Characterization of a novel Rieske-type alkane monooxygenase system in Pusillimonas sp. strain T7-7.

abstract：:The chromosomally mediated penicillinase present in three strains of Escherichia coli K-12 has been purified and characterized. Two of the strains carried the ampA gene and the third the wild-type allele. The purification involves release of the enzyme by spheroplast formation, dialysis, chromatography on sulfoethyl c...

journal_title：Journal of bacteriology

authors： Linström EB,Boman HG,Steele BB

更新日期：1970-01-01 00:00:00

abstract：:Penicillium chrysogenum is an important producer of penicillin antibiotics. A key step in their biosynthesis is the oxidative cyclization of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) to isopenicillin N by the enzyme isopenicillin N synthase (IPNS). bis-ACV, the oxidized disulfide form of ACV is, however, ...

journal_title：Journal of bacteriology

authors： Cohen G,Argaman A,Schreiber R,Mislovati M,Aharonowitz Y

更新日期：1994-02-01 00:00:00

abstract：:Arthrobacter sp. strain TAD20, a chitinolytic gram-positive organism, was isolated from the sea bottom along the Antarctic ice shell. Arthrobacter sp. strain TAD20 secretes two major chitinases, ChiA and ChiB (ArChiA and ArChiB), in response to chitin induction. A single chromosomal DNA fragment containing the genes c...

journal_title：Journal of bacteriology

authors： Lonhienne T,Mavromatis K,Vorgias CE,Buchon L,Gerday C,Bouriotis V

更新日期：2001-03-01 00:00:00

abstract：:MntH, a bacterial homolog of mammalian natural resistance associated macrophage protein 1 (Nramp1), is a primary transporter for Mn(2+) influx in Salmonella enterica serovar Typhimurium and Escherichia coli. S. enterica serovar Typhimurium MntH contributes to H(2)O(2) resistance and is important for full virulence. Co...

journal_title：Journal of bacteriology

authors： Kehres DG,Janakiraman A,Slauch JM,Maguire ME

更新日期：2002-06-01 00:00:00

abstract：:Respiratory nitrate reductase purified from the cell membrane of Escherichia coli is composed of three subunits, alpha, beta, and gamma, which are encoded, respectively, by the narG, narH, and narI genes of the narGHJI operon. The product of the narJ gene was deduced previously to be a highly charged, acidic protein w...

journal_title：Journal of bacteriology

authors： Dubourdieu M,DeMoss JA

更新日期：1992-02-01 00:00:00

abstract：:Growth of Salmonella typhimurium on acetate as a sole carbon source requires expression of the glyoxylate shunt; however, the genes for the glyoxylate shunt enzymes have not been previously identified in S. typhimurium. In this study, we isolated transposon insertions in the genes for the two unique enzymes of this pa...

journal_title：Journal of bacteriology

authors： Wilson RB,Maloy SR

更新日期：1987-07-01 00:00:00

abstract：:DNA damage and perturbations in DNA replication can induce global transcriptional responses that can help organisms repair the damage and survive. RecA is known to mediate transcriptional responses to DNA damage in several bacterial species by inactivating the repressor LexA and phage repressors. To gain insight into ...

journal_title：Journal of bacteriology

authors： Goranov AI,Kuester-Schoeck E,Wang JD,Grossman AD

更新日期：2006-08-01 00:00:00

abstract：:Formate dehydrogenase has traditionally been assumed to play an essential role in energy generation during growth on C(1) compounds. However, this assumption has not yet been experimentally tested in methylotrophic bacteria. In this study, a whole-genome analysis approach was used to identify three different formate d...

journal_title：Journal of bacteriology

authors： Chistoserdova L,Laukel M,Portais JC,Vorholt JA,Lidstrom ME

更新日期：2004-01-01 00:00:00

abstract：:By transformation of dnaA null mutant host cells that are suppressed either by an rnh mutation or by chromosomal integration of a mini-R1 plasmid, it was shown that replication of miniplasmids composed of the NR1 minimal replicon had no absolute dependence upon DnaA protein. In addition, the suppression of the dnaA nu...

journal_title：Journal of bacteriology

authors： Tang XB,Womble DD,Rownd RH

更新日期：1989-10-01 00:00:00

abstract：:Johnson, Russell C. (University of Minnesota, Minneapolis), and Palmer Rogers. Differentiation of pathogenic and saprophytic leptospires with 8-azaguanine. J. Bacteriol. 88:1618-1623. 1964.-The use of the purine analogue, 8-azaguanine, as a differential agent for the separation of pathogenic and saprophytic leptospire...

journal_title：Journal of bacteriology

authors： JOHNSON RC,ROGERS P

更新日期：1964-12-01 00:00:00

abstract：:The regulatory properties of acetohydroxy acid synthetase (AHAS), the first enzyme in the biosynthetic pathway to valine and the second in the isoleucine pathway, were investigated in the fission yeast Schizosaccharomyces pombe. The enzyme was partially purified from crude extracts by protamine sulfate treatment, ammo...

journal_title：Journal of bacteriology

authors： McDonald RA,Satyanarayana T,Kaplan JG

更新日期：1973-04-01 00:00:00

abstract：:The operons of the glp regulon encoding the glycerol metabolic enzymes of Pseudomonas aeruginosa were hitherto believed to be positively regulated by the product of the glpR regulatory gene. During nucleotide sequence analysis of the region located upstream of the previously characterized glpD gene, encoding sn-glycer...

journal_title：Journal of bacteriology

authors： Schweizer HP,Po C

更新日期：1996-09-01 00:00:00

abstract：:Bacteroides fragilis V479-1 (also designated strain 92) has previously been shown to contain a conjugative plasmid, pBF4, that specifies resistance to clindamycin (Cc). A report of inducible tetracycline (Tc) resistance in this strain suggested that this phenotype was also plasmid associated (G. Privitera et al., Natu...

journal_title：Journal of bacteriology

authors： Smith CJ,Welch RA,Macrina FL

更新日期：1982-07-01 00:00:00

abstract：:Xylose is rarely described as a component of bacterial glycans. UDP-xylose is the nucleotide-activated form necessary for incorporation of xylose into glycans and is synthesized by the decarboxylation of UDP-glucuronic acid (UDP-GlcA). Enzymes with UDP-GlcA decarboxylase activity include those that lead to the formati...

journal_title：Journal of bacteriology

authors： Coyne MJ,Fletcher CM,Reinap B,Comstock LE

更新日期：2011-10-01 00:00:00

abstract：:Optochin-resistant mutant and wild-type diaphorases were purified approximately 300-fold by a combination of batch adsorption and column chromatography with diethylaminoethyl cellulose, and were characterized with regard to their pH optima, sensitivity to optochin inhibition and heat inactivation, Michaelis constants ...

journal_title：Journal of bacteriology

authors： Kreger AS,Olsen RH

更新日期：1968-10-01 00:00:00

abstract：:Although the pervasive soil and water microorganism Pseudomonas aeruginosa demonstrates heightened sensitivity to UV radiation, this species possesses a recA gene that, based on structural and functional properties, could mediate a DNA damage-responsive regulon similar to the SOS regulon of Escherichia coli. To determ...

journal_title：Journal of bacteriology

authors： Warner-Bartnicki AL,Miller RV

更新日期：1992-03-01 00:00:00

abstract：:In gram-negative bacteria, numerous cell functions, including respiration-linked electron transport, have been ascribed to the cytoplasmic membrane. Gram-negative bacteria which use solid substrates (e.g., oxidized manganese or iron) as terminal electron acceptors for anaerobic respiration are presented with a unique ...

journal_title：Journal of bacteriology

authors： Myers CR,Myers JM

更新日期：1992-06-01 00:00:00

abstract：:The genome of the facultative ribulose monophosphate (RuMP) cycle methylotroph Bacillus methanolicus encodes two bisphosphatases (GlpX), one on the chromosome (GlpX(C)) and one on plasmid pBM19 (GlpX(P)), which is required for methylotrophy. Both enzymes were purified from recombinant Escherichia coli and were shown t...

journal_title：Journal of bacteriology

authors： Stolzenberger J,Lindner SN,Persicke M,Brautaset T,Wendisch VF

更新日期：2013-11-01 00:00:00

abstract：:Escherichia coli VC30 is a temperature-sensitive mutant which is defective in autolysis. Strain VC30 lyses at 30 degrees C when treated with beta-lactam antibiotics or D-cycloserine or when deprived of diaminiopimelic acid. The same treatments inhibit growth of the mutant at 42 degrees C but do not cause lysis. Strain...

journal_title：Journal of bacteriology

authors： Lubitz W,Harkness RE,Ishiguro EE

更新日期：1984-07-01 00:00:00

abstract：:Growing cells of Campylobacter coli and C. jejuni were naturally transformed by naked DNA without the requirement for any special treatment. Transformation frequencies for homologous chromosomal DNA were approximately 10(-3) transformants per recipient cell in C. coli and 10(-4) in C. jejuni. Maximum competence was fo...

journal_title：Journal of bacteriology

authors： Wang Y,Taylor DE

更新日期：1990-02-01 00:00:00

abstract：:The copy number of 2 mu DNA-derived plasmids in CIR+ Saccharomyces cerevisiae transformants is determined by its selective marker and is usually much lower than that of the endogenous plasmid. Only plasmids containing the leu2 allele of pJDB219, designated as leu2-d, under selective conditions displayed a higher copy ...

journal_title：Journal of bacteriology

authors： Erhart E,Hollenberg CP

更新日期：1983-11-01 00:00:00

abstract：:We have mapped a regulatory site mediating the hyperproduction of cholera toxin in mutants of Vibrio cholerae strain 569B. Mutations in this locus, called htx, result in the hypertoxinogenic phenotype, as measured by the ganglioside filter assay and immunoradial diffusion. Transposon-facilitated recombination was used...

journal_title：Journal of bacteriology

authors： Mekalanos JJ,Sublett RD,Romig WR

更新日期：1979-09-01 00:00:00

abstract：:When cells of two auxotrophic mutants of Sulfolobus acidocaldarius are mixed and incubated on solid medium, they form stable genetic recombinants which can be selected, enumerated, and characterized. Any of a variety of auxotrophic markers can recombine with each other, and the phenomenon has been observed at temperat...

journal_title：Journal of bacteriology

authors： Grogan DW

更新日期：1996-06-01 00:00:00

abstract：:From citrate-utilizing (Cit+) Escherichia coli strain C53 of bovine origin, strains C53A and C53B were obtained. Upon mating with recA+ but not with recA mutant recipients of K-12, C53A produced chromosomal recombinants at quite high frequencies, leading to the following conclusions: (i) C53A is an Hfr strain; (ii) th...

journal_title：Journal of bacteriology

authors： Ishiguro N,Oka C,Asagi M,Sato G,Yoshikawa M

更新日期：1980-08-01 00:00:00

abstract：UNLABELLED:The σE envelope stress response is an essential signal transduction pathway which detects and removes mistargeted outer membrane (OM) β-barrel proteins (OMPs) in the periplasm of Escherichia coli It relies on σE, an alternative sigma factor encoded by the rpoE gene. Here we report a novel mutation, a nucleot...

journal_title：Journal of bacteriology

authors： Konovalova A,Schwalm JA,Silhavy TJ

更新日期：2016-08-11 00:00:00

abstract：:A psychrotolerant marine bacterial strain, designated JUB59(T), was isolated from Antarctic surface seawater and classified as a new species of the genus Bizionia. Here, we present the first draft genome sequence for this genus, which suggests interesting features such as UV resistance, hydrolytic exoenzymes, and nitr...

journal_title：Journal of bacteriology

authors： Lanzarotti E,Pellizza L,Bercovich A,Foti M,Coria SH,Vazquez SC,Ruberto L,Hernández EA,Dias RL,Mac Cormack WP,Cicero DO,Smal C,Nicolas MF,Vasconcelos AT,Marti MA,Turjanski AG

更新日期：2011-12-01 00:00:00

abstract：:Biofilm formation was repressed by glucose in several species of Enterobacteriaceae. In Escherichia coli, this effect was mediated at least in part by cyclic AMP (cAMP)-cAMP receptor protein. A temporal role for cAMP in biofilm development was indicated by the finding that glucose addition after approximately 24 h fai...

journal_title：Journal of bacteriology

authors： Jackson DW,Simecka JW,Romeo T

更新日期：2002-06-01 00:00:00

abstract：:The P fimbrial gene clusters encoding the serologically different F7(1), F7(2), F9, and F11 fimbriae were compared functionally. The results show that these gene clusters are closely related. ...

journal_title：Journal of bacteriology

authors： Van Die I,Van Megen I,Zuidweg E,Hoekstra W,De Ree H,Van den Bosch H,Bergmans H

更新日期：1986-07-01 00:00:00

abstract：:Sinorhizobium meliloti is a soil-dwelling alphaproteobacterium that engages in a nitrogen-fixing root nodule symbiosis with leguminous plants. Cell surface polysaccharides are important both for adapting to stresses in the soil and for the development of an effective symbiotic interaction. Among the polysaccharides ch...

journal_title：Journal of bacteriology

authors： Barnett MJ,Long SR

更新日期：2018-01-10 00:00:00

abstract：:Differential expression of genes within the puf photosynthesis operon of Rhodobacter capsulatus is achieved primarily through marked segmental differences in stability within the polycistronic puf operon transcripts. The comparatively stable pufBA segment of these transcripts outlives the labile pufLMX segment and acc...

journal_title：Journal of bacteriology

authors： Chen CY,Belasco JG

更新日期：1990-08-01 00:00:00