Evolutionary relationships among Magnetospirillum strains inferred from phylogenetic analysis of 16S rDNA sequences.

Abstract:

:We have investigated the evolutionary relationships between two facultatively anaerobic Magnetospirillum strains (AMB-1 and MGT-1) and fastidious, obligately microaerophilic species, such as Magnetospirillum magnetotacticum, using a molecular phylogenetic approach. Genomic DNA from strains MGT-1 and AMB-1 was used as a template for amplification of the genes coding for 16S rRNA (16S rDNA) by the polymerase chain reaction. Amplified DNA fragments were sequenced (1,424 bp) and compared with sequences for M. magnetotacticum MS-1 and Magnetospirillum gryphiswaldense MSR-1. Phylogenetic analysis of the aligned 16S rDNA sequences indicated that the two new magnetic spirilla, AMB-1 and MGT-1, lie within the alpha subdivision (alpha-1) of the eubacterial group Proteobacteria and are closely related to Rhodospirillum fulvum and to several endosymbiotic bacteria. Strains AMB-1, MGT-1, and MS-1 formed a cluster, termed group I, in which they were more closely related to each other than to group II, which contained M. gryphiswaldense MSR-1. Group I strains were also physiologically distinct from strain MSR-1. Sequence alignment studies allowed elucidation of genus-specific regions of the 16S rDNA, and oligonucleotide primers complementary to two of these regions were used to develop a specific polymerase chain reaction assay for detection of magnetic spirilla in natural samples.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Burgess JG,Kawaguchi R,Sakaguchi T,Thornhill RH,Matsunaga T

doi

10.1128/jb.175.20.6689-6694.1993

subject

Has Abstract

pub_date

1993-10-01 00:00:00

pages

6689-94

issue

20

eissn

0021-9193

issn

1098-5530

journal_volume

175

pub_type

杂志文章
  • Regulatory effect of hydrogen on nitrogenase activity of the blue-green alga (cyanobacterium) Nostoc muscorum.

    abstract::Preincubation of the blue-green alga (cyanobacterium) Nostoc muscorum under an atmosphere of argon plus acetylene in the light led to a greater than fourfold increase of light-induced hydrogen evolution and to a 50% increase of acetylene reduction, as compared to cells that had not been preconditioned. The basic and t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.141.3.1037-1040.1980

    authors: Scherer S,Kerfin W,Böger P

    更新日期:1980-03-01 00:00:00

  • The LiaFSR system regulates the cell envelope stress response in Streptococcus mutans.

    abstract::Maintaining cell envelope integrity is critical for bacterial survival, including bacteria living in a complex and dynamic environment such as the human oral cavity. Streptococcus mutans, a major etiological agent of dental caries, uses two-component signal transduction systems (TCSTSs) to monitor and respond to vario...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01563-08

    authors: Suntharalingam P,Senadheera MD,Mair RW,Lévesque CM,Cvitkovitch DG

    更新日期:2009-05-01 00:00:00

  • In vitro transcription of the nitrogen fixation regulatory operon nifLA of Klebsiella pneumoniae.

    abstract::In vitro transcription from the promoter for the nitrogen fixation regulatory operon nifLA of K. pneumoniae requires four protein fractions: the core form of RNA polymerase; NTRA, an alternate sigma factor; NTRC, an auxiliary DNA-binding protein; and NTRB, a bifunctional enzyme that controls the activity of NTRC by co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.6.2876-2880.1987

    authors: Wong PK,Popham D,Keener J,Kustu S

    更新日期:1987-06-01 00:00:00

  • Analysis of Fur binding to operator sequences within the Neisseria gonorrhoeae fbpA promoter.

    abstract::The gene encoding Neisseria gonorrhoeae periplasmic binding protein FbpA contains two regions whose sequences exhibit homology with the Escherichia coli ferric uptake regulator protein (Fur) consensus binding sequence. In this study, DNase I footprinting experiments were employed to characterize the operator sequences...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.16.5020-5023.1996

    authors: Desai PJ,Angerer A,Genco CA

    更新日期:1996-08-01 00:00:00

  • Point mutations in a peptidoglycan biosynthesis gene cause competence induction in Haemophilus influenzae.

    abstract::We have identified three new Haemophilus influenzae mutations causing cells to exhibit extreme hypercompetence at all stages of growth. The mutations are in murE, which encodes the meso-diaminopimelate-adding enzyme of peptidoglycan synthesis. All are point mutations causing nonconservative amino acid substitutions, t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.12.3323-3330.2000

    authors: Ma C,Redfield RJ

    更新日期:2000-06-01 00:00:00

  • Iron transport in Mycobacterium smegmatis: Uptake of iron from ferric citrate.

    abstract::In mycobacterial growth medium 40 to 400 microM citrate was required to solubilize 2 microM 55Fe. This solubilized 55Fe was taken up into both iron-deficient and iron sufficient washed cell suspensions of Mycobacterium smegmatis and Mycobacterium bovis BCG. Although the 55Fe was taken up into the cell, the citrate was...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.149.1.131-135.1982

    authors: Messenger AJ,Ratledge C

    更新日期:1982-01-01 00:00:00

  • Fluorescein amine media for rapid differentiation of staphylococci.

    abstract::Kimler, Alexander (National Institutes of Health, Bethesda, Md.). Fluorescein amine media for rapid differentiation of staphylococci. J. Bacteriol. 82:106-109. 1961.-By modifying conventional media to include blood, mannitol, and fluorescein amine, staphylococci may be isolated and identified directly. Staphylococcus ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.82.1.106-109.1961

    authors: KIMLER A

    更新日期:1961-07-01 00:00:00

  • Germination properties as marker events characterizing later stages of Bacillus subtilis spore formation.

    abstract::At various stages during spore formation sporangia were shocked by cold treatment or with toluene, and the germination requirements of the prespores were examined. Up to 5 h after induction of sporulation (t5) germination was spontaneous; i.e., it occurred without any added germinants. After t5, during stages V and VI...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.141.2.786-792.1980

    authors: Dion P,Mandelstam J

    更新日期:1980-02-01 00:00:00

  • Rhizobium meliloti swims by unidirectional, intermittent rotation of right-handed flagellar helices.

    abstract::The 5 to 10 peritrichously inserted complex flagella of Rhizobium meliloti MVII-1 were found to form right-handed flagellar bundles. Bacteria swam at speeds up to 60 microns/s, their random three-dimensional walk consisting of straight runs and quick directional changes (turns) without the vigorous angular motion (tum...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.7.3146-3150.1987

    authors: Götz R,Schmitt R

    更新日期:1987-07-01 00:00:00

  • OmpR and LeuO positively regulate the Salmonella enterica serovar Typhi ompS2 porin gene.

    abstract::The Salmonella enterica serovar Typhi ompS2 gene codes for a 362-amino-acid outer membrane protein that contains motifs common to the porin superfamily. It is expressed at very low levels compared to the major OmpC and OmpF porins, as observed for S. enterica serovar Typhi OmpS1, Escherichia coli OmpN, and Klebsiella ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.10.2909-2920.2004

    authors: Fernández-Mora M,Puente JL,Calva E

    更新日期:2004-05-01 00:00:00

  • cis-acting sites in the transcript of the Bacillus subtilis trp operon regulate expression of the operon.

    abstract::Transcription of the trp operon of Bacillus subtilis is regulated by attenuation. A trpE'-'lacZ gene fusion preceded by the wild-type trp promoter-leader region was used to analyze regulation. Overproduction of the trp leader transcript in trans from a multicopy plasmid caused constitutive expression of the chromosoma...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.7.3080-3088.1988

    authors: Kuroda MI,Henner D,Yanofsky C

    更新日期:1988-07-01 00:00:00

  • Cloning, sequencing, and characterization of the Bacillus subtilis biotin biosynthetic operon.

    abstract::A 10-kb region of the Bacillus subtilis genome that contains genes involved in biotin-biosynthesis was cloned and sequenced. DNA sequence analysis indicated that B. subtilis contains homologs of the Escherichia coli and Bacillus sphaericus bioA, bioB, bioD, and bioF genes. These four genes and a homolog of the B. spha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.14.4122-4130.1996

    authors: Bower S,Perkins JB,Yocum RR,Howitt CL,Rahaim P,Pero J

    更新日期:1996-07-01 00:00:00

  • EbfC (YbaB) is a new type of bacterial nucleoid-associated protein and a global regulator of gene expression in the Lyme disease spirochete.

    abstract::Nearly every known species of Eubacteria encodes a homolog of the Borrelia burgdorferi EbfC DNA-binding protein. We now demonstrate that fluorescently tagged EbfC associates with B. burgdorferi nucleoids in vivo and that chromatin immunoprecipitation (ChIP) of wild-type EbfC showed it to bind in vivo to sites througho...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00252-12

    authors: Jutras BL,Bowman A,Brissette CA,Adams CA,Verma A,Chenail AM,Stevenson B

    更新日期:2012-07-01 00:00:00

  • Characterization of the nonenzymatic chloramphenicol resistance (cmlA) gene of the In4 integron of Tn1696: similarity of the product to transmembrane transport proteins.

    abstract::Integrons constitute a novel family of DNA elements which evolved by site-specific integration of discrete units between two conserved segments. On the In4 integron of Tn1696, a precisely inserted gene cassette of 1,549 bp conferring nonenzymatic chloramphenicol resistance (cmlA) is present between the streptomycin-sp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.14.4493-4502.1991

    authors: Bissonnette L,Champetier S,Buisson JP,Roy PH

    更新日期:1991-07-01 00:00:00

  • Subcellular sites involved in lipid synthesis in Saccharomyces cerevisiae.

    abstract::When the crude ribosomal fraction of Saccharomyces cerevisiae was separated into "light" and "heavy" fractions, fatty acid synthetase was concentrated in the former, whereas acetyl-Coenzyme A synthetase, fatty acid "desaturase," and squalene oxidocyclase were found in the latter. The "desaturase" sedimented with the r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.1.61-65.1967

    authors: Klein HP,Volkmann CM,Leaffer MA

    更新日期:1967-07-01 00:00:00

  • Expression of glycosidase activities during germination of Dictyostelium discoideum spores.

    abstract::Several lysosomal glycosidase activities were examined in vitro during heat-induced germination of Dictyostelium discoideum spores and were found not to be coordinately controlled. The level of beta-glucosidase activity increased significantly during the emergence stage of germination. Both alpha-glucosidase and N-ace...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.141.2.436-442.1980

    authors: Tisa LS,Cotter DA

    更新日期:1980-02-01 00:00:00

  • Streptococcus faecium mutants that are temperature sensitive for cell growth and show alterations in penicillin-binding proteins.

    abstract::The penicillin-binding proteins (PBPs) of 209 cell division (or growth) temperature-sensitive mutants of Streptococcus faecium were analyzed in this study. A total of nine strains showed either constitutive or temperature-sensitive conditional damage in the PBPs. Analysis of these nine strains yielded the following re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.6.2432-2439.1987

    authors: Canepari P,Lleò MM,Fontana R,Satta G

    更新日期:1987-06-01 00:00:00

  • Comparative analysis of proteins induced by heat shock, salinity, and osmotic stress in the nitrogen-fixing cyanobacterium Anabaena sp. strain L-31.

    abstract::Heat, salinity, or osmotic stress influenced protein synthesis in nitrogen-fixing Anabaena sp. strain L-31. Salinity and osmotic stresses were identical and specifically induced 15 polypeptides. Four polypeptides were unique to heat shock, and four other polypeptides were induced under every stress. The results demons...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.9.5187-5189.1989

    authors: Bhagwat AA,Apte SK

    更新日期:1989-09-01 00:00:00

  • Bacteria of the genus Bacillus have a hydrolase stereospecific to the D isomer of benzoyl-arginine-p-nitroanilide.

    abstract::A stereospecific enzyme activity capable of cleaving the amide bond of the synthetic substrate N-benzoyl-D-arginine-p-nitroanilide (D-BAPA) has been found in all aerobic and anaerobic members of the family Bacillaceae tested by us. Cells of nonsporeforming gram-positive or gram-negative bacteria contain a hydrolase ac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.12.5895-5900.1988

    authors: Gofshtein-Gandman LV,Keynan A,Milner Y

    更新日期:1988-12-01 00:00:00

  • Exopolysaccharides produced by Streptococcus mutans glucosyltransferases modulate the establishment of microcolonies within multispecies biofilms.

    abstract::Streptococcus mutans is a key contributor to the formation of the extracellular polysaccharide (EPS) matrix in dental biofilms. The exopolysaccharides, which are mostly glucans synthesized by streptococcal glucosyltransferases (Gtfs), provide binding sites that promote accumulation of microorganisms on the tooth surfa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01649-09

    authors: Koo H,Xiao J,Klein MI,Jeon JG

    更新日期:2010-06-01 00:00:00

  • Use of bacteriophage transposon Mu d1 to determine the orientation for three proC-linked phosphate-starvation-inducible (psi) genes in Escherichia coli K-12.

    abstract::We have previously used the bacteriophage transposon Mu d1 (which encodes the lacZY structural genes but without their promoter) to construct strains that have lacZY fused to phosphate-regulated promoters in Escherichia coli K-12. Among 18 identified phosphate-starvation-inducible (psi) genes, three (the phoA and two ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.146.1.93-101.1981

    authors: Wanner BL,Wieder S,McSharry R

    更新日期:1981-04-01 00:00:00

  • Topology of the membrane protein LamB by epitope tagging and a comparison with the X-ray model.

    abstract::We previously developed a genetic approach to study, with a single antibody, the topology of the outer membrane protein LamB, an Escherichia coli porin with specificity towards maltodextrins and a receptor for bacteriophage lambda. Our initial procedure consisted of inserting at random the same reporter epitope (the C...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.12.3447-3456.1996

    authors: Newton SM,Klebba PE,Michel V,Hofnung M,Charbit A

    更新日期:1996-06-01 00:00:00

  • Cells of Escherichia coli contain a protein-tyrosine kinase, Wzc, and a phosphotyrosine-protein phosphatase, Wzb.

    abstract::Two proteins of Escherichia coli, termed Wzc and Wzb, were analyzed for their capacity to participate in the reversible phosphorylation of proteins on tyrosine. First, Wzc was overproduced from its specific gene and purified to homogeneity by affinity chromatography. Upon incubation in the presence of radioactive ATP,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.11.3472-3477.1999

    authors: Vincent C,Doublet P,Grangeasse C,Vaganay E,Cozzone AJ,Duclos B

    更新日期:1999-06-01 00:00:00

  • Glutamate transport in Escherichia coli K-12: nonidentity of carriers mediating entry and exit.

    abstract::The exit of glutamate from Escherichia coli K-12 cells preloaded with the radioactive amino acid and its relation to the reaction of entry were studied. Experiments with cells preloaded to different intracellular concentrations of radioactive glutamate confirmed our earlier conclusion that glutamate exit was a first-o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.1.51-57.1973

    authors: Halpern YS,Barash H,Druck K

    更新日期:1973-01-01 00:00:00

  • EspF of enteropathogenic Escherichia coli binds sorting nexin 9.

    abstract::EspF of enteropathogenic Escherichia coli targets mitochondria and subverts a number of cellular functions. EspF consists of six putative Src homology 3 (SH3) domain binding motifs. In this study we identified sorting nexin 9 (SNX9) as a host cell EspF binding partner protein, which binds EspF via its amino-terminal S...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.8.3110-3115.2006

    authors: Marchès O,Batchelor M,Shaw RK,Patel A,Cummings N,Nagai T,Sasakawa C,Carlsson SR,Lundmark R,Cougoule C,Caron E,Knutton S,Connerton I,Frankel G

    更新日期:2006-04-01 00:00:00

  • Proteolytic cleavage of the FlhB homologue YscU of Yersinia pseudotuberculosis is essential for bacterial survival but not for type III secretion.

    abstract::Pathogenic Yersinia species employ a type III secretion system (TTSS) to target antihost factors, Yop proteins, into eukaryotic cells. The secretion machinery is constituted of ca. 20 Ysc proteins, nine of which show significant homology to components of the flagellar TTSS. A key event in flagellar assembly is the swi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.16.4500-4509.2002

    authors: Lavander M,Sundberg L,Edqvist PJ,Lloyd SA,Wolf-Watz H,Forsberg A

    更新日期:2002-08-01 00:00:00

  • Secreted-protein response to sigmaU activity in Streptomyces coelicolor.

    abstract::The filamentous bacterium Streptomyces coelicolor forms an aerial mycelium as a prerequisite to sporulation, which occurs in the aerial hyphae. Uncontrolled activity of the extracytoplasmic function sigma factor sigmaU blocks the process of aerial mycelium formation in this organism. Using a green fluorescent protein ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01759-07

    authors: Gordon ND,Ottaviano GL,Connell SE,Tobkin GV,Son CH,Shterental S,Gehring AM

    更新日期:2008-02-01 00:00:00

  • Role of Na+ cycle in cell volume regulation of Mycoplasma gallisepticum.

    abstract::The mechanism for the extrusion of Na+ from Mycoplasma gallisepticum cells was examined. Na+ efflux from cells was studied by diluting 22Na+-loaded cells into an isoosmotic NaCl solution and measuring the residual 22Na+ in the cells. Uphill 22Na+ efflux was found to be glucose dependent and linear with time over a 60-...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.8.4410-4416.1989

    authors: Shirvan MH,Schuldiner S,Rottem S

    更新日期:1989-08-01 00:00:00

  • Effect of degradative plasmid CAM-OCT on responses of Pseudomonas bacteria to UV light.

    abstract::The effect of plasmid CAM-OCT on responses to UV irradiation was compared in Pseudomonas aeruginosa, in Pseudomonas putida, and in Pseudomonas putida mutants carrying mutations in UV response genes. CAM-OCT substantially increased both survival and mutagenesis in the two species. P. aeruginosa strains without CAM-OCT ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.2.975-982.1989

    authors: McBeth DL

    更新日期:1989-02-01 00:00:00

  • Comparison of the two isofunctional enol-lactone hydrolases from Acinetobacter calcoaceticus.

    abstract::The rates of thermal denaturation and the molecular weights of the two isofunctional enol-lactone hydrolases (ELH I and ELH II) of Acinetobacter calcoaceticus were determined. The molecular weights of ELH I and ELH II were found by gel filtration to be approximately 24,000 and 21,000, respectively. In crude extract at...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.106.2.369-374.1971

    authors: Katagiri M,Wheelis ML

    更新日期:1971-05-01 00:00:00