine biosynthesis and isoleucyl-, valyl-, and leucyl-transfer ribonucleic acid (tRNA) synthetase formation was examined in two mutant strains of Escherichia coli. One mutant was selected for growth resistance to the isoleucine analogue, ketomycin, and the other was selected for...

journal_title：Journal of bacteriology

authors： Jackson J,Williams LS,Umbarger HE

更新日期：1974-12-01 00:00:00

abstract：:A reaction center H- strain (RCH-) of Rhodobacter sphaeroides, PUHA1, was made by in vitro deletion of an XhoI restriction endonuclease fragment from the puhA gene coupled with insertion of a kanamycin resistance gene cartridge. The resulting construct was delivered to R. sphaeroides wild-type 2.4.1, with the defectiv...

journal_title：Journal of bacteriology

authors： Sockett RE,Donohue TJ,Varga AR,Kaplan S

更新日期：1989-01-01 00:00:00

abstract：:Sensory adaptation by the chemotaxis system of Escherichia coli requires adjustments of the extent of methyl esterification of the chemotaxis receptor proteins. One mechanism utilized by E. coli to make such adjustments is to control the activity of CheB, the enzyme responsible for removing receptor methyl ester group...

journal_title：Journal of bacteriology

authors： Stewart RC,Roth AF,Dahlquist FW

更新日期：1990-06-01 00:00:00

abstract：:A general strategy for cloning the functional homologs of an Escherichia coli gene was used to clone homologs of 4.5S RNA from other bacteria. The genes encoding these homologs were selected by their ability to complement a deletion of the gene for 4.5S RNA. DNA sequences of the regions encoding the homologs were dete...

journal_title：Journal of bacteriology

authors： Brown S,Thon G,Tolentino E

更新日期：1989-12-01 00:00:00

abstract：:Evidence is presented that progametangia in both the plus and the minus mating types of Mucor mucedo can be induced by one substance, namely (-)-trisporic acid B. A method is described for the determination of the concentration of the sex factors (trisporone, trisporic acid B, trisporic acid C) in mated cultures of Mu...

journal_title：Journal of bacteriology

authors： Van den Ende H,Wiechmann AH,Reyngoud DJ,Hendriks T

更新日期：1970-02-01 00:00:00

abstract：:Thiamine pyrophosphate (TPP) is a required cofactor in Salmonella typhimurium that is generated de novo by the condensation of 4-amino-5-hydroxymethyl pyrimidine (HMP) pyrophosphate and 4-methyl-5-(beta-hydroxyethyl)-thiazole (THZ) monophosphate. The THZ and HMP moieties are independently synthesized, and labeling stu...

journal_title：Journal of bacteriology

authors： Webb E,Claas K,Downs DM

更新日期：1997-07-01 00:00:00

abstract：:Necrotic enteritis (NE) is an economically important disease of poultry caused by certain Clostridium perfringens type A strains. NE pathogenesis involves the NetB toxin, which is encoded on a large conjugative plasmid within a 42-kb pathogenicity locus. Recent multilocus sequence type (MLST) studies have identified t...

journal_title：Journal of bacteriology

authors： Lepp D,Gong J,Songer JG,Boerlin P,Parreira VR,Prescott JF

更新日期：2013-03-01 00:00:00

abstract：:Chorismate mutase from Streptomyces aureofaciens was purified 12-fold. This enzyme preparation did not show any activity when tested for anthranilate synthetase, prephenate dehydrogenase, or prephenate dehydratase. The catalytic activity of chorismate mutase has a broad optimum between pH 7 and 8. The initial velocity...

journal_title：Journal of bacteriology

authors： Görisch H,Lingens F

更新日期：1973-05-01 00:00:00

abstract：:TolB is a periplasmic protein of the cell envelope Tol complex. It is partially membrane associated through an interaction with the outer membrane lipoprotein PAL (peptidoglycan-associated lipoprotein), which also belongs to the Tol system. The interaction of TolB with outer membrane porins of Escherichia coli was inv...

journal_title：Journal of bacteriology

authors： Rigal A,Bouveret E,Lloubes R,Lazdunski C,Benedetti H

更新日期：1997-12-01 00:00:00

abstract：:The flavodoxins constitute a highly conserved family of small, acidic electron transfer proteins with flavin mononucleotide prosthetic groups. They are found in prokaryotes and in red and green algae, where they provide electrons at low potentials for the reduction of nitrogen by nitrogenase, for the light-dependent r...

journal_title：Journal of bacteriology

authors： Osborne C,Chen LM,Matthews RG

更新日期：1991-03-01 00:00:00

abstract：:For the transformation of the yeast species Kluyveromyces fragilis, we have constructed a vector containing a bacterial kanamycin resistance (Kmr) gene, the TRP1 gene of Saccharomyces cerevisiae, and an autonomously replicating sequence of Kluyveromyces lactis called KARS2 . By utilizing the method based on treatment ...

journal_title：Journal of bacteriology

authors： Das S,Kellermann E,Hollenberg CP

更新日期：1984-06-01 00:00:00

abstract：:The bacterial peptidoglycan consists of glycan chains of repeating beta-1,4-linked N-acetylglucosaminyl-N-acetylmuramyl units cross-linked through short peptide chains. The polymerization of the glycans, or glycosyltransfer (GT), and transpeptidation (TP) are catalyzed by bifunctional penicillin-binding proteins (PBPs...

journal_title：Journal of bacteriology

authors： Di Guilmi AM,Dessen A,Dideberg O,Vernet T

更新日期：2003-08-01 00:00:00

abstract：:Kawakami, Masaya (Gunma University, Maebashi, Japan), Nobutaka Osawa, and Susumu Mitsuhashi. Experimental salmonellosis. VII. Comparison of the immunizing effect of live vaccine and materials extracted from Salmonella enteritidis. J. Bacteriol. 92:1585-1589. 1966.-Attempts were made to isolate the factors of live vacc...

journal_title：Journal of bacteriology

authors： Kawakami M,Osawa N,Mitsuhashi S

更新日期：1966-12-01 00:00:00

abstract：:Genome comparisons have demonstrated that dramatic genetic change often underlies the emergence of new bacterial pathogens. Evolutionary analysis of Escherichia coli O157:H7, a pathogen that has emerged as a worldwide public health threat in the past two decades, has posited that this toxin-producing pathogen evolved ...

journal_title：Journal of bacteriology

authors： Wick LM,Qi W,Lacher DW,Whittam TS

更新日期：2005-03-01 00:00:00