Characterization of late acyltransferase genes of Yersinia pestis and their role in temperature-dependent lipid A variation.

Abstract:

:Yersinia pestis is an important human pathogen that is maintained in flea-rodent enzootic cycles in many parts of the world. During its life cycle, Y. pestis senses host-specific environmental cues such as temperature and regulates gene expression appropriately to adapt to the insect or mammalian host. For example, Y. pestis synthesizes different forms of lipid A when grown at temperatures corresponding to the in vivo environments of the mammalian host and the flea vector. At 37 degrees C, tetra-acylated lipid A is the major form; but at 26 degrees C or below, hexa-acylated lipid A predominates. In this study, we show that the Y. pestis msbB (lpxM) and lpxP homologs encode the acyltransferases that add C12 and C(16:1) groups, respectively, to lipid IV(A) to generate the hexa-acylated form, and that their expression is upregulated at 21 degrees C in vitro and in the flea midgut. A Y. pestis deltamsbB deltalpxP double mutant that did not produce hexa-acylated lipid A was more sensitive to cecropin A, but not to polymyxin B. This mutant was able to infect and block fleas as well as the parental wild-type strain, indicating that the low-temperature-dependent change to hexa-acylated lipid A synthesis is not required for survival in the flea gut.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Rebeil R,Ernst RK,Jarrett CO,Adams KN,Miller SI,Hinnebusch BJ

doi

10.1128/JB.188.4.1381-1388.2006

keywords:

subject

Has Abstract

pub_date

2006-02-01 00:00:00

pages

1381-8

issue

4

eissn

0021-9193

issn

1098-5530

pii

188/4/1381

journal_volume

188

pub_type

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