Iron transport in Mycobacterium smegmatis: Uptake of iron from ferric citrate.

Abstract:

:In mycobacterial growth medium 40 to 400 microM citrate was required to solubilize 2 microM 55Fe. This solubilized 55Fe was taken up into both iron-deficient and iron sufficient washed cell suspensions of Mycobacterium smegmatis and Mycobacterium bovis BCG. Although the 55Fe was taken up into the cell, the citrate was not. The uptake system with M. smegmatis was not inhibited by electron transport inhibitors, uncouplers of oxidative phosphorylation, or thiol reagents and was saturable with iron at approximately 35 microM. The system was independent of the iron transport systems already known to exist in M. smegmatis: i.e., the two exochelin routes of assimilation as well as the mycobactin-salicylate system. It was not induced by the presence of 400 microM citrate in the growth medium, nor did the presence of citrate in the medium affect the production of either exochelin or mycobactin.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Messenger AJ,Ratledge C

doi

10.1128/JB.149.1.131-135.1982

subject

Has Abstract

pub_date

1982-01-01 00:00:00

pages

131-5

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

149

pub_type

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