Abstract:
:Two types of adhesive fimbriae are expressed by Actinomyces; however, the architecture and the mechanism of assembly of these structures remain poorly understood. In this study we characterized two fimbrial gene clusters present in the genome of Actinomyces naeslundii strain MG-1. By using immunoelectron microscopy and biochemical analysis, we showed that the fimQ-fimP-srtC1-fimR gene cluster encodes a fimbrial structure (designated type 1) that contains a major subunit, FimP, forming the shaft and a minor subunit, FimQ, located primarily at the tip. Similarly, the fimB-fimA-srtC2 gene cluster encodes a distinct fimbrial structure (designated type 2) composed of a shaft protein, FimA, and a tip protein, FimB. By using allelic exchange, we constructed an in-frame deletion mutant that lacks the SrtC2 sortase. This mutant produces abundant type 1 fimbriae and expresses the monomeric FimA and FimB proteins, but it does not assemble type 2 fimbriae. Thus, SrtC2 is a fimbria-specific sortase that is essential for assembly of the type 2 fimbriae. Together, our experiments pave the way for several lines of molecular investigation that are necessary to elucidate the fimbrial assembly pathways in Actinomyces and their function in the pathogenesis of different biofilm-related oral diseases.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Mishra A,Das A,Cisar JO,Ton-That Hdoi
10.1128/JB.01952-06subject
Has Abstractpub_date
2007-04-01 00:00:00pages
3156-65issue
8eissn
0021-9193issn
1098-5530pii
JB.01952-06journal_volume
189pub_type
杂志文章abstract::A new pathway for aerobic benzoate oxidation has been postulated for Azoarcus evansii and for a Bacillus stearothermophilus-like strain. Benzoate is first transformed into benzoyl coenzyme A (benzoyl-CoA), which subsequently is oxidized to 3-hydroxyadipyl-CoA and then to 3-ketoadipyl-CoA; all intermediates are CoA thi...
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pub_type: 杂志文章
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更新日期:2002-11-01 00:00:00
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doi:10.1128/JB.00220-07
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.6.3331-3336.1989
更新日期:1989-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.13.3934-3940.1993
更新日期:1993-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.6.1585-1589.1966
更新日期:1966-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.3.1309-1314.1983
更新日期:1983-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.6.1332-1338.1963
更新日期:1963-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1995-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00779-17
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pub_type: 杂志文章
doi:10.1128/jb.179.3.976-981.1997
更新日期:1997-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.165.2.517-522.1986
更新日期:1986-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.174.5.1619-1625.1992
更新日期:1992-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.165.3.831-836.1986
更新日期:1986-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2009-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1991-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1970-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1996-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.4.2042-2048.1989
更新日期:1989-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1970-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.10.3101-3110.2003
更新日期:2003-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.119.2.500-507.1974
更新日期:1974-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.122.3.810-817.1975
更新日期:1975-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1985-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.6.2245-2250.1966
更新日期:1966-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.6.3553-3556.1989
更新日期:1989-06-01 00:00:00
abstract:UNLABELLED:Misincorporation of D-tyrosine (D-Tyr) into cellular proteins due to mischarging of tRNA(Tyr) with D-Tyr by tyrosyl-tRNA synthetase inhibits growth and biofilm formation of Bacillus subtilis. Furthermore, many B. subtilis strains lack a functional gene encoding D-aminoacyl-tRNA deacylase, which prevents misi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00008-15
更新日期:2015-05-01 00:00:00
abstract::Clostridium difficile is the etiological agent of antibiotic-associated diarrhea. Among the factors that may play a role in infection are S-layer proteins (SLPs). Previous work has shown these to consist mainly of two components, resulting from the cleavage of a precursor encoded by the slpA gene. The high-molecular-w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.14.3886-3897.2002
更新日期:2002-07-01 00:00:00