Abstract:
:Human-pathogenic Bartonella henselae causes cat scratch disease and vasculoproliferative disorders. An important pathogenicity factor of B. henselae is the trimeric autotransporter adhesin (TAA) Bartonella adhesin A (BadA), which is modularly constructed, consisting of a head, a long and repetitive neck-stalk module, and a membrane anchor. BadA is involved in bacterial autoagglutination, binding to extracellular matrix proteins and host cells, and in proangiogenic reprogramming. The slow growth of B. henselae and limited tools for genetic manipulation are obstacles for detailed examination of BadA and its domains. Here, we established a recombinant expression system for BadA mutants in Escherichia coli allowing functional analysis of particular BadA domains. Using a BadA mutant lacking 21 neck-stalk repeats (BadA HN23), the BadA HN23 signal sequence was exchanged with that of E. coli OmpA, and the BadA membrane anchor was additionally replaced with that of Yersinia adhesin A (YadA). Constructs were cloned in E. coli, and hybrid protein expression was detected by immunoblotting, fluorescence microscopy, and flow cytometry. Functional analysis revealed that BadA hybrid proteins mediate autoagglutination and binding to collagen and endothelial cells. In vivo, expression of this BadA construct correlated with higher pathogenicity of E. coli in a Galleria mellonella infection model.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Schmidgen T,Kaiser PO,Ballhorn W,Franz B,Göttig S,Linke D,Kempf VAdoi
10.1128/JB.01461-13subject
Has Abstractpub_date
2014-06-01 00:00:00pages
2155-65issue
12eissn
0021-9193issn
1098-5530pii
JB.01461-13journal_volume
196pub_type
杂志文章abstract::The lprG-Rv1410c operon is critical for the survival of Mycobacterium tuberculosis during infection, but very little is known about the functions of its proteins. LprG is a lipoprotein, and Rv1410c encodes the major facilitator superfamily small molecule transporter P55. P55 likely exports small molecules outside of t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01046-07
更新日期:2008-03-01 00:00:00
abstract::Members of all four families of methanogenic bacteria were analyzed for polyamine concentrations. High-performance liquid chromatography analysis of dansylated cell extracts revealed typical polyamine patterns for each family. Members of Methanobacteriaceae (family I) were characterized by very low polyamine concentra...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.3.1315-1322.1983
更新日期:1983-06-01 00:00:00
abstract::In Neurospora crassa, the expression of nit-3, the structural gene which encodes nitrate reductase, is highly regulated and requires both nitrate induction and nitrogen catabolite derepression. The major nitrogen regulatory gene, nit-2, acts in a positive fashion to turn on the expression of nit-3 and other nitrogen-r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.2.657-661.1988
更新日期:1988-02-01 00:00:00
abstract::When carbon sources become limiting for growth, bacteria must choose which of the remaining nutrients should be used first. We have identified a nutrient-sensing signaling network in Escherichia coli that is activated at the transition to stationary phase. The network is composed of the two histidine kinase/response r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01554-14
更新日期:2014-06-01 00:00:00
abstract::Emulsan, the polyanionic emulsifying agent synthesized by Acinetobacter calcoaceticus RAG-1, was depolymerized by an enzyme obtained from a soil bacterium YUV-1. The extracellular emulsan depolymerase was produced when strains RAG-1 and YUV-1 were grown together on agar medium. The enzyme was extracted from the agar a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.1.161-167.1983
更新日期:1983-10-01 00:00:00
abstract::We constructed a conditional mutant of pbpA in which transcription of the gene was placed under the control of an IPTG (isopropyl-beta-D-thiogalactopyranoside)-inducible promoter in order to explore the role of PBP1 in growth, cell wall structure, and cell division. A methicillin-resistant strain and an isogenic methi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00044-07
更新日期:2007-05-01 00:00:00
abstract::The galP1 promoter is responsible for galactose-dependent, glucose-sensitive transcription of the galactose utilization operon of Streptomyces coelicolor and Streptomyces lividans. We describe the characterization of mutations that were positioned directly upstream of the apparent transcription start site of galP1 and...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.5.1213-1220.1993
更新日期:1993-03-01 00:00:00
abstract::In Rhodobacter sphaeroides, MreB, MreC, MreD, PBP2, and RodA are encoded at the same locus. The localizations of PBP2, MreB, and MreC, which have all been implicated in the synthesis of the peptidoglycan layer, were investigated under different growth conditions to gain insight into the relationships between these pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.5.1691-1700.2006
更新日期:2006-03-01 00:00:00
abstract::It was previously reported that unlike the other obg/cgtA GTPases, the Vibrio harveyi cgtAV is not essential. Here we show that cgtAV was not disrupted in these studies and is, in fact, essential for viability. Depletion of CgtAV did not result in cell elongation. CgtAV is associated with the large ribosomal particle....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.3.1205-1210.2006
更新日期:2006-02-01 00:00:00
abstract::Biphenyl metabolism in Aspergillus toxicarius occurs by successive hydroxylations in the 4- and 4'-positions, followed by conjugation with sulfate to produce 4-hydroxybiphenyl-O-sulfonic acid and 4,4'-dihydroxybiphenyl-O-sulfonic acid. The hydroxylation reactions normally occur only after a prolonged lag period after ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.1.49-57.1983
更新日期:1983-10-01 00:00:00
abstract::Thermococcus sp. strain 4557 is a hyperthermophilic anaerobic archaeon isolated from the deep-sea hydrothermal vent Guaymas Basin site in the Gulf of California at a depth of 2,000 m. Here, we present the complete genome sequence of Thermococcus sp. 4557, which consists of a single circular chromosome of 2,011,320 bp ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05851-11
更新日期:2011-10-01 00:00:00
abstract::A gene specifying the enzyme nicotinamide deamidase has been mapped, by the interrupted mating technique, at about 33 min on the chromosome of Escherichia coli K-12. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.101.3.1090-1091.1970
更新日期:1970-03-01 00:00:00
abstract::The nonstarter lactic acid bacterium Lactococcus raffinolactis is prevalent in a wide range of environments, such as the dairy environment, but little is known about this species. Here, we present the draft genome of Lactococcus raffinolactis strain 4877, isolated from a natural mesophilic dairy starter culture. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01697-12
更新日期:2012-11-01 00:00:00
abstract::The human pathogen Vibrio cholerae carries a chromosomal superintegron (SI). The SI contains an array of hundreds of gene cassettes organized in tandem which are stable under conditions when no particular stress is applied to bacteria (such as during laboratory growth). Rearrangements of these cassettes are catalyzed ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05982-11
更新日期:2012-04-01 00:00:00
abstract::Enterohemorrhagic Escherichia coli O157:H7 (EHEC) causes bloody diarrhea and hemolytic-uremic syndrome. EHEC encodes the sRNA chaperone Hfq, which is important in posttranscriptional regulation. In EHEC strain EDL933, Hfq acts as a negative regulator of the locus of enterocyte effacement (LEE), which encodes most of t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06141-11
更新日期:2011-12-01 00:00:00
abstract::Brucella native haptens (NHs) extracted with hot water from smooth (S)-type B. abortus and B. melitensis were purified to high levels of serological activity and compared with the polysaccharide obtained by acid hydrolysis (PS) of the S lipopolysaccharide (S-LPS). By 13C nuclear magnetic resonance analysis, NHs showed...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.4.1070-1079.1996
更新日期:1996-02-01 00:00:00
abstract::Haight, Rodger D. (Oregon State University, Corvallis), and Richard Y. Morita. Thermally induced leakage from Vibrio marinus, an obligately psychrophilic bacterium. J. Bacteriol. 92:1388-1393. 1966.-Leakage of various cellular components into the surrounding menstruum occurred when Vibrio marinus was subjected to temp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.5.1388-1393.1966
更新日期:1966-11-01 00:00:00
abstract::A 4.2-kb plasmid (pLV22a) native to Bacteroides fragilis LV22 became fused to a transfer-deficient Bacteroides spp.-Escherichia coli shuttle vector by an inverse transposition event, resulting in a transferrable phenotype. The transfer phenotype was attributable to pLV22a, which was also capable of mobilization within...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.15.4466-4473.1995
更新日期:1995-08-01 00:00:00
abstract::We have investigated the evolutionary relationships between two facultatively anaerobic Magnetospirillum strains (AMB-1 and MGT-1) and fastidious, obligately microaerophilic species, such as Magnetospirillum magnetotacticum, using a molecular phylogenetic approach. Genomic DNA from strains MGT-1 and AMB-1 was used as ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.20.6689-6694.1993
更新日期:1993-10-01 00:00:00
abstract::Sequencing the insertion sites of 8,865 Tn917 insertions in Enterococcus faecalis strain OG1RF identified a hot spot in the replication terminus region corresponding to 6% of the genome where 65% of the transposons had inserted. In E. faecalis, Tn917 preferentially inserted at a 29-bp consensus sequence centered on TA...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.21.7280-7289.2004
更新日期:2004-11-01 00:00:00
abstract::Germination and outgrowth of ascospores of Saccharomyces cerevisiae 4579 require both methionine and adenine, whereas leucine is only required for outgrowth. The methionine requirement may be satisfied by S-adenosylmethionine, but this sulfonium compound will not substitute for adenine. Between 30 and 70 min of protei...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.131.1.63-68.1977
更新日期:1977-07-01 00:00:00
abstract::The development of competence by the dental caries pathogen Streptococcus mutans is mediated primarily through the alternative sigma factor ComX (SigX), which is under the control of multiple regulatory systems and activates the expression of genes involved in DNA uptake and recombination. Here we report that the indu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00995-13
更新日期:2014-01-01 00:00:00
abstract::Stable, metabolically active protoplasts of Staphylococcus aureus have been prepared by the use of a staphylolytic enzyme produced by Aeromonas hydrophila. Respiratory and glycolytic rates and synthesis of nucleic acids, protein, and lipid in these protoplasts, stabilized variously in 1.1 M sucrose, 0.37 M sodium succ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.115.3.746-751.1973
更新日期:1973-09-01 00:00:00
abstract::Lactococcus garvieae is the etiological agent of lactococcosis, one of the most important disease threats to the sustainability of the rainbow trout farming industry. Here, we present the draft genome sequence of Lactococcus garvieae strain 8831, isolated from diseased rainbow trout, which is composed of 2,087,276 bp ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05326-11
更新日期:2011-08-01 00:00:00
abstract::Lignin peroxidases (LiP) of Phanerochaete chrysosporium are encoded by a family of six closely related genes. Five LiP genes have been localized to the same dimorphic chromosome. In this investigation, relative transcript levels of the LiP genes were determined. Transcripts of the LiPA, LiPB, and O282 genes were at si...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.15.5036-5042.1992
更新日期:1992-08-01 00:00:00
abstract::The mechanism by which iron uptake is facilitated by the siderophore rhodotorulic acid (RA) in the yeast Rhodotorula pilimanae was investigated with radioactively labeled Fe and RA and kinetically inert, chromic-substituted RA complexes. The weight of the evidence supports a model in which RA mediates iron transport t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.1.69-74.1978
更新日期:1978-10-01 00:00:00
abstract::Analysis of strains with deletions of all or part of nif have ordered the Klebsiella pneumoniae genetic loci as thi rbt dal udk gnd rfb has nif shiA. The his-nif plasmids pRD1 and pTM4010 contain the genes gnd rfb his nif shiA. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.3.1041-1045.1979
更新日期:1979-06-01 00:00:00
abstract::Whole cells of Bacillus subtilis transported d-alanine and l-alanine by two different systems. The high-affinity system (K(m) of 1 muM and V(max) of 0.6 to 0.8 nmol/min per mg of protein) was specific for the two stereoisomers of alanine. The low-affinity system (K(m) of 10 muM for l-alanine and 20 muM for d-alanine a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.3.1085-1092.1974
更新日期:1974-12-01 00:00:00
abstract::The intervals between rounds of chromosome and minichromosome replication were measured by density shift experiments and found to be similar. Thus the minichromosome, a lambda asnA oriC bacteriophage, mostly replicates once each division cycle rather than randomly, despite its high copy number. Slight differences betw...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.1.430-433.1987
更新日期:1987-01-01 00:00:00
abstract::We have investigated a tyrosine kinase activity from Pseudomonas solanacearum, an economically important plant pathogen. In vitro incubation of membrane fractions with [gamma-32P]ATP and subsequent sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed an 85-kDa phosphoprotein. Phosphorylation of this prot...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.13.4356-4360.1992
更新日期:1992-07-01 00:00:00