The yjeB (nsrR) gene of Escherichia coli encodes a nitric oxide-sensitive transcriptional regulator.

Abstract:

:Microarray studies of the Escherichia coli response to nitric oxide and nitrosative stress have suggested that additional transcriptional regulators of this response remain to be characterized. We identify here the product of the yjeB gene as a negative regulator of the transcription of the ytfE, hmpA and ygbA genes, all of which are known to be upregulated by nitrosative stress. Transcriptional fusions to the promoters of these genes were expressed constitutively in a yjeB mutant, indicating that all three are targets for repression by YjeB. An inverted repeat sequence that overlaps the -10 element of all three promoters is proposed to be a binding site for the YjeB protein. A similar inverted repeat sequence was identified in the tehA promoter, which is also known to be sensitive to nitrosative stress. The ytfE, hmpA, ygbA, and tehA promoters all caused derepression of a ytfE-lacZ transcriptional fusion when present in the cell in multiple copies, presumably by a repressor titration effect, suggesting the presence of functional YjeB binding sites in these promoters. However, YjeB regulation of tehA was weak, as judged by the activity of a tehA-lacZ fusion, perhaps because YjeB repression of tehA is masked by other regulatory mechanisms. Promoters regulated by YjeB could be derepressed by iron limitation, which is consistent with an iron requirement for YjeB activity. The YjeB protein is a member of the Rrf2 family of transcriptional repressors and shares three conserved cysteine residues with its closest relatives. We propose a regulatory model in which the YjeB repressor is directly sensitive to nitrosative stress. On the basis of similarity to the nitrite-responsive repressor NsrR from Nitrosomonas europaea, we propose that the yjeB gene of E. coli be renamed nsrR.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Bodenmiller DM,Spiro S

doi

10.1128/JB.188.3.874-881.2006

keywords:

subject

Has Abstract

pub_date

2006-02-01 00:00:00

pages

874-81

issue

3

eissn

0021-9193

issn

1098-5530

pii

188/3/874

journal_volume

188

pub_type

杂志文章
  • The RNA polymerase alpha subunit from Sinorhizobium meliloti can assemble with RNA polymerase subunits from Escherichia coli and function in basal and activated transcription both in vivo and in vitro.

    abstract::Sinorhizobium meliloti, a gram-negative soil bacterium, forms a nitrogen-fixing symbiotic relationship with members of the legume family. To facilitate our studies of transcription in S. meliloti, we cloned and characterized the gene for the alpha subunit of RNA polymerase (RNAP). S. meliloti rpoA encodes a 336-amino-...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.14.3808-3814.2002

    authors: Peck MC,Gaal T,Fisher RF,Gourse RL,Long SR

    更新日期:2002-07-01 00:00:00

  • Analysis of the binding site of the LysR-type transcriptional activator TcbR on the tcbR and tcbC divergent promoter sequences.

    abstract::The TcbR transcriptional activator protein, which is encoded by the tcbR gene of Pseudomonas sp. strain P51 (J. R. van der Meer, A. C. J. Frijters, J. H. J. Leveau, R. I. L. Eggen, A. J. B. Zehnder, and W. M. de Vos, J. Bacteriol. 173:3700-3708, 1991), was purified from overproducing Escherichia coli cells by using a ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.7.1850-1856.1994

    authors: Leveau JH,de Vos WM,van der Meer JR

    更新日期:1994-04-01 00:00:00

  • The State of the Union Is Strong: a Review of ASM's 6th Conference on Cell-Cell Communication in Bacteria.

    abstract::The 6th American Society for Microbiology Conference on Cell-Cell Communication in Bacteria convened from 16 to 19 October 2017 in Athens, GA. In this minireview, we highlight some of the research presented at that meeting that addresses central questions emerging in the field, including the following questions. How a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章,评审

    doi:10.1128/JB.00291-18

    authors: Brown SP,Blackwell HE,Hammer BK

    更新日期:2018-06-25 00:00:00

  • Mechanical properties of Bacillus subtilis cell walls: effects of removing residual culture medium.

    abstract::Experiments are described in which the tensile strength, the initial (Youngs') modulus, and other mechanical properties of the bacterial cell wall were obtained as functions of relative humidity (RH) in the range of 20 to 95%. These properties were deduced from tensile tests on bacterial thread, a fiber consisting of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.1.197-203.1991

    authors: Thwaites JJ,Surana UC

    更新日期:1991-01-01 00:00:00

  • Genetic and segregation analysis of Escherichia coli strains containing a tandem duplication of the trpD-purB region of the chromosome.

    abstract::Genetic and segregation analysis of Escherichia coli strains containing a partial duplication of the trp operon reveal that the 2.5-min-long region trpD-purB is duplicated in tandem in the chromosome. The adjacent loci cysB and fabD are not duplicated. Although one copy of the duplicated region is longer than the maxi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.133.2.650-660.1978

    authors: Simonian MH,Goldstein RV,Mosteller RD

    更新日期:1978-02-01 00:00:00

  • Role of sodium in determining alternate pathways of aerobic citrate catabolism in Aerobacter aerogenes.

    abstract::In contrast to the absolute Na(+) requirement for anaerobic growth of Aerobacter aerogenes on citrate as sole carbon source, aerobic growth of this microorganism did not require the presence of Na(+). However, Na(+) (optimal concentration, 10 mm) did increase the maximal amount of aerobic growth by 60%, even though it...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.99.2.389-394.1969

    authors: O'Brien RW,Stern JR

    更新日期:1969-08-01 00:00:00

  • Using Tn-seq To Identify Pigmentation-Related Genes of Porphyromonas gingivalis: Characterization of the Role of a Putative Glycosyltransferase.

    abstract::Cellular pigmentation is an important virulence factor of the oral pathogen Porphyromonas gingivalis Pigmentation has been associated with many bacterial functions, including but not limited to colonization, maintaining a local anaerobic environment by binding oxygen molecules, and defense against reactive oxygen spec...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00832-16

    authors: Klein BA,Cornacchione LP,Collins M,Malamy MH,Duncan MJ,Hu LT

    更新日期:2017-06-27 00:00:00

  • Physiological effects of anti-TRAP protein activity and tRNA(Trp) charging on trp operon expression in Bacillus subtilis.

    abstract::The Bacillus subtilis anti-TRAP protein regulates the ability of the tryptophan-activated TRAP protein to bind to trp operon leader RNA and promote transcription termination. AT synthesis is regulated both transcriptionally and translationally by uncharged tRNA(Trp). In this study, we examined the roles of AT synthesi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01820-07

    authors: Cruz-Vera LR,Gong M,Yanofsky C

    更新日期:2008-03-01 00:00:00

  • RNase E affects the expression of the acyl-homoserine lactone synthase gene sinI in Sinorhizobium meliloti.

    abstract::Quorum sensing of Sinorhizobium meliloti relies on N-acyl-homoserine lactones (AHLs) as autoinducers. AHL production increases at high population density, and this depends on the AHL synthase SinI and two transcriptional regulators, SinR and ExpR. Our study demonstrates that ectopic expression of the gene rne, coding ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01471-13

    authors: Baumgardt K,Charoenpanich P,McIntosh M,Schikora A,Stein E,Thalmann S,Kogel KH,Klug G,Becker A,Evguenieva-Hackenberg E

    更新日期:2014-04-01 00:00:00

  • Swarming differentiation and swimming motility in Bacillus subtilis are controlled by swrA, a newly identified dicistronic operon.

    abstract::The number and disposition of flagella harbored by eubacteria are regulated by a specific trait successfully maintained over generations. The genes governing the number of flagella in Bacillus subtilis have never been identified, although the ifm locus has long been recognized to influence the motility phenotype of th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.15.5356-5366.2005

    authors: Calvio C,Celandroni F,Ghelardi E,Amati G,Salvetti S,Ceciliani F,Galizzi A,Senesi S

    更新日期:2005-08-01 00:00:00

  • Chemotaxis mutants of Spirochaeta aurantia.

    abstract::Five Spirochaeta aurantia chemotaxis mutants were isolated. One mutant (the che-101 mutant) never reversed, one (the che-200 mutant) flexed predominantly, two (the che-300 and che-400-1 mutants) exhibited elevated reversal frequencies, and one (the che-400 mutant) exhibited chemotactically unstimulated behavior simila...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.1.606-611.1989

    authors: Fosnaugh K,Greenberg EP

    更新日期:1989-01-01 00:00:00

  • Genome sequence of strain IMCC3088, a proteorhodopsin-containing marine bacterium belonging to the OM60/NOR5 clade.

    abstract::Strain IMCC3088, cultivated from the Yellow Sea, is a novel isolate belonging to the OM60/NOR5 clade and is closely related to clone OM241, Congregibacter litoralis, and strain HTCC2080. Here, the genome sequence of strain IMCC3088 is presented, showing the absence of photosynthetic gene clusters and the presence of p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05111-11

    authors: Jang Y,Oh HM,Kang I,Lee K,Yang SJ,Cho JC

    更新日期:2011-07-01 00:00:00

  • In vitro transcription of the nitrogen fixation regulatory operon nifLA of Klebsiella pneumoniae.

    abstract::In vitro transcription from the promoter for the nitrogen fixation regulatory operon nifLA of K. pneumoniae requires four protein fractions: the core form of RNA polymerase; NTRA, an alternate sigma factor; NTRC, an auxiliary DNA-binding protein; and NTRB, a bifunctional enzyme that controls the activity of NTRC by co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.6.2876-2880.1987

    authors: Wong PK,Popham D,Keener J,Kustu S

    更新日期:1987-06-01 00:00:00

  • Inoculum-dependent division lag of Bacillus cultures and its relation to an endogenous factor(s) ("schizokinen").

    abstract::Lankford, C. E. (The University of Texas, Austin), James R. Walker, James B. Reeves, N. H. Nabbut, B. R. Byers, and R. J. Jones. Inoculum-dependent division lag of Bacillus cultures and its relation to an endogenous factor(s) ("schizokinen"). J. Bacteriol. 91:1070-1079. 1966.-When cells of Bacillus megaterium, grown o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.3.1070-1079.1966

    authors: Lankford CE,Walker JR,Reeves JB,Nabbut NH,Byers BR,Jones RJ

    更新日期:1966-03-01 00:00:00

  • Molecular cloning of genes involved with expression of A-band lipopolysaccharide, an antigenically conserved form, in Pseudomonas aeruginosa.

    abstract::Most strains of Pseudomonas aeruginosa can express two chemically and immunologically distinct types of lipopolysaccharide (LPS), an antigenically conserved form called A band and the serotype-specific form called B band. To study the molecular controls regulating expression of the A-band LPS antigen, we have cloned t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.18.5624-5630.1991

    authors: Lightfoot J,Lam JS

    更新日期:1991-09-01 00:00:00

  • NtrC is required for control of Klebsiella pneumoniae NifL activity.

    abstract::In response to molecular oxygen and/or fixed nitrogen, the product of the Klebsiella pneumoniae nitrogen fixation L (nifL) gene inhibits NifA-mediated transcriptional activation. Nitrogen regulation of NifL function occurs at two levels: transcription of the nifLA operon is regulated by the general Ntr system, and the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.23.7446-7455.1997

    authors: He L,Soupene E,Kustu S

    更新日期:1997-12-01 00:00:00

  • Derivation of glycine from threonine in Escherichia coli K-12 mutants.

    abstract::Escherichia coli AT2046 has been shown previously to lack the enzyme serine transhydroxymethylase and to require exogenous glycine for growth as a consequence. Strains JEV73 and JEV73R, mutants derived from strain AT2046, are shown here to be serine transhydroxymethylase deficient, but able to derive their glycine fro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.122.3.810-817.1975

    authors: Fraser J,Newman EB

    更新日期:1975-06-01 00:00:00

  • Extracellular loops of lipid A 3-O-deacylase PagL are involved in recognition of aminoarabinose-based membrane modifications in Salmonella enterica serovar typhimurium.

    abstract::Salmonella enterica serovar Typhimurium modifies its lipopolysaccharide (LPS), including the lipid A portion, in response to changes in its environment including host tissues. The lipid A 3-O-deacylase PagL, the expression of which is promoted under a host-mimetic environment, exhibits latency in S. enterica; deacylat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00587-08

    authors: Manabe T,Kawasaki K

    更新日期:2008-08-01 00:00:00

  • Electron microscopy of Staphylococcus aureus cells and cell walls after treatment with lysozyme Chalaropsis.

    abstract::Lysozyme Chalaropsis, a beta-1,4-N,6-O-diacetylmuramidase, attacks the cell wall murein of Staphylococcus aureus H in a random manner. In hypertonic sucrose solutions, true protoplasts are produced and, in the process, mesosomal tubules are extruded into the medium. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.117.2.900-903.1974

    authors: Robinson JP,Robinson RD,Hash JH

    更新日期:1974-02-01 00:00:00

  • Quantitative measurements of proton motive force and motility in Bacillus subtilis.

    abstract::The protein motive force of metabolizing Bacillus subtilis cells was only slightly affected by changes in the external pH between 5 and 8, although the electrical component and the chemical component of the proton motive force contributed differently at different external pH. The electrical component of the proton mot...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.144.3.891-897.1980

    authors: Shioi JI,Matsuura S,Imae Y

    更新日期:1980-12-01 00:00:00

  • The tolC locus of Escherichia coli affects the expression of three major outer membrane proteins.

    abstract::tolC mutants, which are resistant to colicin E1 and also highly sensitive to detergents and dyes, were shown to lack the OmpF outer membrane protein. There was little effect on transcription as judged by the use of an ompF-lac operon fusion strain, and the tolC effect was probably due to a post-transcriptional effect....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.150.3.1016-1023.1982

    authors: Morona R,Reeves P

    更新日期:1982-06-01 00:00:00

  • Methyl ester of hyaluronate is unable to stimulate exolipase formation by Serratia marcescens.

    abstract::Hyaluronate stimulated the formation of exolipase by Serratia marcescens. This ability was abolished when all carboxyl groups of hyaluronate were methyl esterified. Additional studies suggested that the biological inactivity of esterified hyaluronate should be ascribed to the reduced conformational order of the molecu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.139.3.1065-1067.1979

    authors: Jäger KE,Winkler UK

    更新日期:1979-09-01 00:00:00

  • Role of the sfiA-dependent cell division regulation system in Escherichia coli.

    abstract::Several authors have suggested that the SOS-associated (sfiA-dependent) system of division inhibition, normally induced by perturbations of DNA replication, also regulates steady-state (unperturbed) cell division. The present work shows that mean cell mass is identical in sfiA+ and sfiA mutant cultures during steady-s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.153.2.1072-1074.1983

    authors: Huisman O,Jacques M,D'ari R,Caro L

    更新日期:1983-02-01 00:00:00

  • Reassociation of deoxyribonucleic acids from Actinoplanes and other actinomycetes.

    abstract::The ability of deoxyribonucleic acid (DNA) isolated from a number of actinomycetes to reassociate with reference DNA from Actinoplanes philippinensis or Streptomyces venezuelae has been measured. All of the DNA preparations except for those from Nocardia erythropolis and Thermomonospora viridis contained 70 to 73 mole...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.102.1.30-35.1970

    authors: Farina G,Bradley SG

    更新日期:1970-04-01 00:00:00

  • nfi, the gene for endonuclease V in Escherichia coli K-12.

    abstract::Endonuclease V is specific for single-stranded DNA or for duplex DNA that contains uracil or that is damaged by a variety of agents (B. Demple and S. Linn, J. Biol. Chem. 257:2848-2855, 1982). Thus, it may be a versatile DNA repair enzyme. The protein was purified to apparent homogeneity, and from its N-terminal seque...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.2.310-316.1997

    authors: Guo G,Ding Y,Weiss B

    更新日期:1997-01-01 00:00:00

  • Transcription of Sialic Acid Catabolism Genes in Corynebacterium glutamicum Is Subject to Catabolite Repression and Control by the Transcriptional Repressor NanR.

    abstract:UNLABELLED:Corynebacterium glutamicum metabolizes sialic acid (Neu5Ac) to fructose-6-phosphate (fructose-6P) via the consecutive activity of the sialic acid importer SiaEFGI, N-acetylneuraminic acid lyase (NanA), N-acetylmannosamine kinase (NanK), N-acetylmannosamine-6P epimerase (NanE), N-acetylglucosamine-6P deacetyl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00820-15

    authors: Uhde A,Brühl N,Goldbeck O,Matano C,Gurow O,Rückert C,Marin K,Wendisch VF,Krämer R,Seibold GM

    更新日期:2016-07-28 00:00:00

  • New role for the ibeA gene in H2O2 stress resistance of Escherichia coli.

    abstract::ibeA is a virulence factor found in some extraintestinal pathogenic Escherichia coli (ExPEC) strains from the B2 phylogenetic group and particularly in newborn meningitic and avian pathogenic strains. It was shown to be involved in the invasion process of the newborn meningitic strain RS218. In a previous work, we sho...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00089-12

    authors: Fléchard M,Cortes MA,Répérant M,Germon P

    更新日期:2012-09-01 00:00:00

  • Lactococcin A, a new bacteriocin from Lactococcus lactis subsp. cremoris: isolation and characterization of the protein and its gene.

    abstract::A new bacteriocin, termed lactococcin A (LCN-A), from Lactococcus lactis subsp. cremoris LMG 2130 was purified and sequenced. The polypeptide contained no unusual amino acids and showed no significant sequence similarity to other known proteins. Only lactococci were killed by the bacteriocin. Of more than 120 L. lacti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.12.3879-3887.1991

    authors: Holo H,Nilssen O,Nes IF

    更新日期:1991-06-01 00:00:00

  • Effects of treatment with sodium dodecyl sulfate on the ultrastructure of Escherichia coli.

    abstract::An electron microscopy study has been made of the effects of dissolution of the plasma membrane of Escherichia coli with sodium dodecyl sulfate (SDS) on the organization of the nucleoplasm and the cytoplasm. The alterations observed in time course experiments were related to absorbance changes and to release of macrom...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.111.3.801-813.1972

    authors: Woldringh CL,van Iterson W

    更新日期:1972-09-01 00:00:00

  • Complementation of a Rhodopseudomonas sphaeroides ribulose bisphosphate carboxylase-oxygenase regulatory mutant from a genomic library.

    abstract::A genomic library containing HindIII partial digests of Rhodopseudomonas sphaeroides HR DNA was constructed in the broad-host-range cosmid cloning vector pVK102. With a portion of this library as donor in complementation studies with the ribulose 1,5-bisphosphate carboxylase-oxygenase regulatory mutant R. sphaeroides ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.1.147-154.1985

    authors: Weaver KE,Tabita FR

    更新日期:1985-10-01 00:00:00